################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsoBI BspDI BspHI BspLU11I BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeII MamI MflI MluI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccIII 643 TCCGGA T^CCGGA AcsI 309 AAATTT [GA]^AATT[CT] AflII 879 CTTAAG C^TTAAG ApoI 309 AAATTT [GA]^AATT[CT] AseI 429 ATTAAT AT^TAAT AsnI 429 ATTAAT AT^TAAT AspI 948 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 910 TGGCCA TGG^CCA BanII 187 GAGCTC G[GA]GC[CT]^C BcnI 794 CCGGG CC^[GC]GG BfaI 142 CTAG C^TAG BfrI 879 CTTAAG C^TTAAG BsaI 1036 GGTCTC GGTCTC(1/5) BsaWI 643 TCCGGA [AT]^CCGG[AT] BseAI 643 TCCGGA T^CCGGA BseRI 151 CTCCTC GAGGAG(10/8) BsmBI 246 CGTCTC CGTCTC(1/5) BspEI 643 TCCGGA T^CCGGA BsrBI 726 GAGCGG CCGCTC(-3/-3) BstEII 810 GGTCACC G^GT[ACGT]ACC BstPI 810 GGTCACC G^GT[ACGT]ACC DraII 652 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 1336 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 910 TGGCCA [CT]^GGCC[GA] EcoO109I 652 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 810 GGTCACC G^GT[ACGT]ACC EcoT14I 815 CCAAGG C^C[AT][AT]GG FauI 159 GCGGG CCCGC(4/6) HgaI 343 GACGC GACGC(5/10) MaeI 142 CTAG C^TAG MboII 357 GAAGA GAAGA(8/7) MfeI 914 CAATTG C^AATTG MluNI 910 TGGCCA TGG^CCA MroI 643 TCCGGA T^CCGGA MscI 910 TGGCCA TGG^CCA MspA1I 569 CAGCGG C[AC]G^C[GT]G MunI 914 CAATTG C^AATTG NciI 794 CCGGG CC^[GC]GG PflFI 948 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 652 AGGACCC [GA]G^G[AT]CC[CT] PshBI 429 ATTAAT AT^TAAT SacI 187 GAGCTC GAGCT^C SexAI 1337 ACCTGGT A^CC[AT]GGT SphI 1316 GCATGC GCATG^C StyI 815 CCAAGG C^C[AT][AT]GG TaqI 215 TCGA T^CGA TfiI 331 GATTC G^A[AT]TC Tth111I 948 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 215 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 217 GATCC GGATC(4/5) 87 GGATC AvaII 402 GGACC G^G[AT]CC 653 GGACC BsaJI 182 CCCTGG C^C[ACGT][ACGT]GG 815 CCAAGG BsgI 566 GTGCAG GTGCAG(16/14) 884 GTGCAG Cfr13I 402 GGACC G^G[ACGT]CC 653 GGACC DpnII 88 GATC ^GATC 217 GATC EcoRV 715 GATATC GAT^ATC 1126 GATATC HapII 644 CCGG C^CGG 794 CCGG HindIII 592 AAGCTT A^AGCTT 1239 AAGCTT HpaII 644 CCGG C^CGG 794 CCGG MboI 88 GATC ^GATC 217 GATC MspI 644 CCGG C^CGG 794 CCGG NdeII 88 GATC ^GATC 217 GATC PleI 147 GACTC GAGTC(4/5) 1086 GACTC PstI 734 CTGCAG CTGCA^G 982 CTGCAG Sau3AI 88 GATC ^GATC 217 GATC Sau96I 402 GGACC G^G[ACGT]CC 653 GGACC Tsp45I 598 GTGAC ^GT[GC]AC 811 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AfaI AluI AspHI BbvI BmyI BpmI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI Cac8I DdeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MnlI MseI MslI MvaI MwoI NlaIII NlaIV NspI RsaI ScrFI SfaNI SfcI SmlI SspBI Tru9I TseI Tsp509I TspRI