Restriction map for
pFN21AB6685
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
Acc65I AccIII AclI AfaI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmFI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CfoI ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KpnI
KspI MamI MfeI MroI MseI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NspI NspV PacI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsaI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 610 GACGTC GACGT^C
AccI 1264 GTCGAC GT^[AC][GT]AC
AcyI 610 GACGTC G[GA]^CG[CT]C
AflIII 411 ACGCGT A^C[GA][CT]GT
AlwNI 112 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 550 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 57 TGGCCA TGG^CCA
BcgI 1256 GCAAGGTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 940 CTAG C^TAG
BlpI 237 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 237 GCTGAGC GC^T[ACGT]AGC
BsaHI 610 GACGTC G[GA]^CG[CT]C
BsaI 205 GAGACC GGTCTC(1/5)
BseRI 22 CTCCTC GAGGAG(10/8)
BsmBI 815 GAGACG CGTCTC(1/5)
BspHI 768 TCATGA T^CATGA
BspMI 17 ACCTGC ACCTGC(4/8)
BsrFI 1199 ACCGGC [GA]^CCGG[CT]
BstAPI 366 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 237 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1199 ACCGGC [GA]^CCGG[CT]
EcoNI 841 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1 ATGCAT ATGCA^T
FauI 234 CCCGC CCCGC(4/6)
HgaI 817 GACGC GACGC(5/10)
Hin1I 610 GACGTC G[GA]^CG[CT]C
HincII 1264 GTCGAC GT[CT]^[GA]AC
HindII 1264 GTCGAC GT[CT]^[GA]AC
MaeI 940 CTAG C^TAG
MaeIII 95 GTAAC ^GT[ACGT]AC
MluI 411 ACGCGT A^CGCGT
MluNI 57 TGGCCA TGG^CCA
MscI 57 TGGCCA TGG^CCA
MslI 76 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 1148 TCGCGA TCG^CGA
NsiI 1 ATGCAT ATGCA^T
PaeR7I 569 CTCGAG C^TCGAG
PflFI 550 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 396 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 61 CAGCTG CAG^CTG
RcaI 768 TCATGA T^CATGA
SacI 598 GAGCTC GAGCT^C
SalI 1264 GTCGAC G^TCGAC
SmaI 210 CCCGGG CCC^GGG
Tth111I 550 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 396 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 569 CTCGAG C^TCGAG
XmaCI 210 CCCGGG C^CCGGG
XmaI 210 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 412 CGCG CG^CG
1149 CGCG
AlwI 300 GATCC GGATC(4/5)
1017 GGATC
Asp700I 660 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
868 GAAGAGGTTC
AspHI 422 GTGCTC G[AT]GC[AT]^C
598 GAGCTC
AvaI 210 CCCGGG C^[CT]CG[GA]G
569 CTCGAG
AvaII 680 GGACC G^G[AT]CC
744 GGACC
BanI 726 GGTGCC G^G[CT][GA]CC
1120 GGCACC
BanII 132 GGGCTC G[GA]GC[CT]^C
598 GAGCTC
BsgI 64 CTGCAC GTGCAG(16/14)
373 CTGCAC
BsiHKAI 422 GTGCTC G[AT]GC[AT]^C
598 GAGCTC
BsoBI 210 CCCGGG C^[CT]CG[GA]G
569 CTCGAG
BstUI 412 CGCG CG^CG
1149 CGCG
BstYI 299 AGATCC [GA]^GATC[CT]
1017 GGATCT
DraII 274 GGGGCCT [GA]G^G[ACGT]CC[CT]
308 AGGGCCT
DsaI 396 CCATGG C^C[GA][CT]GG
476 CCATGG
EcoO109I 274 GGGGCCT [GA]G^G[ACGT]CC[CT]
308 AGGGCCT
MflI 299 AGATCC [GA]^GATC[CT]
1017 GGATCT
MspA1I 61 CAGCTG C[AC]G^C[GT]G
235 CCGCTG
MvnI 412 CGCG CG^CG
1149 CGCG
NcoI 396 CCATGG C^CATGG
476 CCATGG
PleI 879 GACTC GAGTC(4/5)
340 GAGTC
PstI 739 CTGCAG CTGCA^G
1130 CTGCAG
SfcI 739 CTGCAG C^T[GA][CT]AG
1130 CTGCAG
TfiI 114 GATTC G^A[AT]TC
1193 GATTC
XcmI 260 CCATGCTTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1097 CCACCTTGGACGTGG
XhoII 299 AGATCC [GA]^GATC[CT]
1017 GGATCT
XmnI 660 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
868 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TseI Tsp509I TspRI
TthHB8I