Restriction map for
pFN21AE5219
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AclI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BciVI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeII MfeI MflI
MluI MluNI MscI MslI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SacII
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I Van91I XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 607 GTCGAC GT^[AC][GT]AC
AccII 333 CGCG CG^CG
AccIII 431 TCCGGA T^CCGGA
AluI 140 AGCT AG^CT
Asp700I 575 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 470 TGATCA T^GATCA
BcnI 81 CCGGG CC^[GC]GG
BfaI 220 CTAG C^TAG
BglI 339 GCCCGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 174 CTGGAG CTGGAG(16/14)
BsaBI 354 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 431 TCCGGA [AT]^CCGG[AT]
BseAI 431 TCCGGA T^CCGGA
BsgI 88 CTGCAC GTGCAG(16/14)
BsmFI 181 GTCCC GGGAC(10/14)
BspEI 431 TCCGGA T^CCGGA
BstF5I 213 CATCC GGATG(2/0)
BstUI 333 CGCG CG^CG
DraIII 9 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 5 CGGCCA [CT]^GGCC[GA]
EcoT14I 290 CCAAGG C^C[AT][AT]GG
FauI 64 CCCGC CCCGC(4/6)
FbaI 470 TGATCA T^GATCA
FokI 213 CATCC GGATG(9/13)
HaeII 84 GGCGCT [GA]GCGC^[CT]
HincII 607 GTCGAC GT[CT]^[GA]AC
HindII 607 GTCGAC GT[CT]^[GA]AC
HphI 404 TCACC GGTGA(8/7)
MaeI 220 CTAG C^TAG
MaeIII 209 GTGAC ^GT[ACGT]AC
MamI 354 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 431 TCCGGA T^CCGGA
MseI 518 TTAA T^TAA
MvnI 333 CGCG CG^CG
NciI 81 CCGGG CC^[GC]GG
PleI 44 GAGTC GAGTC(4/5)
PpuMI 179 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 451 CTGCAG CTGCA^G
SalI 607 GTCGAC G^TCGAC
SexAI 10 ACCAGGT A^CC[AT]GGT
SfaNI 122 GCATC GCATC(5/9)
StyI 290 CCAAGG C^C[AT][AT]GG
Tru9I 518 TTAA T^TAA
Tsp45I 209 GTGAC ^GT[GC]AC
XbaI 219 TCTAGA T^CTAGA
XmnI 575 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwI 399 GATCC GGATC(4/5)
329 GGATC
AvaII 180 GGTCC G^G[AT]CC
256 GGTCC
BanI 14 GGTGCC G^G[CT][GA]CC
71 GGTGCC
BanII 159 GGGCTC G[GA]GC[CT]^C
337 GAGCCC
BcgI 453 GCAGAAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
599 GCAGGAGGGTCG
BsaJI 259 CCTGGG C^C[ACGT][ACGT]GG
290 CCAAGG
BsmAI 464 GAGAC GTCTC(1/5)
564 GAGAC
CfoI 21 GCGC GCG^C
85 GCGC
DraII 26 GGGGCCT [GA]G^G[ACGT]CC[CT]
179 GGGTCCC
EcoO109I 26 GGGGCCT [GA]G^G[ACGT]CC[CT]
179 GGGTCCC
EcoRI 285 GAATTC G^AATTC
575 GAATTC
HhaI 21 GCGC GCG^C
85 GCGC
HinP1I 21 GCGC G^CGC
85 GCGC
MboII 351 GAAGA GAAGA(8/7)
511 GAAGA
SfcI 126 CTACAG C^T[GA][CT]AG
451 CTGCAG
TaqI 462 TCGA T^CGA
608 TCGA
TthHB8I 462 TCGA T^CGA
608 TCGA
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
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AciI AcsI ApoI BbvI BmyI
BsiYI BslI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MboI MnlI MspI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TfiI TseI Tsp509I
TspRI