Restriction map for
pFN21AE5513
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII Hin1I
HindIII KasI KpnI KspI MfeI
MluI MroI MspA1I MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AclI 91 AACGTT AA^CGTT
AcsI 756 AAATTT [GA]^AATT[CT]
ApoI 756 AAATTT [GA]^AATT[CT]
Asp700I 75 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1047 CCCGGG C^[CT]CG[GA]G
BalI 131 TGGCCA TGG^CCA
BanI 1027 GGCACC G^G[CT][GA]CC
BanII 1080 GGGCTC G[GA]GC[CT]^C
BcgI 425 GCAGTGTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 728 CTAG C^TAG
BsaBI 100 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1195 GAGGAG GAGGAG(10/8)
BsgI 1021 CTGCAC GTGCAG(16/14)
BsmFI 450 GGGAC GGGAC(10/14)
BsoBI 1047 CCCGGG C^[CT]CG[GA]G
BstF5I 867 CATCC GGATG(2/0)
CfoI 455 GCGC GCG^C
DraII 851 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 631 GACTTTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 131 TGGCCA [CT]^GGCC[GA]
EarI 882 CTCTTC CTCTTC(1/4)
EcoNI 465 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 851 AGGCCCT [GA]G^G[ACGT]CC[CT]
FokI 867 CATCC GGATG(9/13)
HgaI 452 GACGC GACGC(5/10)
HhaI 455 GCGC GCG^C
HinP1I 455 GCGC G^CGC
HpaI 88 GTTAAC GTT^AAC
Ksp632I 882 CTCTTC CTCTTC(1/4)
MaeI 728 CTAG C^TAG
MamI 100 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 131 TGGCCA TGG^CCA
MscI 131 TGGCCA TGG^CCA
PflMI 530 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 91 AACGTT AA^CGTT
SmaI 1047 CCCGGG CCC^GGG
SspI 655 AATATT AAT^ATT
Van91I 530 CCAGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1226 CCAGCTACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1047 CCCGGG C^CCGGG
XmaI 1047 CCCGGG C^CCGGG
XmnI 75 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 124 CGCG CG^CG
454 CGCG
AlwNI 164 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
830 CAGAGTCTG
AspHI 58 GAGCAC G[AT]GC[AT]^C
649 GTGCTC
AvaII 680 GGACC G^G[AT]CC
820 GGACC
BbvI 731 GCTGC GCAGC(8/12)
1072 GCTGC
BsaI 844 GAGACC GGTCTC(1/5)
1198 GAGACC
BsiHKAI 58 GAGCAC G[AT]GC[AT]^C
649 GTGCTC
BsmAI 844 GAGAC GTCTC(1/5)
1198 GAGAC
BsmI 981 GAATGC GAATGC(1/-1)
550 GCATTC
BspMI 822 ACCTGC ACCTGC(4/8)
1189 ACCTGC
BstUI 124 CGCG CG^CG
454 CGCG
BstXI 503 CCATTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
848 CCAAGGCCCTGG
EcoT14I 848 CCAAGG C^C[AT][AT]GG
1104 CCAAGG
HincII 88 GTTAAC GT[CT]^[GA]AC
199 GTTGAC
HindII 88 GTTAAC GT[CT]^[GA]AC
199 GTTGAC
MvnI 124 CGCG CG^CG
454 CGCG
MwoI 731 GCTGCATTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1148 GCAGATATGGC
NdeI 944 CATATG CA^TATG
1040 CATATG
SfaNI 763 GATGC GCATC(5/9)
37 GCATC
StyI 848 CCAAGG C^C[AT][AT]GG
1104 CCAAGG
TseI 731 GCTGC G^C[AT]GC
1072 GCTGC
Tsp509I 757 AATT ^AATT
780 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
TaqI TfiI Tru9I Tsp45I TspRI
TthHB8I XhoII