################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BbrPI BfrI BglI BglII BlnI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI MfeI MluI MroI MunI NaeI NcoI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcsI 615 GAATTT [GA]^AATT[CT] AfaI 1308 GTAC GT^AC AflIII 812 ACGTGT A^C[GA][CT]GT AhdI 66 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 615 GAATTT [GA]^AATT[CT] Asp700I 531 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 66 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 776 GGATCC G^GATCC BbsI 1450 GAAGAC GAAGAC(2/6) BbvCI 387 CCTCAGC CCTCAGC(-5/-2) BcgI 1403 CGATTACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 266 TGATCA T^GATCA BcnI 996 CCGGG CC^[GC]GG BfaI 1171 CTAG C^TAG BlpI 165 GCTCAGC GC^T[ACGT]AGC BpmI 1049 CTCCAG CTGGAG(16/14) Bpu1102I 165 GCTCAGC GC^T[ACGT]AGC BpuAI 1450 GAAGAC GAAGAC(2/6) BsaAI 985 TACGTG [CT]AC^GT[GA] BsaBI 1089 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1443 ACCGGA [AT]^CCGG[AT] BseRI 437 CTCCTC GAGGAG(10/8) BsmAI 74 GTCTC GTCTC(1/5) BspHI 806 TCATGA T^CATGA BstEII 880 GGTGACC G^GT[ACGT]ACC BstPI 880 GGTGACC G^GT[ACGT]ACC CelII 165 GCTCAGC GC^T[ACGT]AGC DsaI 542 CCGCGG C^C[GA][CT]GG Eam1105I 66 GACCACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 331 CTCTTC CTCTTC(1/4) EcoNI 297 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 880 GGTGACC G^GT[ACGT]ACC EcoT14I 600 CCAAGG C^C[AT][AT]GG FbaI 266 TGATCA T^GATCA Ksp632I 331 CTCTTC CTCTTC(1/4) KspI 542 CCGCGG CCGC^GG MaeI 1171 CTAG C^TAG MamI 1089 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NciI 996 CCGGG CC^[GC]GG NdeI 201 CATATG CA^TATG NruI 224 TCGCGA TCG^CGA NspI 1007 ACATGC [GA]CATG^[CT] PflMI 858 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 850 CTGCAG CTGCA^G PvuII 643 CAGCTG CAG^CTG RcaI 806 TCATGA T^CATGA RsaI 1308 GTAC GT^AC SacII 542 CCGCGG CCGC^GG SfcI 850 CTGCAG C^T[GA][CT]AG StyI 600 CCAAGG C^C[AT][AT]GG Van91I 858 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1365 CCACTACAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 531 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 225 CGCG CG^CG 543 CGCG AcyI 637 GGCGCC G[GA]^CG[CT]C 1040 GGCGCC ApaI 187 GGGCCC GGGCC^C 313 GGGCCC AspHI 163 GTGCTC G[AT]GC[AT]^C 589 GTGCTC BalI 563 TGGCCA TGG^CCA 761 TGGCCA BbeI 637 GGCGCC GGCGC^C 1040 GGCGCC BbvI 428 GCTGC GCAGC(8/12) 946 GCTGC BciVI 6 GGATAC GTATCC(6/5) 288 GTATCC BsaHI 637 GGCGCC G[GA]^CG[CT]C 1040 GGCGCC BsiHKAI 163 GTGCTC G[AT]GC[AT]^C 589 GTGCTC BspMI 752 ACCTGC ACCTGC(4/8) 958 ACCTGC BsrI 10 ACTGG ACTGG(1/-1) 71 CCAGT BstUI 225 CGCG CG^CG 543 CGCG BstXI 110 CCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 659 CCATCAGCCTGG BstYI 140 GGATCT [GA]^GATC[CT] 776 GGATCC HaeII 637 GGCGCC [GA]GCGC^[CT] 1040 GGCGCC HapII 996 CCGG C^CGG 1444 CCGG HgaI 228 GACGC GACGC(5/10) 1034 GCGTC Hin1I 637 GGCGCC G[GA]^CG[CT]C 1040 GGCGCC HpaII 996 CCGG C^CGG 1444 CCGG KasI 637 GGCGCC G^GCGCC 1040 GGCGCC MflI 140 GGATCT [GA]^GATC[CT] 776 GGATCC MluNI 563 TGGCCA TGG^CCA 761 TGGCCA MscI 563 TGGCCA TGG^CCA 761 TGGCCA MseI 359 TTAA T^TAA 1333 TTAA MspI 996 CCGG C^CGG 1444 CCGG MvnI 225 CGCG CG^CG 543 CGCG NarI 637 GGCGCC GG^CGCC 1040 GGCGCC PpuMI 218 GGGTCCT [GA]G^G[AT]CC[CT] 955 GGGACCT SfaNI 1422 GATGC GCATC(5/9) 104 GCATC SfoI 637 GGCGCC GGC^GCC 1040 GGCGCC StuI 305 AGGCCT AGG^CCT 1213 AGGCCT TfiI 705 GAATC G^A[AT]TC 1167 GATTC Tru9I 359 TTAA T^TAA 1333 TTAA TseI 428 GCTGC G^C[AT]GC 946 GCTGC XhoII 140 GGATCT [GA]^GATC[CT] 776 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AluI AlwI AvaII BanI BanII BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TaqI Tsp45I Tsp509I TspRI TthHB8I