Restriction map for
pFN21AE1322
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII
BstPI Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HapII HhaI
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI MaeI MaeIII
MamI MfeI MluI MroI MseI
MspI MunI NaeI NarI NciI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tru9I Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 316 GACGTC GACGT^C
AccI 96 GTATAC GT^[AC][GT]AC
AccII 31 CGCG CG^CG
AcsI 148 AAATTC [GA]^AATT[CT]
ApoI 148 AAATTC [GA]^AATT[CT]
Asp700I 543 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 332 TGGCCA TGG^CCA
BbvI 405 GCAGC GCAGC(8/12)
BglII 578 AGATCT A^GATCT
BseRI 274 GAGGAG GAGGAG(10/8)
BsmBI 318 CGTCTC CGTCTC(1/5)
Bsp1407I 623 TGTACA T^GTACA
BsrDI 237 CATTGC GCAATG(2/0)
BsrGI 623 TGTACA T^GTACA
Bst1107I 96 GTATAC GTA^TAC
BstUI 31 CGCG CG^CG
BstYI 578 AGATCT [GA]^GATC[CT]
BstZ17I 96 GTATAC GTA^TAC
EaeI 332 TGGCCA [CT]^GGCC[GA]
EcoT14I 436 CCATGG C^C[AT][AT]GG
KspI 30 CCGCGG CCGC^GG
MaeII 317 ACGT A^CGT
MflI 578 AGATCT [GA]^GATC[CT]
MluNI 332 TGGCCA TGG^CCA
MscI 332 TGGCCA TGG^CCA
MslI 627 CACAAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 31 CGCG CG^CG
MwoI 560 GCTTTGAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 436 CCATGG C^CATGG
PflMI 306 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 328 CAGCTG CAG^CTG
SacII 30 CCGCGG CCGC^GG
SfaNI 402 GATGC GCATC(5/9)
SfcI 345 CTACAG C^T[GA][CT]AG
SmlI 75 CTCAAG C^T[CT][GA]AG
SspBI 623 TGTACA T^GTACA
StyI 436 CCATGG C^C[AT][AT]GG
TfiI 282 GATTC G^A[AT]TC
TseI 405 GCAGC G^C[AT]GC
Tsp509I 149 AATT ^AATT
Van91I 306 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 578 AGATCT [GA]^GATC[CT]
XmnI 543 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 316 GACGTC G[GA]^CG[CT]C
526 GACGCC
AfaI 18 GTAC GT^AC
624 GTAC
BanII 197 GGGCTC G[GA]GC[CT]^C
511 GAGCCC
BmyI 197 GGGCTC G[AGT]GC[ACT]^C
511 GAGCCC
BsaHI 316 GACGTC G[GA]^CG[CT]C
526 GACGCC
BsmFI 23 GGGAC GGGAC(10/14)
107 GGGAC
Bsp1286I 197 GGGCTC G[AGT]GC[ACT]^C
511 GAGCCC
Cfr13I 39 GGCCC G^G[ACGT]CC
159 GGGCC
DsaI 30 CCGCGG C^C[GA][CT]GG
436 CCATGG
EarI 271 GAAGAG CTCTTC(1/4)
429 GAAGAG
Fnu4HI 405 GCAGC GC^[ACGT]GC
602 GCCGC
HgaI 109 GACGC GACGC(5/10)
526 GACGC
Hin1I 316 GACGTC G[GA]^CG[CT]C
526 GACGCC
HphI 209 TCACC GGTGA(8/7)
458 TCACC
ItaI 405 GCAGC GC^[ACGT]GC
602 GCCGC
Ksp632I 271 GAAGAG CTCTTC(1/4)
429 GAAGAG
MspA1I 30 CCGCGG C[AC]G^C[GT]G
328 CAGCTG
PleI 73 GACTC GAGTC(4/5)
338 GAGTC
RsaI 18 GTAC GT^AC
624 GTAC
Sau96I 39 GGCCC G^G[ACGT]CC
159 GGGCC
TspRI 11 CACTG CA[GC]TG[ACGT][ACGT]^
133 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI AlwNI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI BstXI Cac8I DpnII EcoRII
FauI FokI HaeIII HinfI MboI
MboII MnlI MvaI NdeII NlaIII
Sau3AI ScrFI