(Circular) (Six-base) MAPSORT of: pFN21AE3625.seq Check: 102 from: 1 to: 6067 pFN21AE3625 6067 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3410 1937 Size: 1473 4594 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4130 5514 5782 1632 Size: 2498 1384 268 1917 Fragments arranged by size: 2498 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2884 820 Size: 197 1867 4003 Fragments arranged by size: 4003 1867 197 AflIII A'CryG_T Cuts at: 1203 4966 5943 1203 Size: 3763 977 1327 Fragments arranged by size: 3763 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 2568 1166 Size: 1402 4665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 3810 5382 3810 Size: 1572 4495 ApaLI G'TGCA_C Cuts at: 4124 5280 4124 Size: 1156 4911 ApoI r'AATT_y Cuts at: 1493 2314 3376 3602 3754 5863 1493 Size: 821 1062 226 152 2109 1697 Fragments arranged by size: 2109 1697 1062 821 226 152 AseI AT'TA_AT Cuts at: 160 1032 2435 4621 160 Size: 872 1403 2186 1606 Fragments arranged by size: 2186 1606 1403 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 3392 1870 Size: 73 1449 4545 Fragments arranged by size: 4545 1449 73 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3388 4843 618 Size: 325 217 627 1601 1455 1842 Fragments arranged by size: 1842 1601 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2196 3386 1391 Size: 805 1190 4072 Fragments arranged by size: 4072 1190 805 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5096 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4258 4292 1903 Size: 34 2321 34 3678 Fragments arranged by size: 3678 2321 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4678 136 Size: 107 122 71 631 605 3006 1525 Fragments arranged by size: 3006 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6062 6062 Size: 6067 BlpI GC'TnA_GC Cuts at: 3484 3484 Size: 6067 Bme1580I G_kGCm'C Cuts at: 1790 2679 2805 3291 4128 5284 1790 Size: 889 126 486 837 1156 2573 Fragments arranged by size: 2573 1156 889 837 486 126 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3798 4756 442 Size: 416 53 83 399 24 2381 958 1753 Fragments arranged by size: 2381 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2423 2889 4727 1239 Size: 971 213 466 1838 2579 Fragments arranged by size: 2579 1838 971 466 213 Bpu10I CC'TnA_GC Cuts at: 2126 2126 Size: 6067 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2144 3226 4192 5057 5355 2144 Size: 1082 966 865 298 2856 Fragments arranged by size: 2856 1082 966 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3047 4730 882 Size: 617 1548 1683 2219 Fragments arranged by size: 2219 1683 1548 617 BsaAI yAC'GTr Cuts at: 493 1204 3858 493 Size: 711 2654 2702 Fragments arranged by size: 2702 2654 711 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3874 4256 4891 275 Size: 53 83 186 936 2341 382 635 1451 Fragments arranged by size: 2341 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2648 4500 4898 5172 5319 5730 2648 Size: 1852 398 274 147 411 2985 Fragments arranged by size: 2985 1852 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5842 Fragments arranged by size: 5842 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2147 1186 Size: 961 5106 BseYI C'CCAG_C Cuts at: 2634 2661 2774 5270 5766 2634 Size: 27 113 2496 496 2935 Fragments arranged by size: 2935 2496 496 113 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2592 2910 1163 Size: 573 856 318 4320 Fragments arranged by size: 4320 856 573 318 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2196 2534 3363 4128 4213 5284 2196 Size: 338 829 765 85 1071 2979 Fragments arranged by size: 2979 1071 829 765 338 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 BsmBI CGTCTCn'nnnn_ Cuts at: 2257 2257 Size: 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2534 2679 2805 3291 3363 3386 Size: 399 406 338 145 126 486 72 23 Cuts at: 3386 4128 4213 5284 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 486 406 399 338 145 126 85 72 23 BspEI T'CCGG_A Cuts at: 2648 5730 2648 Size: 3082 2985 BspHI T'CATG_A Cuts at: 3956 5686 3956 Size: 1730 4337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3422 844 Size: 2578 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2733 3954 1394 Size: 429 910 1221 3507 Fragments arranged by size: 3507 1221 910 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4711 4898 1700 Size: 110 2901 187 2869 Fragments arranged by size: 2901 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2285 2489 3165 96 Size: 2189 204 676 2998 Fragments arranged by size: 2998 2189 676 204 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6067 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 2815 1184 Size: 1631 4436 BstYI r'GATC_y Cuts at: 1972 3397 3764 4149 4166 5618 5704 5716 6062 Size: 1425 367 385 17 1452 86 12 346 Cuts at: 6062 1972 Size: 1977 Fragments arranged by size: 1977 1452 1425 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4576 Fragments arranged by size: 4576 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 2546 3583 4377 4397 2546 Size: 1037 794 20 4216 Fragments arranged by size: 4216 1037 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DraIII CAC_nnn'GTG Cuts at: 3346 3346 Size: 6067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2915 3514 4403 Size: 54 1425 50 275 18 1085 599 889 Cuts at: 4403 6032 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1085 889 599 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3997 1353 Size: 2644 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4654 5028 5174 387 Size: 1064 138 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2194 2194 Size: 6067 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2423 2889 4130 4727 5514 5782 Size: 393 578 213 466 1241 597 787 268 Cuts at: 5782 1239 Size: 1524 Fragments arranged by size: 1524 1241 787 597 578 466 393 268 213 EcoO109I rG'GnC_Cy Cuts at: 2658 2658 Size: 6067 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 EcoRV GAT'ATC Cuts at: 2722 3133 2722 Size: 411 5656 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2185 2498 2872 2185 Size: 313 374 5380 Fragments arranged by size: 5380 374 313 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 5214 1678 Size: 3536 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2492 2524 2862 2894 4708 Size: 32 135 32 920 32 338 32 1814 Cuts at: 4708 4740 4782 4814 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1814 920 338 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3411 3657 669 Size: 988 281 1473 246 3079 Fragments arranged by size: 3079 1473 988 281 246 HindIII A'AGCT_T Cuts at: 748 2597 3244 748 Size: 1849 647 3571 Fragments arranged by size: 3571 1849 647 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 2919 3666 2919 Size: 747 5320 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5181 5365 1305 Size: 3876 184 2007 Fragments arranged by size: 3876 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2917 10 Size: 54 1425 343 1085 3160 Fragments arranged by size: 3160 1425 1085 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2568 3006 3320 4025 4384 4543 518 Size: 648 1402 438 314 705 359 159 2042 Fragments arranged by size: 2042 1402 705 648 438 359 314 159 MspA1I CmG'CkG Cuts at: 1606 2576 3483 4160 5308 5553 1606 Size: 970 907 677 1148 245 2120 Fragments arranged by size: 2120 1148 970 907 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4576 Fragments arranged by size: 4576 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 2244 2310 2784 3325 3425 4970 1132 Size: 97 1015 66 474 541 100 1545 2229 Fragments arranged by size: 2229 1545 1015 541 474 100 97 66 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6067 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6067 PfoI T'CCnGG_A Cuts at: 2866 2866 Size: 6067 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2658 2658 Size: 6067 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PstI C_TGCA'G Cuts at: 830 2743 2991 3419 830 Size: 1913 248 428 3478 Fragments arranged by size: 3478 1913 428 248 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 SacI G_AGCT'C Cuts at: 2196 2196 Size: 6067 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SexAI A'CCwGG_T Cuts at: 3342 3342 Size: 6067 SfcI C'TryA_G Cuts at: 826 1045 2739 2987 3025 3415 4550 5231 5422 Size: 219 1694 248 38 390 1135 681 191 Cuts at: 5422 826 Size: 1471 Fragments arranged by size: 1694 1471 1135 681 390 248 219 191 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 2884 3241 4171 5072 5334 Size: 197 926 216 725 357 930 901 262 Cuts at: 5334 820 Size: 1553 Fragments arranged by size: 1553 930 926 901 725 357 262 216 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3325 3425 1132 Size: 2193 100 3774 Fragments arranged by size: 3774 2193 100 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6067 StyI C'CwwG_G Cuts at: 513 1065 2004 2820 3506 513 Size: 552 939 816 686 3074 Fragments arranged by size: 3074 939 816 686 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4094 4111 4264 4449 4894 325 Size: 1255 2514 17 153 185 445 1498 Fragments arranged by size: 2514 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2285 2489 3165 Size: 274 80 33 51 1443 308 204 676 Cuts at: 3165 3219 4313 6025 96 Size: 54 1094 1712 138 Fragments arranged by size: 1712 1443 1094 676 308 274 204 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6027 6059 6027 Size: 32 6035 Tth111I GACn'n_nGTC Cuts at: 1316 2956 1316 Size: 1640 4427 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6067 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 4196 4196 Size: 6067 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI