(Circular) (Six-base) MAPSORT of: pFN21AE1331.seq Check: 3515 from: 1 to: 5713 pFN21AE1331 5713 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3523 278 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3056 1937 Size: 1119 4594 Acc65I G'GTAC_C Cuts at: 3034 3034 Size: 5713 AclI AA'CG_TT Cuts at: 1563 3840 4213 1563 Size: 2277 373 3063 Fragments arranged by size: 3063 2277 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2314 2633 2717 2991 3776 5160 5428 1632 Size: 682 319 84 274 785 1384 268 1917 Fragments arranged by size: 1917 1384 785 682 319 274 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5516 AflIII A'CryG_T Cuts at: 1203 2828 4612 5589 1203 Size: 1625 1784 977 1327 Fragments arranged by size: 1784 1625 1327 977 AgeI A'CCGG_T Cuts at: 4544 4544 Size: 5713 AhdI GACnn_n'nnGTC Cuts at: 4442 4442 Size: 5713 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5713 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2357 2966 1184 Size: 1173 609 3931 Fragments arranged by size: 3931 1173 609 AlwNI CAG_nnn'CTG Cuts at: 2848 3456 5028 2848 Size: 608 1572 3533 Fragments arranged by size: 3533 1572 608 ApaLI G'TGCA_C Cuts at: 3770 4926 3770 Size: 1156 4557 ApoI r'AATT_y Cuts at: 1493 2083 2520 3022 3248 3400 5509 1493 Size: 590 437 502 226 152 2109 1697 Fragments arranged by size: 2109 1697 590 502 437 226 152 AseI AT'TA_AT Cuts at: 160 1032 4267 160 Size: 872 3235 1606 Fragments arranged by size: 3235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 AvaI C'yCGr_G Cuts at: 1870 1943 3038 1870 Size: 73 1095 4545 Fragments arranged by size: 4545 1095 73 BamHI G'GATC_C Cuts at: 3043 3410 3043 Size: 367 5346 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2129 2341 2448 3034 4489 Size: 325 217 627 342 212 107 586 1455 Cuts at: 4489 618 Size: 1842 Fragments arranged by size: 1842 1455 627 586 342 325 217 212 107 BanII G_rGCy'C Cuts at: 1391 2034 2511 3032 1391 Size: 643 477 521 4072 Fragments arranged by size: 4072 643 521 477 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4742 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3904 3938 1903 Size: 34 1967 34 3678 Fragments arranged by size: 3678 1967 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2231 3605 4815 2231 Size: 1374 1210 3129 Fragments arranged by size: 3129 1374 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5713 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2843 4324 136 Size: 107 122 71 631 605 1171 1481 1525 Fragments arranged by size: 1525 1481 1171 631 605 122 107 71 BglII A'GATC_T Cuts at: 5708 5708 Size: 5713 BlpI GC'TnA_GC Cuts at: 2419 3130 2419 Size: 711 5002 Bme1580I G_kGCm'C Cuts at: 1790 2326 2346 3774 4930 1790 Size: 536 20 1428 1156 2573 Fragments arranged by size: 2573 1428 1156 536 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5713 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2483 3444 4402 Size: 416 53 83 399 24 1066 961 958 Cuts at: 4402 442 Size: 1753 Fragments arranged by size: 1753 1066 961 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2232 1056 Size: 1176 4537 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4373 1239 Size: 3134 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2038 3838 4703 5001 2038 Size: 1800 865 298 2750 Fragments arranged by size: 2750 1800 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4376 882 Size: 617 2877 2219 Fragments arranged by size: 2877 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3504 493 Size: 711 2300 2702 Fragments arranged by size: 2702 2300 711 BsaBI GATnn'nnATC Cuts at: 2735 5707 2735 Size: 2972 2741 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3520 3902 4537 275 Size: 53 83 186 936 1987 382 635 1451 Fragments arranged by size: 1987 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4146 4544 4818 4965 5376 4146 Size: 398 274 147 411 4483 Fragments arranged by size: 4483 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5488 Fragments arranged by size: 5488 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2903 1186 Size: 1717 3996 BseYI C'CCAG_C Cuts at: 2838 4916 5412 2838 Size: 2078 496 3139 Fragments arranged by size: 3139 2078 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2003 2169 2499 2572 1163 Size: 573 267 166 330 73 4304 Fragments arranged by size: 4304 573 330 267 166 73 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3163 3924 4952 1540 Size: 275 186 1162 761 1028 2301 Fragments arranged by size: 2301 1162 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2034 3774 3859 4930 2034 Size: 1740 85 1071 2817 Fragments arranged by size: 2817 1740 1071 85 BsmI GAATG_Cn' Cuts at: 3224 3317 3224 Size: 93 5620 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2326 2346 2511 3032 3774 3859 Size: 399 244 292 20 165 521 742 85 Cuts at: 3859 4930 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 521 399 292 244 165 85 20 BspEI T'CCGG_A Cuts at: 5376 5376 Size: 5713 BspHI T'CATG_A Cuts at: 3602 5332 3602 Size: 1730 3983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 2862 3068 844 Size: 1334 684 206 3489 Fragments arranged by size: 3489 1334 684 206 BsrBI CCG'CTC Cuts at: 1394 1823 3600 1394 Size: 429 1777 3507 Fragments arranged by size: 3507 1777 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4208 4382 65 Size: 1183 2960 174 1396 Fragments arranged by size: 2960 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4357 4544 1700 Size: 110 2547 187 2869 Fragments arranged by size: 2869 2547 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5713 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5713 BssSI C'ACGA_G Cuts at: 3773 4785 3773 Size: 1012 4701 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5713 BstBI TT'CG_AA Cuts at: 2070 4517 2070 Size: 2447 3266 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5713 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2727 2215 Size: 512 5201 BstYI r'GATC_y Cuts at: 1972 3043 3410 3795 3812 5264 5350 5362 5708 Size: 1071 367 385 17 1452 86 12 346 Cuts at: 5708 1972 Size: 1977 Fragments arranged by size: 1977 1452 1071 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4222 Fragments arranged by size: 4222 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4974 Fragments arranged by size: 4974 683 56 BtsI GCAGTG_nn' Cuts at: 2186 2326 2546 3229 4023 4043 2186 Size: 140 220 683 794 20 3856 Fragments arranged by size: 3856 794 683 220 140 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5678 DraI TTT'AAA Cuts at: 3017 3017 Size: 5713 DraIII CAC_nnn'GTG Cuts at: 2896 2896 Size: 5713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4720 809 Size: 507 3404 1802 Fragments arranged by size: 3404 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2562 3160 4049 Size: 54 1425 50 275 18 732 598 889 Cuts at: 4049 5678 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 732 598 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3160 1537 Size: 275 1348 4090 Fragments arranged by size: 4090 1348 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2249 2270 2333 3643 1353 Size: 896 21 63 1310 3423 Fragments arranged by size: 3423 1310 896 63 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 4300 4674 4820 387 Size: 1064 138 453 2258 374 146 1280 Fragments arranged by size: 2258 1280 1064 453 374 146 138 EcoICRI GAG'CTC Cuts at: 2032 2032 Size: 5713 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2314 2633 2717 2991 3776 4373 5160 Size: 393 682 319 84 274 785 597 787 Cuts at: 5160 5428 1239 Size: 268 1524 Fragments arranged by size: 1524 787 785 682 597 393 319 274 268 84 EcoO109I rG'GnC_Cy Cuts at: 2488 2723 2488 Size: 235 5478 EcoRI G'AATT_C Cuts at: 2083 3022 3400 2083 Size: 939 378 4396 Fragments arranged by size: 4396 939 378 FspI TGC'GCA Cuts at: 4219 4219 Size: 5713 HaeII r_GCGC'y Cuts at: 1678 4860 1678 Size: 3182 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4354 4386 4428 4460 1373 Size: 32 135 32 2782 32 42 32 2626 Fragments arranged by size: 2782 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2826 3057 3303 669 Size: 988 281 888 231 246 3079 Fragments arranged by size: 3079 988 888 281 246 231 HindIII A'AGCT_T Cuts at: 748 748 Size: 5713 HpaI GTT'AAC Cuts at: 3303 3303 Size: 5713 KpnI G_GTAC'C Cuts at: 3038 3038 Size: 5713 MfeI C'AATT_G Cuts at: 3312 3312 Size: 5713 MluI A'CGCG_T Cuts at: 5589 5589 Size: 5713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4827 5011 1305 Size: 3522 184 2007 Fragments arranged by size: 3522 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2564 10 Size: 54 1425 343 732 3159 Fragments arranged by size: 3159 1425 732 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2339 3671 4030 4189 518 Size: 648 1173 1332 359 159 2042 Fragments arranged by size: 2042 1332 1173 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 2147 2271 2308 3129 3806 4954 5199 Size: 456 85 124 37 821 677 1148 245 Cuts at: 5199 1606 Size: 2120 Fragments arranged by size: 2120 1148 821 677 456 245 124 85 37 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4222 Fragments arranged by size: 4222 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5713 NheI G'CTAG_C Cuts at: 1052 2228 1052 Size: 1176 4537 NotI GC'GGCC_GC Cuts at: 3160 3160 Size: 5713 NspI r_CATG'y Cuts at: 1132 1229 2832 3071 4616 1132 Size: 97 1603 239 1545 2229 Fragments arranged by size: 2229 1603 1545 239 97 PasI CC'CwG_GG Cuts at: 1348 2357 2461 2503 1348 Size: 1009 104 42 4558 Fragments arranged by size: 4558 1009 104 42 PciI A'CATG_T Cuts at: 2828 4612 2828 Size: 1784 3929 PflMI CCAn_nnn'nTGG Cuts at: 1108 2473 1108 Size: 1365 4348 PmeI GTTT'AAAC Cuts at: 3017 3017 Size: 5713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3782 3814 5295 5327 3782 Size: 32 1481 32 4168 Fragments arranged by size: 4168 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2488 2488 Size: 5713 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5713 PsiI TTA'TAA Cuts at: 3283 3283 Size: 5713 PstI C_TGCA'G Cuts at: 830 3065 830 Size: 2235 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5713 PvuII CAG'CTG Cuts at: 2062 2147 2308 2062 Size: 85 161 5467 Fragments arranged by size: 5467 161 85 SacI G_AGCT'C Cuts at: 2034 2034 Size: 5713 SalI G'TCGA_C Cuts at: 1936 3055 1936 Size: 1119 4594 SbfI CC_TGCA'GG Cuts at: 3065 3065 Size: 5713 ScaI AGT'ACT Cuts at: 3961 5673 3961 Size: 1712 4001 SfcI C'TryA_G Cuts at: 826 1045 2637 3061 4196 4877 5068 826 Size: 219 1592 424 1135 681 191 1471 Fragments arranged by size: 1592 1471 1135 681 424 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5713 SmaI CCC'GGG Cuts at: 1872 3040 1872 Size: 1168 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2053 3817 4718 4980 820 Size: 197 926 110 1764 901 262 1553 Fragments arranged by size: 1764 1553 926 901 262 197 110 SnaBI TAC'GTA Cuts at: 493 3504 493 Size: 3011 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5713 SphI G_CATG'C Cuts at: 1132 3071 1132 Size: 1939 3774 SspI AAT'ATT Cuts at: 5 52 3637 5 Size: 47 3585 2081 Fragments arranged by size: 3585 2081 47 StuI AGG'CCT Cuts at: 1517 2496 1517 Size: 979 4734 StyI C'CwwG_G Cuts at: 513 1065 2004 2688 2998 3152 513 Size: 552 939 684 310 154 3074 Fragments arranged by size: 3074 939 684 552 310 154 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2465 3740 3757 3910 4095 4540 325 Size: 1255 885 1275 17 153 185 445 1498 Fragments arranged by size: 1498 1275 1255 885 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3959 5671 96 Size: 274 80 33 51 1443 1982 1712 138 Fragments arranged by size: 1982 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5673 5705 5673 Size: 32 5681 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5713 XbaI T'CTAG_A Cuts at: 3049 3049 Size: 5713 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5713 XmaI C'CCGG_G Cuts at: 1870 3038 1870 Size: 1168 4545 XmnI GAAnn'nnTTC Cuts at: 3842 3842 Size: 5713 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3521 276 Size: 53 83 186 936 1987 2468 Fragments arranged by size: 2468 1987 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI