Restriction map for
pFN21AE6315
(Linear) (Six-base) MAPSORT of: KIEE6315.seq Check: 4531 from: 1 to: 1311
KIEE6315.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 923 1311
Size: 156 767 388
Fragments arranged by size:
767 388 156
AfeI AGC'GCT
Cuts at: 0 428 1311
Size: 428 883
AflIII A'CryG_T
Cuts at: 0 317 1082 1311
Size: 317 765 229
Fragments arranged by size:
765 317 229
AhdI GACnn_n'nnGTC
Cuts at: 0 995 1311
Size: 995 316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 447 1311
Size: 364 83 864
Fragments arranged by size:
864 364 83
ApaI G_GGCC'C
Cuts at: 0 88 1311
Size: 88 1223
AvaI C'yCGr_G
Cuts at: 0 148 1311
Size: 148 1163
AvrII C'CTAG_G
Cuts at: 0 699 1311
Size: 699 612
BanI G'GyrC_C
Cuts at: 0 63 267 328 1311
Size: 63 204 61 983
Fragments arranged by size:
983 204 63 61
BanII G_rGCy'C
Cuts at: 0 88 143 203 1311
Size: 88 55 60 1108
Fragments arranged by size:
1108 88 60 55
BbeI G_GCGC'C
Cuts at: 0 67 271 332 1311
Size: 67 204 61 979
Fragments arranged by size:
979 204 67 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 747 1311
Size: 747 564
BbvCI CC'TCA_GC
Cuts at: 0 586 1311
Size: 586 725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 81 115 382 416 1311
Size: 81 34 267 34 895
Fragments arranged by size:
895 267 81 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 895 1311
Size: 895 416
BglI GCCn_nnn'nGGC
Cuts at: 0 221 440 505 1311
Size: 221 219 65 806
Fragments arranged by size:
806 221 219 65
BlpI GC'TnA_GC
Cuts at: 0 142 1311
Size: 142 1169
Bme1580I G_kGCm'C
Cuts at: 0 88 1311
Size: 88 1223
BmgBI CAC'GTC
Cuts at: 0 375 396 1311
Size: 375 21 915
Fragments arranged by size:
915 375 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 1311
Size: 102 1209
Bpu10I CC'TnA_GC
Cuts at: 0 586 1311
Size: 586 725
BsaI GGTCTCn'nnnn_
Cuts at: 0 818 1311
Size: 818 493
BsaHI Gr'CG_yC
Cuts at: 0 64 268 329 752 1311
Size: 64 204 61 423 559
Fragments arranged by size:
559 423 204 64 61
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 597 1311
Size: 597 714
BseYI C'CCAG_C
Cuts at: 0 351 659 1311
Size: 351 308 652
Fragments arranged by size:
652 351 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 706 1042 1311
Size: 348 358 336 269
Fragments arranged by size:
358 348 336 269
BsiHKAI G_wGCw'C
Cuts at: 0 515 1311
Size: 515 796
Bsp1286I G_dGCh'C
Cuts at: 0 88 143 203 515 1311
Size: 88 55 60 312 796
Fragments arranged by size:
796 312 88 60 55
BspHI T'CATG_A
Cuts at: 0 596 1311
Size: 596 715
BsrBI CCG'CTC
Cuts at: 0 1297 1311
Size: 1297 14
BsrFI r'CCGG_y
Cuts at: 0 124 850 1311
Size: 124 726 461
Fragments arranged by size:
726 461 124
BsrGI T'GTAC_A
Cuts at: 0 1030 1079 1311
Size: 1030 49 232
Fragments arranged by size:
1030 232 49
BssHII G'CGCG_C
Cuts at: 0 413 1311
Size: 413 898
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 618 1311
Size: 81 537 693
Fragments arranged by size:
693 537 81
BstYI r'GATC_y
Cuts at: 0 650 1179 1311
Size: 650 529 132
Fragments arranged by size:
650 529 132
BtgI C'CryG_G
Cuts at: 0 237 306 407 503 1311
Size: 237 69 101 96 808
Fragments arranged by size:
808 237 101 96 69
EaeI y'GGCC_r
Cuts at: 0 497 1311
Size: 497 814
EarI CTCTTCn'nnn_
Cuts at: 0 34 967 1226 1311
Size: 34 933 259 85
Fragments arranged by size:
933 259 85 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 156 923 1311
Size: 102 54 767 388
Fragments arranged by size:
767 388 102 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 926 1311
Size: 926 385
EcoO109I rG'GnC_Cy
Cuts at: 0 85 865 1311
Size: 85 780 446
Fragments arranged by size:
780 446 85
HaeII r_GCGC'y
Cuts at: 0 67 125 271 332 430 1311
Size: 67 58 146 61 98 881
Fragments arranged by size:
881 146 98 67 61 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 702 734 981 1013 1311
Size: 702 32 247 32 298
Fragments arranged by size:
702 298 247 32 32
HincII GTy'rAC
Cuts at: 0 570 1311
Size: 570 741
KasI G'GCGC_C
Cuts at: 0 63 267 328 1311
Size: 63 204 61 983
Fragments arranged by size:
983 204 63 61
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 1311
Size: 214 1097
MslI CAynn'nnrTG
Cuts at: 0 346 595 1081 1311
Size: 346 249 486 230
Fragments arranged by size:
486 346 249 230
MspA1I CmG'CkG
Cuts at: 0 212 300 453 659 895 1311
Size: 212 88 153 206 236 416
Fragments arranged by size:
416 236 212 206 153 88
NaeI GCC'GGC
Cuts at: 0 126 1311
Size: 126 1185
NarI GG'CG_CC
Cuts at: 0 64 268 329 1311
Size: 64 204 61 982
Fragments arranged by size:
982 204 64 61
NgoMIV G'CCGG_C
Cuts at: 0 124 1311
Size: 124 1187
NspI r_CATG'y
Cuts at: 0 1080 1086 1311
Size: 1080 6 225
Fragments arranged by size:
1080 225 6
PasI CC'CwG_GG
Cuts at: 0 136 263 1311
Size: 136 127 1048
Fragments arranged by size:
1048 136 127
PciI A'CATG_T
Cuts at: 0 1082 1311
Size: 1082 229
PpuMI rG'GwC_Cy
Cuts at: 0 865 1311
Size: 865 446
PsiI TTA'TAA
Cuts at: 0 1217 1311
Size: 1217 94
PspOMI G'GGCC_C
Cuts at: 0 84 1311
Size: 84 1227
PstI C_TGCA'G
Cuts at: 0 727 894 1119 1311
Size: 727 167 225 192
Fragments arranged by size:
727 225 192 167
PvuII CAG'CTG
Cuts at: 0 659 895 1311
Size: 659 236 416
Fragments arranged by size:
659 416 236
SapI GCTCTTCn'nnn_
Cuts at: 0 34 1311
Size: 34 1277
SfcI C'TryA_G
Cuts at: 0 654 723 890 1115 1311
Size: 654 69 167 225 196
Fragments arranged by size:
654 225 196 167 69
SfoI GGC'GCC
Cuts at: 0 65 269 330 1311
Size: 65 204 61 981
Fragments arranged by size:
981 204 65 61
StyI C'CwwG_G
Cuts at: 0 699 1311
Size: 699 612
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 990 1311
Size: 990 321
TatI w'GTAC_w
Cuts at: 0 1030 1079 1311
Size: 1030 49 232
Fragments arranged by size:
1030 232 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 1311
Size: 229 32 1050
Fragments arranged by size:
1050 229 32
XbaI T'CTAG_A
Cuts at: 0 1185 1311
Size: 1185 126
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 1044 1311
Size: 354 690 267
Fragments arranged by size:
690 354 267
XmnI GAAnn'nnTTC
Cuts at: 0 319 382 1311
Size: 319 63 929
Fragments arranged by size:
929 319 63
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlfI ApaI AvaI AvrII
BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI
BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaHI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BsrBI BsrFI BsrGI
BssHII BstXI BstYI BtgI EaeI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI MmeI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PpuMI PsiI
PspOMI PstI PvuII SapI SfcI SfoI StyI TaqII
TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BclI BfrBI BglII BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrDI BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MscI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XhoI XmaI ZraI