Restriction map for
pFN21AE0628
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssKI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRII EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaII KasI
KpnI KspI MaeII MaeIII MamI
MfeI MflI MluI MroI MspI
MunI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI ScrFI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StyI
SwaI TfiI Tsp45I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 463 GTAC GT^AC
AseI 280 ATTAAT AT^TAAT
AsnI 280 ATTAAT AT^TAAT
Asp700I 26 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 514 CTCGGG C^[CT]CG[GA]G
AvaII 956 GGTCC G^G[AT]CC
BfaI 115 CTAG C^TAG
BpmI 608 CTCCAG CTGGAG(16/14)
BsmAI 546 GTCTC GTCTC(1/5)
BsoBI 514 CTCGGG C^[CT]CG[GA]G
Bsp1407I 462 TGTACA T^GTACA
BspHI 288 TCATGA T^CATGA
BspMI 340 GCAGGT ACCTGC(4/8)
BsrGI 462 TGTACA T^GTACA
BssSI 235 CACGAG CACGAG(-5/-1)
Cfr13I 956 GGTCC G^G[ACGT]CC
DraI 474 TTTAAA TTT^AAA
DraII 955 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 209 GACAGGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 203 GAAGAG CTCTTC(1/4)
EcoO109I 955 AGGTCCT [GA]G^G[ACGT]CC[CT]
HincII 684 GTTAAC GT[CT]^[GA]AC
HindII 684 GTTAAC GT[CT]^[GA]AC
HpaI 684 GTTAAC GTT^AAC
Ksp632I 203 GAAGAG CTCTTC(1/4)
MaeI 115 CTAG C^TAG
MslI 562 CATGCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 790 CAGCTG C[AC]G^C[GT]G
NlaIV 994 GGAACC GG[ACGT]^[ACGT]CC
NspI 561 GCATGC [GA]CATG^[CT]
PpuMI 955 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 280 ATTAAT AT^TAAT
PstI 338 CTGCAG CTGCA^G
PvuII 790 CAGCTG CAG^CTG
RcaI 288 TCATGA T^CATGA
RsaI 463 GTAC GT^AC
Sau96I 956 GGTCC G^G[ACGT]CC
SmlI 743 CTCAAG C^T[CT][GA]AG
SphI 561 GCATGC GCATG^C
SspBI 462 TGTACA T^GTACA
StuI 36 AGGCCT AGG^CCT
TspRI 883 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 26 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwI 628 GATCC GGATC(4/5)
942 GGATC
BalI 165 TGGCCA TGG^CCA
794 TGGCCA
BbsI 964 GAAGAC GAAGAC(2/6)
1012 GAAGAC
BpuAI 964 GAAGAC GAAGAC(2/6)
1012 GAAGAC
BsiYI 510 CCCTCTCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
700 CCATTTGTGGG
BslI 510 CCCTCTCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
700 CCATTTGTGGG
BsrI 163 ACTGG ACTGG(1/-1)
923 CCAGT
Cac8I 561 GCATGC GC[ACGT]^[ACGT]GC
792 GCTGGC
DpnII 628 GATC ^GATC
943 GATC
EaeI 165 TGGCCA [CT]^GGCC[GA]
794 TGGCCA
HinfI 410 GACTC G^A[ACGT]TC
1072 GACTC
MboI 628 GATC ^GATC
943 GATC
MluNI 165 TGGCCA TGG^CCA
794 TGGCCA
MscI 165 TGGCCA TGG^CCA
794 TGGCCA
NdeII 628 GATC ^GATC
943 GATC
PleI 410 GACTC GAGTC(4/5)
1072 GACTC
Sau3AI 628 GATC ^GATC
943 GATC
SfcI 338 CTGCAG C^T[GA][CT]AG
877 CTACAG
SspI 599 AATATT AAT^ATT
658 AATATT
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
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AcsI AluI ApoI BbvI BseRI
BsmFI DdeI Fnu4HI HaeIII HphI
ItaI MboII MnlI MseI NlaIII
TaqI Tru9I TseI Tsp509I TthHB8I