(Circular) (Six-base) MAPSORT of: pFN21AB3477.seq Check: 9653 from: 1 to: 5308 pFN21AB3477 5308 bp With 182 enzymes: SgfI * October 20, 2009 10:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2274 3118 278 Size: 53 83 186 936 738 844 2468 Fragments arranged by size: 2468 936 844 738 186 83 53 AccI GT'mk_AC Cuts at: 1937 2651 1937 Size: 714 4594 Acc65I G'GTAC_C Cuts at: 2629 2629 Size: 5308 AclI AA'CG_TT Cuts at: 1563 3435 3808 1563 Size: 1872 373 3063 Fragments arranged by size: 3063 1872 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3371 4755 5023 1632 Size: 1739 1384 268 1917 Fragments arranged by size: 1917 1739 1384 268 AfeI AGC'GCT Cuts at: 2210 2210 Size: 5308 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5111 AflIII A'CryG_T Cuts at: 1203 4207 5184 1203 Size: 3004 977 1327 Fragments arranged by size: 3004 1327 977 AgeI A'CCGG_T Cuts at: 4139 4139 Size: 5308 AhdI GACnn_n'nnGTC Cuts at: 4037 4037 Size: 5308 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5308 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5308 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2468 2500 2468 Size: 32 5276 AlwNI CAG_nnn'CTG Cuts at: 3051 4623 3051 Size: 1572 3736 ApaLI G'TGCA_C Cuts at: 3365 4521 3365 Size: 1156 4152 ApoI r'AATT_y Cuts at: 1493 2414 2617 2843 2995 5104 1493 Size: 921 203 226 152 2109 1697 Fragments arranged by size: 2109 1697 921 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 3862 160 Size: 872 2830 1606 Fragments arranged by size: 2830 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5308 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2633 1870 Size: 73 67 623 4545 Fragments arranged by size: 4545 623 73 67 BamHI G'GATC_C Cuts at: 2638 3005 2638 Size: 367 4941 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2629 4084 618 Size: 325 217 627 842 1455 1842 Fragments arranged by size: 1842 1455 842 627 325 217 BanII G_rGCy'C Cuts at: 1391 2627 1391 Size: 1236 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3499 3533 1903 Size: 34 1562 34 3678 Fragments arranged by size: 3678 1562 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3200 4410 3200 Size: 1210 4098 BclI T'GATC_A Cuts at: 1548 1548 Size: 5308 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3919 136 Size: 107 122 71 631 605 2247 1525 Fragments arranged by size: 2247 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2184 5303 2184 Size: 3119 2189 BlpI GC'TnA_GC Cuts at: 2725 2725 Size: 5308 Bme1580I G_kGCm'C Cuts at: 1790 3369 4525 1790 Size: 1579 1156 2573 Fragments arranged by size: 2573 1579 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5308 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3039 3997 442 Size: 416 53 83 399 24 1622 958 1753 Fragments arranged by size: 1753 1622 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5308 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3968 1239 Size: 2729 2579 Bpu10I CC'TnA_GC Cuts at: 2379 2379 Size: 5308 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3433 4298 4596 3433 Size: 865 298 4145 Fragments arranged by size: 4145 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2441 3971 882 Size: 617 942 1530 2219 Fragments arranged by size: 2219 1530 942 617 BsaAI yAC'GTr Cuts at: 493 1204 2232 3099 493 Size: 711 1028 867 2702 Fragments arranged by size: 2702 1028 867 711 BsaBI GATnn'nnATC Cuts at: 2432 5302 2432 Size: 2870 2438 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2271 3115 3497 4132 Size: 53 83 186 936 738 844 382 635 Cuts at: 4132 275 Size: 1451 Fragments arranged by size: 1451 936 844 738 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2145 3741 4139 4413 4560 4971 2145 Size: 1596 398 274 147 411 2482 Fragments arranged by size: 2482 1596 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5083 Fragments arranged by size: 5083 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5308 BseYI C'CCAG_C Cuts at: 4511 5007 4511 Size: 496 4812 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4735 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2127 2758 3519 4547 1540 Size: 275 186 126 631 761 1028 2301 Fragments arranged by size: 2301 1028 761 631 275 186 126 BsiHKAI G_wGCw'C Cuts at: 3369 3454 4525 3369 Size: 85 1071 4152 Fragments arranged by size: 4152 1071 85 BsmI GAATG_Cn' Cuts at: 2819 2912 2819 Size: 93 5215 BsmBI CGTCTCn'nnnn_ Cuts at: 2390 2390 Size: 5308 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2627 3369 3454 4525 1391 Size: 399 837 742 85 1071 2174 Fragments arranged by size: 2174 1071 837 742 399 85 BspEI T'CCGG_A Cuts at: 2145 4971 2145 Size: 2826 2482 BspHI T'CATG_A Cuts at: 3197 4927 3197 Size: 1730 3578 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2663 844 Size: 1819 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2383 3195 1394 Size: 429 560 812 3507 Fragments arranged by size: 3507 812 560 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3803 3977 65 Size: 1183 2555 174 1396 Fragments arranged by size: 2555 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3952 4139 1700 Size: 110 2142 187 2869 Fragments arranged by size: 2869 2142 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5308 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5308 BssSI C'ACGA_G Cuts at: 3368 4380 3368 Size: 1012 4296 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5308 BstBI TT'CG_AA Cuts at: 4112 4112 Size: 5308 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5308 BstYI r'GATC_y Cuts at: 1972 2142 2184 2638 3005 3390 3407 4859 4945 Size: 170 42 454 367 385 17 1452 86 Cuts at: 4945 4957 5303 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 454 385 367 346 170 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2151 2248 513 Size: 552 939 147 97 3573 Fragments arranged by size: 3573 939 552 147 97 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4569 Fragments arranged by size: 4569 683 56 BtsI GCAGTG_nn' Cuts at: 2824 3618 3638 2824 Size: 794 20 4494 Fragments arranged by size: 4494 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5308 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5273 DraI TTT'AAA Cuts at: 2612 2612 Size: 5308 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4315 809 Size: 507 2999 1802 Fragments arranged by size: 2999 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2755 3644 5273 Size: 54 1425 50 275 18 925 889 1629 Cuts at: 5273 8 Size: 43 Fragments arranged by size: 1629 1425 925 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2755 1537 Size: 275 943 4090 Fragments arranged by size: 4090 943 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2033 2472 3238 1353 Size: 680 439 766 3423 Fragments arranged by size: 3423 766 680 439 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3895 4269 4415 387 Size: 1064 138 2306 374 146 1280 Fragments arranged by size: 2306 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3371 3968 4755 5023 1239 Size: 393 1739 597 787 268 1524 Fragments arranged by size: 1739 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2244 2244 Size: 5308 EcoRI G'AATT_C Cuts at: 2617 2995 2617 Size: 378 4930 FspI TGC'GCA Cuts at: 3814 3814 Size: 5308 HaeII r_GCGC'y Cuts at: 1678 2212 2457 4455 1678 Size: 534 245 1998 2531 Fragments arranged by size: 2531 1998 534 245 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3949 3981 4023 4055 1373 Size: 32 135 32 2377 32 42 32 2626 Fragments arranged by size: 2626 2377 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2296 2470 2652 2898 669 Size: 988 281 358 174 182 246 3079 Fragments arranged by size: 3079 988 358 281 246 182 174 HindIII A'AGCT_T Cuts at: 748 748 Size: 5308 HpaI GTT'AAC Cuts at: 2898 2898 Size: 5308 KpnI G_GTAC'C Cuts at: 2633 2633 Size: 5308 MfeI C'AATT_G Cuts at: 2907 2907 Size: 5308 MluI A'CGCG_T Cuts at: 5184 5184 Size: 5308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4422 4606 1305 Size: 3117 184 2007 Fragments arranged by size: 3117 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3486 Fragments arranged by size: 3486 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3266 3625 3784 518 Size: 648 2100 359 159 2042 Fragments arranged by size: 2100 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2055 2198 2724 3401 4549 4794 1606 Size: 449 143 526 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 526 449 245 143 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3817 Fragments arranged by size: 3817 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5308 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5308 NotI GC'GGCC_GC Cuts at: 2755 2755 Size: 5308 NspI r_CATG'y Cuts at: 1132 1229 2666 4211 1132 Size: 97 1437 1545 2229 Fragments arranged by size: 2229 1545 1437 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5308 PciI A'CATG_T Cuts at: 4207 4207 Size: 5308 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5308 PmeI GTTT'AAAC Cuts at: 2612 2612 Size: 5308 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3377 3409 4890 4922 3377 Size: 32 1481 32 3763 Fragments arranged by size: 3763 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2244 2244 Size: 5308 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5308 PsiI TTA'TAA Cuts at: 2878 2878 Size: 5308 PstI C_TGCA'G Cuts at: 830 2184 2660 830 Size: 1354 476 3478 Fragments arranged by size: 3478 1354 476 PvuI CG_AT'CG Cuts at: 2001 2127 2001 Size: 126 5182 RsrII CG'GwC_CG Cuts at: 2148 2148 Size: 5308 SalI G'TCGA_C Cuts at: 1936 2650 1936 Size: 714 4594 SanDI GG'GwC_CC Cuts at: 2244 2244 Size: 5308 SbfI CC_TGCA'GG Cuts at: 2660 2660 Size: 5308 ScaI AGT'ACT Cuts at: 3556 5268 3556 Size: 1712 3596 SfcI C'TryA_G Cuts at: 826 1045 2180 2656 3791 4472 4663 826 Size: 219 1135 476 1135 681 191 1471 Fragments arranged by size: 1471 1135 1135 681 476 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5308 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5308 SmaI CCC'GGG Cuts at: 1872 2012 2635 1872 Size: 140 623 4545 Fragments arranged by size: 4545 623 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 3412 4313 4575 820 Size: 197 926 1469 901 262 1553 Fragments arranged by size: 1553 1469 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3099 493 Size: 2606 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5308 SphI G_CATG'C Cuts at: 1132 2666 1132 Size: 1534 3774 SspI AAT'ATT Cuts at: 5 52 2352 3232 5 Size: 47 2300 880 2081 Fragments arranged by size: 2300 2081 880 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5308 StyI C'CwwG_G Cuts at: 513 1065 2004 2457 2747 513 Size: 552 939 453 290 3074 Fragments arranged by size: 3074 939 552 453 290 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3335 3352 3505 3690 4135 325 Size: 1255 1755 17 153 185 445 1498 Fragments arranged by size: 1755 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3554 5266 96 Size: 274 80 33 51 1443 1577 1712 138 Fragments arranged by size: 1712 1577 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5268 5300 5268 Size: 32 5276 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5308 XbaI T'CTAG_A Cuts at: 2644 2644 Size: 5308 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2128 2128 Size: 5308 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5308 XmaI C'CCGG_G Cuts at: 1870 2010 2633 1870 Size: 140 623 4545 Fragments arranged by size: 4545 623 140 XmnI GAAnn'nnTTC Cuts at: 3437 3437 Size: 5308 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2272 3116 276 Size: 53 83 186 936 738 844 2468 Fragments arranged by size: 2468 936 844 738 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI