(Circular) (Six-base) MAPSORT of: pFN21AE0693.seq Check: 7980 from: 1 to: 5551 pFN21AE0693 5551 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2704 3361 278 Size: 53 83 186 936 1168 657 2468 Fragments arranged by size: 2468 1168 936 657 186 83 53 AccI GT'mk_AC Cuts at: 1937 2894 1937 Size: 957 4594 Acc65I G'GTAC_C Cuts at: 2872 2872 Size: 5551 AclI AA'CG_TT Cuts at: 1563 3678 4051 1563 Size: 2115 373 3063 Fragments arranged by size: 3063 2115 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3614 4998 5266 1632 Size: 1982 1384 268 1917 Fragments arranged by size: 1982 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5354 AflIII A'CryG_T Cuts at: 1203 4450 5427 1203 Size: 3247 977 1327 Fragments arranged by size: 3247 1327 977 AgeI A'CCGG_T Cuts at: 4382 4382 Size: 5551 AhdI GACnn_n'nnGTC Cuts at: 4280 4280 Size: 5551 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5551 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5551 AlwNI CAG_nnn'CTG Cuts at: 3294 4866 3294 Size: 1572 3979 ApaLI G'TGCA_C Cuts at: 3608 4764 3608 Size: 1156 4395 ApoI r'AATT_y Cuts at: 1493 2571 2860 3086 3238 5347 1493 Size: 1078 289 226 152 2109 1697 Fragments arranged by size: 2109 1697 1078 289 226 152 AseI AT'TA_AT Cuts at: 160 1032 4105 160 Size: 872 3073 1606 Fragments arranged by size: 3073 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 AvaI C'yCGr_G Cuts at: 1870 1943 2481 2876 1870 Size: 73 538 395 4545 Fragments arranged by size: 4545 538 395 73 BamHI G'GATC_C Cuts at: 2881 3248 2881 Size: 367 5184 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2872 4327 618 Size: 325 217 627 1085 1455 1842 Fragments arranged by size: 1842 1455 1085 627 325 217 BanII G_rGCy'C Cuts at: 1391 2870 1391 Size: 1479 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2329 2760 2782 928 Size: 971 430 431 22 3697 Fragments arranged by size: 3697 971 431 430 22 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3742 3776 1903 Size: 34 1805 34 3678 Fragments arranged by size: 3678 1805 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3443 4653 3443 Size: 1210 4341 BclI T'GATC_A Cuts at: 1548 2461 1548 Size: 913 4638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4162 136 Size: 107 122 71 631 605 2490 1525 Fragments arranged by size: 2490 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2822 5546 2822 Size: 2724 2827 BlpI GC'TnA_GC Cuts at: 2968 2968 Size: 5551 Bme1580I G_kGCm'C Cuts at: 1790 3612 4768 1790 Size: 1822 1156 2573 Fragments arranged by size: 2573 1822 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5551 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2481 3282 4240 Size: 416 53 83 399 24 1064 801 958 Cuts at: 4240 442 Size: 1753 Fragments arranged by size: 1753 1064 958 801 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5551 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2065 2211 2265 2065 Size: 146 54 5351 Fragments arranged by size: 5351 146 54 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4211 1239 Size: 2972 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3676 4541 4839 3676 Size: 865 298 4388 Fragments arranged by size: 4388 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4214 882 Size: 617 2715 2219 Fragments arranged by size: 2715 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3342 493 Size: 711 2138 2702 Fragments arranged by size: 2702 2138 711 BsaBI GATnn'nnATC Cuts at: 2161 2167 2218 2809 2815 2821 5545 2161 Size: 6 51 591 6 6 2724 2167 Fragments arranged by size: 2724 2167 591 51 6 6 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2701 3358 3740 4375 Size: 53 83 186 936 1168 657 382 635 Cuts at: 4375 275 Size: 1451 Fragments arranged by size: 1451 1168 936 657 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2316 3984 4382 4656 4803 5214 2316 Size: 1668 398 274 147 411 2653 Fragments arranged by size: 2653 1668 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2059 2087 2089 2117 1178 Size: 30 165 30 656 28 2 28 4612 Fragments arranged by size: 4612 656 165 30 30 28 28 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2679 1186 Size: 1493 4058 BseYI C'CCAG_C Cuts at: 4754 5250 4754 Size: 496 5055 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4978 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3001 3762 4790 1540 Size: 275 186 1000 761 1028 2301 Fragments arranged by size: 2301 1028 1000 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3612 3697 4768 3612 Size: 85 1071 4395 Fragments arranged by size: 4395 1071 85 BsmI GAATG_Cn' Cuts at: 3062 3155 3062 Size: 93 5458 BsmBI CGTCTCn'nnnn_ Cuts at: 2236 2236 Size: 5551 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2870 3612 3697 4768 1391 Size: 399 1080 742 85 1071 2174 Fragments arranged by size: 2174 1080 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5214 5214 Size: 5551 BspHI T'CATG_A Cuts at: 3440 5170 3440 Size: 1730 3821 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2091 2906 844 Size: 1247 815 3489 Fragments arranged by size: 3489 1247 815 BsrBI CCG'CTC Cuts at: 1394 1823 3438 1394 Size: 429 1615 3507 Fragments arranged by size: 3507 1615 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4046 4220 65 Size: 1183 2798 174 1396 Fragments arranged by size: 2798 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2277 4195 4382 1700 Size: 110 467 1918 187 2869 Fragments arranged by size: 2869 1918 467 187 110 BsrGI T'GTAC_A Cuts at: 96 2388 96 Size: 2292 3259 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5551 BssSI C'ACGA_G Cuts at: 2082 3611 4623 2082 Size: 1529 1012 3010 Fragments arranged by size: 3010 1529 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5551 BstBI TT'CG_AA Cuts at: 4355 4355 Size: 5551 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5551 BstYI r'GATC_y Cuts at: 1972 2108 2219 2350 2404 2822 2881 3248 3633 Size: 136 111 131 54 418 59 367 385 Cuts at: 3633 3650 5102 5188 5200 5546 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 418 385 367 346 136 131 111 86 59 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4999 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4812 Fragments arranged by size: 4812 683 56 BtsI GCAGTG_nn' Cuts at: 3067 3861 3881 3067 Size: 794 20 4737 Fragments arranged by size: 4737 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5516 DraI TTT'AAA Cuts at: 2855 2855 Size: 5551 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4558 809 Size: 507 3242 1802 Fragments arranged by size: 3242 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2998 3887 5516 Size: 54 1425 50 275 18 1168 889 1629 Cuts at: 5516 8 Size: 43 Fragments arranged by size: 1629 1425 1168 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2998 1537 Size: 275 1186 4090 Fragments arranged by size: 4090 1186 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3481 1353 Size: 2128 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4138 4512 4658 387 Size: 1064 138 2549 374 146 1280 Fragments arranged by size: 2549 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3614 4211 4998 5266 1239 Size: 393 1982 597 787 268 1524 Fragments arranged by size: 1982 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2571 2860 3238 2571 Size: 289 378 4884 Fragments arranged by size: 4884 378 289 FspI TGC'GCA Cuts at: 4057 4057 Size: 5551 HaeII r_GCGC'y Cuts at: 1678 4698 1678 Size: 3020 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 4192 4224 4266 Size: 32 135 32 515 32 2073 32 42 Cuts at: 4266 4298 1373 Size: 32 2626 Fragments arranged by size: 2626 2073 515 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2895 3141 669 Size: 988 281 957 246 3079 Fragments arranged by size: 3079 988 957 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5551 HpaI GTT'AAC Cuts at: 3141 3141 Size: 5551 KpnI G_GTAC'C Cuts at: 2876 2876 Size: 5551 MfeI C'AATT_G Cuts at: 3150 3150 Size: 5551 MluI A'CGCG_T Cuts at: 5427 5427 Size: 5551 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2090 4665 4849 1305 Size: 785 2575 184 2007 Fragments arranged by size: 2575 2007 785 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3729 Fragments arranged by size: 3729 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2627 2649 3509 3868 4027 518 Size: 648 1461 22 860 359 159 2042 Fragments arranged by size: 2042 1461 860 648 359 159 22 MspA1I CmG'CkG Cuts at: 1606 2967 3644 4792 5037 1606 Size: 1361 677 1148 245 2120 Fragments arranged by size: 2120 1361 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4999 NdeI CA'TA_TG Cuts at: 387 387 Size: 5551 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5551 NotI GC'GGCC_GC Cuts at: 2998 2998 Size: 5551 NspI r_CATG'y Cuts at: 1132 1229 2909 4454 1132 Size: 97 1680 1545 2229 Fragments arranged by size: 2229 1680 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5551 PciI A'CATG_T Cuts at: 4450 4450 Size: 5551 PflMI CCAn_nnn'nTGG Cuts at: 1108 2441 1108 Size: 1333 4218 PmeI GTTT'AAAC Cuts at: 2855 2855 Size: 5551 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2065 2097 3620 3652 5133 5165 2065 Size: 32 1523 32 1481 32 2451 Fragments arranged by size: 2451 1523 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5551 PsiI TTA'TAA Cuts at: 3121 3121 Size: 5551 PstI C_TGCA'G Cuts at: 830 2903 830 Size: 2073 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5551 SalI G'TCGA_C Cuts at: 1936 2893 1936 Size: 957 4594 SbfI CC_TGCA'GG Cuts at: 2903 2903 Size: 5551 ScaI AGT'ACT Cuts at: 3799 5511 3799 Size: 1712 3839 SfcI C'TryA_G Cuts at: 826 1045 2833 2899 4034 4715 4906 826 Size: 219 1788 66 1135 681 191 1471 Fragments arranged by size: 1788 1471 1135 681 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5551 SmaI CCC'GGG Cuts at: 1872 2878 1872 Size: 1006 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2481 3655 4556 4818 820 Size: 197 926 538 1174 901 262 1553 Fragments arranged by size: 1553 1174 926 901 538 262 197 SnaBI TAC'GTA Cuts at: 493 3342 493 Size: 2849 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5551 SphI G_CATG'C Cuts at: 1132 2909 1132 Size: 1777 3774 SspI AAT'ATT Cuts at: 5 52 3475 5 Size: 47 3423 2081 Fragments arranged by size: 3423 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5551 StyI C'CwwG_G Cuts at: 513 1065 2365 2990 513 Size: 552 1300 625 3074 Fragments arranged by size: 3074 1300 625 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3578 3595 3748 3933 4378 325 Size: 1255 1998 17 153 185 445 1498 Fragments arranged by size: 1998 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2388 3797 5509 Size: 274 80 33 51 1443 411 1409 1712 Cuts at: 5509 96 Size: 138 Fragments arranged by size: 1712 1443 1409 411 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2129 2161 5511 5543 2129 Size: 32 3350 32 2137 Fragments arranged by size: 3350 2137 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5551 XbaI T'CTAG_A Cuts at: 2887 2887 Size: 5551 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5551 XhoI C'TCGA_G Cuts at: 1943 2481 1943 Size: 538 5013 XmaI C'CCGG_G Cuts at: 1870 2876 1870 Size: 1006 4545 XmnI GAAnn'nnTTC Cuts at: 2299 3680 2299 Size: 1381 4170 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2702 3359 276 Size: 53 83 186 936 1168 657 2468 Fragments arranged by size: 2468 1168 936 657 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI