(Linear) (Six-base) MAPSORT of: KIEE6311.seq Check: 8998 from: 1 to: 1275 KIEE6311.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 499 1275 Size: 499 776 AlwNI CAG_nnn'CTG Cuts at: 0 993 1155 1188 1275 Size: 993 162 33 87 Fragments arranged by size: 993 162 87 33 ApaI G_GGCC'C Cuts at: 0 564 1209 1275 Size: 564 645 66 Fragments arranged by size: 645 564 66 ApaLI G'TGCA_C Cuts at: 0 174 904 1275 Size: 174 730 371 Fragments arranged by size: 730 371 174 AvaI C'yCGr_G Cuts at: 0 107 837 1171 1275 Size: 107 730 334 104 Fragments arranged by size: 730 334 107 104 BamHI G'GATC_C Cuts at: 0 548 885 1275 Size: 548 337 390 Fragments arranged by size: 548 390 337 BanI G'GyrC_C Cuts at: 0 259 664 1275 Size: 259 405 611 Fragments arranged by size: 611 405 259 BanII G_rGCy'C Cuts at: 0 16 564 1209 1275 Size: 16 548 645 66 Fragments arranged by size: 645 548 66 16 BbeI G_GCGC'C Cuts at: 0 263 1275 Size: 263 1012 BbsI GAAGACnn'nnnn_ Cuts at: 0 211 1275 Size: 211 1064 BbvCI CC'TCA_GC Cuts at: 0 63 1275 Size: 63 1212 BclI T'GATC_A Cuts at: 0 401 1275 Size: 401 874 BglI GCCn_nnn'nGGC Cuts at: 0 73 388 1275 Size: 73 315 887 Fragments arranged by size: 887 315 73 BglII A'GATC_T Cuts at: 0 683 1275 Size: 683 592 BlpI GC'TnA_GC Cuts at: 0 583 1275 Size: 583 692 Bme1580I G_kGCm'C Cuts at: 0 56 178 564 908 1209 1275 Size: 56 122 386 344 301 66 Fragments arranged by size: 386 344 301 122 66 56 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 436 466 535 1120 1177 1275 Size: 154 282 30 69 585 57 98 Fragments arranged by size: 585 282 154 98 69 57 30 Bpu10I CC'TnA_GC Cuts at: 0 63 1275 Size: 63 1212 BsaBI GATnn'nnATC Cuts at: 0 799 1118 1275 Size: 799 319 157 Fragments arranged by size: 799 319 157 BsaHI Gr'CG_yC Cuts at: 0 4 260 1275 Size: 4 256 1015 Fragments arranged by size: 1015 256 4 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 115 138 145 168 1275 Size: 115 23 7 23 1107 Fragments arranged by size: 1107 115 23 23 7 BseYI C'CCAG_C Cuts at: 0 336 523 698 1224 1275 Size: 336 187 175 526 51 Fragments arranged by size: 526 336 187 175 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 524 1275 Size: 139 385 751 Fragments arranged by size: 751 385 139 BsiHKAI G_wGCw'C Cuts at: 0 178 422 908 1014 1275 Size: 178 244 486 106 261 Fragments arranged by size: 486 261 244 178 106 Bsp1286I G_dGCh'C Cuts at: 0 16 56 178 422 564 908 1014 1209 Size: 16 40 122 244 142 344 106 195 Cuts at: 1209 1275 Size: 66 Fragments arranged by size: 344 244 195 142 122 106 66 40 16 BsrFI r'CCGG_y Cuts at: 0 164 1195 1275 Size: 164 1031 80 Fragments arranged by size: 1031 164 80 BstAPI GCAn_nnn'nTGC Cuts at: 0 708 1275 Size: 708 567 BstXI CCAn_nnnn'nTGG Cuts at: 0 1217 1275 Size: 1217 58 BstYI r'GATC_y Cuts at: 0 548 683 885 1152 1275 Size: 548 135 202 267 123 Fragments arranged by size: 548 267 202 135 123 BtgI C'CryG_G Cuts at: 0 854 1077 1275 Size: 854 223 198 Fragments arranged by size: 854 223 198 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 801 836 1275 Size: 801 35 439 Fragments arranged by size: 801 439 35 DraIII CAC_nnn'GTG Cuts at: 0 216 1275 Size: 216 1059 DrdI GACnn_nn'nnGTC Cuts at: 0 43 1275 Size: 43 1232 EaeI y'GGCC_r Cuts at: 0 857 1193 1275 Size: 857 336 82 Fragments arranged by size: 857 336 82 EarI CTCTTCn'nnn_ Cuts at: 0 1240 1246 1275 Size: 1240 6 29 Fragments arranged by size: 1240 29 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 436 466 535 1120 1177 1275 Size: 154 282 30 69 585 57 98 Fragments arranged by size: 585 282 154 98 69 57 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 880 1237 1275 Size: 880 357 38 Fragments arranged by size: 880 357 38 EcoO109I rG'GnC_Cy Cuts at: 0 560 981 1206 1275 Size: 560 421 225 69 Fragments arranged by size: 560 421 225 69 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 750 1275 Size: 750 525 FspI TGC'GCA Cuts at: 0 712 1275 Size: 712 563 FspAI rTGC'GCAy Cuts at: 0 712 1275 Size: 712 563 HaeII r_GCGC'y Cuts at: 0 263 1275 Size: 263 1012 KasI G'GCGC_C Cuts at: 0 259 1275 Size: 259 1016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 599 1275 Size: 599 676 MslI CAynn'nnrTG Cuts at: 0 187 1275 Size: 187 1088 MspA1I CmG'CkG Cuts at: 0 523 582 1275 Size: 523 59 693 Fragments arranged by size: 693 523 59 NaeI GCC'GGC Cuts at: 0 166 1197 1275 Size: 166 1031 78 Fragments arranged by size: 1031 166 78 NarI GG'CG_CC Cuts at: 0 260 1275 Size: 260 1015 NgoMIV G'CCGG_C Cuts at: 0 164 1195 1275 Size: 164 1031 80 Fragments arranged by size: 1031 164 80 NspI r_CATG'y Cuts at: 0 400 1275 Size: 400 875 PasI CC'CwG_GG Cuts at: 0 1095 1275 Size: 1095 180 PflMI CCAn_nnn'nTGG Cuts at: 0 647 1275 Size: 647 628 PfoI T'CCnGG_A Cuts at: 0 551 1275 Size: 551 724 PspOMI G'GGCC_C Cuts at: 0 560 1205 1275 Size: 560 645 70 Fragments arranged by size: 645 560 70 PspXI vC'TCGA_Gb Cuts at: 0 1171 1275 Size: 1171 104 PvuII CAG'CTG Cuts at: 0 523 582 1275 Size: 523 59 693 Fragments arranged by size: 693 523 59 RsrII CG'GwC_CG Cuts at: 0 99 1275 Size: 99 1176 SfoI GGC'GCC Cuts at: 0 261 1275 Size: 261 1014 SmaI CCC'GGG Cuts at: 0 109 1275 Size: 109 1166 SmlI C'TyrA_G Cuts at: 0 1171 1275 Size: 1171 104 StuI AGG'CCT Cuts at: 0 391 1169 1243 1275 Size: 391 778 74 32 Fragments arranged by size: 778 391 74 32 StyI C'CwwG_G Cuts at: 0 641 725 1275 Size: 641 84 550 Fragments arranged by size: 641 550 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 116 498 1275 Size: 116 382 777 Fragments arranged by size: 777 382 116 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 138 170 533 565 1275 Size: 138 32 363 32 710 Fragments arranged by size: 710 363 138 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 586 861 1275 Size: 586 275 414 Fragments arranged by size: 586 414 275 XhoI C'TCGA_G Cuts at: 0 1171 1275 Size: 1171 104 XmaI C'CCGG_G Cuts at: 0 107 1275 Size: 107 1168 Enzymes that do cut: AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI BglII BlpI Bme1580I BpmI Bpu10I BsaBI BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BsrFI BstAPI BstXI BstYI BtgI CspCI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII KasI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI PspOMI PspXI PvuII RsrII SfoI SmaI SmlI StuI StyI TaqII TstI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI BfrBI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI DraI EagI EciI EcoICRI EcoRI EcoRV FseI Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XmnI ZraI