(Linear) (Six-base) MAPSORT of: KIEE4596.seq Check: 5269 from: 1 to: 3738 KIEE4596.orf With 182 enzymes: SgfI * July 3, 2012 19:01 .. AccI GT'mk_AC Cuts at: 0 2417 3738 Size: 2417 1321 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1429 1935 2527 2747 2814 3094 3738 Size: 1429 506 592 220 67 280 644 Fragments arranged by size: 1429 644 592 506 280 220 67 AfeI AGC'GCT Cuts at: 0 1816 3738 Size: 1816 1922 AflIII A'CryG_T Cuts at: 0 1687 1868 3408 3738 Size: 1687 181 1540 330 Fragments arranged by size: 1687 1540 330 181 AleI CACnn'nnGTG Cuts at: 0 2791 3738 Size: 2791 947 AlwNI CAG_nnn'CTG Cuts at: 0 339 616 1863 2355 2674 2901 3072 3606 Size: 339 277 1247 492 319 227 171 534 Cuts at: 3606 3738 Size: 132 Fragments arranged by size: 1247 534 492 339 319 277 227 171 132 ApaI G_GGCC'C Cuts at: 0 1639 3006 3531 3738 Size: 1639 1367 525 207 Fragments arranged by size: 1639 1367 525 207 ApaLI G'TGCA_C Cuts at: 0 1900 3738 Size: 1900 1838 ApoI r'AATT_y Cuts at: 0 2851 3478 3495 3738 Size: 2851 627 17 243 Fragments arranged by size: 2851 627 243 17 AvaI C'yCGr_G Cuts at: 0 755 1526 1724 2170 3530 3738 Size: 755 771 198 446 1360 208 Fragments arranged by size: 1360 771 755 446 208 198 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 788 821 3738 Size: 788 33 2917 Fragments arranged by size: 2917 788 33 BamHI G'GATC_C Cuts at: 0 255 2862 3738 Size: 255 2607 876 Fragments arranged by size: 2607 876 255 BanI G'GyrC_C Cuts at: 0 2137 2682 3667 3738 Size: 2137 545 985 71 Fragments arranged by size: 2137 985 545 71 BanII G_rGCy'C Cuts at: 0 71 114 288 1508 1639 1880 2999 3006 Size: 71 43 174 1220 131 241 1119 7 Cuts at: 3006 3036 3281 3358 3531 3563 3738 Size: 30 245 77 173 32 175 Fragments arranged by size: 1220 1119 245 241 175 174 173 131 77 71 43 32 30 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 256 779 3738 Size: 256 523 2959 Fragments arranged by size: 2959 523 256 BbvCI CC'TCA_GC Cuts at: 0 518 3738 Size: 518 3220 BciVI GTATCCnnnnn_n' Cuts at: 0 464 816 3738 Size: 464 352 2922 Fragments arranged by size: 2922 464 352 BglI GCCn_nnn'nGGC Cuts at: 0 414 1835 2730 3738 Size: 414 1421 895 1008 Fragments arranged by size: 1421 1008 895 414 BglII A'GATC_T Cuts at: 0 156 495 543 1421 2816 3738 Size: 156 339 48 878 1395 922 Fragments arranged by size: 1395 922 878 339 156 48 Bme1580I G_kGCm'C Cuts at: 0 86 153 1457 1639 1695 1904 2171 2687 Size: 86 67 1304 182 56 209 267 516 Cuts at: 2687 3006 3217 3531 3738 Size: 319 211 314 207 Fragments arranged by size: 1304 516 319 314 267 211 209 207 182 86 67 56 BmrI ACTGGGnnnn_n' Cuts at: 0 96 283 349 420 1689 2220 3060 3738 Size: 96 187 66 71 1269 531 840 678 Fragments arranged by size: 1269 840 678 531 187 96 71 66 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1764 2337 3738 Size: 1764 573 1401 Fragments arranged by size: 1764 1401 573 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 17 98 128 382 395 593 1270 1480 Size: 17 81 30 254 13 198 677 210 Cuts at: 1480 2743 3034 3738 Size: 1263 291 704 Fragments arranged by size: 1263 704 677 291 254 210 198 81 30 17 13 Bpu10I CC'TnA_GC Cuts at: 0 518 631 1093 2915 3738 Size: 518 113 462 1822 823 Fragments arranged by size: 1822 823 518 462 113 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1402 1912 3376 3738 Size: 1402 510 1464 362 Fragments arranged by size: 1464 1402 510 362 BsaI GGTCTCn'nnnn_ Cuts at: 0 269 1981 2452 2589 3738 Size: 269 1712 471 137 1149 Fragments arranged by size: 1712 1149 471 269 137 BsaAI yAC'GTr Cuts at: 0 1690 2240 3201 3738 Size: 1690 550 961 537 Fragments arranged by size: 1690 961 550 537 BsaBI GATnn'nnATC Cuts at: 0 887 3738 Size: 887 2851 BsaHI Gr'CG_yC Cuts at: 0 863 3738 Size: 863 2875 BsaWI w'CCGG_w Cuts at: 0 1170 3738 Size: 1170 2568 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 275 305 597 627 853 883 2041 2071 Size: 275 30 292 30 226 30 1158 30 Cuts at: 2071 3644 3674 3738 Size: 1573 30 64 Fragments arranged by size: 1573 1158 292 275 226 64 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 526 535 538 1327 1544 2316 2404 2577 Size: 526 9 3 789 217 772 88 173 Cuts at: 2577 3031 3259 3331 3738 Size: 454 228 72 407 Fragments arranged by size: 789 772 526 454 407 228 217 173 88 72 9 3 BseYI C'CCAG_C Cuts at: 0 195 279 902 2213 2376 2438 2462 2925 Size: 195 84 623 1311 163 62 24 463 Cuts at: 2925 3367 3663 3738 Size: 442 296 75 Fragments arranged by size: 1311 623 463 442 296 195 163 84 75 62 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 943 1625 1849 2113 2170 3738 Size: 943 682 224 264 57 1568 Fragments arranged by size: 1568 943 682 264 224 57 BsiHKAI G_wGCw'C Cuts at: 0 71 114 227 747 1466 1508 1880 1904 Size: 71 43 113 520 719 42 372 24 Cuts at: 1904 2798 3185 3281 3304 3738 Size: 894 387 96 23 434 Fragments arranged by size: 894 719 520 434 387 372 113 96 71 43 42 24 23 BsmI GAATG_Cn' Cuts at: 0 3095 3596 3738 Size: 3095 501 142 Fragments arranged by size: 3095 501 142 Bsp1286I G_dGCh'C Cuts at: 0 71 86 114 153 227 288 747 1457 Size: 71 15 28 39 74 61 459 710 Cuts at: 1457 1466 1508 1639 1695 1880 1904 2171 2687 Size: 9 42 131 56 185 24 267 516 Cuts at: 2687 2798 2999 3006 3036 3185 3217 3281 3304 Size: 111 201 7 30 149 32 64 23 Cuts at: 3304 3358 3531 3563 3738 Size: 54 173 32 175 Fragments arranged by size: 710 516 459 267 201 185 175 173 149 131 111 74 71 64 61 56 54 42 39 32 32 30 28 24 23 15 9 7 BspEI T'CCGG_A Cuts at: 0 1170 3738 Size: 1170 2568 BspHI T'CATG_A Cuts at: 0 1190 3738 Size: 1190 2548 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2081 2192 2429 2969 3738 Size: 2081 111 237 540 769 Fragments arranged by size: 2081 769 540 237 111 BsrBI CCG'CTC Cuts at: 0 3114 3738 Size: 3114 624 BsrDI GCAATG_nn' Cuts at: 0 1034 3142 3738 Size: 1034 2108 596 Fragments arranged by size: 2108 1034 596 BsrFI r'CCGG_y Cuts at: 0 404 3738 Size: 404 3334 BsrGI T'GTAC_A Cuts at: 0 1158 1220 3042 3738 Size: 1158 62 1822 696 Fragments arranged by size: 1822 1158 696 62 BssSI C'ACGA_G Cuts at: 0 2701 3738 Size: 2701 1037 BstXI CCAn_nnnn'nTGG Cuts at: 0 858 1041 1606 2376 3358 3738 Size: 858 183 565 770 982 380 Fragments arranged by size: 982 858 770 565 380 183 BstYI r'GATC_y Cuts at: 0 36 156 255 495 543 1202 1421 1796 Size: 36 120 99 240 48 659 219 375 Cuts at: 1796 2816 2862 3738 Size: 1020 46 876 Fragments arranged by size: 1020 876 659 375 240 219 120 99 48 46 36 Bsu36I CC'TnA_GG Cuts at: 0 1749 2278 3314 3575 3738 Size: 1749 529 1036 261 163 Fragments arranged by size: 1749 1036 529 261 163 BtgI C'CryG_G Cuts at: 0 62 132 1650 1703 1833 1956 1997 2747 Size: 62 70 1518 53 130 123 41 750 Cuts at: 2747 3738 Size: 991 Fragments arranged by size: 1518 991 750 130 123 70 62 53 41 BtsI GCAGTG_nn' Cuts at: 0 695 747 850 2672 2885 3625 3738 Size: 695 52 103 1822 213 740 113 Fragments arranged by size: 1822 740 695 213 113 103 52 DraIII CAC_nnn'GTG Cuts at: 0 2748 3738 Size: 2748 990 EaeI y'GGCC_r Cuts at: 0 463 2406 3422 3719 3738 Size: 463 1943 1016 297 19 Fragments arranged by size: 1943 1016 463 297 19 EarI CTCTTCn'nnn_ Cuts at: 0 404 1783 1810 1954 2293 3738 Size: 404 1379 27 144 339 1445 Fragments arranged by size: 1445 1379 404 339 144 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2130 2221 3738 Size: 2130 91 1517 Fragments arranged by size: 2130 1517 91 EcoICRI GAG'CTC Cuts at: 0 69 112 1506 1878 3279 3738 Size: 69 43 1394 372 1401 459 Fragments arranged by size: 1401 1394 459 372 69 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 17 98 128 382 395 593 1270 1429 Size: 17 81 30 254 13 198 677 159 Cuts at: 1429 1480 1935 2527 2743 2747 2814 3034 3094 Size: 51 455 592 216 4 67 220 60 Cuts at: 3094 3738 Size: 644 Fragments arranged by size: 677 644 592 455 254 220 216 198 159 81 67 60 51 30 17 13 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 1966 3394 3738 Size: 1966 1428 344 Fragments arranged by size: 1966 1428 344 EcoO109I rG'GnC_Cy Cuts at: 0 706 819 1089 1245 1635 1636 1860 2070 Size: 706 113 270 156 390 1 224 210 Cuts at: 2070 2121 2352 2649 2731 3569 3579 3738 Size: 51 231 297 82 838 10 159 Fragments arranged by size: 838 706 390 297 270 231 224 210 159 156 113 82 51 10 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 565 2322 3738 Size: 565 1757 1416 Fragments arranged by size: 1757 1416 565 FspI TGC'GCA Cuts at: 0 2044 3738 Size: 2044 1694 HaeII r_GCGC'y Cuts at: 0 1818 3606 3738 Size: 1818 1788 132 Fragments arranged by size: 1818 1788 132 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 247 275 279 307 817 849 853 885 Size: 247 28 4 28 510 32 4 32 Cuts at: 885 3738 Size: 2853 Fragments arranged by size: 2853 510 247 32 32 28 28 4 4 HincII GTy'rAC Cuts at: 0 2418 3738 Size: 2418 1320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 323 469 623 628 3113 3738 Size: 323 146 154 5 2485 625 Fragments arranged by size: 2485 625 323 154 146 5 MscI TGG'CCA Cuts at: 0 465 2408 3424 3738 Size: 465 1943 1016 314 Fragments arranged by size: 1943 1016 465 314 MslI CAynn'nnrTG Cuts at: 0 856 2197 2791 3738 Size: 856 1341 594 947 Fragments arranged by size: 1341 947 856 594 MspA1I CmG'CkG Cuts at: 0 802 1164 1561 1652 1733 1851 2217 2438 Size: 802 362 397 91 81 118 366 221 Cuts at: 2438 3593 3685 3738 Size: 1155 92 53 Fragments arranged by size: 1155 802 397 366 362 221 118 92 91 81 53 NaeI GCC'GGC Cuts at: 0 406 3738 Size: 406 3332 NcoI C'CATG_G Cuts at: 0 132 1997 3738 Size: 132 1865 1741 Fragments arranged by size: 1865 1741 132 NgoMIV G'CCGG_C Cuts at: 0 404 3738 Size: 404 3334 NspI r_CATG'y Cuts at: 0 1872 2013 3540 3738 Size: 1872 141 1527 198 Fragments arranged by size: 1872 1527 198 141 PasI CC'CwG_GG Cuts at: 0 1497 2080 3192 3358 3738 Size: 1497 583 1112 166 380 Fragments arranged by size: 1497 1112 583 380 166 PciI A'CATG_T Cuts at: 0 1868 3738 Size: 1868 1870 PflMI CCAn_nnn'nTGG Cuts at: 0 339 616 2748 3471 3738 Size: 339 277 2132 723 267 Fragments arranged by size: 2132 723 339 277 267 PfoI T'CCnGG_A Cuts at: 0 615 1109 2066 2894 3738 Size: 615 494 957 828 844 Fragments arranged by size: 957 844 828 615 494 PmlI CAC'GTG Cuts at: 0 1690 3738 Size: 1690 2048 PpuMI rG'GwC_Cy Cuts at: 0 706 1245 1860 2070 2649 3569 3738 Size: 706 539 615 210 579 920 169 Fragments arranged by size: 920 706 615 579 539 210 169 PspOMI G'GGCC_C Cuts at: 0 1635 3002 3527 3738 Size: 1635 1367 525 211 Fragments arranged by size: 1635 1367 525 211 PstI C_TGCA'G Cuts at: 0 164 742 1008 1814 2222 2679 2966 3738 Size: 164 578 266 806 408 457 287 772 Fragments arranged by size: 806 772 578 457 408 287 266 164 PvuII CAG'CTG Cuts at: 0 802 1164 1561 1851 2217 2438 3738 Size: 802 362 397 290 366 221 1300 Fragments arranged by size: 1300 802 397 366 362 290 221 SacI G_AGCT'C Cuts at: 0 71 114 1508 1880 3281 3738 Size: 71 43 1394 372 1401 457 Fragments arranged by size: 1401 1394 457 372 71 43 SacII CC_GC'GG Cuts at: 0 1653 3738 Size: 1653 2085 SalI G'TCGA_C Cuts at: 0 2416 3738 Size: 2416 1322 SanDI GG'GwC_CC Cuts at: 0 3569 3738 Size: 3569 169 SapI GCTCTTCn'nnn_ Cuts at: 0 1783 1954 3738 Size: 1783 171 1784 Fragments arranged by size: 1784 1783 171 SexAI A'CCwGG_T Cuts at: 0 561 3738 Size: 561 3177 SfcI C'TryA_G Cuts at: 0 160 304 738 1004 1810 2218 2578 2675 Size: 160 144 434 266 806 408 360 97 Cuts at: 2675 2962 3738 Size: 287 776 Fragments arranged by size: 806 776 434 408 360 287 266 160 144 97 SfiI GGCCn_nnn'nGGCC Cuts at: 0 414 3738 Size: 414 3324 SmaI CCC'GGG Cuts at: 0 1726 3532 3738 Size: 1726 1806 206 Fragments arranged by size: 1806 1726 206 SmlI C'TyrA_G Cuts at: 0 1381 1927 3391 3738 Size: 1381 546 1464 347 Fragments arranged by size: 1464 1381 546 347 SphI G_CATG'C Cuts at: 0 3540 3738 Size: 3540 198 SspI AAT'ATT Cuts at: 0 2431 3738 Size: 2431 1307 StuI AGG'CCT Cuts at: 0 1177 1747 1754 3738 Size: 1177 570 7 1984 Fragments arranged by size: 1984 1177 570 7 StyI C'CwwG_G Cuts at: 0 132 1997 2789 3738 Size: 132 1865 792 949 Fragments arranged by size: 1865 949 792 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 335 649 1885 2939 3655 3738 Size: 335 314 1236 1054 716 83 Fragments arranged by size: 1236 1054 716 335 314 83 TatI w'GTAC_w Cuts at: 0 1158 1220 3042 3738 Size: 1158 62 1822 696 Fragments arranged by size: 1822 1158 696 62 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1237 1269 2041 2073 2516 2548 3644 3676 Size: 1237 32 772 32 443 32 1096 32 Cuts at: 3676 3738 Size: 62 Fragments arranged by size: 1237 1096 772 443 62 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 979 2950 3738 Size: 979 1971 788 Fragments arranged by size: 1971 979 788 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 914 960 3451 3738 Size: 914 46 2491 287 Fragments arranged by size: 2491 914 287 46 XmaI C'CCGG_G Cuts at: 0 1724 3530 3738 Size: 1724 1806 208 Fragments arranged by size: 1806 1724 208 Enzymes that do cut: AccI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI BciVI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII SalI SanDI SapI SexAI SfcI SfiI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AlfI AloI AscI AseI AsiSI AvrII BbeI BcgI BclI BfrBI BlpI BmgBI BmtI BsiEI BsiWI BsmBI BssHII BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI FspAI HindIII HpaI KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI XbaI XhoI XmnI ZraI