(Linear) (Six-base) MAPSORT of: KIEE5513.seq Check: 9991 from: 1 to: 1251 KIEE5513.orf With 182 enzymes: SgfI * January 29, 2014 12:03 .. AclI AA'CG_TT Cuts at: 0 92 1251 Size: 92 1159 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 490 1251 Size: 490 761 AlwNI CAG_nnn'CTG Cuts at: 0 169 835 1251 Size: 169 666 416 Fragments arranged by size: 666 416 169 ApoI r'AATT_y Cuts at: 0 756 1251 Size: 756 495 AvaI C'yCGr_G Cuts at: 0 1047 1251 Size: 1047 204 BanI G'GyrC_C Cuts at: 0 1027 1251 Size: 1027 224 BanII G_rGCy'C Cuts at: 0 1084 1251 Size: 1084 167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 414 448 1251 Size: 414 34 803 Fragments arranged by size: 803 414 34 BmgBI CAC'GTC Cuts at: 0 595 1251 Size: 595 656 BmrI ACTGGGnnnn_n' Cuts at: 0 1101 1251 Size: 1101 150 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 722 1251 Size: 722 529 BsaI GGTCTCn'nnnn_ Cuts at: 0 838 1192 1251 Size: 838 354 59 Fragments arranged by size: 838 354 59 BsaBI GATnn'nnATC Cuts at: 0 104 1251 Size: 104 1147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 295 325 901 931 1251 Size: 295 30 576 30 320 Fragments arranged by size: 576 320 295 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1210 1251 Size: 1210 41 BseYI C'CCAG_C Cuts at: 0 1075 1251 Size: 1075 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1006 1251 Size: 1006 245 BsiHKAI G_wGCw'C Cuts at: 0 62 653 1251 Size: 62 591 598 Fragments arranged by size: 598 591 62 BsmI GAATG_Cn' Cuts at: 0 550 987 1251 Size: 550 437 264 Fragments arranged by size: 550 437 264 Bsp1286I G_dGCh'C Cuts at: 0 62 653 1084 1251 Size: 62 591 431 167 Fragments arranged by size: 591 431 167 62 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 831 1198 1251 Size: 831 367 53 Fragments arranged by size: 831 367 53 BstXI CCAn_nnnn'nTGG Cuts at: 0 510 855 1251 Size: 510 345 396 Fragments arranged by size: 510 396 345 BstYI r'GATC_y Cuts at: 0 599 724 1178 1251 Size: 599 125 454 73 Fragments arranged by size: 599 454 125 73 BtsI GCAGTG_nn' Cuts at: 0 432 1018 1251 Size: 432 586 233 Fragments arranged by size: 586 432 233 DrdI GACnn_nn'nnGTC Cuts at: 0 637 1251 Size: 637 614 EaeI y'GGCC_r Cuts at: 0 131 1251 Size: 131 1120 EarI CTCTTCn'nnn_ Cuts at: 0 888 1251 Size: 888 363 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 490 1251 Size: 490 761 EcoNI CCTnn'n_nnAGG Cuts at: 0 469 1251 Size: 469 782 EcoO109I rG'GnC_Cy Cuts at: 0 852 1251 Size: 852 399 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 648 1251 Size: 648 603 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 584 616 1251 Size: 584 32 635 Fragments arranged by size: 635 584 32 HincII GTy'rAC Cuts at: 0 90 201 1251 Size: 90 111 1050 Fragments arranged by size: 1050 111 90 HpaI GTT'AAC Cuts at: 0 90 1251 Size: 90 1161 MscI TGG'CCA Cuts at: 0 133 1251 Size: 133 1118 MslI CAynn'nnrTG Cuts at: 0 50 53 344 508 1251 Size: 50 3 291 164 743 Fragments arranged by size: 743 291 164 50 3 NdeI CA'TA_TG Cuts at: 0 945 1041 1251 Size: 945 96 210 Fragments arranged by size: 945 210 96 PflMI CCAn_nnn'nTGG Cuts at: 0 536 1251 Size: 536 715 PfoI T'CCnGG_A Cuts at: 0 1235 1251 Size: 1235 16 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 64 96 563 595 1251 Size: 64 32 467 32 656 Fragments arranged by size: 656 467 64 32 32 SmaI CCC'GGG Cuts at: 0 1049 1251 Size: 1049 202 SspI AAT'ATT Cuts at: 0 657 1251 Size: 657 594 StyI C'CwwG_G Cuts at: 0 848 1104 1251 Size: 848 256 147 Fragments arranged by size: 848 256 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 730 1251 Size: 730 521 TatI w'GTAC_w Cuts at: 0 193 353 1034 1251 Size: 193 160 681 217 Fragments arranged by size: 681 217 193 160 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 390 422 901 933 1251 Size: 390 32 479 32 318 Fragments arranged by size: 479 390 318 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1233 1251 Size: 1233 18 XmaI C'CCGG_G Cuts at: 0 1047 1251 Size: 1047 204 XmnI GAAnn'nnTTC Cuts at: 0 79 1251 Size: 79 1172 Enzymes that do cut: AclI AcuI AlwNI ApoI AvaI BanI BanII BcgI BmgBI BmrI BplI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BstXI BstYI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I FalI Hin4I HincII HpaI MscI MslI NdeI PflMI PfoI PpiI SmaI SspI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmtI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HindIII KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI ZraI