Restriction map for
pFN21AE3625
(Linear) (Six-base) MAPSORT of: KIEE3625.seq Check: 3239 from: 1 to: 1362
KIEE3625.orf
With 182 enzymes: SgfI *
December 27, 2011 16:36 ..
AflII C'TTAA_G
Cuts at: 0 879 1362
Size: 879 483
AleI CACnn'nnGTG
Cuts at: 0 563 1362
Size: 563 799
ApoI r'AATT_y
Cuts at: 0 309 1362
Size: 309 1053
AseI AT'TA_AT
Cuts at: 0 430 1362
Size: 430 932
BanII G_rGCy'C
Cuts at: 0 191 1362
Size: 191 1171
Bme1580I G_kGCm'C
Cuts at: 0 674 800 1286 1362
Size: 674 126 486 76
Fragments arranged by size:
674 486 126 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 418 884 1362
Size: 205 213 466 478
Fragments arranged by size:
478 466 213 205
Bpu10I CC'TnA_GC
Cuts at: 0 121 1362
Size: 121 1241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1221 1362
Size: 139 1082 141
Fragments arranged by size:
1082 141 139
BsaI GGTCTCn'nnnn_
Cuts at: 0 1042 1362
Size: 1042 320
BsaWI w'CCGG_w
Cuts at: 0 643 1362
Size: 643 719
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 1362
Size: 142 1220
BseYI C'CCAG_C
Cuts at: 0 629 656 769 1362
Size: 629 27 113 593
Fragments arranged by size:
629 593 113 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 905 1362
Size: 587 318 457
Fragments arranged by size:
587 457 318
BsiHKAI G_wGCw'C
Cuts at: 0 191 529 1358 1362
Size: 191 338 829 4
Fragments arranged by size:
829 338 191 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 252 1362
Size: 252 1110
Bsp1286I G_dGCh'C
Cuts at: 0 191 529 674 800 1286 1358 1362
Size: 191 338 145 126 486 72 4
Fragments arranged by size:
486 338 191 145 126 72 4
BspEI T'CCGG_A
Cuts at: 0 643 1362
Size: 643 719
BsrBI CCG'CTC
Cuts at: 0 728 1362
Size: 728 634
BsrGI T'GTAC_A
Cuts at: 0 280 484 1160 1362
Size: 280 204 676 202
Fragments arranged by size:
676 280 204 202
BstEII G'GTnAC_C
Cuts at: 0 810 1362
Size: 810 552
BtsI GCAGTG_nn'
Cuts at: 0 541 1362
Size: 541 821
DraIII CAC_nnn'GTG
Cuts at: 0 1341 1362
Size: 1341 21
EaeI y'GGCC_r
Cuts at: 0 910 1362
Size: 910 452
EcoICRI GAG'CTC
Cuts at: 0 189 1362
Size: 189 1173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 418 884 1362
Size: 205 213 466 478
Fragments arranged by size:
478 466 213 205
EcoO109I rG'GnC_Cy
Cuts at: 0 653 1362
Size: 653 709
EcoRV GAT'ATC
Cuts at: 0 717 1128 1362
Size: 717 411 234
Fragments arranged by size:
717 411 234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 180 493 867 1362
Size: 180 313 374 495
Fragments arranged by size:
495 374 313 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 487 519 857 889 1362
Size: 487 32 338 32 473
Fragments arranged by size:
487 473 338 32 32
HindIII A'AGCT_T
Cuts at: 0 592 1239 1362
Size: 592 647 123
Fragments arranged by size:
647 592 123
MfeI C'AATT_G
Cuts at: 0 914 1362
Size: 914 448
MscI TGG'CCA
Cuts at: 0 912 1362
Size: 912 450
MslI CAynn'nnrTG
Cuts at: 0 563 1001 1315 1362
Size: 563 438 314 47
Fragments arranged by size:
563 438 314 47
MspA1I CmG'CkG
Cuts at: 0 571 1362
Size: 571 791
NspI r_CATG'y
Cuts at: 0 239 305 779 1320 1362
Size: 239 66 474 541 42
Fragments arranged by size:
541 474 239 66 42
PfoI T'CCnGG_A
Cuts at: 0 861 1362
Size: 861 501
PpuMI rG'GwC_Cy
Cuts at: 0 653 1362
Size: 653 709
PstI C_TGCA'G
Cuts at: 0 738 986 1362
Size: 738 248 376
Fragments arranged by size:
738 376 248
SacI G_AGCT'C
Cuts at: 0 191 1362
Size: 191 1171
SexAI A'CCwGG_T
Cuts at: 0 1337 1362
Size: 1337 25
SfcI C'TryA_G
Cuts at: 0 734 982 1020 1362
Size: 734 248 38 342
Fragments arranged by size:
734 342 248 38
SmlI C'TyrA_G
Cuts at: 0 154 879 1236 1362
Size: 154 725 357 126
Fragments arranged by size:
725 357 154 126
SphI G_CATG'C
Cuts at: 0 1320 1362
Size: 1320 42
StyI C'CwwG_G
Cuts at: 0 815 1362
Size: 815 547
TatI w'GTAC_w
Cuts at: 0 280 484 1160 1214 1362
Size: 280 204 676 54 148
Fragments arranged by size:
676 280 204 148 54
Tth111I GACn'n_nGTC
Cuts at: 0 951 1362
Size: 951 411
Enzymes that do cut:
AflII AleI ApoI AseI BanII Bme1580I BpmI Bpu10I
BpuEI BsaI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspEI BsrBI BsrGI BstEII BtsI DraIII EaeI
EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I HindIII MfeI
MscI MslI MspA1I NspI PfoI PpuMI PstI SacI
SexAI SfcI SmlI SphI StyI TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI BsaXI
BsiEI BsiWI BsmI BspHI BspMI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DrdI EagI EarI EciI
EcoNI EcoRI FseI FspI FspAI HaeII HincII HpaI
KasI KpnI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TstI XbaI
XcmI XhoI XmaI XmnI ZraI