################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BbeI BcgI BclI BfrI BlnI BlpI Bpu1102I BsiEI BsiWI BsmBI BspDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KasI KpnI MfeI MluI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 2416 GTCGAC GT^[AC][GT]AC AccIII 1170 TCCGGA T^CCGGA AcyI 862 GACGCC G[GA]^CG[CT]C Alw44I 1900 GTGCAC G^TGCAC Aor51HI 1814 AGCGCT AGC^GCT ApaLI 1900 GTGCAC G^TGCAC AviII 2042 TGCGCA TGC^GCA BbrPI 1688 CACGTG CAC^GTG BbvCI 517 GCTGAGG CCTCAGC(-5/-2) BfaI 778 CTAG C^TAG BsaBI 883 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 862 GACGCC G[GA]^CG[CT]C BsaWI 1170 TCCGGA [AT]^CCGG[AT] BseAI 1170 TCCGGA T^CCGGA BspEI 1170 TCCGGA T^CCGGA BspHI 1190 TCATGA T^CATGA BspLU11I 1868 ACATGT A^CATGT BsrBI 3112 GAGCGG CCGCTC(-3/-3) BsrFI 404 GCCGGC [GA]^CCGG[CT] BssSI 2701 CTCGTG CACGAG(-5/-1) Cfr10I 404 GCCGGC [GA]^CCGG[CT] DraIII 2743 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1814 AGCGCT AGC^GCT FspI 2042 TGCGCA TGC^GCA Hin1I 862 GACGCC G[GA]^CG[CT]C HincII 2416 GTCGAC GT[CT]^[GA]AC HindII 2416 GTCGAC GT[CT]^[GA]AC KspI 1650 CCGCGG CCGC^GG MaeI 778 CTAG C^TAG MamI 883 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1170 TCCGGA T^CCGGA NaeI 404 GCCGGC GCC^GGC NgoMIV 404 GCCGGC G^CCGGC PmaCI 1688 CACGTG CAC^GTG PmlI 1688 CACGTG CAC^GTG RcaI 1190 TCATGA T^CATGA SacII 1650 CCGCGG CCGC^GG SalI 2416 GTCGAC G^TCGAC SexAI 561 ACCTGGT A^CC[AT]GGT SfiI 407 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 3536 GCATGC GCATG^C SspI 2429 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 1651 CGCG CG^CG 1888 CGCG AspI 976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2947 GACCCTGTC BamHI 255 GGATCC G^GATCC 2862 GGATCC BbsI 249 GAAGAC GAAGAC(2/6) 772 GAAGAC BciVI 453 GTATCC GTATCC(6/5) 805 GTATCC BpuAI 249 GAAGAC GAAGAC(2/6) 772 GAAGAC BsmI 3089 GAATGC GAATGC(1/-1) 3596 GCATTC BsrDI 1035 CATTGC GCAATG(2/0) 3143 CATTGC BstUI 1651 CGCG CG^CG 1888 CGCG EcoNI 1962 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3390 CCTCAAGCAGG HaeII 1814 AGCGCT [GA]GCGC^[CT] 3602 AGCGCC MvnI 1651 CGCG CG^CG 1888 CGCG NcoI 132 CCATGG C^CATGG 1997 CCATGG PflFI 976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2947 GACCCTGTC SapI 1788 GAAGAGC GCTCTTC(1/4) 1947 GCTCTTC SmaI 1724 CCCGGG CCC^GGG 3530 CCCGGG Tth111I 976 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 2947 GACCCTGTC XmaCI 1724 CCCGGG C^CCGGG 3530 CCCGGG XmaI 1724 CCCGGG C^CCGGG 3530 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BglI BglII BmyI BpmI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII