################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BbrPI BcgI BciVI BfaI BfrI BlnI BsaAI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I ClaI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KpnI KspI MaeI MaeII MfeI MluI MluNI MroI MscI MseI MunI MvnI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp45I Tsp509I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AviII 710 TGCGCA TGC^GCA BbeI 259 GGCGCC GGCGC^C BbsI 204 GAAGAC GAAGAC(2/6) BbvCI 62 CCTCAGC CCTCAGC(-5/-2) BclI 401 TGATCA T^GATCA BglII 683 AGATCT A^GATCT BlpI 582 GCTGAGC GC^T[ACGT]AGC Bpu1102I 582 GCTGAGC GC^T[ACGT]AGC BpuAI 204 GAAGAC GAAGAC(2/6) BstAPI 702 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1210 CCACCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 582 GCTGAGC GC^T[ACGT]AGC CpoI 98 CGGACCG CG^G[AT]CCG DraIII 211 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 37 GACTGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 401 TGATCA T^GATCA FspI 710 TGCGCA TGC^GCA HaeII 259 GGCGCC [GA]GCGC^[CT] HphI 789 GGTGA GGTGA(8/7) KasI 259 GGCGCC G^GCGCC MaeIII 411 GTTAC ^GT[ACGT]AC MslI 183 CATTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 259 GGCGCC GG^CGCC NspI 396 GCATGT [GA]CATG^[CT] PaeR7I 1171 CTCGAG C^TCGAG PflMI 641 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsrII 98 CGGACCG CG^G[AT]CCG SfoI 259 GGCGCC GGC^GCC SmaI 107 CCCGGG CCC^GGG SmlI 1171 CTCGAG C^T[CT][GA]AG Van91I 641 CCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1171 CTCGAG C^TCGAG XmaCI 107 CCCGGG C^CCGGG XmaI 107 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 3 GGCGTC G[GA]^CG[CT]C 259 GGCGCC Alw44I 174 GTGCAC G^TGCAC 904 GTGCAC ApaI 560 GGGCCC GGGCC^C 1205 GGGCCC ApaLI 174 GTGCAC G^TGCAC 904 GTGCAC BamHI 548 GGATCC G^GATCC 885 GGATCC BanI 259 GGCGCC G^G[CT][GA]CC 664 GGTGCC BglI 67 GCCATCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 382 GCCCAGGAGGC BsaBI 795 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1114 GATCAGGATC BsaHI 3 GGCGTC G[GA]^CG[CT]C 259 GGCGCC BsgI 539 CTGCAC GTGCAG(16/14) 118 GTGCAG BsrFI 164 GCCGGC [GA]^CCGG[CT] 1195 GCCGGC Cfr10I 164 GCCGGC [GA]^CCGG[CT] 1195 GCCGGC DsaI 854 CCACGG C^C[GA][CT]GG 1077 CCGTGG EaeI 857 CGGCCA [CT]^GGCC[GA] 1193 TGGCCG EarI 1234 CTCTTC CTCTTC(1/4) 1251 GAAGAG EcoNI 876 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1233 CCTCTTCCAGG EcoT14I 641 CCAAGG C^C[AT][AT]GG 725 CCAAGG HgaI 207 GACGC GACGC(5/10) 4 GCGTC Hin1I 3 GGCGTC G[GA]^CG[CT]C 259 GGCGCC Ksp632I 1234 CTCTTC CTCTTC(1/4) 1251 GAAGAG MamI 795 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1114 GATCAGGATC MspA1I 521 CAGCTG C[AC]G^C[GT]G 580 CAGCTG NaeI 164 GCCGGC GCC^GGC 1195 GCCGGC NgoMIV 164 GCCGGC G^CCGGC 1195 GCCGGC PvuII 521 CAGCTG CAG^CTG 580 CAGCTG StyI 641 CCAAGG C^C[AT][AT]GG 725 CCAAGG TfiI 219 GATTC G^A[AT]TC 1040 GATTC TspRI 172 CAGTG CA[GC]TG[ACGT][ACGT]^ 498 CACTG XcmI 579 CCAGCTGAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 854 CCACGGCCAGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StuI TaqI TseI TthHB8I XhoII