(Linear) (Six-base) MAPSORT of: KIEE3925.seq Check: 4502 from: 1 to: 669 KIEE3925.orf With 182 enzymes: SgfI * March 22, 2012 16:52 .. Acc65I G'GTAC_C Cuts at: 0 172 669 Size: 172 497 AlwNI CAG_nnn'CTG Cuts at: 0 28 72 371 449 507 669 Size: 28 44 299 78 58 162 Fragments arranged by size: 299 162 78 58 44 28 ApaI G_GGCC'C Cuts at: 0 205 228 435 669 Size: 205 23 207 234 Fragments arranged by size: 234 207 205 23 BanI G'GyrC_C Cuts at: 0 172 669 Size: 172 497 BanII G_rGCy'C Cuts at: 0 93 205 228 435 669 Size: 93 112 23 207 234 Fragments arranged by size: 234 207 112 93 23 BbvCI CC'TCA_GC Cuts at: 0 112 304 669 Size: 112 192 365 Fragments arranged by size: 365 192 112 Bme1580I G_kGCm'C Cuts at: 0 205 228 435 565 669 Size: 205 23 207 130 104 Fragments arranged by size: 207 205 130 104 23 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 274 283 403 669 Size: 175 99 9 120 266 Fragments arranged by size: 266 175 120 99 9 Bpu10I CC'TnA_GC Cuts at: 0 112 304 319 669 Size: 112 192 15 350 Fragments arranged by size: 350 192 112 15 BsaAI yAC'GTr Cuts at: 0 560 669 Size: 560 109 BsaHI Gr'CG_yC Cuts at: 0 182 669 Size: 182 487 BseYI C'CCAG_C Cuts at: 0 98 428 602 669 Size: 98 330 174 67 Fragments arranged by size: 330 174 98 67 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 549 669 Size: 549 120 BsiHKAI G_wGCw'C Cuts at: 0 290 389 669 Size: 290 99 280 Fragments arranged by size: 290 280 99 Bsp1286I G_dGCh'C Cuts at: 0 93 205 228 290 389 435 565 669 Size: 93 112 23 62 99 46 130 104 Fragments arranged by size: 130 112 104 99 93 62 46 23 BsrGI T'GTAC_A Cuts at: 0 2 669 Size: 2 667 BstAPI GCAn_nnn'nTGC Cuts at: 0 28 669 Size: 28 641 Bsu36I CC'TnA_GG Cuts at: 0 168 669 Size: 168 501 BtsI GCAGTG_nn' Cuts at: 0 123 402 669 Size: 123 279 267 Fragments arranged by size: 279 267 123 EaeI y'GGCC_r Cuts at: 0 45 362 451 554 578 669 Size: 45 317 89 103 24 91 Fragments arranged by size: 317 103 91 89 45 24 EarI CTCTTCn'nnn_ Cuts at: 0 135 669 Size: 135 534 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 274 283 403 669 Size: 175 99 9 120 266 Fragments arranged by size: 266 175 120 99 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 453 669 Size: 453 216 EcoO109I rG'GnC_Cy Cuts at: 0 69 201 202 225 669 Size: 69 132 1 23 444 Fragments arranged by size: 444 132 69 23 1 HaeII r_GCGC'y Cuts at: 0 397 669 Size: 397 272 KpnI G_GTAC'C Cuts at: 0 176 669 Size: 176 493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 218 527 669 Size: 218 309 142 Fragments arranged by size: 309 218 142 MscI TGG'CCA Cuts at: 0 364 453 556 669 Size: 364 89 103 113 Fragments arranged by size: 364 113 103 89 MslI CAynn'nnrTG Cuts at: 0 574 669 Size: 574 95 MspA1I CmG'CkG Cuts at: 0 102 303 312 482 606 669 Size: 102 201 9 170 124 63 Fragments arranged by size: 201 170 124 102 63 9 PasI CC'CwG_GG Cuts at: 0 72 669 Size: 72 597 PflMI CCAn_nnn'nTGG Cuts at: 0 72 449 669 Size: 72 377 220 Fragments arranged by size: 377 220 72 PmlI CAC'GTG Cuts at: 0 560 669 Size: 560 109 PshAI GACnn'nnGTC Cuts at: 0 185 669 Size: 185 484 PspOMI G'GGCC_C Cuts at: 0 201 224 431 669 Size: 201 23 207 238 Fragments arranged by size: 238 207 201 23 PstI C_TGCA'G Cuts at: 0 24 33 233 256 409 464 487 669 Size: 24 9 200 23 153 55 23 182 Fragments arranged by size: 200 182 153 55 24 23 23 9 PvuII CAG'CTG Cuts at: 0 102 303 312 482 606 669 Size: 102 201 9 170 124 63 Fragments arranged by size: 201 170 124 102 63 9 SapI GCTCTTCn'nnn_ Cuts at: 0 135 669 Size: 135 534 SbfI CC_TGCA'GG Cuts at: 0 24 233 669 Size: 24 209 436 Fragments arranged by size: 436 209 24 SfcI C'TryA_G Cuts at: 0 20 29 229 252 405 460 483 669 Size: 20 9 200 23 153 55 23 186 Fragments arranged by size: 200 186 153 55 23 23 20 9 StuI AGG'CCT Cuts at: 0 166 669 Size: 166 503 StyI C'CwwG_G Cuts at: 0 176 281 454 669 Size: 176 105 173 215 Fragments arranged by size: 215 176 173 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2 669 Size: 2 667 TatI w'GTAC_w Cuts at: 0 2 669 Size: 2 667 Enzymes that do cut: Acc65I AlwNI ApaI BanI BanII BbvCI Bme1580I BpmI Bpu10I BsaAI BsaHI BseYI BsgI BsiHKAI Bsp1286I BsrGI BstAPI Bsu36I BtsI EaeI EarI Eco57MI EcoNI EcoO109I HaeII KpnI MmeI MscI MslI MspA1I PasI PflMI PmlI PshAI PspOMI PstI PvuII SapI SbfI SfcI StuI StyI TaqII TatI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI