(Circular) (Six-base) MAPSORT of: pFN21AE4465.seq Check: 6630 from: 1 to: 6166 pFN21AE4465 6166 bp With 182 enzymes: SgfI * March 28, 2012 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3976 278 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3509 1937 Size: 1572 4594 Acc65I G'GTAC_C Cuts at: 3487 3487 Size: 6166 AclI AA'CG_TT Cuts at: 1563 4293 4666 1563 Size: 2730 373 3063 Fragments arranged by size: 3063 2730 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3407 4229 5613 5881 1632 Size: 1775 822 1384 268 1917 Fragments arranged by size: 1917 1775 1384 822 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5969 AflIII A'CryG_T Cuts at: 1203 2817 5065 6042 1203 Size: 1614 2248 977 1327 Fragments arranged by size: 2248 1614 1327 977 AgeI A'CCGG_T Cuts at: 4997 4997 Size: 6166 AhdI GACnn_n'nnGTC Cuts at: 2076 4895 2076 Size: 2819 3347 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6166 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6166 AlwNI CAG_nnn'CTG Cuts at: 3909 5481 3909 Size: 1572 4594 ApaI G_GGCC'C Cuts at: 2196 2322 2196 Size: 126 6040 ApaLI G'TGCA_C Cuts at: 4223 5379 4223 Size: 1156 5010 ApoI r'AATT_y Cuts at: 1493 2620 3475 3701 3853 5962 1493 Size: 1127 855 226 152 2109 1697 Fragments arranged by size: 2109 1697 1127 855 226 152 AseI AT'TA_AT Cuts at: 160 1032 4720 160 Size: 872 3688 1606 Fragments arranged by size: 3688 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 AvaI C'yCGr_G Cuts at: 1870 1943 3491 1870 Size: 73 1548 4545 Fragments arranged by size: 4545 1548 73 BamHI G'GATC_C Cuts at: 2781 3496 3863 2781 Size: 715 367 5084 Fragments arranged by size: 5084 715 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2126 2240 2642 3045 3229 Size: 325 217 627 339 114 402 403 184 Cuts at: 3229 3487 4942 618 Size: 258 1455 1842 Fragments arranged by size: 1842 1455 627 403 402 339 325 258 217 184 114 BanII G_rGCy'C Cuts at: 1391 2196 2322 3391 3485 1391 Size: 805 126 1069 94 4072 Fragments arranged by size: 4072 1069 805 126 94 BbeI G_GCGC'C Cuts at: 2646 3049 2646 Size: 403 5763 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3462 928 Size: 971 1563 3632 Fragments arranged by size: 3632 1563 971 BbvCI CC'TCA_GC Cuts at: 2393 2393 Size: 6166 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3397 3431 4357 4391 1903 Size: 34 1460 34 926 34 3678 Fragments arranged by size: 3678 1460 926 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2005 2304 4058 5268 2005 Size: 299 1754 1210 2903 Fragments arranged by size: 2903 1754 1210 299 BclI T'GATC_A Cuts at: 1548 2271 1548 Size: 723 5443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4777 136 Size: 107 122 71 631 605 3105 1525 Fragments arranged by size: 3105 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6161 6161 Size: 6166 BlpI GC'TnA_GC Cuts at: 2171 3583 2171 Size: 1412 4754 Bme1580I G_kGCm'C Cuts at: 1790 2129 2196 2245 2322 3234 4227 5383 1790 Size: 339 67 49 77 912 993 1156 2573 Fragments arranged by size: 2573 1156 993 912 339 77 67 49 BmgBI CAC'GTC Cuts at: 1318 2373 1318 Size: 1055 5111 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3897 4855 442 Size: 416 53 83 399 24 2480 958 1753 Fragments arranged by size: 2480 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6166 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3039 4826 1239 Size: 1800 1787 2579 Fragments arranged by size: 2579 1800 1787 Bpu10I CC'TnA_GC Cuts at: 2393 2393 Size: 6166 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4291 5156 5454 4291 Size: 865 298 5003 Fragments arranged by size: 5003 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4829 882 Size: 617 3330 2219 Fragments arranged by size: 3330 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2992 3957 493 Size: 711 1788 965 2702 Fragments arranged by size: 2702 1788 965 711 BsaBI GATnn'nnATC Cuts at: 3098 6160 3098 Size: 3062 3104 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2643 3046 3973 4355 Size: 53 83 186 936 1110 403 927 382 Cuts at: 4355 4990 275 Size: 635 1451 Fragments arranged by size: 1451 1110 936 927 635 403 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3448 4599 4997 5271 5418 5829 3448 Size: 1151 398 274 147 411 3785 Fragments arranged by size: 3785 1151 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2324 2354 1178 Size: 30 165 30 921 30 4990 Fragments arranged by size: 4990 921 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2433 1186 Size: 1247 4919 BseYI C'CCAG_C Cuts at: 2092 2236 2518 2646 5369 5865 2092 Size: 144 282 128 2723 496 2393 Fragments arranged by size: 2723 2393 496 282 144 128 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5593 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3616 4377 5405 1540 Size: 275 186 1615 761 1028 2301 Fragments arranged by size: 2301 1615 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2598 4227 4312 5383 2172 Size: 426 1629 85 1071 2955 Fragments arranged by size: 2955 1629 1071 426 85 BsmI GAATG_Cn' Cuts at: 3677 3770 3677 Size: 93 6073 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2172 2196 2245 2322 2598 3234 Size: 399 339 43 24 49 77 276 636 Cuts at: 3234 3391 3485 4227 4312 5383 1391 Size: 157 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 636 399 339 276 157 94 85 77 49 43 24 BspEI T'CCGG_A Cuts at: 5829 5829 Size: 6166 BspHI T'CATG_A Cuts at: 2811 4055 5785 2811 Size: 1244 1730 3192 Fragments arranged by size: 3192 1730 1244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2766 2972 3521 844 Size: 1922 206 549 3489 Fragments arranged by size: 3489 1922 549 206 BsrBI CCG'CTC Cuts at: 1394 1823 4053 1394 Size: 429 2230 3507 Fragments arranged by size: 3507 2230 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4661 4835 65 Size: 1183 3413 174 1396 Fragments arranged by size: 3413 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4810 4997 1700 Size: 110 3000 187 2869 Fragments arranged by size: 3000 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6166 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6166 BssSI C'ACGA_G Cuts at: 4226 5238 4226 Size: 1012 5154 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6166 BstBI TT'CG_AA Cuts at: 4970 4970 Size: 6166 BstEII G'GTnAC_C Cuts at: 1184 2885 1184 Size: 1701 4465 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2671 2122 Size: 549 5617 BstYI r'GATC_y Cuts at: 1972 2145 2781 3496 3863 4248 4265 5717 5803 Size: 173 636 715 367 385 17 1452 86 Cuts at: 5803 5815 6161 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 715 636 385 367 346 173 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3462 3462 Size: 6166 BtgI C'CryG_G Cuts at: 513 1065 2004 2547 513 Size: 552 939 543 4132 Fragments arranged by size: 4132 939 552 543 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5427 Fragments arranged by size: 5427 683 56 BtsI GCAGTG_nn' Cuts at: 3682 4476 4496 3682 Size: 794 20 5352 Fragments arranged by size: 5352 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6166 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6131 DraI TTT'AAA Cuts at: 3470 3470 Size: 6166 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5173 809 Size: 507 3857 1802 Fragments arranged by size: 3857 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2466 2550 2568 Size: 54 1425 50 275 18 636 84 18 Cuts at: 2568 2766 3613 4502 6131 8 Size: 198 847 889 1629 43 Fragments arranged by size: 1629 1425 889 847 636 275 198 84 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3613 1537 Size: 275 1801 4090 Fragments arranged by size: 4090 1801 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2342 4096 1353 Size: 989 1754 3423 Fragments arranged by size: 3423 1754 989 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4753 5127 5273 387 Size: 1064 138 3164 374 146 1280 Fragments arranged by size: 3164 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3039 3407 4229 4826 5613 5881 1239 Size: 393 1407 368 822 597 787 268 1524 Fragments arranged by size: 1524 1407 822 787 597 393 368 268 EcoNI CCTnn'n_nnAGG Cuts at: 2306 2306 Size: 6166 EcoO109I rG'GnC_Cy Cuts at: 2192 2193 2224 2961 2192 Size: 1 31 737 5397 Fragments arranged by size: 5397 737 31 1 EcoRI G'AATT_C Cuts at: 3475 3853 3475 Size: 378 5788 FspI TGC'GCA Cuts at: 4672 4672 Size: 6166 HaeII r_GCGC'y Cuts at: 1678 2646 3049 5313 1678 Size: 968 403 2264 2531 Fragments arranged by size: 2531 2264 968 403 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2094 3104 3136 Size: 32 135 32 458 32 32 1010 32 Cuts at: 3136 4807 4839 4881 4913 1373 Size: 1671 32 42 32 2626 Fragments arranged by size: 2626 1671 1010 458 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3510 3756 669 Size: 988 281 1572 246 3079 Fragments arranged by size: 3079 1572 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6166 HpaI GTT'AAC Cuts at: 3756 3756 Size: 6166 KasI G'GCGC_C Cuts at: 2642 3045 2642 Size: 403 5763 KpnI G_GTAC'C Cuts at: 3491 3491 Size: 6166 MfeI C'AATT_G Cuts at: 3765 3765 Size: 6166 MluI A'CGCG_T Cuts at: 6042 6042 Size: 6166 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5280 5464 1305 Size: 3975 184 2007 Fragments arranged by size: 3975 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2570 2768 10 Size: 54 1425 343 738 198 3408 Fragments arranged by size: 3408 1425 738 343 198 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2810 2816 2834 2990 3188 3266 Size: 648 954 690 6 18 156 198 78 Cuts at: 3266 4124 4483 4642 518 Size: 858 359 159 2042 Fragments arranged by size: 2042 954 858 690 648 359 198 159 156 78 18 6 MspA1I CmG'CkG Cuts at: 1606 2549 2586 2650 2975 3582 4259 5407 5652 Size: 943 37 64 325 607 677 1148 245 Cuts at: 5652 1606 Size: 2120 Fragments arranged by size: 2120 1148 943 677 607 325 245 64 37 NarI GG'CG_CC Cuts at: 2643 3046 2643 Size: 403 5763 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4675 Fragments arranged by size: 4675 939 552 NdeI CA'TA_TG Cuts at: 387 2207 387 Size: 1820 4346 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6166 NotI GC'GGCC_GC Cuts at: 3613 3613 Size: 6166 NruI TCG'CGA Cuts at: 2231 2231 Size: 6166 NspI r_CATG'y Cuts at: 1132 1229 3016 3524 5069 1132 Size: 97 1787 508 1545 2229 Fragments arranged by size: 2229 1787 1545 508 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6166 PciI A'CATG_T Cuts at: 5065 5065 Size: 6166 PflMI CCAn_nnn'nTGG Cuts at: 1108 2869 1108 Size: 1761 4405 PmeI GTTT'AAAC Cuts at: 3470 3470 Size: 6166 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4235 4267 5748 5780 4235 Size: 32 1481 32 4621 Fragments arranged by size: 4621 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2224 2961 2224 Size: 737 5429 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6166 PsiI TTA'TAA Cuts at: 3736 3736 Size: 6166 PspOMI G'GGCC_C Cuts at: 2192 2318 2192 Size: 126 6040 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2807 2839 2807 Size: 32 6134 PstI C_TGCA'G Cuts at: 830 2859 3518 830 Size: 2029 659 3478 Fragments arranged by size: 3478 2029 659 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6166 PvuII CAG'CTG Cuts at: 2650 2650 Size: 6166 SacII CC_GC'GG Cuts at: 2550 2550 Size: 6166 SalI G'TCGA_C Cuts at: 1936 3508 1936 Size: 1572 4594 SbfI CC_TGCA'GG Cuts at: 3518 3518 Size: 6166 ScaI AGT'ACT Cuts at: 4414 6126 4414 Size: 1712 4454 SfcI C'TryA_G Cuts at: 826 1045 2855 3514 4649 5330 5521 826 Size: 219 1810 659 1135 681 191 1471 Fragments arranged by size: 1810 1471 1135 681 659 219 191 SfoI GGC'GCC Cuts at: 2644 3047 2644 Size: 403 5763 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6166 SmaI CCC'GGG Cuts at: 1872 3493 1872 Size: 1621 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4270 5171 5433 820 Size: 197 926 2327 901 262 1553 Fragments arranged by size: 2327 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3957 493 Size: 3464 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6166 SphI G_CATG'C Cuts at: 1132 3524 1132 Size: 2392 3774 SspI AAT'ATT Cuts at: 5 52 4090 5 Size: 47 4038 2081 Fragments arranged by size: 4038 2081 47 StuI AGG'CCT Cuts at: 1517 2312 3220 1517 Size: 795 908 4463 Fragments arranged by size: 4463 908 795 StyI C'CwwG_G Cuts at: 513 1065 2004 2605 3605 513 Size: 552 939 601 1000 3074 Fragments arranged by size: 3074 1000 939 601 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2228 4193 4210 4363 4548 4993 325 Size: 1255 648 1965 17 153 185 445 1498 Fragments arranged by size: 1965 1498 1255 648 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4412 6124 96 Size: 274 80 33 51 1443 2435 1712 138 Fragments arranged by size: 2435 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3171 3203 6126 6158 3171 Size: 32 2923 32 3179 Fragments arranged by size: 3179 2923 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6166 XbaI T'CTAG_A Cuts at: 3502 3502 Size: 6166 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3377 3377 Size: 6166 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6166 XmaI C'CCGG_G Cuts at: 1870 3491 1870 Size: 1621 4545 XmnI GAAnn'nnTTC Cuts at: 2540 4295 2540 Size: 1755 4411 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3974 276 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PspXI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI