Restriction map for pFN21AE5219
 (Circular) (Six-base) MAPSORT of: pFN21AE5219.seq  Check: 6408  from: 1  to: 5323



pFN21AE5219  5323 bp

 With 182 enzymes: SgfI * 

                        January 30, 2014 17:15  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3133     278 
   Size:         53      83     186     936    1597    2468

  Fragments arranged by size: 

                2468    1597     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2613    2666    1937 
   Size:        676      53    4594

  Fragments arranged by size: 

                4594     676      53
 
Acc65I G'GTAC_C

Cuts at:   2644    2644 
   Size:       5323
 
AclI AA'CG_TT

Cuts at:   1563    3450    3823    1563 
   Size:       1887     373    3063

  Fragments arranged by size: 

                3063    1887     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3386    4770    5038    1632 
   Size:       1754    1384     268    1917

  Fragments arranged by size: 

                1917    1754    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5126
 
AflIII A'CryG_T

Cuts at:   1203    4222    5199    1203 
   Size:       3019     977    1327

  Fragments arranged by size: 

                3019    1327     977
 
AgeI A'CCGG_T

Cuts at:   4154    4154 
   Size:       5323
 
AhdI GACnn_n'nnGTC

Cuts at:   4052    4052 
   Size:       5323
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5323
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5323
 
AlwNI CAG_nnn'CTG

Cuts at:   3066    4638    3066 
   Size:       1572    3751
 
ApaLI G'TGCA_C

Cuts at:   3380    4536    3380 
   Size:       1156    4167
 
ApoI r'AATT_y

Cuts at:   1493    2290    2463    2580    2632    2858    3010    5119    1493 
   Size:        797     173     117      52     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     797     226     173     152     117      52
            
 
AseI AT'TA_AT

Cuts at:    160    1032    3877     160 
   Size:        872    2845    1606

  Fragments arranged by size: 

                2845    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5323
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2648    1870 
   Size:         73     705    4545

  Fragments arranged by size: 

                4545     705      73
 
BamHI G'GATC_C

Cuts at:   2653    3020    2653 
   Size:        367    4956
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2019    2076    2644    4099     618 
   Size:        325     217     627     232      57     568    1455    1842

  Fragments arranged by size: 

                1842    1455     627     568     325     232     217      57
            
 
BanII G_rGCy'C

Cuts at:   1391    2168    2346    2642    1391 
   Size:        777     178     296    4072

  Fragments arranged by size: 

                4072     777     296     178
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4352
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2447    2481    2593    2627    3514    3548    1903 
   Size:         34     510      34     112      34     887      34    3678

  Fragments arranged by size: 

                3678     887     510     112      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   3215    4425    3215 
   Size:       1210    4113
 
BclI T'GATC_A

Cuts at:   1548    2475    1548 
   Size:        927    4396
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    2350    3934 
   Size:        107     122      71     631     605     337     341    1584

Cuts at:   3934     136 
   Size:       1525

  Fragments arranged by size: 

                1584    1525     631     605     341     337     122     107
                  71
 
BglII A'GATC_T

Cuts at:   5318    5318 
   Size:       5323
 
BlpI GC'TnA_GC

Cuts at:   2740    2740 
   Size:       5323
 
Bme1580I G_kGCm'C

Cuts at:   1790    2081    3384    4540    1790 
   Size:        291    1303    1156    2573

  Fragments arranged by size: 

                2573    1303    1156     291
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5323
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3054    4012     442 
   Size:        416      53      83     399      24    1637     958    1753

  Fragments arranged by size: 

                1753    1637     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5323
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2200    3983    1239 
   Size:        961    1783    2579

  Fragments arranged by size: 

                2579    1783     961
 
Bpu10I CC'TnA_GC

Cuts at:   2036    2036 
   Size:       5323
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3448    4313    4611    3448 
   Size:        865     298    4160

  Fragments arranged by size: 

                4160     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3986     882 
   Size:        617    2487    2219

  Fragments arranged by size: 

                2487    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3114     493 
   Size:        711    1910    2702

  Fragments arranged by size: 

                2702    1910     711
 
BsaBI GATnn'nnATC

Cuts at:   2363    5317    2363 
   Size:       2954    2369
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3130    3512    4147     275 
   Size:         53      83     186     936    1597     382     635    1451

  Fragments arranged by size: 

                1597    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2436    3756    4154    4428    4575    4986    2436 
   Size:       1320     398     274     147     411    2773

  Fragments arranged by size: 

                2773    1320     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2245    2275    1178 
   Size:         30     165      30     842      30    4226

  Fragments arranged by size: 

                4226     842     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5323
 
BseYI C'CCAG_C

Cuts at:   2028    4526    5022    2028 
   Size:       2498     496    2329

  Fragments arranged by size: 

                2498    2329     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2078    1163 
   Size:        573     342    4408

  Fragments arranged by size: 

                4408     573     342
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2773    3534    4562    1540 
   Size:        275     186     772     761    1028    2301

  Fragments arranged by size: 

                2301    1028     772     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3384    3469    4540    3384 
   Size:         85    1071    4167

  Fragments arranged by size: 

                4167    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2834    2927    2834 
   Size:         93    5230
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2081    2168    2346    2642    3384    3469    4540 
   Size:        399     291      87     178     296     742      85    1071

Cuts at:   4540    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     399     296     291     178      87
                  85
 
BspEI T'CCGG_A

Cuts at:   2436    4986    2436 
   Size:       2550    2773
 
BspHI T'CATG_A

Cuts at:   3212    4942    3212 
   Size:       1730    3593
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2678     844 
   Size:       1834    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3210    1394 
   Size:        429    1387    3507

  Fragments arranged by size: 

                3507    1387     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3818    3992      65 
   Size:       1183    2570     174    1396

  Fragments arranged by size: 

                2570    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3967    4154    1700 
   Size:        110    2157     187    2869

  Fragments arranged by size: 

                2869    2157     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5323
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5323
 
BssSI C'ACGA_G

Cuts at:   3383    4395    3383 
   Size:       1012    4311
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5323
 
BstBI TT'CG_AA

Cuts at:   4127    4127 
   Size:       5323
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5323
 
BstYI r'GATC_y

Cuts at:   1972    2653    3020    3405    3422    4874    4960    4972    5318 
   Size:        681     367     385      17    1452      86      12     346

Cuts at:   5318    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     681     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3832

  Fragments arranged by size: 

                3832     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4584

  Fragments arranged by size: 

                4584     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2839    3633    3653    2839 
   Size:        794      20    4509

  Fragments arranged by size: 

                4509     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5323
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5288
 
DraI TTT'AAA

Cuts at:   2627    2627 
   Size:       5323
 
DraIII CAC_nnn'GTG

Cuts at:   2019    2019 
   Size:       5323
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4330     809 
   Size:        507    3014    1802

  Fragments arranged by size: 

                3014    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2010    2770    3659 
   Size:         54    1425      50     275      18     180     760     889

Cuts at:   3659    5288       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     760     275     180      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2770    1537 
   Size:        275     958    4090

  Fragments arranged by size: 

                4090     958     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3253    1353 
   Size:       1900    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3910    4284    4430     387 
   Size:       1064     138    2321     374     146    1280

  Fragments arranged by size: 

                2321    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2200    3386    3983    4770    5038    1239 
   Size:        393     568    1186     597     787     268    1524

  Fragments arranged by size: 

                1524    1186     787     597     568     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2032    2185    2032 
   Size:        153    5170
 
EcoRI G'AATT_C

Cuts at:   2290    2580    2632    3010    2290 
   Size:        290      52     378    4603

  Fragments arranged by size: 

                4603     378     290      52
 
FspI TGC'GCA

Cuts at:   3829    3829 
   Size:       5323
 
HaeII r_GCGC'y

Cuts at:   1678    2093    4470    1678 
   Size:        415    2377    2531

  Fragments arranged by size: 

                2531    2377     415
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2034    2066    2245    2277    2388 
   Size:         32     135      32     462      32     179      32     111

Cuts at:   2388    2420    2460    2492    3964    3996    4038    4070    1373 
   Size:         32      40      32    1472      32      42      32    2626

  Fragments arranged by size: 

                2626    1472     462     179     135     111      42      40
                  32      32      32      32      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2614    2667    2913     669 
   Size:        988     281     676      53     246    3079

  Fragments arranged by size: 

                3079     988     676     281     246      53
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5323
 
HpaI GTT'AAC

Cuts at:   2913    2913 
   Size:       5323
 
KpnI G_GTAC'C

Cuts at:   2648    2648 
   Size:       5323
 
MfeI C'AATT_G

Cuts at:   2922    2922 
   Size:       5323
 
MluI A'CGCG_T

Cuts at:   5199    5199 
   Size:       5323
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2231    4437    4621    1305 
   Size:        926    2206     184    2007

  Fragments arranged by size: 

                2206    2007     926     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3501

  Fragments arranged by size: 

                3501    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3281    3640    3799     518 
   Size:        648    2115     359     159    2042

  Fragments arranged by size: 

                2115    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2739    3416    4564    4809    1606 
   Size:       1133     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1133     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3832

  Fragments arranged by size: 

                3832     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5323
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5323
 
NotI GC'GGCC_GC

Cuts at:   2770    2770 
   Size:       5323
 
NspI r_CATG'y

Cuts at:   1132    1229    2681    4226    1132 
   Size:         97    1452    1545    2229

  Fragments arranged by size: 

                2229    1545    1452      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5323
 
PciI A'CATG_T

Cuts at:   4222    4222 
   Size:       5323
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5323
 
PmeI GTTT'AAAC

Cuts at:   2627    2627 
   Size:       5323
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3392    3424    4905    4937    3392 
   Size:         32    1481      32    3778

  Fragments arranged by size: 

                3778    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2185    2185 
   Size:       5323
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5323
 
PsiI TTA'TAA

Cuts at:   2893    2893 
   Size:       5323
 
PstI C_TGCA'G

Cuts at:    830    2460    2675     830 
   Size:       1630     215    3478

  Fragments arranged by size: 

                3478    1630     215
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5323
 
SalI G'TCGA_C

Cuts at:   1936    2612    2665    1936 
   Size:        676      53    4594

  Fragments arranged by size: 

                4594     676      53
 
SanDI GG'GwC_CC

Cuts at:   2185    2185 
   Size:       5323
 
SbfI CC_TGCA'GG

Cuts at:   2675    2675 
   Size:       5323
 
ScaI AGT'ACT

Cuts at:   3571    5283    3571 
   Size:       1712    3611
 
SexAI A'CCwGG_T

Cuts at:   2015    2015 
   Size:       5323
 
SfcI C'TryA_G

Cuts at:    826    1045    2131    2456    2671    3806    4487    4678     826 
   Size:        219    1086     325     215    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1086     681     325     219     215     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5323
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5323
 
SmaI CCC'GGG

Cuts at:   1872    2650    1872 
   Size:        778    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3427    4328    4590     820 
   Size:        197     926    1484     901     262    1553

  Fragments arranged by size: 

                1553    1484     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3114     493 
   Size:       2621    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5323
 
SphI G_CATG'C

Cuts at:   1132    2681    1132 
   Size:       1549    3774
 
SspI AAT'ATT

Cuts at:      5      52    3247       5 
   Size:         47    3195    2081

  Fragments arranged by size: 

                3195    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5323
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2295    2762     513 
   Size:        552     939     291     467    3074

  Fragments arranged by size: 

                3074     939     552     467     291
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3350    3367    3520    3705    4150     325 
   Size:       1255    1770      17     153     185     445    1498

  Fragments arranged by size: 

                1770    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3569    5281      96 
   Size:        274      80      33      51    1443    1592    1712     138

  Fragments arranged by size: 

                1712    1592    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5283    5315    5283 
   Size:         32    5291
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5323
 
XbaI T'CTAG_A

Cuts at:   2224    2659    2224 
   Size:        435    4888
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5323
 
XmaI C'CCGG_G

Cuts at:   1870    2648    1870 
   Size:        778    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2584    3452    2584 
   Size:        868    4455
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3131     276 
   Size:         53      83     186     936    1597    2468

  Fragments arranged by size: 

                2468    1597     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
  EcoICRI    EcoNI    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI
    PspXI     PsrI    PvuII    RsrII     SacI    SacII     SapI     SfiI
     SfoI     SrfI     SwaI     XcmI
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