(Linear) (Six-base) MAPSORT of: KIEE5219.seq Check: 3602 from: 1 to: 618 KIEE5219.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. AccI GT'mk_AC Cuts at: 0 608 618 Size: 608 10 ApoI r'AATT_y Cuts at: 0 285 458 575 618 Size: 285 173 117 43 Fragments arranged by size: 285 173 117 43 BanI G'GyrC_C Cuts at: 0 14 71 618 Size: 14 57 547 Fragments arranged by size: 547 57 14 BanII G_rGCy'C Cuts at: 0 163 341 618 Size: 163 178 277 Fragments arranged by size: 277 178 163 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 442 476 588 618 Size: 442 34 112 30 Fragments arranged by size: 442 112 34 30 BclI T'GATC_A Cuts at: 0 470 618 Size: 470 148 BglI GCCn_nnn'nGGC Cuts at: 0 345 618 Size: 345 273 Bme1580I G_kGCm'C Cuts at: 0 76 618 Size: 76 542 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 195 618 Size: 195 423 Bpu10I CC'TnA_GC Cuts at: 0 31 618 Size: 31 587 BsaBI GATnn'nnATC Cuts at: 0 358 618 Size: 358 260 BsaWI w'CCGG_w Cuts at: 0 431 618 Size: 431 187 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 240 270 618 Size: 240 30 348 Fragments arranged by size: 348 240 30 BseYI C'CCAG_C Cuts at: 0 23 618 Size: 23 595 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 618 Size: 73 545 Bsp1286I G_dGCh'C Cuts at: 0 76 163 341 618 Size: 76 87 178 277 Fragments arranged by size: 277 178 87 76 BspEI T'CCGG_A Cuts at: 0 431 618 Size: 431 187 DraIII CAC_nnn'GTG Cuts at: 0 14 618 Size: 14 604 EaeI y'GGCC_r Cuts at: 0 5 618 Size: 5 613 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 195 618 Size: 195 423 EcoO109I rG'GnC_Cy Cuts at: 0 27 180 618 Size: 27 153 438 Fragments arranged by size: 438 153 27 EcoRI G'AATT_C Cuts at: 0 285 575 618 Size: 285 290 43 Fragments arranged by size: 290 285 43 HaeII r_GCGC'y Cuts at: 0 88 618 Size: 88 530 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 29 61 240 272 383 415 455 487 Size: 29 32 179 32 111 32 40 32 Cuts at: 487 618 Size: 131 Fragments arranged by size: 179 131 111 40 32 32 32 32 29 HincII GTy'rAC Cuts at: 0 609 618 Size: 609 9 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 226 618 Size: 226 392 PpuMI rG'GwC_Cy Cuts at: 0 180 618 Size: 180 438 PstI C_TGCA'G Cuts at: 0 455 618 Size: 455 163 SalI G'TCGA_C Cuts at: 0 607 618 Size: 607 11 SanDI GG'GwC_CC Cuts at: 0 180 618 Size: 180 438 SexAI A'CCwGG_T Cuts at: 0 10 618 Size: 10 608 SfcI C'TryA_G Cuts at: 0 126 451 618 Size: 126 325 167 Fragments arranged by size: 325 167 126 StyI C'CwwG_G Cuts at: 0 290 618 Size: 290 328 XbaI T'CTAG_A Cuts at: 0 219 618 Size: 219 399 XmnI GAAnn'nnTTC Cuts at: 0 579 618 Size: 579 39 Enzymes that do cut: AccI ApoI BanI BanII BcgI BclI BglI Bme1580I BpmI Bpu10I BsaBI BsaWI BsaXI BseYI BsgI Bsp1286I BspEI DraIII EaeI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII MmeI PpuMI PstI SalI SanDI SexAI SfcI StyI XbaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaHI BseRI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XcmI XhoI XmaI ZraI