(Circular) (Six-base) MAPSORT of: pFN21AB5024.seq Check: 8446 from: 1 to: 5989 pFN21AB5024 5989 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3274 3274 Size: 5989 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3799 278 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3332 1937 Size: 1395 4594 Acc65I G'GTAC_C Cuts at: 3310 3310 Size: 5989 AclI AA'CG_TT Cuts at: 1563 4116 4489 1563 Size: 2553 373 3063 Fragments arranged by size: 3063 2553 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2665 4052 5436 5704 1632 Size: 1033 1387 1384 268 1917 Fragments arranged by size: 1917 1387 1384 1033 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5792 AflIII A'CryG_T Cuts at: 1203 2085 2472 4888 5865 1203 Size: 882 387 2416 977 1327 Fragments arranged by size: 2416 1327 977 882 387 AgeI A'CCGG_T Cuts at: 4820 4820 Size: 5989 AhdI GACnn_n'nnGTC Cuts at: 4718 4718 Size: 5989 AleI CACnn'nnGTG Cuts at: 1166 3236 3278 1166 Size: 2070 42 3877 Fragments arranged by size: 3877 2070 42 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2553 1184 Size: 1369 4620 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2603 2635 2603 Size: 32 5957 AlwNI CAG_nnn'CTG Cuts at: 2278 2323 2423 2951 3017 3732 5304 2278 Size: 45 100 528 66 715 1572 2963 Fragments arranged by size: 2963 1572 715 528 100 66 45 ApaI G_GGCC'C Cuts at: 2031 2743 2031 Size: 712 5277 ApaLI G'TGCA_C Cuts at: 4046 5202 4046 Size: 1156 4833 ApoI r'AATT_y Cuts at: 1493 3298 3524 3676 5785 1493 Size: 1805 226 152 2109 1697 Fragments arranged by size: 2109 1805 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4543 160 Size: 872 3511 1606 Fragments arranged by size: 3511 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 AvaI C'yCGr_G Cuts at: 1870 1943 2348 3314 1870 Size: 73 405 966 4545 Fragments arranged by size: 4545 966 405 73 BamHI G'GATC_C Cuts at: 3319 3686 3319 Size: 367 5622 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3310 4765 618 Size: 325 217 627 1523 1455 1842 Fragments arranged by size: 1842 1523 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2031 2501 2743 2850 3308 1391 Size: 640 470 242 107 458 4072 Fragments arranged by size: 4072 640 470 458 242 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5018 BbvCI CC'TCA_GC Cuts at: 2453 2709 2453 Size: 256 5733 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3194 3228 4180 4214 1903 Size: 34 1257 34 952 34 3678 Fragments arranged by size: 3678 1257 952 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3881 5091 3881 Size: 1210 4779 BclI T'GATC_A Cuts at: 1548 3261 1548 Size: 1713 4276 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2439 2720 2816 Size: 107 122 71 631 605 767 281 96 Cuts at: 2816 3074 4600 136 Size: 258 1526 1525 Fragments arranged by size: 1526 1525 767 631 605 281 258 122 107 96 71 BglII A'GATC_T Cuts at: 5984 5984 Size: 5989 BlpI GC'TnA_GC Cuts at: 3018 3406 3018 Size: 388 5601 Bme1580I G_kGCm'C Cuts at: 1790 2031 2743 4050 5206 1790 Size: 241 712 1307 1156 2573 Fragments arranged by size: 2573 1307 1156 712 241 BmgBI CAC'GTC Cuts at: 1318 2473 1318 Size: 1155 4834 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2710 3720 4678 Size: 416 53 83 399 24 1293 1010 958 Cuts at: 4678 442 Size: 1753 Fragments arranged by size: 1753 1293 1010 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 4649 1239 Size: 1106 2304 2579 Fragments arranged by size: 2579 2304 1106 Bpu10I CC'TnA_GC Cuts at: 2453 2709 2453 Size: 256 5733 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3017 4114 4979 5277 3017 Size: 1097 865 298 3729 Fragments arranged by size: 3729 1097 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4652 882 Size: 617 3153 2219 Fragments arranged by size: 3153 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2086 3780 493 Size: 711 882 1694 2702 Fragments arranged by size: 2702 1694 882 711 BsaBI GATnn'nnATC Cuts at: 5983 5983 Size: 5989 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3796 4178 4813 275 Size: 53 83 186 936 2263 382 635 1451 Fragments arranged by size: 2263 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4422 4820 5094 5241 5652 4422 Size: 398 274 147 411 4759 Fragments arranged by size: 4759 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5764 Fragments arranged by size: 5764 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2702 1186 Size: 1516 4473 BseYI C'CCAG_C Cuts at: 2097 5192 5688 2097 Size: 3095 496 2398 Fragments arranged by size: 3095 2398 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2312 2549 2810 3302 1163 Size: 573 576 237 261 492 3850 Fragments arranged by size: 3850 576 573 492 261 237 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2190 3439 4200 5228 1540 Size: 275 186 189 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 275 189 186 BsiHKAI G_wGCw'C Cuts at: 2501 2850 4050 4135 5206 2501 Size: 349 1200 85 1071 3284 Fragments arranged by size: 3284 1200 1071 349 85 BsiWI C'GTAC_G Cuts at: 2834 2834 Size: 5989 BsmI GAATG_Cn' Cuts at: 3500 3593 3500 Size: 93 5896 BsmBI CGTCTCn'nnnn_ Cuts at: 2177 2177 Size: 5989 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2501 2743 2850 3308 4050 4135 Size: 399 241 470 242 107 458 742 85 Cuts at: 4135 5206 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 470 458 399 242 241 107 85 BspEI T'CCGG_A Cuts at: 5652 5652 Size: 5989 BspHI T'CATG_A Cuts at: 2235 3878 5608 2235 Size: 1643 1730 2616 Fragments arranged by size: 2616 1730 1643 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2917 3274 3344 844 Size: 2073 357 70 3489 Fragments arranged by size: 3489 2073 357 70 BsrBI CCG'CTC Cuts at: 1394 1823 3022 3876 1394 Size: 429 1199 854 3507 Fragments arranged by size: 3507 1199 854 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4484 4658 65 Size: 1183 3236 174 1396 Fragments arranged by size: 3236 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2865 4633 4820 1700 Size: 110 1055 1768 187 2869 Fragments arranged by size: 2869 1768 1055 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5989 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5989 BssSI C'ACGA_G Cuts at: 4049 5061 4049 Size: 1012 4977 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5989 BstBI TT'CG_AA Cuts at: 4793 4793 Size: 5989 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5989 BstYI r'GATC_y Cuts at: 1972 2448 2757 2826 2958 3319 3686 4071 4088 Size: 476 309 69 132 361 367 385 17 Cuts at: 4088 5540 5626 5638 5984 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 476 385 367 361 346 309 132 86 69 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2259 2523 2811 513 Size: 552 939 255 264 288 3691 Fragments arranged by size: 3691 939 552 288 264 255 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2836 3014 487 Size: 683 56 1610 178 3462 Fragments arranged by size: 3462 1610 683 178 56 BtsI GCAGTG_nn' Cuts at: 3505 4299 4319 3505 Size: 794 20 5175 Fragments arranged by size: 5175 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5989 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5954 DraI TTT'AAA Cuts at: 3293 3293 Size: 5989 DraIII CAC_nnn'GTG Cuts at: 2023 2023 Size: 5989 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4996 809 Size: 507 3680 1802 Fragments arranged by size: 3680 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2187 2256 2431 Size: 54 1425 50 275 18 357 69 175 Cuts at: 2431 2553 3201 3436 4325 5954 8 Size: 122 648 235 889 1629 43 Fragments arranged by size: 1629 1425 889 648 357 275 235 175 122 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2187 3436 1537 Size: 275 375 1249 4090 Fragments arranged by size: 4090 1249 375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2763 3919 1353 Size: 1410 1156 3423 Fragments arranged by size: 3423 1410 1156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2993 4576 4950 5096 387 Size: 1064 138 1404 1583 374 146 1280 Fragments arranged by size: 1583 1404 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2499 2848 2499 Size: 349 5640 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2665 4052 4649 5436 5704 1239 Size: 393 713 320 1387 597 787 268 1524 Fragments arranged by size: 1524 1387 787 713 597 393 320 268 EcoO109I rG'GnC_Cy Cuts at: 2027 2352 2739 2929 3091 2027 Size: 325 387 190 162 4925 Fragments arranged by size: 4925 387 325 190 162 EcoRI G'AATT_C Cuts at: 3298 3676 3298 Size: 378 5611 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2674 2695 3094 2674 Size: 21 399 5569 Fragments arranged by size: 5569 399 21 FspI TGC'GCA Cuts at: 4495 4495 Size: 5989 HaeII r_GCGC'y Cuts at: 1678 2042 5136 1678 Size: 364 3094 2531 Fragments arranged by size: 3094 2531 364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4630 4662 4704 4736 1373 Size: 32 135 32 3058 32 42 32 2626 Fragments arranged by size: 3058 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2695 3333 3579 669 Size: 988 281 757 638 246 3079 Fragments arranged by size: 3079 988 757 638 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5989 HpaI GTT'AAC Cuts at: 2695 3579 2695 Size: 884 5105 KpnI G_GTAC'C Cuts at: 3314 3314 Size: 5989 MfeI C'AATT_G Cuts at: 2011 3588 2011 Size: 1577 4412 MluI A'CGCG_T Cuts at: 5865 5865 Size: 5989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 2148 5103 5287 1305 Size: 789 54 2955 184 2007 Fragments arranged by size: 2955 2007 789 184 54 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4167 Fragments arranged by size: 4167 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2549 3236 3278 3947 4306 4465 518 Size: 648 1383 687 42 669 359 159 2042 Fragments arranged by size: 2042 1383 687 669 648 359 159 42 MspA1I CmG'CkG Cuts at: 1606 2169 2708 3405 4082 5230 5475 1606 Size: 563 539 697 677 1148 245 2120 Fragments arranged by size: 2120 1148 697 677 563 539 245 NaeI GCC'GGC Cuts at: 2867 2867 Size: 5989 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4498 Fragments arranged by size: 4498 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5989 NgoMIV G'CCGG_C Cuts at: 2865 2865 Size: 5989 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5989 NotI GC'GGCC_GC Cuts at: 3436 3436 Size: 5989 NspI r_CATG'y Cuts at: 1132 1229 3227 3347 4892 1132 Size: 97 1998 120 1545 2229 Fragments arranged by size: 2229 1998 1545 120 97 PasI CC'CwG_GG Cuts at: 1348 2503 1348 Size: 1155 4834 PciI A'CATG_T Cuts at: 4888 4888 Size: 5989 PflMI CCAn_nnn'nTGG Cuts at: 1108 2023 1108 Size: 915 5074 PfoI T'CCnGG_A Cuts at: 2580 2580 Size: 5989 PmeI GTTT'AAAC Cuts at: 3293 3293 Size: 5989 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4058 4090 5571 5603 4058 Size: 32 1481 32 4444 Fragments arranged by size: 4444 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2352 2929 2352 Size: 577 5412 PshAI GACnn'nnGTC Cuts at: 1613 2216 1613 Size: 603 5386 PsiI TTA'TAA Cuts at: 3559 3559 Size: 5989 PspOMI G'GGCC_C Cuts at: 2027 2739 2027 Size: 712 5277 PstI C_TGCA'G Cuts at: 830 2319 2736 3341 830 Size: 1489 417 605 3478 Fragments arranged by size: 3478 1489 605 417 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5989 PvuII CAG'CTG Cuts at: 2708 2708 Size: 5989 SacI G_AGCT'C Cuts at: 2501 2850 2501 Size: 349 5640 SalI G'TCGA_C Cuts at: 1936 3331 1936 Size: 1395 4594 SanDI GG'GwC_CC Cuts at: 2352 2352 Size: 5989 SbfI CC_TGCA'GG Cuts at: 3341 3341 Size: 5989 ScaI AGT'ACT Cuts at: 4237 5949 4237 Size: 1712 4277 SfcI C'TryA_G Cuts at: 826 1045 2315 2732 2856 3337 4472 5153 5344 Size: 219 1270 417 124 481 1135 681 191 Cuts at: 5344 826 Size: 1471 Fragments arranged by size: 1471 1270 1135 681 481 417 219 191 124 SfiI GGCCn_nnn'nGGCC Cuts at: 2720 2720 Size: 5989 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5989 SmaI CCC'GGG Cuts at: 1872 3316 1872 Size: 1444 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3032 4093 4994 5256 820 Size: 197 926 1089 1061 901 262 1553 Fragments arranged by size: 1553 1089 1061 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3780 493 Size: 3287 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5989 SphI G_CATG'C Cuts at: 1132 3227 3347 1132 Size: 2095 120 3774 Fragments arranged by size: 3774 2095 120 SspI AAT'ATT Cuts at: 5 52 3913 5 Size: 47 3861 2081 Fragments arranged by size: 3861 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5989 StyI C'CwwG_G Cuts at: 513 1065 2004 3078 3132 3428 513 Size: 552 939 1074 54 296 3074 Fragments arranged by size: 3074 1074 939 552 296 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4016 4033 4186 4371 4816 325 Size: 1255 2436 17 153 185 445 1498 Fragments arranged by size: 2436 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4235 5947 96 Size: 274 80 33 51 1443 2258 1712 138 Fragments arranged by size: 2258 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2576 2608 2811 2843 5949 5981 2576 Size: 32 203 32 3106 32 2584 Fragments arranged by size: 3106 2584 203 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5989 XbaI T'CTAG_A Cuts at: 3325 3325 Size: 5989 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 3076 2038 Size: 1038 4951 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5989 XmaI C'CCGG_G Cuts at: 1870 3314 1870 Size: 1444 4545 XmnI GAAnn'nnTTC Cuts at: 4118 4118 Size: 5989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3797 276 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI