(Circular) (Six-base) MAPSORT of: pFN21AB7892.seq Check: 9091 from: 1 to: 6664 pFN21AB7892 6664 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3662 3662 Size: 6664 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4474 278 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4007 1937 Size: 2070 4594 Acc65I G'GTAC_C Cuts at: 3985 3985 Size: 6664 AclI AA'CG_TT Cuts at: 1563 4791 5164 1563 Size: 3228 373 3063 Fragments arranged by size: 3228 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2618 3221 4727 6111 6379 1632 Size: 986 603 1506 1384 268 1917 Fragments arranged by size: 1917 1506 1384 986 603 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6467 AflIII A'CryG_T Cuts at: 1203 5563 6540 1203 Size: 4360 977 1327 Fragments arranged by size: 4360 1327 977 AgeI A'CCGG_T Cuts at: 5495 5495 Size: 6664 AhdI GACnn_n'nnGTC Cuts at: 5393 5393 Size: 6664 AleI CACnn'nnGTG Cuts at: 1166 3925 1166 Size: 2759 3905 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3237 3943 1184 Size: 2053 706 3905 Fragments arranged by size: 3905 2053 706 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2544 2576 2544 Size: 32 6632 AlwNI CAG_nnn'CTG Cuts at: 2525 3431 3668 3794 4407 5979 2525 Size: 906 237 126 613 1572 3210 Fragments arranged by size: 3210 1572 906 613 237 126 ApaI G_GGCC'C Cuts at: 3387 3387 Size: 6664 ApaLI G'TGCA_C Cuts at: 3675 4721 5877 3675 Size: 1046 1156 4462 Fragments arranged by size: 4462 1156 1046 ApoI r'AATT_y Cuts at: 1493 2164 3134 3598 3973 4199 4351 6460 1493 Size: 671 970 464 375 226 152 2109 1697 Fragments arranged by size: 2109 1697 970 671 464 375 226 152 AseI AT'TA_AT Cuts at: 160 1032 3170 5218 160 Size: 872 2138 2048 1606 Fragments arranged by size: 2138 2048 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 AvaI C'yCGr_G Cuts at: 1870 1943 3989 1870 Size: 73 2046 4545 Fragments arranged by size: 4545 2046 73 BamHI G'GATC_C Cuts at: 3592 3994 4361 3592 Size: 402 367 5895 Fragments arranged by size: 5895 402 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3985 5440 618 Size: 325 217 627 2198 1455 1842 Fragments arranged by size: 2198 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2119 3387 3811 3983 1391 Size: 728 1268 424 172 4072 Fragments arranged by size: 4072 1268 728 424 172 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2521 928 Size: 971 622 5071 Fragments arranged by size: 5071 971 622 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4855 4889 1903 Size: 34 2918 34 3678 Fragments arranged by size: 3678 2918 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4556 5766 4556 Size: 1210 5454 BclI T'GATC_A Cuts at: 1548 2770 2892 1548 Size: 1222 122 5320 Fragments arranged by size: 5320 1222 122 BfrBI ATG'CAT Cuts at: 2281 3539 2281 Size: 1258 5406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3427 5275 136 Size: 107 122 71 631 605 1755 1848 1525 Fragments arranged by size: 1848 1755 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2294 2367 6659 2294 Size: 73 4292 2299 Fragments arranged by size: 4292 2299 73 BlpI GC'TnA_GC Cuts at: 2044 4081 2044 Size: 2037 4627 Bme1580I G_kGCm'C Cuts at: 1790 3387 3679 4725 5881 1790 Size: 1597 292 1046 1156 2573 Fragments arranged by size: 2573 1597 1156 1046 292 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6664 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3165 3344 3813 Size: 416 53 83 399 24 1748 179 469 Cuts at: 3813 4395 5353 442 Size: 582 958 1753 Fragments arranged by size: 1753 1748 958 582 469 416 399 179 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2586 1056 Size: 1530 5134 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3264 3264 Size: 6664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2667 2970 3408 3489 3798 3900 5324 1239 Size: 1428 303 438 81 309 102 1424 2579 Fragments arranged by size: 2579 1428 1424 438 309 303 102 81 Bpu10I CC'TnA_GC Cuts at: 3579 3579 Size: 6664 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4789 5654 5952 4789 Size: 865 298 5501 Fragments arranged by size: 5501 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2712 5327 882 Size: 617 1213 2615 2219 Fragments arranged by size: 2615 2219 1213 617 BsaAI yAC'GTr Cuts at: 493 1204 4455 493 Size: 711 3251 2702 Fragments arranged by size: 3251 2702 711 BsaBI GATnn'nnATC Cuts at: 2214 6658 2214 Size: 4444 2220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4471 4853 5488 275 Size: 53 83 186 936 2938 382 635 1451 Fragments arranged by size: 2938 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5097 5495 5769 5916 6327 5097 Size: 398 274 147 411 5434 Fragments arranged by size: 5434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2011 2041 2968 2998 3793 Size: 30 165 30 608 30 927 30 795 Cuts at: 3793 3823 1178 Size: 30 4019 Fragments arranged by size: 4019 927 795 608 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2094 2232 2370 3483 3555 3801 1186 Size: 908 138 138 1113 72 246 4049 Fragments arranged by size: 4049 1113 908 246 138 138 72 BseYI C'CCAG_C Cuts at: 3727 5867 6363 3727 Size: 2140 496 4028 Fragments arranged by size: 4028 2140 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3444 3858 3949 1163 Size: 573 1708 414 91 3878 Fragments arranged by size: 3878 1708 573 414 91 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4114 4875 5903 1540 Size: 275 186 2113 761 1028 2301 Fragments arranged by size: 2301 2113 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3679 4725 4810 5881 3679 Size: 1046 85 1071 4462 Fragments arranged by size: 4462 1071 1046 85 BsmI GAATG_Cn' Cuts at: 2925 3906 4175 4268 2925 Size: 981 269 93 5321 Fragments arranged by size: 5321 981 269 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2119 3387 3679 3811 3983 4725 4810 Size: 399 329 1268 292 132 172 742 85 Cuts at: 4810 5881 1391 Size: 1071 2174 Fragments arranged by size: 2174 1268 1071 742 399 329 292 172 132 85 BspEI T'CCGG_A Cuts at: 6327 6327 Size: 6664 BspHI T'CATG_A Cuts at: 2571 4553 6283 2571 Size: 1982 1730 2952 Fragments arranged by size: 2952 1982 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3662 3921 4019 844 Size: 2818 259 98 3489 Fragments arranged by size: 3489 2818 259 98 BsrBI CCG'CTC Cuts at: 1394 1823 4551 1394 Size: 429 2728 3507 Fragments arranged by size: 3507 2728 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5159 5333 65 Size: 1183 3911 174 1396 Fragments arranged by size: 3911 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5308 5495 1700 Size: 110 3498 187 2869 Fragments arranged by size: 3498 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6664 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6664 BssSI C'ACGA_G Cuts at: 4724 5736 4724 Size: 1012 5652 BstAPI GCAn_nnn'nTGC Cuts at: 794 3668 3794 794 Size: 2874 126 3664 Fragments arranged by size: 3664 2874 126 BstBI TT'CG_AA Cuts at: 3031 5468 3031 Size: 2437 4227 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6664 BstXI CCAn_nnnn'nTGG Cuts at: 2749 3218 2749 Size: 469 6195 BstYI r'GATC_y Cuts at: 1972 2294 2367 3262 3592 3994 4361 4746 4763 Size: 322 73 895 330 402 367 385 17 Cuts at: 4763 6215 6301 6313 6659 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 895 402 385 367 346 330 322 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3379 3388 3406 513 Size: 552 939 1375 9 18 3771 Fragments arranged by size: 3771 1375 939 552 18 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2556 487 Size: 683 56 1330 4595 Fragments arranged by size: 4595 1330 683 56 BtsI GCAGTG_nn' Cuts at: 2105 4180 4974 4994 2105 Size: 2075 794 20 3775 Fragments arranged by size: 3775 2075 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6664 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6629 DraI TTT'AAA Cuts at: 3968 3968 Size: 6664 DraIII CAC_nnn'GTG Cuts at: 3122 3122 Size: 6664 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3072 5671 809 Size: 507 1756 2599 1802 Fragments arranged by size: 2599 1802 1756 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4111 5000 6629 Size: 54 1425 50 275 18 2281 889 1629 Cuts at: 6629 8 Size: 43 Fragments arranged by size: 2281 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4111 1537 Size: 275 2299 4090 Fragments arranged by size: 4090 2299 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2041 3076 3756 4594 1353 Size: 688 1035 680 838 3423 Fragments arranged by size: 3423 1035 838 688 680 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5251 5625 5771 387 Size: 1064 138 3662 374 146 1280 Fragments arranged by size: 3662 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2618 2667 2970 3221 3408 3489 3798 Size: 393 986 49 303 251 187 81 309 Cuts at: 3798 3900 4727 5324 6111 6379 1239 Size: 102 827 597 787 268 1524 Fragments arranged by size: 1524 986 827 787 597 393 309 303 268 251 187 102 81 49 EcoO109I rG'GnC_Cy Cuts at: 2439 2981 3384 3401 3428 2439 Size: 542 403 17 27 5675 Fragments arranged by size: 5675 542 403 27 17 EcoRI G'AATT_C Cuts at: 2164 3973 4351 2164 Size: 1809 378 4477 Fragments arranged by size: 4477 1809 378 FspI TGC'GCA Cuts at: 5170 5170 Size: 6664 HaeII r_GCGC'y Cuts at: 1678 5811 1678 Size: 4133 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2792 2824 2877 2909 3793 Size: 32 135 32 1220 32 53 32 884 Cuts at: 3793 3825 5305 5337 5379 5411 1373 Size: 32 1480 32 42 32 2626 Fragments arranged by size: 2626 1480 1220 884 135 53 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2086 2451 2999 4008 4254 669 Size: 988 281 148 365 548 1009 246 3079 Fragments arranged by size: 3079 1009 988 548 365 281 246 148 HindIII A'AGCT_T Cuts at: 748 748 Size: 6664 HpaI GTT'AAC Cuts at: 2086 2451 4254 2086 Size: 365 1803 4496 Fragments arranged by size: 4496 1803 365 KpnI G_GTAC'C Cuts at: 3989 3989 Size: 6664 MfeI C'AATT_G Cuts at: 4263 4263 Size: 6664 MluI A'CGCG_T Cuts at: 6540 6540 Size: 6664 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2317 3824 5778 5962 1305 Size: 1012 1507 1954 184 2007 Fragments arranged by size: 2007 1954 1507 1012 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4842 Fragments arranged by size: 4842 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2541 3925 4622 4981 5140 518 Size: 648 1375 1384 697 359 159 2042 Fragments arranged by size: 2042 1384 1375 697 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2048 3731 4080 4757 5905 6150 1606 Size: 442 1683 349 677 1148 245 2120 Fragments arranged by size: 2120 1683 1148 677 442 349 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3379 3388 3406 513 Size: 552 939 1375 9 18 3771 Fragments arranged by size: 3771 1375 939 552 18 9 NdeI CA'TA_TG Cuts at: 387 3514 387 Size: 3127 3537 NheI G'CTAG_C Cuts at: 1052 2582 1052 Size: 1530 5134 NotI GC'GGCC_GC Cuts at: 4111 4111 Size: 6664 NsiI A_TGCA'T Cuts at: 2283 3541 2283 Size: 1258 5406 NspI r_CATG'y Cuts at: 1132 1229 3005 4022 5567 1132 Size: 97 1776 1017 1545 2229 Fragments arranged by size: 2229 1776 1545 1017 97 PasI CC'CwG_GG Cuts at: 1348 3523 3895 1348 Size: 2175 372 4117 Fragments arranged by size: 4117 2175 372 PciI A'CATG_T Cuts at: 5563 5563 Size: 6664 PflMI CCAn_nnn'nTGG Cuts at: 1108 2525 2945 3122 3431 3756 1108 Size: 1417 420 177 309 325 4016 Fragments arranged by size: 4016 1417 420 325 309 177 PfoI T'CCnGG_A Cuts at: 3472 3472 Size: 6664 PmeI GTTT'AAAC Cuts at: 3968 3968 Size: 6664 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4733 4765 6246 6278 4733 Size: 32 1481 32 5119 Fragments arranged by size: 5119 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2439 2981 2439 Size: 542 6122 PshAI GACnn'nnGTC Cuts at: 1613 2994 1613 Size: 1381 5283 PsiI TTA'TAA Cuts at: 3468 4234 3468 Size: 766 5898 PspOMI G'GGCC_C Cuts at: 3383 3383 Size: 6664 PstI C_TGCA'G Cuts at: 830 2112 3010 3673 4016 830 Size: 1282 898 663 343 3478 Fragments arranged by size: 3478 1282 898 663 343 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6664 PvuII CAG'CTG Cuts at: 2048 3731 2048 Size: 1683 4981 SalI G'TCGA_C Cuts at: 1936 4006 1936 Size: 2070 4594 SbfI CC_TGCA'GG Cuts at: 3673 4016 3673 Size: 343 6321 ScaI AGT'ACT Cuts at: 3767 4912 6624 3767 Size: 1145 1712 3807 Fragments arranged by size: 3807 1712 1145 SexAI A'CCwGG_T Cuts at: 3067 3118 3067 Size: 51 6613 SfcI C'TryA_G Cuts at: 826 1045 2108 3006 3128 3669 4012 5147 5828 Size: 219 1063 898 122 541 343 1135 681 Cuts at: 5828 6019 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1063 898 681 541 343 219 191 122 SfiI GGCCn_nnn'nGGCC Cuts at: 3427 3427 Size: 6664 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6664 SmaI CCC'GGG Cuts at: 1872 3991 1872 Size: 2119 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4768 5669 5931 820 Size: 197 926 2825 901 262 1553 Fragments arranged by size: 2825 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4455 493 Size: 3962 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6664 SphI G_CATG'C Cuts at: 1132 4022 1132 Size: 2890 3774 SspI AAT'ATT Cuts at: 5 52 4588 5 Size: 47 4536 2081 Fragments arranged by size: 4536 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6664 StyI C'CwwG_G Cuts at: 513 1065 2004 2742 3379 3388 3406 3750 4103 Size: 552 939 738 637 9 18 344 353 Cuts at: 4103 513 Size: 3074 Fragments arranged by size: 3074 939 738 637 552 353 344 18 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3006 4691 4708 4861 5046 5491 325 Size: 1255 1426 1685 17 153 185 445 1498 Fragments arranged by size: 1685 1498 1426 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3765 4910 6622 Size: 274 80 33 51 1443 1788 1145 1712 Cuts at: 6622 96 Size: 138 Fragments arranged by size: 1788 1712 1443 1145 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6624 6656 6624 Size: 32 6632 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6664 XbaI T'CTAG_A Cuts at: 4000 4000 Size: 6664 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2540 3386 3413 2540 Size: 846 27 5791 Fragments arranged by size: 5791 846 27 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6664 XmaI C'CCGG_G Cuts at: 1870 3989 1870 Size: 2119 4545 XmnI GAAnn'nnTTC Cuts at: 4793 4793 Size: 6664 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4472 276 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfoI SrfI SwaI