(Circular) (Six-base) MAPSORT of: pFN21AE5513.seq Check: 9586 from: 1 to: 5956 pFN21AE5513 5956 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 2097 4083 4456 1563 Size: 534 1986 373 3063 Fragments arranged by size: 3063 1986 534 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2495 4019 5403 5671 1632 Size: 863 1524 1384 268 1917 Fragments arranged by size: 1917 1524 1384 863 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 4855 5832 1203 Size: 3652 977 1327 Fragments arranged by size: 3652 1327 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5956 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AlwNI CAG_nnn'CTG Cuts at: 2174 2840 3699 5271 2174 Size: 666 859 1572 2859 Fragments arranged by size: 2859 1572 859 666 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 2761 3265 3491 3643 5752 1493 Size: 1268 504 226 152 2109 1697 Fragments arranged by size: 2109 1697 1268 504 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3052 3281 1870 Size: 73 1109 229 4545 Fragments arranged by size: 4545 1109 229 73 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3032 3277 4732 618 Size: 325 217 627 1245 245 1455 1842 Fragments arranged by size: 1842 1455 1245 627 325 245 217 BanII G_rGCy'C Cuts at: 1391 3089 3275 1391 Size: 1698 186 4072 Fragments arranged by size: 4072 1698 186 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4985 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2419 2453 4147 4181 1903 Size: 34 482 34 1694 34 3678 Fragments arranged by size: 3678 1694 482 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4567 136 Size: 107 122 71 631 605 337 2558 1525 Fragments arranged by size: 2558 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 4017 5173 1790 Size: 2227 1156 2573 Fragments arranged by size: 2573 2227 1156 BmgBI CAC'GTC Cuts at: 1318 2600 1318 Size: 1282 4674 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3106 3687 4645 Size: 416 53 83 399 24 1689 581 958 Cuts at: 4645 442 Size: 1753 Fragments arranged by size: 1753 1689 958 581 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2727 2727 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4616 1239 Size: 3377 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2843 3197 4619 882 Size: 617 1344 354 1422 2219 Fragments arranged by size: 2219 1422 1344 617 354 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 2109 5950 2109 Size: 3841 2115 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3763 4145 4780 275 Size: 53 83 186 936 2230 382 635 1451 Fragments arranged by size: 2230 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2300 2330 2906 2936 1178 Size: 30 165 30 897 30 576 30 4198 Fragments arranged by size: 4198 897 576 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3215 1186 Size: 2029 3927 BseYI C'CCAG_C Cuts at: 3080 5159 5655 3080 Size: 2079 496 3381 Fragments arranged by size: 3381 2079 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3011 1163 Size: 573 1275 4108 Fragments arranged by size: 4108 1275 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 2658 4017 4102 5173 2067 Size: 591 1359 85 1071 2850 Fragments arranged by size: 2850 1359 1071 591 85 BsmI GAATG_Cn' Cuts at: 2555 2992 3467 3560 2555 Size: 437 475 93 4951 Fragments arranged by size: 4951 475 437 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2658 3089 3275 4017 4102 5173 Size: 399 277 591 431 186 742 85 1071 Cuts at: 5173 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 591 431 399 277 186 85 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2836 3203 3311 844 Size: 1992 367 108 3489 Fragments arranged by size: 3489 1992 367 108 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4451 4625 65 Size: 1183 3203 174 1396 Fragments arranged by size: 3203 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4600 4787 1700 Size: 110 2790 187 2869 Fragments arranged by size: 2869 2790 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 4016 5028 4016 Size: 1012 4944 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstXI CCAn_nnnn'nTGG Cuts at: 2515 2860 2515 Size: 345 5611 BstYI r'GATC_y Cuts at: 1972 2604 2729 3183 3286 3653 4038 4055 5507 Size: 632 125 454 103 367 385 17 1452 Cuts at: 5507 5593 5605 5951 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 632 454 385 367 346 125 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5404 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5217 Fragments arranged by size: 5217 683 56 BtsI GCAGTG_nn' Cuts at: 2437 3023 3472 4266 4286 2437 Size: 586 449 794 20 4107 Fragments arranged by size: 4107 794 586 449 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 3260 3260 Size: 5956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2642 4963 809 Size: 507 1326 2321 1802 Fragments arranged by size: 2321 1802 1326 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 3403 4292 Size: 54 1425 50 275 18 306 1267 889 Cuts at: 4292 5921 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1267 889 306 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2893 3886 1353 Size: 1540 993 3423 Fragments arranged by size: 3423 1540 993 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2495 4019 4616 5403 5671 1239 Size: 393 863 1524 597 787 268 1524 Fragments arranged by size: 1524 1524 863 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2474 2474 Size: 5956 EcoO109I rG'GnC_Cy Cuts at: 2857 2857 Size: 5956 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2653 2653 Size: 5956 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 5103 1678 Size: 3425 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2589 2621 4597 4629 4671 Size: 32 135 32 1017 32 1976 32 42 Cuts at: 4671 4703 1373 Size: 32 2626 Fragments arranged by size: 2626 1976 1017 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2095 2206 3300 3546 669 Size: 988 281 157 111 1094 246 3079 Fragments arranged by size: 3079 1094 988 281 246 157 111 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 2095 3546 2095 Size: 1451 4505 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2138 10 Size: 54 1425 343 306 3828 Fragments arranged by size: 3828 1425 343 306 54 MslI CAynn'nnrTG Cuts at: 518 1166 2055 2058 2349 2513 3914 4273 4432 Size: 648 889 3 291 164 1401 359 159 Cuts at: 4432 518 Size: 2042 Fragments arranged by size: 2042 1401 889 648 359 291 164 159 3 MspA1I CmG'CkG Cuts at: 1606 3372 4049 5197 5442 1606 Size: 1766 677 1148 245 2120 Fragments arranged by size: 2120 1766 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5404 NdeI CA'TA_TG Cuts at: 387 2950 3046 387 Size: 2563 96 3297 Fragments arranged by size: 3297 2563 96 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 3314 4859 1132 Size: 97 2085 1545 2229 Fragments arranged by size: 2229 2085 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 2541 1108 Size: 1433 4523 PfoI T'CCnGG_A Cuts at: 3240 3240 Size: 5956 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2069 2101 2568 2600 4025 4057 5538 5570 2069 Size: 32 467 32 1425 32 1481 32 2455 Fragments arranged by size: 2455 1481 1425 467 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PstI C_TGCA'G Cuts at: 830 3308 830 Size: 2478 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SfcI C'TryA_G Cuts at: 826 1045 3304 4439 5120 5311 826 Size: 219 2259 1135 681 191 1471 Fragments arranged by size: 2259 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3054 3283 1872 Size: 1182 229 4545 Fragments arranged by size: 4545 1182 229 SmlI C'TyrA_G Cuts at: 820 1017 1943 4060 4961 5223 820 Size: 197 926 2117 901 262 1553 Fragments arranged by size: 2117 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3314 1132 Size: 2182 3774 SspI AAT'ATT Cuts at: 5 52 2662 3880 5 Size: 47 2610 1218 2081 Fragments arranged by size: 2610 2081 1218 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2853 3109 3395 513 Size: 552 1788 256 286 3074 Fragments arranged by size: 3074 1788 552 286 256 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2735 3983 4000 4153 4338 4783 325 Size: 1255 1155 1248 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1248 1155 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2198 2358 3039 Size: 274 80 33 51 1443 221 160 681 Cuts at: 3039 4202 5914 96 Size: 1163 1712 138 Fragments arranged by size: 1712 1443 1163 681 274 221 160 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2395 2427 2906 2938 5916 5948 2395 Size: 32 479 32 2978 32 2403 Fragments arranged by size: 2978 2403 479 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3238 3238 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3052 3281 1870 Size: 1182 229 4545 Fragments arranged by size: 4545 1182 229 XmnI GAAnn'nnTTC Cuts at: 2084 4085 2084 Size: 2001 3955 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI