(Circular) (Six-base) MAPSORT of: pFN21AE5219.seq Check: 6408 from: 1 to: 5323 pFN21AE5219 5323 bp With 182 enzymes: SgfI * January 30, 2014 17:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3133 278 Size: 53 83 186 936 1597 2468 Fragments arranged by size: 2468 1597 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2613 2666 1937 Size: 676 53 4594 Fragments arranged by size: 4594 676 53 Acc65I G'GTAC_C Cuts at: 2644 2644 Size: 5323 AclI AA'CG_TT Cuts at: 1563 3450 3823 1563 Size: 1887 373 3063 Fragments arranged by size: 3063 1887 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3386 4770 5038 1632 Size: 1754 1384 268 1917 Fragments arranged by size: 1917 1754 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5126 AflIII A'CryG_T Cuts at: 1203 4222 5199 1203 Size: 3019 977 1327 Fragments arranged by size: 3019 1327 977 AgeI A'CCGG_T Cuts at: 4154 4154 Size: 5323 AhdI GACnn_n'nnGTC Cuts at: 4052 4052 Size: 5323 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5323 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5323 AlwNI CAG_nnn'CTG Cuts at: 3066 4638 3066 Size: 1572 3751 ApaLI G'TGCA_C Cuts at: 3380 4536 3380 Size: 1156 4167 ApoI r'AATT_y Cuts at: 1493 2290 2463 2580 2632 2858 3010 5119 1493 Size: 797 173 117 52 226 152 2109 1697 Fragments arranged by size: 2109 1697 797 226 173 152 117 52 AseI AT'TA_AT Cuts at: 160 1032 3877 160 Size: 872 2845 1606 Fragments arranged by size: 2845 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5323 AvaI C'yCGr_G Cuts at: 1870 1943 2648 1870 Size: 73 705 4545 Fragments arranged by size: 4545 705 73 BamHI G'GATC_C Cuts at: 2653 3020 2653 Size: 367 4956 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2019 2076 2644 4099 618 Size: 325 217 627 232 57 568 1455 1842 Fragments arranged by size: 1842 1455 627 568 325 232 217 57 BanII G_rGCy'C Cuts at: 1391 2168 2346 2642 1391 Size: 777 178 296 4072 Fragments arranged by size: 4072 777 296 178 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4352 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2447 2481 2593 2627 3514 3548 1903 Size: 34 510 34 112 34 887 34 3678 Fragments arranged by size: 3678 887 510 112 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3215 4425 3215 Size: 1210 4113 BclI T'GATC_A Cuts at: 1548 2475 1548 Size: 927 4396 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2350 3934 Size: 107 122 71 631 605 337 341 1584 Cuts at: 3934 136 Size: 1525 Fragments arranged by size: 1584 1525 631 605 341 337 122 107 71 BglII A'GATC_T Cuts at: 5318 5318 Size: 5323 BlpI GC'TnA_GC Cuts at: 2740 2740 Size: 5323 Bme1580I G_kGCm'C Cuts at: 1790 2081 3384 4540 1790 Size: 291 1303 1156 2573 Fragments arranged by size: 2573 1303 1156 291 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5323 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3054 4012 442 Size: 416 53 83 399 24 1637 958 1753 Fragments arranged by size: 1753 1637 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5323 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2200 3983 1239 Size: 961 1783 2579 Fragments arranged by size: 2579 1783 961 Bpu10I CC'TnA_GC Cuts at: 2036 2036 Size: 5323 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3448 4313 4611 3448 Size: 865 298 4160 Fragments arranged by size: 4160 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3986 882 Size: 617 2487 2219 Fragments arranged by size: 2487 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3114 493 Size: 711 1910 2702 Fragments arranged by size: 2702 1910 711 BsaBI GATnn'nnATC Cuts at: 2363 5317 2363 Size: 2954 2369 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3130 3512 4147 275 Size: 53 83 186 936 1597 382 635 1451 Fragments arranged by size: 1597 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2436 3756 4154 4428 4575 4986 2436 Size: 1320 398 274 147 411 2773 Fragments arranged by size: 2773 1320 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2245 2275 1178 Size: 30 165 30 842 30 4226 Fragments arranged by size: 4226 842 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5323 BseYI C'CCAG_C Cuts at: 2028 4526 5022 2028 Size: 2498 496 2329 Fragments arranged by size: 2498 2329 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2078 1163 Size: 573 342 4408 Fragments arranged by size: 4408 573 342 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2773 3534 4562 1540 Size: 275 186 772 761 1028 2301 Fragments arranged by size: 2301 1028 772 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3384 3469 4540 3384 Size: 85 1071 4167 Fragments arranged by size: 4167 1071 85 BsmI GAATG_Cn' Cuts at: 2834 2927 2834 Size: 93 5230 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2168 2346 2642 3384 3469 4540 Size: 399 291 87 178 296 742 85 1071 Cuts at: 4540 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 296 291 178 87 85 BspEI T'CCGG_A Cuts at: 2436 4986 2436 Size: 2550 2773 BspHI T'CATG_A Cuts at: 3212 4942 3212 Size: 1730 3593 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2678 844 Size: 1834 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3210 1394 Size: 429 1387 3507 Fragments arranged by size: 3507 1387 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3818 3992 65 Size: 1183 2570 174 1396 Fragments arranged by size: 2570 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3967 4154 1700 Size: 110 2157 187 2869 Fragments arranged by size: 2869 2157 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5323 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5323 BssSI C'ACGA_G Cuts at: 3383 4395 3383 Size: 1012 4311 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5323 BstBI TT'CG_AA Cuts at: 4127 4127 Size: 5323 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5323 BstYI r'GATC_y Cuts at: 1972 2653 3020 3405 3422 4874 4960 4972 5318 Size: 681 367 385 17 1452 86 12 346 Cuts at: 5318 1972 Size: 1977 Fragments arranged by size: 1977 1452 681 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3832 Fragments arranged by size: 3832 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4584 Fragments arranged by size: 4584 683 56 BtsI GCAGTG_nn' Cuts at: 2839 3633 3653 2839 Size: 794 20 4509 Fragments arranged by size: 4509 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5323 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5288 DraI TTT'AAA Cuts at: 2627 2627 Size: 5323 DraIII CAC_nnn'GTG Cuts at: 2019 2019 Size: 5323 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4330 809 Size: 507 3014 1802 Fragments arranged by size: 3014 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2770 3659 Size: 54 1425 50 275 18 180 760 889 Cuts at: 3659 5288 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 760 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2770 1537 Size: 275 958 4090 Fragments arranged by size: 4090 958 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3253 1353 Size: 1900 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3910 4284 4430 387 Size: 1064 138 2321 374 146 1280 Fragments arranged by size: 2321 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2200 3386 3983 4770 5038 1239 Size: 393 568 1186 597 787 268 1524 Fragments arranged by size: 1524 1186 787 597 568 393 268 EcoO109I rG'GnC_Cy Cuts at: 2032 2185 2032 Size: 153 5170 EcoRI G'AATT_C Cuts at: 2290 2580 2632 3010 2290 Size: 290 52 378 4603 Fragments arranged by size: 4603 378 290 52 FspI TGC'GCA Cuts at: 3829 3829 Size: 5323 HaeII r_GCGC'y Cuts at: 1678 2093 4470 1678 Size: 415 2377 2531 Fragments arranged by size: 2531 2377 415 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2034 2066 2245 2277 2388 Size: 32 135 32 462 32 179 32 111 Cuts at: 2388 2420 2460 2492 3964 3996 4038 4070 1373 Size: 32 40 32 1472 32 42 32 2626 Fragments arranged by size: 2626 1472 462 179 135 111 42 40 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2614 2667 2913 669 Size: 988 281 676 53 246 3079 Fragments arranged by size: 3079 988 676 281 246 53 HindIII A'AGCT_T Cuts at: 748 748 Size: 5323 HpaI GTT'AAC Cuts at: 2913 2913 Size: 5323 KpnI G_GTAC'C Cuts at: 2648 2648 Size: 5323 MfeI C'AATT_G Cuts at: 2922 2922 Size: 5323 MluI A'CGCG_T Cuts at: 5199 5199 Size: 5323 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2231 4437 4621 1305 Size: 926 2206 184 2007 Fragments arranged by size: 2206 2007 926 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3501 Fragments arranged by size: 3501 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3281 3640 3799 518 Size: 648 2115 359 159 2042 Fragments arranged by size: 2115 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2739 3416 4564 4809 1606 Size: 1133 677 1148 245 2120 Fragments arranged by size: 2120 1148 1133 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3832 Fragments arranged by size: 3832 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5323 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5323 NotI GC'GGCC_GC Cuts at: 2770 2770 Size: 5323 NspI r_CATG'y Cuts at: 1132 1229 2681 4226 1132 Size: 97 1452 1545 2229 Fragments arranged by size: 2229 1545 1452 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5323 PciI A'CATG_T Cuts at: 4222 4222 Size: 5323 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5323 PmeI GTTT'AAAC Cuts at: 2627 2627 Size: 5323 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3392 3424 4905 4937 3392 Size: 32 1481 32 3778 Fragments arranged by size: 3778 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2185 2185 Size: 5323 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5323 PsiI TTA'TAA Cuts at: 2893 2893 Size: 5323 PstI C_TGCA'G Cuts at: 830 2460 2675 830 Size: 1630 215 3478 Fragments arranged by size: 3478 1630 215 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5323 SalI G'TCGA_C Cuts at: 1936 2612 2665 1936 Size: 676 53 4594 Fragments arranged by size: 4594 676 53 SanDI GG'GwC_CC Cuts at: 2185 2185 Size: 5323 SbfI CC_TGCA'GG Cuts at: 2675 2675 Size: 5323 ScaI AGT'ACT Cuts at: 3571 5283 3571 Size: 1712 3611 SexAI A'CCwGG_T Cuts at: 2015 2015 Size: 5323 SfcI C'TryA_G Cuts at: 826 1045 2131 2456 2671 3806 4487 4678 826 Size: 219 1086 325 215 1135 681 191 1471 Fragments arranged by size: 1471 1135 1086 681 325 219 215 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5323 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5323 SmaI CCC'GGG Cuts at: 1872 2650 1872 Size: 778 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3427 4328 4590 820 Size: 197 926 1484 901 262 1553 Fragments arranged by size: 1553 1484 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3114 493 Size: 2621 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5323 SphI G_CATG'C Cuts at: 1132 2681 1132 Size: 1549 3774 SspI AAT'ATT Cuts at: 5 52 3247 5 Size: 47 3195 2081 Fragments arranged by size: 3195 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5323 StyI C'CwwG_G Cuts at: 513 1065 2004 2295 2762 513 Size: 552 939 291 467 3074 Fragments arranged by size: 3074 939 552 467 291 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3350 3367 3520 3705 4150 325 Size: 1255 1770 17 153 185 445 1498 Fragments arranged by size: 1770 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3569 5281 96 Size: 274 80 33 51 1443 1592 1712 138 Fragments arranged by size: 1712 1592 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5283 5315 5283 Size: 32 5291 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5323 XbaI T'CTAG_A Cuts at: 2224 2659 2224 Size: 435 4888 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5323 XmaI C'CCGG_G Cuts at: 1870 2648 1870 Size: 778 4545 XmnI GAAnn'nnTTC Cuts at: 2584 3452 2584 Size: 868 4455 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3131 276 Size: 53 83 186 936 1597 2468 Fragments arranged by size: 2468 1597 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI