################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BsiWI BsmBI BsmI BspDI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1408 GTCTAC GT^[AC][GT]AC AccIII 642 TCCGGA T^CCGGA Alw44I 1378 GTGCAC G^TGCAC AocI 659 CCTCAGG CC^T[ACGT]AGG ApaLI 1378 GTGCAC G^TGCAC Asp700I 1356 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 59 GGCGCC GGCGC^C BbrPI 114 CACGTG CAC^GTG BbsI 1058 GAAGAC GAAGAC(2/6) BbvCI 1549 GCTGAGG CCTCAGC(-5/-2) BcgI 83 CGAGCACCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 618 GCTCAGC GC^T[ACGT]AGC Bpu1102I 618 GCTCAGC GC^T[ACGT]AGC BpuAI 1058 GAAGAC GAAGAC(2/6) BsaAI 114 CACGTG [CT]AC^GT[GA] BsaWI 642 TCCGGA [AT]^CCGG[AT] BseAI 642 TCCGGA T^CCGGA BsgI 1526 CTGCAC GTGCAG(16/14) Bsp1407I 416 TGTACA T^GTACA BspEI 642 TCCGGA T^CCGGA BspHI 512 TCATGA T^CATGA BspLU11I 1478 ACATGT A^CATGT BsrDI 1508 CATTGC GCAATG(2/0) BsrGI 416 TGTACA T^GTACA BstEII 1113 GGTGACC G^GT[ACGT]ACC BstPI 1113 GGTGACC G^GT[ACGT]ACC Bsu36I 659 CCTCAGG CC^T[ACGT]AGG CelII 618 GCTCAGC GC^T[ACGT]AGC DraIII 64 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 659 CCTCAGG CC^T[ACGT]AGG EcoO65I 1113 GGTGACC G^GT[ACGT]ACC KasI 59 GGCGCC G^GCGCC MroI 642 TCCGGA T^CCGGA NarI 59 GGCGCC GG^CGCC PaeR7I 303 CTCGAG C^TCGAG PleI 316 GACTC GAGTC(4/5) PmaCI 114 CACGTG CAC^GTG PmlI 114 CACGTG CAC^GTG RcaI 512 TCATGA T^CATGA SacI 616 GAGCTC GAGCT^C SfaNI 278 GCATC GCATC(5/9) SfiI 770 GGCCCACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 59 GGCGCC GGC^GCC SphI 1434 GCATGC GCATG^C SspBI 416 TGTACA T^GTACA TfiI 607 GAATC G^A[AT]TC XhoI 303 CTCGAG C^TCGAG XmnI 1356 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 59 GGCGCC G[GA]^CG[CT]C 78 GACGCC AfaI 417 GTAC GT^AC 1476 GTAC AflIII 113 ACACGT A^C[GA][CT]GT 1478 ACATGT Aor51HI 589 AGCGCT AGC^GCT 1157 AGCGCT BamHI 867 GGATCC G^GATCC 903 GGATCC BsaHI 59 GGCGCC G[GA]^CG[CT]C 78 GACGCC BspMI 206 ACCTGC ACCTGC(4/8) 1402 GCAGGT BsrI 189 CCAGT ACTGG(1/-1) 406 CCAGT BssSI 420 CACGAG CACGAG(-5/-1) 774 CACGAG BstF5I 1581 CATCC GGATG(2/0) 426 GGATG BstYI 867 GGATCC [GA]^GATC[CT] 903 GGATCC DrdI 462 GACGCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1195 GACCGCTACGTC EarI 1360 CTCTTC CTCTTC(1/4) 1605 GAAGAG Eco47III 589 AGCGCT AGC^GCT 1157 AGCGCT EcoNI 1344 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1614 CCTGGGCAAGG FokI 1581 CATCC GGATG(9/13) 426 GGATG Hin1I 59 GGCGCC G[GA]^CG[CT]C 78 GACGCC HinfI 316 GACTC G^A[ACGT]TC 607 GAATC Ksp632I 1360 CTCTTC CTCTTC(1/4) 1605 GAAGAG KspI 29 CCGCGG CCGC^GG 624 CCGCGG MflI 867 GGATCC [GA]^GATC[CT] 903 GGATCC NspI 1434 GCATGC [GA]CATG^[CT] 1478 ACATGT PpuMI 1181 GGGTCCT [GA]G^G[AT]CC[CT] 1495 GGGTCCC RsaI 417 GTAC GT^AC 1476 GTAC SacII 29 CCGCGG CCGC^GG 624 CCGCGG TaqI 176 TCGA T^CGA 304 TCGA TspRI 190 CAGTG CA[GC]TG[ACGT][ACGT]^ 1235 CAGTG TthHB8I 176 TCGA T^CGA 304 TCGA XcmI 221 CCAAGCTGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1226 CCAGCGAGACAGTGG XhoII 867 GGATCC [GA]^GATC[CT] 903 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PstI PvuII Sau3AI Sau96I ScrFI SfcI SmlI StuI StyI TseI Tsp45I