################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FauI FseI FspI HaeII HhaI Hin1I HinP1I HindIII KasI KpnI KspI MaeII MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SapI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Tsp45I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Alw44I 1670 GTGCAC G^TGCAC ApaI 1378 GGGCCC GGGCC^C ApaLI 1670 GTGCAC G^TGCAC AseI 1164 ATTAAT AT^TAAT AsnI 1164 ATTAAT AT^TAAT AspHI 1670 GTGCAC G[AT]GC[AT]^C BamHI 1587 GGATCC G^GATCC BbsI 509 GAAGAC GAAGAC(2/6) BglI 1416 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 38 GCTCAGC GC^T[ACGT]AGC Bpu1102I 38 GCTCAGC GC^T[ACGT]AGC BpuAI 509 GAAGAC GAAGAC(2/6) BsaBI 205 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 713 GAGACC GGTCTC(1/5) BsiHKAI 1670 GTGCAC G[AT]GC[AT]^C BsmAI 713 GAGAC GTCTC(1/5) BspHI 566 TCATGA T^CATGA BstBI 1025 TTCGAA TT^CGAA CelII 38 GCTCAGC GC^T[ACGT]AGC DraIII 1112 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1061 GACCTGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 159 GAATTC G^AATTC HgaI 85 GACGC GACGC(5/10) MamI 205 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 1508 CATATG CA^TATG NheI 577 GCTAGC G^CTAGC NspI 996 ACATGC [GA]CATG^[CT] NspV 1025 TTCGAA TT^CGAA PleI 247 GACTC GAGTC(4/5) PshAI 985 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1164 ATTAAT AT^TAAT RcaI 566 TCATGA T^CATGA SbfI 1663 CCTGCAGG CCTGCA^GG ScaI 1760 AGTACT AGT^ACT SfiI 1415 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 1025 TTCGAA TT^CGAA Sse8387I 1663 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 1369 GTAC GT^AC 1761 GTAC BclI 765 TGATCA T^GATCA 887 TGATCA BfaI 578 CTAG C^TAG 1951 CTAG BglII 289 AGATCT A^GATCT 362 AGATCT BsmI 1895 GAATGC GAATGC(1/-1) 920 GCATTC BspMI 1666 GCAGGT ACCTGC(4/8) 1925 GCAGGT BstAPI 1657 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1783 GCAGTTCCTGC BstXI 737 CCAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1206 CCAGCAGTCTGG EcoT22I 274 ATGCAT ATGCA^T 1532 ATGCAT FbaI 765 TGATCA T^GATCA 887 TGATCA HpaI 79 GTTAAC GTT^AAC 444 GTTAAC MaeI 578 CTAG C^TAG 1951 CTAG MaeIII 264 GTTAC ^GT[ACGT]AC 483 GTTAC MslI 532 CATGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1916 CACCTCAGTG MspA1I 41 CAGCTG C[AC]G^C[GT]G 1724 CAGCTG NsiI 274 ATGCAT ATGCA^T 1532 ATGCAT PpuMI 433 AGGTCCT [GA]G^G[AT]CC[CT] 975 AGGACCT PvuII 41 CAGCTG CAG^CTG 1724 CAGCTG RsaI 1369 GTAC GT^AC 1761 GTAC SexAI 1062 ACCTGGT A^CC[AT]GGT 1113 ACCTGGT TaqI 1026 TCGA T^CGA 1071 TCGA TthHB8I 1026 TCGA T^CGA 1071 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI Van91I XcmI XhoII