(Circular) (Six-base) MAPSORT of: pFN21AE4596.seq Check: 1503 from: 1 to: 8443 pFN21AE4596 8443 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6253 278 Size: 53 83 186 936 4717 2468 Fragments arranged by size: 4717 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4422 5786 1937 Size: 2485 1364 4594 Fragments arranged by size: 4594 2485 1364 Acc65I G'GTAC_C Cuts at: 5764 5764 Size: 8443 AclI AA'CG_TT Cuts at: 1563 6570 6943 1563 Size: 5007 373 3063 Fragments arranged by size: 5007 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3434 3940 4532 4752 4819 5099 6506 7890 Size: 1802 506 592 220 67 280 1407 1384 Cuts at: 7890 8158 1632 Size: 268 1917 Fragments arranged by size: 1917 1802 1407 1384 592 506 280 268 220 67 AfeI AGC'GCT Cuts at: 3821 3821 Size: 8443 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8246 AflIII A'CryG_T Cuts at: 1203 3692 3873 5413 7342 8319 1203 Size: 2489 181 1540 1929 977 1327 Fragments arranged by size: 2489 1929 1540 1327 977 181 AgeI A'CCGG_T Cuts at: 7274 7274 Size: 8443 AhdI GACnn_n'nnGTC Cuts at: 7172 7172 Size: 8443 AleI CACnn'nnGTG Cuts at: 1166 4796 1166 Size: 3630 4813 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8443 AlwNI CAG_nnn'CTG Cuts at: 2344 2621 3868 4360 4679 4906 5077 5611 6186 Size: 277 1247 492 319 227 171 534 575 Cuts at: 6186 7758 2344 Size: 1572 3029 Fragments arranged by size: 3029 1572 1247 575 534 492 319 277 227 171 ApaI G_GGCC'C Cuts at: 3644 5011 5536 3644 Size: 1367 525 6551 Fragments arranged by size: 6551 1367 525 ApaLI G'TGCA_C Cuts at: 3905 6500 7656 3905 Size: 2595 1156 4692 Fragments arranged by size: 4692 2595 1156 ApoI r'AATT_y Cuts at: 1493 4856 5483 5500 5752 5978 6130 8239 1493 Size: 3363 627 17 252 226 152 2109 1697 Fragments arranged by size: 3363 2109 1697 627 252 226 152 17 AseI AT'TA_AT Cuts at: 160 1032 6997 160 Size: 872 5965 1606 Fragments arranged by size: 5965 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8443 AvaI C'yCGr_G Cuts at: 1870 1943 2760 3531 3729 4175 5535 5768 1870 Size: 73 817 771 198 446 1360 233 4545 Fragments arranged by size: 4545 1360 817 771 446 233 198 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2793 2826 2793 Size: 33 8410 BamHI G'GATC_C Cuts at: 2260 4867 5773 6140 2260 Size: 2607 906 367 4563 Fragments arranged by size: 4563 2607 906 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4142 4687 5672 5764 7219 Size: 325 217 627 2355 545 985 92 1455 Cuts at: 7219 618 Size: 1842 Fragments arranged by size: 2355 1842 1455 985 627 545 325 217 92 BanII G_rGCy'C Cuts at: 1391 2076 2119 2293 3513 3644 3885 5004 5011 Size: 685 43 174 1220 131 241 1119 7 Cuts at: 5011 5041 5286 5363 5536 5568 5762 1391 Size: 30 245 77 173 32 194 4072 Fragments arranged by size: 4072 1220 1119 685 245 241 194 174 173 131 77 43 32 30 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2261 2784 928 Size: 971 362 523 6587 Fragments arranged by size: 6587 971 523 362 BbvCI CC'TCA_GC Cuts at: 2523 2523 Size: 8443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6634 6668 1903 Size: 34 4697 34 3678 Fragments arranged by size: 4697 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2469 2821 6335 7545 2469 Size: 352 3514 1210 3367 Fragments arranged by size: 3514 3367 1210 352 BclI T'GATC_A Cuts at: 1548 1548 Size: 8443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2419 3840 4735 Size: 107 122 71 631 605 747 1421 895 Cuts at: 4735 7054 136 Size: 2319 1525 Fragments arranged by size: 2319 1525 1421 895 747 631 605 122 107 71 BglII A'GATC_T Cuts at: 2161 2500 2548 3426 4821 8438 2161 Size: 339 48 878 1395 3617 2166 Fragments arranged by size: 3617 2166 1395 878 339 48 BlpI GC'TnA_GC Cuts at: 5860 5860 Size: 8443 Bme1580I G_kGCm'C Cuts at: 1790 2091 2158 3462 3644 3700 3909 4176 4692 Size: 301 67 1304 182 56 209 267 516 Cuts at: 4692 5011 5222 5536 6504 7660 1790 Size: 319 211 314 968 1156 2573 Fragments arranged by size: 2573 1304 1156 968 516 319 314 301 267 211 209 182 67 56 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8443 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2101 2288 2354 Size: 416 53 83 399 24 684 187 66 Cuts at: 2354 2425 3694 4225 5065 6174 7132 442 Size: 71 1269 531 840 1109 958 1753 Fragments arranged by size: 1753 1269 1109 958 840 684 531 416 399 187 83 71 66 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8443 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3769 4342 3769 Size: 573 7870 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2022 2103 2133 2387 2400 2598 3275 3485 Size: 783 81 30 254 13 198 677 210 Cuts at: 3485 4748 5039 7103 1239 Size: 1263 291 2064 2579 Fragments arranged by size: 2579 2064 1263 783 677 291 254 210 198 81 30 13 Bpu10I CC'TnA_GC Cuts at: 2523 2636 3098 4920 2523 Size: 113 462 1822 6046 Fragments arranged by size: 6046 1822 462 113 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3407 3917 5381 6568 7433 7731 3407 Size: 510 1464 1187 865 298 4119 Fragments arranged by size: 4119 1464 1187 865 510 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2274 3986 4457 4594 7106 882 Size: 617 775 1712 471 137 2512 2219 Fragments arranged by size: 2512 2219 1712 775 617 471 137 BsaAI yAC'GTr Cuts at: 493 1204 3695 4245 5206 6234 493 Size: 711 2491 550 961 1028 2702 Fragments arranged by size: 2702 2491 1028 961 711 550 BsaBI GATnn'nnATC Cuts at: 2892 8437 2892 Size: 5545 2898 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2868 6250 6632 7267 Size: 53 83 186 936 1335 3382 382 635 Cuts at: 7267 275 Size: 1451 Fragments arranged by size: 3382 1451 1335 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3175 6876 7274 7548 7695 8106 3175 Size: 3701 398 274 147 411 3512 Fragments arranged by size: 3701 3512 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2280 2310 2602 2632 2858 Size: 30 165 30 877 30 292 30 226 Cuts at: 2858 2888 4046 4076 5649 5679 1178 Size: 30 1158 30 1573 30 3942 Fragments arranged by size: 3942 1573 1158 877 292 226 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2531 2540 2543 3332 3549 4321 4409 4582 Size: 1345 9 3 789 217 772 88 173 Cuts at: 4582 5036 5264 5336 1186 Size: 454 228 72 4293 Fragments arranged by size: 4293 1345 789 772 454 228 217 173 88 72 9 3 BseYI C'CCAG_C Cuts at: 2200 2284 2907 4218 4381 4443 4467 4930 5372 Size: 84 623 1311 163 62 24 463 442 Cuts at: 5372 5668 7646 8142 2200 Size: 296 1978 496 2501 Fragments arranged by size: 2501 1978 1311 623 496 463 442 296 163 84 62 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2948 3630 3854 4118 4175 1163 Size: 573 1212 682 224 264 57 5431 Fragments arranged by size: 5431 1212 682 573 264 224 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5893 6654 7682 1540 Size: 275 186 3892 761 1028 2301 Fragments arranged by size: 3892 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 2119 2232 2752 3471 3513 3885 3909 4803 Size: 43 113 520 719 42 372 24 894 Cuts at: 4803 5190 5286 5309 6504 6589 7660 2076 Size: 387 96 23 1195 85 1071 2859 Fragments arranged by size: 2859 1195 1071 894 719 520 387 372 113 96 85 43 42 24 23 BsmI GAATG_Cn' Cuts at: 5100 5601 5954 6047 5100 Size: 501 353 93 7496 Fragments arranged by size: 7496 501 353 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2091 2119 2158 2232 2293 2752 Size: 399 286 15 28 39 74 61 459 Cuts at: 2752 3462 3471 3513 3644 3700 3885 3909 4176 Size: 710 9 42 131 56 185 24 267 Cuts at: 4176 4692 4803 5004 5011 5041 5190 5222 5286 Size: 516 111 201 7 30 149 32 64 Cuts at: 5286 5309 5363 5536 5568 5762 6504 6589 7660 Size: 23 54 173 32 194 742 85 1071 Cuts at: 7660 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 710 516 459 399 286 267 201 194 185 173 149 131 111 85 74 64 61 56 54 42 39 32 32 30 28 24 23 15 9 7 BspEI T'CCGG_A Cuts at: 3175 8106 3175 Size: 4931 3512 BspHI T'CATG_A Cuts at: 3195 6332 8062 3195 Size: 3137 1730 3576 Fragments arranged by size: 3576 3137 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4086 4197 4434 4974 5798 844 Size: 3242 111 237 540 824 3489 Fragments arranged by size: 3489 3242 824 540 237 111 BsrBI CCG'CTC Cuts at: 1394 1823 5119 6330 1394 Size: 429 3296 1211 3507 Fragments arranged by size: 3507 3296 1211 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3039 5147 6938 7112 65 Size: 1183 1791 2108 1791 174 1396 Fragments arranged by size: 2108 1791 1791 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2409 7087 7274 1700 Size: 110 599 4678 187 2869 Fragments arranged by size: 4678 2869 599 187 110 BsrGI T'GTAC_A Cuts at: 96 3163 3225 5047 96 Size: 3067 62 1822 3492 Fragments arranged by size: 3492 3067 1822 62 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8443 BssSI C'ACGA_G Cuts at: 4706 6503 7515 4706 Size: 1797 1012 5634 Fragments arranged by size: 5634 1797 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8443 BstBI TT'CG_AA Cuts at: 7247 7247 Size: 8443 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8443 BstXI CCAn_nnnn'nTGG Cuts at: 2863 3046 3611 4381 5363 2863 Size: 183 565 770 982 5943 Fragments arranged by size: 5943 982 770 565 183 BstYI r'GATC_y Cuts at: 1972 2041 2161 2260 2500 2548 3207 3426 3801 Size: 69 120 99 240 48 659 219 375 Cuts at: 3801 4821 4867 5773 6140 6525 6542 7994 8080 Size: 1020 46 906 367 385 17 1452 86 Cuts at: 8080 8092 8438 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1020 906 659 385 375 367 346 240 219 120 99 86 69 48 46 17 12 Bsu36I CC'TnA_GG Cuts at: 3754 4283 5319 5580 3754 Size: 529 1036 261 6617 Fragments arranged by size: 6617 1036 529 261 BtgI C'CryG_G Cuts at: 513 1065 2067 2137 3655 3708 3838 3961 4002 Size: 552 1002 70 1518 53 130 123 41 Cuts at: 4002 4752 513 Size: 750 4204 Fragments arranged by size: 4204 1518 1002 750 552 130 123 70 53 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7704 Fragments arranged by size: 7704 683 56 BtsI GCAGTG_nn' Cuts at: 2700 2752 2855 4677 4890 5630 5959 6753 6773 Size: 52 103 1822 213 740 329 794 20 Cuts at: 6773 2700 Size: 4370 Fragments arranged by size: 4370 1822 794 740 329 213 103 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8443 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8408 DraI TTT'AAA Cuts at: 5747 5747 Size: 8443 DraIII CAC_nnn'GTG Cuts at: 4753 4753 Size: 8443 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7450 809 Size: 507 6134 1802 Fragments arranged by size: 6134 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2468 4411 5427 Size: 54 1425 50 275 18 638 1943 1016 Cuts at: 5427 5724 5890 6779 8408 8 Size: 297 166 889 1629 43 Fragments arranged by size: 1943 1629 1425 1016 889 638 297 275 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5890 1537 Size: 275 4078 4090 Fragments arranged by size: 4090 4078 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2409 3788 3815 3959 4298 6373 1353 Size: 1056 1379 27 144 339 2075 3423 Fragments arranged by size: 3423 2075 1379 1056 339 144 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4135 4226 7030 7404 7550 387 Size: 1064 138 2546 91 2804 374 146 1280 Fragments arranged by size: 2804 2546 1280 1064 374 146 138 91 EcoICRI GAG'CTC Cuts at: 2074 2117 3511 3883 5284 2074 Size: 43 1394 372 1401 5233 Fragments arranged by size: 5233 1401 1394 372 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2022 2103 2133 2387 2400 2598 3275 Size: 393 390 81 30 254 13 198 677 Cuts at: 3275 3434 3485 3940 4532 4748 4752 4819 5039 Size: 159 51 455 592 216 4 67 220 Cuts at: 5039 5099 6506 7103 7890 8158 1239 Size: 60 1407 597 787 268 1524 Fragments arranged by size: 1524 1407 787 677 597 592 455 393 390 268 254 220 216 198 159 81 67 60 51 30 13 4 EcoNI CCTnn'n_nnAGG Cuts at: 3971 5399 3971 Size: 1428 7015 EcoO109I rG'GnC_Cy Cuts at: 2711 2824 3094 3250 3640 3641 3865 4075 4126 Size: 113 270 156 390 1 224 210 51 Cuts at: 4126 4357 4654 4736 5574 5584 2711 Size: 231 297 82 838 10 5570 Fragments arranged by size: 5570 838 390 297 270 231 224 210 156 113 82 51 10 1 EcoRI G'AATT_C Cuts at: 5752 6130 5752 Size: 378 8065 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2570 4327 2570 Size: 1757 6686 FspI TGC'GCA Cuts at: 4049 6949 4049 Size: 2900 5543 HaeII r_GCGC'y Cuts at: 1678 3823 5611 7590 1678 Size: 2145 1788 1979 2531 Fragments arranged by size: 2531 2145 1979 1788 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2252 2280 2284 2312 2822 Size: 32 135 32 680 28 4 28 510 Cuts at: 2822 2854 2858 2890 7084 7116 7158 7190 1373 Size: 32 4 32 4194 32 42 32 2626 Fragments arranged by size: 4194 2626 680 510 135 42 32 32 32 32 32 32 28 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 4423 5787 6033 669 Size: 988 281 2485 1364 246 3079 Fragments arranged by size: 3079 2485 1364 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8443 HpaI GTT'AAC Cuts at: 6033 6033 Size: 8443 KpnI G_GTAC'C Cuts at: 5768 5768 Size: 8443 MfeI C'AATT_G Cuts at: 6042 6042 Size: 8443 MluI A'CGCG_T Cuts at: 8319 8319 Size: 8443 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2328 2474 2628 2633 5118 7557 7741 1305 Size: 1023 146 154 5 2485 2439 184 2007 Fragments arranged by size: 2485 2439 2007 1023 184 154 146 5 MscI TGG'CCA Cuts at: 10 64 1489 1832 2470 4413 5429 10 Size: 54 1425 343 638 1943 1016 3024 Fragments arranged by size: 3024 1943 1425 1016 638 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2861 4202 4796 6401 6760 6919 518 Size: 648 1695 1341 594 1605 359 159 2042 Fragments arranged by size: 2042 1695 1605 1341 648 594 359 159 MspA1I CmG'CkG Cuts at: 1606 2807 3169 3566 3657 3738 3856 4222 4443 Size: 1201 362 397 91 81 118 366 221 Cuts at: 4443 5598 5690 5859 6536 7684 7929 1606 Size: 1155 92 169 677 1148 245 2120 Fragments arranged by size: 2120 1201 1155 1148 677 397 366 362 245 221 169 118 92 91 81 NaeI GCC'GGC Cuts at: 2411 2411 Size: 8443 NcoI C'CATG_G Cuts at: 513 1065 2137 4002 513 Size: 552 1072 1865 4954 Fragments arranged by size: 4954 1865 1072 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8443 NgoMIV G'CCGG_C Cuts at: 2409 2409 Size: 8443 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8443 NotI GC'GGCC_GC Cuts at: 5890 5890 Size: 8443 NspI r_CATG'y Cuts at: 1132 1229 3877 4018 5545 5801 7346 1132 Size: 97 2648 141 1527 256 1545 2229 Fragments arranged by size: 2648 2229 1545 1527 256 141 97 PasI CC'CwG_GG Cuts at: 1348 3502 4085 5197 5363 1348 Size: 2154 583 1112 166 4428 Fragments arranged by size: 4428 2154 1112 583 166 PciI A'CATG_T Cuts at: 3873 7342 3873 Size: 3469 4974 PflMI CCAn_nnn'nTGG Cuts at: 1108 2344 2621 4753 5476 1108 Size: 1236 277 2132 723 4075 Fragments arranged by size: 4075 2132 1236 723 277 PfoI T'CCnGG_A Cuts at: 2620 3114 4071 4899 2620 Size: 494 957 828 6164 Fragments arranged by size: 6164 957 828 494 PmeI GTTT'AAAC Cuts at: 5747 5747 Size: 8443 PmlI CAC'GTG Cuts at: 3695 3695 Size: 8443 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6512 6544 8025 8057 6512 Size: 32 1481 32 6898 Fragments arranged by size: 6898 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2711 3250 3865 4075 4654 5574 2711 Size: 539 615 210 579 920 5580 Fragments arranged by size: 5580 920 615 579 539 210 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8443 PsiI TTA'TAA Cuts at: 6013 6013 Size: 8443 PspOMI G'GGCC_C Cuts at: 3640 5007 5532 3640 Size: 1367 525 6551 Fragments arranged by size: 6551 1367 525 PstI C_TGCA'G Cuts at: 830 2169 2747 3013 3819 4227 4684 4971 5795 Size: 1339 578 266 806 408 457 287 824 Cuts at: 5795 830 Size: 3478 Fragments arranged by size: 3478 1339 824 806 578 457 408 287 266 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8443 PvuII CAG'CTG Cuts at: 2807 3169 3566 3856 4222 4443 2807 Size: 362 397 290 366 221 6807 Fragments arranged by size: 6807 397 366 362 290 221 SacI G_AGCT'C Cuts at: 2076 2119 3513 3885 5286 2076 Size: 43 1394 372 1401 5233 Fragments arranged by size: 5233 1401 1394 372 43 SacII CC_GC'GG Cuts at: 3658 3658 Size: 8443 SalI G'TCGA_C Cuts at: 1936 4421 5785 1936 Size: 2485 1364 4594 Fragments arranged by size: 4594 2485 1364 SanDI GG'GwC_CC Cuts at: 5574 5574 Size: 8443 SapI GCTCTTCn'nnn_ Cuts at: 3788 3959 3788 Size: 171 8272 SbfI CC_TGCA'GG Cuts at: 5795 5795 Size: 8443 ScaI AGT'ACT Cuts at: 6691 8403 6691 Size: 1712 6731 SexAI A'CCwGG_T Cuts at: 2566 2566 Size: 8443 SfcI C'TryA_G Cuts at: 826 1045 2165 2309 2743 3009 3815 4223 4583 Size: 219 1120 144 434 266 806 408 360 Cuts at: 4583 4680 4967 5791 6926 7607 7798 826 Size: 97 287 824 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 824 806 681 434 408 360 287 266 219 191 144 97 SfiI GGCCn_nnn'nGGCC Cuts at: 2419 2419 Size: 8443 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8443 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8443 SmaI CCC'GGG Cuts at: 1872 3731 5537 5770 1872 Size: 1859 1806 233 4545 Fragments arranged by size: 4545 1859 1806 233 SmlI C'TyrA_G Cuts at: 820 1017 1943 3386 3932 5396 6547 7448 7710 Size: 197 926 1443 546 1464 1151 901 262 Cuts at: 7710 820 Size: 1553 Fragments arranged by size: 1553 1464 1443 1151 926 901 546 262 197 SnaBI TAC'GTA Cuts at: 493 6234 493 Size: 5741 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8443 SphI G_CATG'C Cuts at: 1132 5545 5801 1132 Size: 4413 256 3774 Fragments arranged by size: 4413 3774 256 SspI AAT'ATT Cuts at: 5 52 4436 6367 5 Size: 47 4384 1931 2081 Fragments arranged by size: 4384 2081 1931 47 StuI AGG'CCT Cuts at: 1517 3182 3752 3759 1517 Size: 1665 570 7 6201 Fragments arranged by size: 6201 1665 570 7 StyI C'CwwG_G Cuts at: 513 1065 2137 4002 4794 5882 513 Size: 552 1072 1865 792 1088 3074 Fragments arranged by size: 3074 1865 1088 1072 792 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2340 2654 3890 4944 5660 5753 6470 Size: 1255 760 314 1236 1054 716 93 717 Cuts at: 6470 6487 6640 6825 7270 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1236 1054 760 717 716 445 314 185 153 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3163 3225 5047 Size: 274 80 33 51 1443 1186 62 1822 Cuts at: 5047 6689 8401 96 Size: 1642 1712 138 Fragments arranged by size: 1822 1712 1642 1443 1186 274 138 80 62 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3242 3274 4046 4078 4521 4553 5649 5681 8403 Size: 32 772 32 443 32 1096 32 2722 Cuts at: 8403 8435 3242 Size: 32 3250 Fragments arranged by size: 3250 2722 1096 772 443 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2984 4955 1316 Size: 1668 1971 4804 Fragments arranged by size: 4804 1971 1668 XbaI T'CTAG_A Cuts at: 5779 5779 Size: 8443 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2919 2965 5456 2919 Size: 46 2491 5906 Fragments arranged by size: 5906 2491 46 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8443 XmaI C'CCGG_G Cuts at: 1870 3729 5535 5768 1870 Size: 1859 1806 233 4545 Fragments arranged by size: 4545 1859 1806 233 XmnI GAAnn'nnTTC Cuts at: 6572 6572 Size: 8443 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6251 276 Size: 53 83 186 936 4717 2468 Fragments arranged by size: 4717 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SfoI SrfI SwaI