################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KasI KspI MamI MboI MfeI MflI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI Sau3AI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 172 GGTACC G^GTACC AcyI 181 GACGCC G[GA]^CG[CT]C AocI 167 CCTCAGG CC^T[ACGT]AGG Asp718I 172 GGTACC G^GTACC AvaII 240 GGACC G^G[AT]CC BanI 172 GGTACC G^G[CT][GA]CC BbrPI 558 CACGTG CAC^GTG BcnI 540 CCGGG CC^[GC]GG BfaI 377 CTAG C^TAG BsaAI 558 CACGTG [CT]AC^GT[GA] BsaHI 181 GACGCC G[GA]^CG[CT]C BsgI 528 GTGCAG GTGCAG(16/14) Bsp1407I 2 TGTACA T^GTACA BsrGI 2 TGTACA T^GTACA BstAPI 22 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 167 CCTCAGG CC^T[ACGT]AGG CfoI 394 GCGC GCG^C EarI 129 CTCTTC CTCTTC(1/4) Eco81I 167 CCTCAGG CC^T[ACGT]AGG EcoNI 449 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 393 GGCGCT [GA]GCGC^[CT] HapII 540 CCGG C^CGG HhaI 394 GCGC GCG^C Hin1I 181 GACGCC G[GA]^CG[CT]C HinP1I 394 GCGC G^CGC HpaII 540 CCGG C^CGG KpnI 172 GGTACC GGTAC^C Ksp632I 129 CTCTTC CTCTTC(1/4) MaeI 377 CTAG C^TAG MaeII 559 ACGT A^CGT MboII 130 TCTTC GAAGA(8/7) MslI 570 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 540 CCGG C^CGG NciI 540 CCGGG CC^[GC]GG PmaCI 558 CACGTG CAC^GTG PmlI 558 CACGTG CAC^GTG PshAI 181 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 128 GCTCTTC GCTCTTC(1/4) SspBI 2 TGTACA T^GTACA StuI 164 AGGCCT AGG^CCT TaqI 39 TCGA T^CGA TthHB8I 39 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 3 GTAC GT^AC 173 GTAC AspHI 286 GTGCTC G[AT]GC[AT]^C 385 GAGCAC BbvCI 111 CCTCAGC CCTCAGC(-5/-2) 303 GCTGAGG BsiHKAI 286 GTGCTC G[AT]GC[AT]^C 385 GAGCAC BsmFI 315 GTCCC GGGAC(10/14) 630 GTCCC HgaI 181 GACGC GACGC(5/10) 590 GACGC HinfI 399 GAGTC G^A[ACGT]TC 413 GACTC NlaIII 43 CATG CATG^ 576 CATG PflMI 66 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 443 CCAGCTCCTGG PleI 413 GACTC GAGTC(4/5) 399 GAGTC RsaI 3 GTAC GT^AC 173 GTAC SbfI 19 CCTGCAGG CCTGCA^GG 228 CCTGCAGG Sse8387I 19 CCTGCAGG CCTGCA^GG 228 CCTGCAGG Van91I 66 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 443 CCAGCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AluI AlwNI ApaI BalI BanII BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII ItaI MaeIII MluNI MnlI MscI MspA1I MvaI MwoI NlaIV PstI PvuII Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI