(Linear) (Six-base) MAPSORT of: KIEE1322.seq Check: 1020 from: 1 to: 654 KIEE1322.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AatII G_ACGT'C Cuts at: 0 320 654 Size: 320 334 AccI GT'mk_AC Cuts at: 0 97 654 Size: 97 557 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 562 654 Size: 388 174 92 Fragments arranged by size: 388 174 92 AleI CACnn'nnGTG Cuts at: 0 631 654 Size: 631 23 AlwNI CAG_nnn'CTG Cuts at: 0 138 341 423 654 Size: 138 203 82 231 Fragments arranged by size: 231 203 138 82 ApoI r'AATT_y Cuts at: 0 148 654 Size: 148 506 BanII G_rGCy'C Cuts at: 0 201 515 654 Size: 201 314 139 Fragments arranged by size: 314 201 139 BglII A'GATC_T Cuts at: 0 578 654 Size: 578 76 BmrI ACTGGGnnnn_n' Cuts at: 0 22 30 654 Size: 22 8 624 Fragments arranged by size: 624 22 8 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 597 654 Size: 597 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 654 Size: 60 594 BsaHI Gr'CG_yC Cuts at: 0 317 527 654 Size: 317 210 127 Fragments arranged by size: 317 210 127 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 538 568 654 Size: 538 30 86 Fragments arranged by size: 538 86 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 289 654 Size: 289 365 BseYI C'CCAG_C Cuts at: 0 156 654 Size: 156 498 BsmBI CGTCTCn'nnnn_ Cuts at: 0 324 654 Size: 324 330 Bsp1286I G_dGCh'C Cuts at: 0 201 515 654 Size: 201 314 139 Fragments arranged by size: 314 201 139 BsrDI GCAATG_nn' Cuts at: 0 236 654 Size: 236 418 BsrGI T'GTAC_A Cuts at: 0 623 654 Size: 623 31 BstXI CCAn_nnnn'nTGG Cuts at: 0 12 330 522 654 Size: 12 318 192 132 Fragments arranged by size: 318 192 132 12 BstYI r'GATC_y Cuts at: 0 578 654 Size: 578 76 BstZ17I GTA'TAC Cuts at: 0 98 654 Size: 98 556 BtgI C'CryG_G Cuts at: 0 30 436 654 Size: 30 406 218 Fragments arranged by size: 406 218 30 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 240 275 654 Size: 240 35 379 Fragments arranged by size: 379 240 35 EaeI y'GGCC_r Cuts at: 0 332 654 Size: 332 322 EarI CTCTTCn'nnn_ Cuts at: 0 266 424 654 Size: 266 158 230 Fragments arranged by size: 266 230 158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 40 654 Size: 40 614 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 562 654 Size: 388 174 92 Fragments arranged by size: 388 174 92 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 477 654 Size: 477 177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 440 472 538 570 654 Size: 440 32 66 32 84 Fragments arranged by size: 440 84 66 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 426 654 Size: 426 228 MscI TGG'CCA Cuts at: 0 334 654 Size: 334 320 MslI CAynn'nnrTG Cuts at: 0 631 654 Size: 631 23 MspA1I CmG'CkG Cuts at: 0 32 330 654 Size: 32 298 324 Fragments arranged by size: 324 298 32 NcoI C'CATG_G Cuts at: 0 436 654 Size: 436 218 PflMI CCAn_nnn'nTGG Cuts at: 0 312 654 Size: 312 342 PfoI T'CCnGG_A Cuts at: 0 59 654 Size: 59 595 PvuII CAG'CTG Cuts at: 0 330 654 Size: 330 324 SacII CC_GC'GG Cuts at: 0 33 654 Size: 33 621 SfcI C'TryA_G Cuts at: 0 345 654 Size: 345 309 SmlI C'TyrA_G Cuts at: 0 75 654 Size: 75 579 StyI C'CwwG_G Cuts at: 0 436 654 Size: 436 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 226 654 Size: 226 428 TatI w'GTAC_w Cuts at: 0 623 654 Size: 623 31 XmnI GAAnn'nnTTC Cuts at: 0 547 654 Size: 547 107 ZraI GAC'GTC Cuts at: 0 318 654 Size: 318 336 Enzymes that do cut: AatII AccI AcuI AleI AlwNI ApoI BanII BglII BmrI BplI BpuEI BsaHI BsaXI BseRI BseYI BsmBI Bsp1286I BsrDI BsrGI BstXI BstYI BstZ17I BtgI CspCI EaeI EarI EciI Eco57MI FalI Hin4I MmeI MscI MslI MspA1I NcoI PflMI PfoI PvuII SacII SfcI SmlI StyI TaqII TatI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI