(Circular) (Six-base) MAPSORT of: pFN21AE1322.seq Check: 3971 from: 1 to: 5359 pFN21AE1322 5359 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2325 3169 278 Size: 53 83 186 936 789 844 2468 Fragments arranged by size: 2468 936 844 789 186 83 53 AccI GT'mk_AC Cuts at: 1937 2102 2702 1937 Size: 165 600 4594 Fragments arranged by size: 4594 600 165 Acc65I G'GTAC_C Cuts at: 2680 2680 Size: 5359 AclI AA'CG_TT Cuts at: 1563 3486 3859 1563 Size: 1923 373 3063 Fragments arranged by size: 3063 1923 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2393 2567 3422 4806 5074 1632 Size: 761 174 855 1384 268 1917 Fragments arranged by size: 1917 1384 855 761 268 174 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5162 AflIII A'CryG_T Cuts at: 1203 4258 5235 1203 Size: 3055 977 1327 Fragments arranged by size: 3055 1327 977 AgeI A'CCGG_T Cuts at: 4190 4190 Size: 5359 AhdI GACnn_n'nnGTC Cuts at: 4088 4088 Size: 5359 AleI CACnn'nnGTG Cuts at: 1166 2636 1166 Size: 1470 3889 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5359 AlwNI CAG_nnn'CTG Cuts at: 2143 2346 2428 3102 4674 2143 Size: 203 82 674 1572 2828 Fragments arranged by size: 2828 1572 674 203 82 ApaLI G'TGCA_C Cuts at: 3416 4572 3416 Size: 1156 4203 ApoI r'AATT_y Cuts at: 1493 2153 2668 2894 3046 5155 1493 Size: 660 515 226 152 2109 1697 Fragments arranged by size: 2109 1697 660 515 226 152 AseI AT'TA_AT Cuts at: 160 1032 3913 160 Size: 872 2881 1606 Fragments arranged by size: 2881 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 AvaI C'yCGr_G Cuts at: 1870 1943 2684 1870 Size: 73 741 4545 Fragments arranged by size: 4545 741 73 BamHI G'GATC_C Cuts at: 2689 3056 2689 Size: 367 4992 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2680 4135 618 Size: 325 217 627 893 1455 1842 Fragments arranged by size: 1842 1455 893 627 325 217 BanII G_rGCy'C Cuts at: 1391 2206 2520 2678 1391 Size: 815 314 158 4072 Fragments arranged by size: 4072 815 314 158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4388 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3550 3584 1903 Size: 34 1613 34 3678 Fragments arranged by size: 3678 1613 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3251 4461 3251 Size: 1210 4149 BclI T'GATC_A Cuts at: 1548 1548 Size: 5359 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3970 136 Size: 107 122 71 631 605 2298 1525 Fragments arranged by size: 2298 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2583 5354 2583 Size: 2771 2588 BlpI GC'TnA_GC Cuts at: 2776 2776 Size: 5359 Bme1580I G_kGCm'C Cuts at: 1790 3420 4576 1790 Size: 1630 1156 2573 Fragments arranged by size: 2573 1630 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5359 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2027 2035 3090 Size: 416 53 83 399 24 610 8 1055 Cuts at: 3090 4048 442 Size: 958 1753 Fragments arranged by size: 1753 1055 958 610 416 399 83 53 24 8 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5359 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2602 2602 Size: 5359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4019 1239 Size: 2780 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2065 3484 4349 4647 2065 Size: 1419 865 298 2777 Fragments arranged by size: 2777 1419 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4022 882 Size: 617 2523 2219 Fragments arranged by size: 2523 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3150 493 Size: 711 1946 2702 Fragments arranged by size: 2702 1946 711 BsaBI GATnn'nnATC Cuts at: 5353 5353 Size: 5359 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2322 2532 3166 3548 Size: 53 83 186 936 789 210 634 382 Cuts at: 3548 4183 275 Size: 635 1451 Fragments arranged by size: 1451 936 789 635 634 382 210 186 83 53 BsaWI w'CCGG_w Cuts at: 3792 4190 4464 4611 5022 3792 Size: 398 274 147 411 4129 Fragments arranged by size: 4129 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2543 2573 1178 Size: 30 165 30 1140 30 3964 Fragments arranged by size: 3964 1140 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2294 1186 Size: 1108 4251 BseYI C'CCAG_C Cuts at: 2161 4562 5058 2161 Size: 2401 496 2462 Fragments arranged by size: 2462 2401 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4786 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2809 3570 4598 1540 Size: 275 186 808 761 1028 2301 Fragments arranged by size: 2301 1028 808 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3420 3505 4576 3420 Size: 85 1071 4203 Fragments arranged by size: 4203 1071 85 BsmI GAATG_Cn' Cuts at: 2870 2963 2870 Size: 93 5266 BsmBI CGTCTCn'nnnn_ Cuts at: 2329 2329 Size: 5359 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2206 2520 2678 3420 3505 4576 1391 Size: 399 416 314 158 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 416 399 314 158 85 BspEI T'CCGG_A Cuts at: 5022 5022 Size: 5359 BspHI T'CATG_A Cuts at: 3248 4978 3248 Size: 1730 3629 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2714 844 Size: 1870 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3246 1394 Size: 429 1423 3507 Fragments arranged by size: 3507 1423 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2241 3854 4028 65 Size: 1183 993 1613 174 1396 Fragments arranged by size: 1613 1396 1183 993 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4003 4190 1700 Size: 110 2193 187 2869 Fragments arranged by size: 2869 2193 187 110 BsrGI T'GTAC_A Cuts at: 96 2628 96 Size: 2532 2827 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5359 BssSI C'ACGA_G Cuts at: 3419 4431 3419 Size: 1012 4347 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5359 BstBI TT'CG_AA Cuts at: 4163 4163 Size: 5359 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5359 BstXI CCAn_nnnn'nTGG Cuts at: 2017 2335 2527 2017 Size: 318 192 4849 Fragments arranged by size: 4849 318 192 BstYI r'GATC_y Cuts at: 1972 2583 2689 3056 3441 3458 4910 4996 5008 Size: 611 106 367 385 17 1452 86 12 Cuts at: 5008 5354 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 611 385 367 346 106 86 17 12 BstZ17I GTA'TAC Cuts at: 2103 2103 Size: 5359 BtgI C'CryG_G Cuts at: 513 1065 2035 2441 513 Size: 552 970 406 3431 Fragments arranged by size: 3431 970 552 406 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4620 Fragments arranged by size: 4620 683 56 BtsI GCAGTG_nn' Cuts at: 2875 3669 3689 2875 Size: 794 20 4545 Fragments arranged by size: 4545 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5359 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2245 2280 529 Size: 35 1681 35 3608 Fragments arranged by size: 3608 1681 35 35 DraI TTT'AAA Cuts at: 2663 2663 Size: 5359 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4366 809 Size: 507 3050 1802 Fragments arranged by size: 3050 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2337 2806 3695 Size: 54 1425 50 275 18 507 469 889 Cuts at: 3695 5324 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 507 469 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2806 1537 Size: 275 994 4090 Fragments arranged by size: 4090 994 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2271 2429 3289 1353 Size: 918 158 860 3423 Fragments arranged by size: 3423 918 860 158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2045 3946 4320 4466 387 Size: 1064 138 456 1901 374 146 1280 Fragments arranged by size: 1901 1280 1064 456 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2393 2567 3422 4019 4806 5074 1239 Size: 393 761 174 855 597 787 268 1524 Fragments arranged by size: 1524 855 787 761 597 393 268 174 EcoRI G'AATT_C Cuts at: 2668 3046 2668 Size: 378 4981 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2482 2482 Size: 5359 FspI TGC'GCA Cuts at: 3865 3865 Size: 5359 HaeII r_GCGC'y Cuts at: 1678 4506 1678 Size: 2828 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2445 2477 2543 2575 4000 Size: 32 135 32 873 32 66 32 1425 Cuts at: 4000 4032 4074 4106 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1425 873 135 66 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2703 2949 669 Size: 988 281 765 246 3079 Fragments arranged by size: 3079 988 765 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5359 HpaI GTT'AAC Cuts at: 2949 2949 Size: 5359 KpnI G_GTAC'C Cuts at: 2684 2684 Size: 5359 MfeI C'AATT_G Cuts at: 2958 2958 Size: 5359 MluI A'CGCG_T Cuts at: 5235 5235 Size: 5359 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2431 4473 4657 1305 Size: 1126 2042 184 2007 Fragments arranged by size: 2042 2007 1126 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2339 10 Size: 54 1425 343 507 3030 Fragments arranged by size: 3030 1425 507 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2636 3317 3676 3835 518 Size: 648 843 627 681 359 159 2042 Fragments arranged by size: 2042 843 681 648 627 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 2335 2775 3452 4600 4845 1606 Size: 431 298 440 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 440 431 298 245 NcoI C'CATG_G Cuts at: 513 1065 2441 513 Size: 552 1376 3431 Fragments arranged by size: 3431 1376 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5359 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5359 NotI GC'GGCC_GC Cuts at: 2806 2806 Size: 5359 NspI r_CATG'y Cuts at: 1132 1229 2717 4262 1132 Size: 97 1488 1545 2229 Fragments arranged by size: 2229 1545 1488 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5359 PciI A'CATG_T Cuts at: 4258 4258 Size: 5359 PflMI CCAn_nnn'nTGG Cuts at: 1108 2317 1108 Size: 1209 4150 PfoI T'CCnGG_A Cuts at: 2064 2064 Size: 5359 PmeI GTTT'AAAC Cuts at: 2663 2663 Size: 5359 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3428 3460 4941 4973 3428 Size: 32 1481 32 3814 Fragments arranged by size: 3814 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5359 PsiI TTA'TAA Cuts at: 2929 2929 Size: 5359 PstI C_TGCA'G Cuts at: 830 2711 830 Size: 1881 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5359 PvuII CAG'CTG Cuts at: 2335 2335 Size: 5359 SacII CC_GC'GG Cuts at: 2038 2038 Size: 5359 SalI G'TCGA_C Cuts at: 1936 2701 1936 Size: 765 4594 SbfI CC_TGCA'GG Cuts at: 2711 2711 Size: 5359 ScaI AGT'ACT Cuts at: 3607 5319 3607 Size: 1712 3647 SfcI C'TryA_G Cuts at: 826 1045 2350 2707 3842 4523 4714 826 Size: 219 1305 357 1135 681 191 1471 Fragments arranged by size: 1471 1305 1135 681 357 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5359 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5359 SmaI CCC'GGG Cuts at: 1872 2686 1872 Size: 814 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2080 3463 4364 4626 820 Size: 197 926 137 1383 901 262 1553 Fragments arranged by size: 1553 1383 926 901 262 197 137 SnaBI TAC'GTA Cuts at: 493 3150 493 Size: 2657 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5359 SphI G_CATG'C Cuts at: 1132 2717 1132 Size: 1585 3774 SspI AAT'ATT Cuts at: 5 52 3283 5 Size: 47 3231 2081 Fragments arranged by size: 3231 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5359 StyI C'CwwG_G Cuts at: 513 1065 2441 2798 513 Size: 552 1376 357 3074 Fragments arranged by size: 3074 1376 552 357 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 3386 3403 3556 3741 4186 325 Size: 1255 651 1155 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1155 651 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2628 3605 5317 Size: 274 80 33 51 1443 651 977 1712 Cuts at: 5317 96 Size: 138 Fragments arranged by size: 1712 1443 977 651 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5319 5351 5319 Size: 32 5327 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5359 XbaI T'CTAG_A Cuts at: 2695 2695 Size: 5359 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5359 XmaI C'CCGG_G Cuts at: 1870 2684 1870 Size: 814 4545 XmnI GAAnn'nnTTC Cuts at: 2552 3488 2552 Size: 936 4423 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2323 3167 276 Size: 53 83 186 936 789 844 2468 Fragments arranged by size: 2468 936 844 789 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI