Restriction map for
pFN21AE1542
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AluI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI KspI
MaeIII MamI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI StuI
SwaI Tsp45I Tth111I Van91I XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AciI 420 CCGC CCGC(-3/-1)
AcsI 277 GAATTC [GA]^AATT[CT]
AfaI 178 GTAC GT^AC
AflIII 466 ACGTGT A^C[GA][CT]GT
AlwI 132 GATCC GGATC(4/5)
ApoI 277 GAATTC [GA]^AATT[CT]
Asp700I 220 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 425 CTCGAG C^[CT]CG[GA]G
BbrPI 465 CACGTG CAC^GTG
BbsI 316 GAAGAC GAAGAC(2/6)
BfaI 61 CTAG C^TAG
BglII 124 AGATCT A^GATCT
BpuAI 316 GAAGAC GAAGAC(2/6)
BsaAI 465 CACGTG [CT]AC^GT[GA]
BsaI 206 GGTCTC GGTCTC(1/5)
BseRI 286 GAGGAG GAGGAG(10/8)
BsiYI 135 CCCACATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 135 CCCACATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 207 GTCTC GTCTC(1/5)
BsmI 384 GCATTC GAATGC(1/-1)
BsoBI 425 CTCGAG C^[CT]CG[GA]G
BspMI 566 ACCTGC ACCTGC(4/8)
BsrDI 201 GCAATG GCAATG(2/0)
BsrI 241 ACTGG ACTGG(1/-1)
BstF5I 174 GGATG GGATG(2/0)
BstYI 124 AGATCT [GA]^GATC[CT]
DdeI 121 CTGAG C^T[ACGT]AG
EarI 361 GAAGAG CTCTTC(1/4)
EcoRI 277 GAATTC G^AATTC
EcoT14I 170 CCAAGG C^C[AT][AT]GG
FokI 174 GGATG GGATG(9/13)
HaeIII 86 GGCC GG^CC
Ksp632I 361 GAAGAG CTCTTC(1/4)
MaeI 61 CTAG C^TAG
MaeII 466 ACGT A^CGT
MflI 124 AGATCT [GA]^GATC[CT]
MslI 385 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 425 CTCGAG C^TCGAG
PmaCI 465 CACGTG CAC^GTG
PmlI 465 CACGTG CAC^GTG
PstI 568 CTGCAG CTGCA^G
RsaI 178 GTAC GT^AC
SbfI 567 CCTGCAGG CCTGCA^GG
SfaNI 391 GATGC GCATC(5/9)
SmlI 425 CTCGAG C^T[CT][GA]AG
Sse8387I 567 CCTGCAGG CCTGCA^GG
StyI 170 CCAAGG C^C[AT][AT]GG
TaqI 426 TCGA T^CGA
TspRI 282 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 426 TCGA T^CGA
XbaI 60 TCTAGA T^CTAGA
XhoI 425 CTCGAG C^TCGAG
XhoII 124 AGATCT [GA]^GATC[CT]
XmnI 220 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwNI 332 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
389 CAGATGCTG
BbvI 187 GCTGC GCAGC(8/12)
394 GCTGC
BsaJI 170 CCAAGG C^C[ACGT][ACGT]GG
523 CCCAGG
BssKI 4 CCAGG ^CC[ACGT]GG
524 CCAGG
BstNI 4 CCAGG CC^[AT]GG
524 CCAGG
Cac8I 306 GCTGGC GC[ACGT]^[ACGT]GC
570 GCAGGC
EcoRII 4 CCAGG ^CC[AT]GG
524 CCAGG
Fnu4HI 187 GCTGC GC^[ACGT]GC
394 GCTGC
ItaI 187 GCTGC GC^[ACGT]GC
394 GCTGC
MseI 449 TTAA T^TAA
550 TTAA
MvaI 4 CCAGG CC^[AT]GG
524 CCAGG
NlaIV 43 GGAACC GG[ACGT]^[ACGT]CC
527 GGTTCC
PleI 514 GACTC GAGTC(4/5)
334 GAGTC
ScrFI 4 CCAGG CC^[ACGT]GG
524 CCAGG
SfcI 434 CTACAG C^T[GA][CT]AG
568 CTGCAG
SspI 292 AATATT AAT^ATT
409 AATATT
TfiI 145 GAATC G^A[AT]TC
430 GAATC
Tru9I 449 TTAA T^TAA
550 TTAA
TseI 187 GCTGC G^C[AT]GC
394 GCTGC
Tsp509I 35 AATT ^AATT
278 AATT
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# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
DpnII HinfI MboI MboII MnlI
NdeII Sau3AI