(Circular) (Six-base) MAPSORT of: pFN21AE6311.seq Check: 9940 from: 1 to: 5980 pFN21AE6311 5980 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3790 278 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3323 1937 Size: 1386 4594 Acc65I G'GTAC_C Cuts at: 3301 3301 Size: 5980 AclI AA'CG_TT Cuts at: 1563 4107 4480 1563 Size: 2544 373 3063 Fragments arranged by size: 3063 2544 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4043 5427 5695 1632 Size: 2411 1384 268 1917 Fragments arranged by size: 2411 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5783 AflIII A'CryG_T Cuts at: 1203 4879 5856 1203 Size: 3676 977 1327 Fragments arranged by size: 3676 1327 977 AgeI A'CCGG_T Cuts at: 4811 4811 Size: 5980 AhdI GACnn_n'nnGTC Cuts at: 4709 4709 Size: 5980 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5980 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2504 1184 Size: 1320 4660 AlwNI CAG_nnn'CTG Cuts at: 2998 3160 3193 3723 5295 2998 Size: 162 33 530 1572 3683 Fragments arranged by size: 3683 1572 530 162 33 ApaI G_GGCC'C Cuts at: 2569 3214 2569 Size: 645 5335 ApaLI G'TGCA_C Cuts at: 2179 2909 4037 5193 2179 Size: 730 1128 1156 2966 Fragments arranged by size: 2966 1156 1128 730 ApoI r'AATT_y Cuts at: 1493 3289 3515 3667 5776 1493 Size: 1796 226 152 2109 1697 Fragments arranged by size: 2109 1796 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4534 160 Size: 872 3502 1606 Fragments arranged by size: 3502 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 AvaI C'yCGr_G Cuts at: 1870 1943 2112 2842 3176 3305 1870 Size: 73 169 730 334 129 4545 Fragments arranged by size: 4545 730 334 169 129 73 BamHI G'GATC_C Cuts at: 2553 2890 3310 3677 2553 Size: 337 420 367 4856 Fragments arranged by size: 4856 420 367 337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2264 2669 3301 4756 618 Size: 325 217 627 477 405 632 1455 1842 Fragments arranged by size: 1842 1455 632 627 477 405 325 217 BanII G_rGCy'C Cuts at: 1391 2021 2569 3214 3299 1391 Size: 630 548 645 85 4072 Fragments arranged by size: 4072 645 630 548 85 BbeI G_GCGC'C Cuts at: 2268 2268 Size: 5980 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 928 Size: 971 317 4692 Fragments arranged by size: 4692 971 317 BbvCI CC'TCA_GC Cuts at: 2068 2068 Size: 5980 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4171 4205 1903 Size: 34 2234 34 3678 Fragments arranged by size: 3678 2234 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3872 5082 3872 Size: 1210 4770 BclI T'GATC_A Cuts at: 1548 2406 1548 Size: 858 5122 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2078 2393 4591 Size: 107 122 71 631 605 406 315 2198 Cuts at: 4591 136 Size: 1525 Fragments arranged by size: 2198 1525 631 605 406 315 122 107 71 BglII A'GATC_T Cuts at: 2688 5975 2688 Size: 3287 2693 BlpI GC'TnA_GC Cuts at: 2588 3397 2588 Size: 809 5171 Bme1580I G_kGCm'C Cuts at: 1790 2061 2183 2569 2913 3214 4041 5197 1790 Size: 271 122 386 344 301 827 1156 2573 Fragments arranged by size: 2573 1156 827 386 344 301 271 122 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5980 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3711 4669 442 Size: 416 53 83 399 24 2294 958 1753 Fragments arranged by size: 2294 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5980 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 2441 2471 2540 3125 3182 4640 1239 Size: 920 282 30 69 585 57 1458 2579 Fragments arranged by size: 2579 1458 920 585 282 69 57 30 Bpu10I CC'TnA_GC Cuts at: 2068 2068 Size: 5980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4105 4970 5268 4105 Size: 865 298 4817 Fragments arranged by size: 4817 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4643 882 Size: 617 3144 2219 Fragments arranged by size: 3144 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3771 493 Size: 711 2567 2702 Fragments arranged by size: 2702 2567 711 BsaBI GATnn'nnATC Cuts at: 2804 3123 5974 2804 Size: 319 2851 2810 Fragments arranged by size: 2851 2810 319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2265 3787 4169 Size: 53 83 186 936 476 256 1522 382 Cuts at: 4169 4804 275 Size: 635 1451 Fragments arranged by size: 1522 1451 936 635 476 382 256 186 83 53 BsaWI w'CCGG_w Cuts at: 4413 4811 5085 5232 5643 4413 Size: 398 274 147 411 4750 Fragments arranged by size: 4750 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2143 2150 2173 1178 Size: 30 165 30 717 23 7 23 4985 Fragments arranged by size: 4985 717 165 30 30 23 23 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5980 BseYI C'CCAG_C Cuts at: 2341 2528 2703 3229 5183 5679 2341 Size: 187 175 526 1954 496 2642 Fragments arranged by size: 2642 1954 526 496 187 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 2529 1163 Size: 573 408 385 4614 Fragments arranged by size: 4614 573 408 385 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3430 4191 5219 1540 Size: 275 186 1429 761 1028 2301 Fragments arranged by size: 2301 1429 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2183 2427 2913 3019 4041 4126 5197 2183 Size: 244 486 106 1022 85 1071 2966 Fragments arranged by size: 2966 1071 1022 486 244 106 85 BsmI GAATG_Cn' Cuts at: 3491 3584 3491 Size: 93 5887 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2061 2183 2427 2569 2913 3019 Size: 399 231 40 122 244 142 344 106 Cuts at: 3019 3214 3299 4041 4126 5197 1391 Size: 195 85 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 344 244 231 195 142 122 106 85 85 40 BspEI T'CCGG_A Cuts at: 5643 5643 Size: 5980 BspHI T'CATG_A Cuts at: 3869 5599 3869 Size: 1730 4250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3335 844 Size: 2491 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3867 1394 Size: 429 2044 3507 Fragments arranged by size: 3507 2044 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4475 4649 65 Size: 1183 3227 174 1396 Fragments arranged by size: 3227 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2169 3200 4624 4811 1700 Size: 110 359 1031 1424 187 2869 Fragments arranged by size: 2869 1424 1031 359 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5980 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5980 BssSI C'ACGA_G Cuts at: 4040 5052 4040 Size: 1012 4968 BstAPI GCAn_nnn'nTGC Cuts at: 794 2713 794 Size: 1919 4061 BstBI TT'CG_AA Cuts at: 4784 4784 Size: 5980 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5980 BstXI CCAn_nnnn'nTGG Cuts at: 3222 3222 Size: 5980 BstYI r'GATC_y Cuts at: 1972 2553 2688 2890 3157 3310 3677 4062 4079 Size: 581 135 202 267 153 367 385 17 Cuts at: 4079 5531 5617 5629 5975 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 581 385 367 346 267 202 153 135 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2859 3082 513 Size: 552 939 855 223 3411 Fragments arranged by size: 3411 939 855 552 223 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5241 Fragments arranged by size: 5241 683 56 BtsI GCAGTG_nn' Cuts at: 3496 4290 4310 3496 Size: 794 20 5166 Fragments arranged by size: 5166 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5980 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2806 2841 529 Size: 35 2242 35 3668 Fragments arranged by size: 3668 2242 35 35 DraI TTT'AAA Cuts at: 3284 3284 Size: 5980 DraIII CAC_nnn'GTG Cuts at: 2221 2221 Size: 5980 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2048 4987 809 Size: 507 732 2939 1802 Fragments arranged by size: 2939 1802 732 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2862 3198 3427 Size: 54 1425 50 275 18 1032 336 229 Cuts at: 3427 4316 5945 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1032 889 336 275 229 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3427 1537 Size: 275 1615 4090 Fragments arranged by size: 4090 1615 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3245 3251 3910 1353 Size: 1892 6 659 3423 Fragments arranged by size: 3423 1892 659 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4567 4941 5087 387 Size: 1064 138 2978 374 146 1280 Fragments arranged by size: 2978 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2441 2471 2540 3125 3182 4043 Size: 393 527 282 30 69 585 57 861 Cuts at: 4043 4640 5427 5695 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 861 787 597 585 527 393 282 268 69 57 30 EcoNI CCTnn'n_nnAGG Cuts at: 2885 3242 2885 Size: 357 5623 EcoO109I rG'GnC_Cy Cuts at: 2565 2986 3211 2565 Size: 421 225 5334 Fragments arranged by size: 5334 421 225 EcoRI G'AATT_C Cuts at: 3289 3667 3289 Size: 378 5602 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2755 2755 Size: 5980 FspI TGC'GCA Cuts at: 2717 4486 2717 Size: 1769 4211 FspAI rTGC'GCAy Cuts at: 2717 2717 Size: 5980 HaeII r_GCGC'y Cuts at: 1678 2268 5127 1678 Size: 590 2859 2531 Fragments arranged by size: 2859 2531 590 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4621 4653 4695 4727 1373 Size: 32 135 32 3049 32 42 32 2626 Fragments arranged by size: 3049 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3324 3570 669 Size: 988 281 1386 246 3079 Fragments arranged by size: 3079 1386 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5980 HpaI GTT'AAC Cuts at: 3570 3570 Size: 5980 KasI G'GCGC_C Cuts at: 2264 2264 Size: 5980 KpnI G_GTAC'C Cuts at: 3305 3305 Size: 5980 MfeI C'AATT_G Cuts at: 3579 3579 Size: 5980 MluI A'CGCG_T Cuts at: 5856 5856 Size: 5980 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2604 5094 5278 1305 Size: 1299 2490 184 2007 Fragments arranged by size: 2490 2007 1299 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4158 Fragments arranged by size: 4158 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 3938 4297 4456 518 Size: 648 1026 1746 359 159 2042 Fragments arranged by size: 2042 1746 1026 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2528 2587 3396 4073 5221 5466 1606 Size: 922 59 809 677 1148 245 2120 Fragments arranged by size: 2120 1148 922 809 677 245 59 NaeI GCC'GGC Cuts at: 2171 3202 2171 Size: 1031 4949 NarI GG'CG_CC Cuts at: 2265 2265 Size: 5980 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4489 Fragments arranged by size: 4489 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5980 NgoMIV G'CCGG_C Cuts at: 2169 3200 2169 Size: 1031 4949 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5980 NotI GC'GGCC_GC Cuts at: 3427 3427 Size: 5980 NspI r_CATG'y Cuts at: 1132 1229 2405 3338 4883 1132 Size: 97 1176 933 1545 2229 Fragments arranged by size: 2229 1545 1176 933 97 PasI CC'CwG_GG Cuts at: 1348 3100 1348 Size: 1752 4228 PciI A'CATG_T Cuts at: 4879 4879 Size: 5980 PflMI CCAn_nnn'nTGG Cuts at: 1108 2652 1108 Size: 1544 4436 PfoI T'CCnGG_A Cuts at: 2556 2556 Size: 5980 PmeI GTTT'AAAC Cuts at: 3284 3284 Size: 5980 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4049 4081 5562 5594 4049 Size: 32 1481 32 4435 Fragments arranged by size: 4435 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5980 PsiI TTA'TAA Cuts at: 3550 3550 Size: 5980 PspOMI G'GGCC_C Cuts at: 2565 3210 2565 Size: 645 5335 PspXI vC'TCGA_Gb Cuts at: 3176 3176 Size: 5980 PstI C_TGCA'G Cuts at: 830 3332 830 Size: 2502 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5980 PvuII CAG'CTG Cuts at: 2528 2587 2528 Size: 59 5921 RsrII CG'GwC_CG Cuts at: 2104 2104 Size: 5980 SalI G'TCGA_C Cuts at: 1936 3322 1936 Size: 1386 4594 SbfI CC_TGCA'GG Cuts at: 3332 3332 Size: 5980 ScaI AGT'ACT Cuts at: 4228 5940 4228 Size: 1712 4268 SfcI C'TryA_G Cuts at: 826 1045 3328 4463 5144 5335 826 Size: 219 2283 1135 681 191 1471 Fragments arranged by size: 2283 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2266 2266 Size: 5980 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5980 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5980 SmaI CCC'GGG Cuts at: 1872 2114 3307 1872 Size: 242 1193 4545 Fragments arranged by size: 4545 1193 242 SmlI C'TyrA_G Cuts at: 820 1017 1943 3176 4084 4985 5247 820 Size: 197 926 1233 908 901 262 1553 Fragments arranged by size: 1553 1233 926 908 901 262 197 SnaBI TAC'GTA Cuts at: 493 3771 493 Size: 3278 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5980 SphI G_CATG'C Cuts at: 1132 3338 1132 Size: 2206 3774 SspI AAT'ATT Cuts at: 5 52 3904 5 Size: 47 3852 2081 Fragments arranged by size: 3852 2081 47 StuI AGG'CCT Cuts at: 1517 2396 3174 3248 1517 Size: 879 778 74 4249 Fragments arranged by size: 4249 879 778 74 StyI C'CwwG_G Cuts at: 513 1065 2004 2646 2730 3419 513 Size: 552 939 642 84 689 3074 Fragments arranged by size: 3074 939 689 642 552 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2121 2503 4007 4024 4177 4362 4807 Size: 1255 541 382 1504 17 153 185 445 Cuts at: 4807 325 Size: 1498 Fragments arranged by size: 1504 1498 1255 541 445 382 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4226 5938 96 Size: 274 80 33 51 1443 2249 1712 138 Fragments arranged by size: 2249 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2143 2175 2538 2570 5940 5972 2143 Size: 32 363 32 3370 32 2151 Fragments arranged by size: 3370 2151 363 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5980 XbaI T'CTAG_A Cuts at: 3316 3316 Size: 5980 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2591 2866 2591 Size: 275 5705 XhoI C'TCGA_G Cuts at: 1943 3176 1943 Size: 1233 4747 XmaI C'CCGG_G Cuts at: 1870 2112 3305 1870 Size: 242 1193 4545 Fragments arranged by size: 4545 1193 242 XmnI GAAnn'nnTTC Cuts at: 4109 4109 Size: 5980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3788 276 Size: 53 83 186 936 2254 2468 Fragments arranged by size: 2468 2254 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI NruI NsiI PacI PmlI PpuMI PsrI SacI SacII SanDI SapI SexAI SfiI SrfI SwaI