################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BciVI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SapI SbfI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 1240 GGTACC G^GTACC AccI 1028 GTCGAC GT^[AC][GT]AC AcsI 760 GAATTC [GA]^AATT[CT] Alw44I 700 GTGCAC G^TGCAC ApaLI 700 GTGCAC G^TGCAC ApoI 760 GAATTC [GA]^AATT[CT] Asp700I 57 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1240 GGTACC G^GTACC AspHI 700 GTGCAC G[AT]GC[AT]^C AvaI 1229 CTCGGG C^[CT]CG[GA]G BanI 1240 GGTACC G^G[CT][GA]CC BanII 739 GGGCTC G[GA]GC[CT]^C BbvCI 1089 GCTGAGG CCTCAGC(-5/-2) BglI 263 GCCCATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 890 AGATCT A^GATCT BsaAI 1106 TACGTA [CT]AC^GT[GA] BsaWI 458 ACCGGA [AT]^CCGG[AT] BsiHKAI 700 GTGCAC G[AT]GC[AT]^C BsmBI 656 GAGACG CGTCTC(1/5) BsoBI 1229 CTCGGG C^[CT]CG[GA]G BspHI 764 TCATGA T^CATGA BsrI 195 CCAGT ACTGG(1/-1) BstAPI 1332 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 597 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 804 CCTGGCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 760 GAATTC G^AATTC HgaI 1137 GACGC GACGC(5/10) KpnI 1240 GGTACC GGTAC^C MaeII 1107 ACGT A^CGT MslI 555 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 781 CAGCGG C[AC]G^C[GT]G PflMI 571 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1121 AGGACCT [GA]G^G[AT]CC[CT] RcaI 764 TCATGA T^CATGA SalI 1028 GTCGAC G^TCGAC ScaI 215 AGTACT AGT^ACT SexAI 570 ACCAGGT A^CC[AT]GGT SfcI 995 CTGTAG C^T[GA][CT]AG SnaBI 1106 TACGTA TAC^GTA SphI 513 GCATGC GCATG^C TaqI 1029 TCGA T^CGA TthHB8I 1029 TCGA T^CGA Van91I 571 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 57 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccII 188 CGCG CG^CG 1139 CGCG AlwNI 1120 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 1283 CAGAAACTG BclI 2 TGATCA T^GATCA 630 TGATCA BpmI 16 CTGGAG CTGGAG(16/14) 169 CTGGAG BseRI 865 GAGGAG GAGGAG(10/8) 1315 GAGGAG BsmAI 656 GAGAC GTCTC(1/5) 1144 GAGAC BstUI 188 CGCG CG^CG 1139 CGCG BstYI 890 AGATCT [GA]^GATC[CT] 1278 AGATCC EaeI 732 CGGCCA [CT]^GGCC[GA] 745 CGGCCA FbaI 2 TGATCA T^GATCA 630 TGATCA HindIII 274 AAGCTT A^AGCTT 1294 AAGCTT MaeIII 911 GTGAC ^GT[ACGT]AC 1135 GTGAC MflI 890 AGATCT [GA]^GATC[CT] 1278 AGATCC MvnI 188 CGCG CG^CG 1139 CGCG NcoI 265 CCATGG C^CATGG 735 CCATGG NspI 513 GCATGC [GA]CATG^[CT] 1115 ACATGC Tsp45I 911 GTGAC ^GT[GC]AC 1135 GTGAC XhoII 890 AGATCT [GA]^GATC[CT] 1278 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI TseI Tsp509I TspRI