Restriction map for
pFN21AB6819
##################################
# NO CUT ENZYMES ( 192 enzymes) #
##################################
AatII Acc65I AccI AccII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BcnI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspDI BspLU11I BspMI BsrBI
BsrFI BssHII BssKI BssSI Bst1107I
BstAPI BstEII BstNI BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DdeI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT14I
FauI Fnu4HI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI ItaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MscI
MspA1I MunI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI ScrFI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI StyI SwaI TseI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccIII 304 TCCGGA T^CCGGA
AfaI 454 GTAC GT^AC
AluI 84 AGCT AG^CT
AlwNI 386 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 26 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 341 TGATCA T^GATCA
BfaI 349 CTAG C^TAG
BglII 183 AGATCT A^GATCT
BpmI 415 CTGGAG CTGGAG(16/14)
BsaWI 304 TCCGGA [AT]^CCGG[AT]
BseAI 304 TCCGGA T^CCGGA
BsgI 119 GTGCAG GTGCAG(16/14)
BsmI 89 GCATTC GAATGC(1/-1)
Bsp1407I 453 TGTACA T^GTACA
BspEI 304 TCCGGA T^CCGGA
BspHI 321 TCATGA T^CATGA
BsrDI 259 GCAATG GCAATG(2/0)
BsrGI 453 TGTACA T^GTACA
BstBI 9 TTCGAA TT^CGAA
BstYI 183 AGATCT [GA]^GATC[CT]
Cac8I 85 GCTGGC GC[ACGT]^[ACGT]GC
EcoT22I 199 ATGCAT ATGCA^T
FbaI 341 TGATCA T^GATCA
HapII 305 CCGG C^CGG
HpaII 305 CCGG C^CGG
MaeI 349 CTAG C^TAG
MflI 183 AGATCT [GA]^GATC[CT]
MroI 304 TCCGGA T^CCGGA
MseI 431 TTAA T^TAA
MslI 202 CATATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 305 CCGG C^CGG
NsiI 199 ATGCAT ATGCA^T
NspV 9 TTCGAA TT^CGAA
PleI 301 GAGTC GAGTC(4/5)
RcaI 321 TCATGA T^CATGA
RsaI 454 GTAC GT^AC
SfuI 9 TTCGAA TT^CGAA
SmlI 298 CTTGAG C^T[CT][GA]AG
SspBI 453 TGTACA T^GTACA
SspI 427 AATATT AAT^ATT
TaqI 10 TCGA T^CGA
TfiI 388 GAATC G^A[AT]TC
Tru9I 431 TTAA T^TAA
TthHB8I 10 TCGA T^CGA
XhoII 183 AGATCT [GA]^GATC[CT]
XmnI 26 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 5 GAATTT [GA]^AATT[CT]
436 GAATTT
ApoI 5 GAATTT [GA]^AATT[CT]
436 GAATTT
BbsI 97 GTCTTC GAAGAC(2/6)
366 GTCTTC
BpuAI 97 GTCTTC GAAGAC(2/6)
366 GTCTTC
DpnII 184 GATC ^GATC
342 GATC
HinfI 301 GAGTC G^A[ACGT]TC
388 GAATC
HphI 150 GGTGA GGTGA(8/7)
237 TCACC
MboI 184 GATC ^GATC
342 GATC
MnlI 409 GAGG CCTC(7/6)
418 GAGG
NdeII 184 GATC ^GATC
342 GATC
NlaIII 94 CATG CATG^
322 CATG
Sau3AI 184 GATC ^GATC
342 GATC
TspRI 40 CAGTG CA[GC]TG[ACGT][ACGT]^
117 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
BsrI BstF5I FokI MaeIII MboII
SfaNI Tsp509I