################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DpnII DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI NarI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 91 CGCG CG^CG AcsI 152 GAATTC [GA]^AATT[CT] AluI 249 AGCT AG^CT AlwNI 139 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 152 GAATTC [GA]^AATT[CT] AspHI 312 GAGCAC G[AT]GC[AT]^C BmyI 312 GAGCAC G[AGT]GC[ACT]^C BsiHKAI 312 GAGCAC G[AT]GC[AT]^C BsmAI 254 GTCTC GTCTC(1/5) BsmFI 189 GTCCC GGGAC(10/14) Bsp1286I 312 GAGCAC G[AGT]GC[ACT]^C BsrFI 86 GCCGGC [GA]^CCGG[CT] BsrI 129 CCAGT ACTGG(1/-1) BssHII 90 GCGCGC G^CGCGC BstAPI 138 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 72 CCTGG CC^[AT]GG BstUI 91 CGCG CG^CG Cfr10I 86 GCCGGC [GA]^CCGG[CT] DraII 187 AGGTCCC [GA]G^G[ACGT]CC[CT] EaeI 77 TGGCCG [CT]^GGCC[GA] EcoO109I 187 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 152 GAATTC G^AATTC EcoRII 72 CCTGG ^CC[AT]GG EcoT14I 272 CCATGG C^C[AT][AT]GG HaeII 54 GGCGCT [GA]GCGC^[CT] HgaI 103 GCGTC GACGC(5/10) HindIII 248 AAGCTT A^AGCTT HphI 156 TCACC GGTGA(8/7) MaeIII 112 GTCAC ^GT[ACGT]AC MboII 243 GAAGA GAAGA(8/7) MnlI 159 CCTC CCTC(7/6) MvaI 72 CCTGG CC^[AT]GG MvnI 91 CGCG CG^CG NaeI 86 GCCGGC GCC^GGC NcoI 272 CCATGG C^CATGG NgoMIV 86 GCCGGC G^CCGGC NlaIII 273 CATG CATG^ PpuMI 187 AGGTCCC [GA]G^G[AT]CC[CT] PstI 145 CTGCAG CTGCA^G SfaNI 265 GCATC GCATC(5/9) SfcI 145 CTGCAG C^T[GA][CT]AG StyI 272 CCATGG C^C[AT][AT]GG TaqI 290 TCGA T^CGA Tsp45I 112 GTCAC ^GT[GC]AC Tsp509I 153 AATT ^AATT TthHB8I 290 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AvaII 188 GGTCC G^G[AT]CC 196 GGACC BcnI 38 CCCGG CC^[GC]GG 193 CCCGG BstF5I 168 CATCC GGATG(2/0) 266 CATCC DsaI 107 CCGTGG C^C[GA][CT]GG 272 CCATGG FauI 262 CCCGC CCCGC(4/6) 48 GCGGG FokI 168 CATCC GGATG(9/13) 266 CATCC HaeIII 3 GGCC GG^CC 78 GGCC NciI 38 CCCGG CC^[GC]GG 193 CCCGG SmlI 160 CTCAAG C^T[CT][GA]AG 232 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AciI BbvI BsaJI BsiYI BslI BssKI Cac8I CfoI Cfr13I Fnu4HI HapII HhaI HinP1I HpaII ItaI MspI MwoI NlaIV Sau96I ScrFI TseI TspRI