(Circular) (Six-base) MAPSORT of: pFN21AB6685.seq Check: 640 from: 1 to: 5986 pFN21AB6685 5986 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2619 3796 278 Size: 53 83 186 936 1083 1177 2468 Fragments arranged by size: 2468 1177 1083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3270 3329 1937 Size: 1333 59 4594 Fragments arranged by size: 4594 1333 59 Acc65I G'GTAC_C Cuts at: 3307 3307 Size: 5986 AclI AA'CG_TT Cuts at: 1563 4113 4486 1563 Size: 2550 373 3063 Fragments arranged by size: 3063 2550 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2471 4049 5433 5701 1632 Size: 839 1578 1384 268 1917 Fragments arranged by size: 1917 1578 1384 839 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5789 AflIII A'CryG_T Cuts at: 1203 2416 4885 5862 1203 Size: 1213 2469 977 1327 Fragments arranged by size: 2469 1327 1213 977 AgeI A'CCGG_T Cuts at: 4817 4817 Size: 5986 AhdI GACnn_n'nnGTC Cuts at: 4715 4715 Size: 5986 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5986 AlwNI CAG_nnn'CTG Cuts at: 2122 3729 5301 2122 Size: 1607 1572 2807 Fragments arranged by size: 2807 1607 1572 ApaLI G'TGCA_C Cuts at: 4043 5199 4043 Size: 1156 4830 ApoI r'AATT_y Cuts at: 1493 2465 2929 3161 3295 3521 3673 5782 1493 Size: 972 464 232 134 226 152 2109 1697 Fragments arranged by size: 2109 1697 972 464 232 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 4540 160 Size: 872 3508 1606 Fragments arranged by size: 3508 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 AvaI C'yCGr_G Cuts at: 1870 1943 2215 2574 3311 1870 Size: 73 272 359 737 4545 Fragments arranged by size: 4545 737 359 272 73 BamHI G'GATC_C Cuts at: 3316 3683 3316 Size: 367 5619 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2731 3125 3307 4762 618 Size: 325 217 627 944 394 182 1455 1842 Fragments arranged by size: 1842 1455 944 627 394 325 217 182 BanII G_rGCy'C Cuts at: 1391 2141 2607 3305 1391 Size: 750 466 698 4072 Fragments arranged by size: 4072 750 698 466 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5015 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3250 3284 4177 4211 1903 Size: 34 50 34 1229 34 893 34 3678 Fragments arranged by size: 3678 1229 893 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3878 5088 3878 Size: 1210 4776 BclI T'GATC_A Cuts at: 1548 1548 Size: 5986 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 5986 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4597 136 Size: 107 122 71 631 605 2925 1525 Fragments arranged by size: 2925 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5981 5981 Size: 5986 BlpI GC'TnA_GC Cuts at: 2243 3403 2243 Size: 1160 4826 Bme1580I G_kGCm'C Cuts at: 1790 2537 2736 4047 5203 1790 Size: 747 199 1311 1156 2573 Fragments arranged by size: 2573 1311 1156 747 199 BmgBI CAC'GTC Cuts at: 1318 2560 3112 1318 Size: 1242 552 4192 Fragments arranged by size: 4192 1242 552 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3717 4675 442 Size: 416 53 83 399 24 2300 958 1753 Fragments arranged by size: 2300 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5986 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2871 3095 4646 1239 Size: 905 727 224 1551 2579 Fragments arranged by size: 2579 1551 905 727 224 Bpu10I CC'TnA_GC Cuts at: 2438 2438 Size: 5986 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2135 2330 2591 2665 4111 4976 5274 2135 Size: 195 261 74 1446 865 298 2847 Fragments arranged by size: 2847 1446 865 298 261 195 74 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2204 4649 882 Size: 617 705 2445 2219 Fragments arranged by size: 2445 2219 705 617 BsaAI yAC'GTr Cuts at: 493 1204 3777 493 Size: 711 2573 2702 Fragments arranged by size: 2702 2573 711 BsaBI GATnn'nnATC Cuts at: 5980 5980 Size: 5986 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2616 3793 4175 4810 Size: 53 83 186 936 1083 1177 382 635 Cuts at: 4810 275 Size: 1451 Fragments arranged by size: 1451 1177 1083 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4419 4817 5091 5238 5649 4419 Size: 398 274 147 411 4756 Fragments arranged by size: 4756 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5761 Fragments arranged by size: 5761 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2018 1186 Size: 832 5154 BseYI C'CCAG_C Cuts at: 2144 3010 5189 5685 2144 Size: 866 2179 496 2445 Fragments arranged by size: 2445 2179 866 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2054 2363 1163 Size: 573 318 309 4786 Fragments arranged by size: 4786 573 318 309 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3436 4197 5225 1540 Size: 275 186 1435 761 1028 2301 Fragments arranged by size: 2301 1435 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2431 2607 4047 4132 5203 2431 Size: 176 1440 85 1071 3214 Fragments arranged by size: 3214 1440 1071 176 85 BsmI GAATG_Cn' Cuts at: 3497 3590 3497 Size: 93 5893 BsmBI CGTCTCn'nnnn_ Cuts at: 2814 2814 Size: 5986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2431 2537 2607 2736 3305 4047 Size: 399 351 290 106 70 129 569 742 Cuts at: 4047 4132 5203 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 569 399 351 290 129 106 85 70 BspEI T'CCGG_A Cuts at: 5649 5649 Size: 5986 BspHI T'CATG_A Cuts at: 2773 3875 5605 2773 Size: 1102 1730 3154 Fragments arranged by size: 3154 1730 1102 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2031 3341 844 Size: 1187 1310 3489 Fragments arranged by size: 3489 1310 1187 BsrBI CCG'CTC Cuts at: 1394 1823 3873 1394 Size: 429 2050 3507 Fragments arranged by size: 3507 2050 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4481 4655 65 Size: 1183 3233 174 1396 Fragments arranged by size: 3233 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3204 4630 4817 1700 Size: 110 1394 1426 187 2869 Fragments arranged by size: 2869 1426 1394 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5986 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5986 BssSI C'ACGA_G Cuts at: 4046 5058 4046 Size: 1012 4974 BstAPI GCAn_nnn'nTGC Cuts at: 794 2377 794 Size: 1583 4403 BstBI TT'CG_AA Cuts at: 4790 4790 Size: 5986 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5986 BstYI r'GATC_y Cuts at: 1972 2304 3022 3316 3683 4068 4085 5537 5623 Size: 332 718 294 367 385 17 1452 86 Cuts at: 5623 5635 5981 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 718 385 367 346 332 294 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2401 2481 513 Size: 552 1336 80 4018 Fragments arranged by size: 4018 1336 552 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5247 Fragments arranged by size: 5247 683 56 BtsI GCAGTG_nn' Cuts at: 2431 3502 4296 4316 2431 Size: 1071 794 20 4101 Fragments arranged by size: 4101 1071 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5986 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5951 DraI TTT'AAA Cuts at: 3290 3290 Size: 5986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4993 809 Size: 507 3677 1802 Fragments arranged by size: 3677 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 2398 2484 Size: 54 1425 50 275 18 232 336 86 Cuts at: 2484 3433 4322 5951 8 Size: 949 889 1629 43 Fragments arranged by size: 1629 1425 949 889 336 275 232 86 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3433 1537 Size: 275 1621 4090 Fragments arranged by size: 4090 1621 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2510 2868 2918 3916 1353 Size: 1157 358 50 998 3423 Fragments arranged by size: 3423 1157 998 358 50 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4573 4947 5093 387 Size: 1064 138 2984 374 146 1280 Fragments arranged by size: 2984 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2605 2605 Size: 5986 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2471 2871 3095 4049 4646 5433 Size: 393 512 327 400 224 954 597 787 Cuts at: 5433 5701 1239 Size: 268 1524 Fragments arranged by size: 1524 954 787 597 512 400 393 327 268 224 EcoNI CCTnn'n_nnAGG Cuts at: 2850 2850 Size: 5986 EcoO109I rG'GnC_Cy Cuts at: 2280 2314 2280 Size: 34 5952 EcoRI G'AATT_C Cuts at: 3295 3673 3295 Size: 378 5608 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2245 2635 3121 2245 Size: 390 486 5110 Fragments arranged by size: 5110 486 390 FspI TGC'GCA Cuts at: 4492 4492 Size: 5986 HaeII r_GCGC'y Cuts at: 1678 5133 1678 Size: 3455 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3014 3046 4627 4659 4701 Size: 32 135 32 1442 32 1581 32 42 Cuts at: 4701 4733 1373 Size: 32 2626 Fragments arranged by size: 2626 1581 1442 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3271 3330 3576 669 Size: 988 281 1333 59 246 3079 Fragments arranged by size: 3079 1333 988 281 246 59 HindIII A'AGCT_T Cuts at: 748 748 Size: 5986 HpaI GTT'AAC Cuts at: 3576 3576 Size: 5986 KpnI G_GTAC'C Cuts at: 3311 3311 Size: 5986 MfeI C'AATT_G Cuts at: 3585 3585 Size: 5986 MluI A'CGCG_T Cuts at: 2416 5862 2416 Size: 3446 2540 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2020 5100 5284 1305 Size: 715 3080 184 2007 Fragments arranged by size: 3080 2007 715 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2064 10 Size: 54 1425 343 232 3932 Fragments arranged by size: 3932 1425 343 232 54 MslI CAynn'nnrTG Cuts at: 518 1166 2085 3944 4303 4462 518 Size: 648 919 1859 359 159 2042 Fragments arranged by size: 2042 1859 919 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2068 2242 3402 4079 5227 5472 1606 Size: 462 174 1160 677 1148 245 2120 Fragments arranged by size: 2120 1160 1148 677 462 245 174 NcoI C'CATG_G Cuts at: 513 1065 2401 2481 513 Size: 552 1336 80 4018 Fragments arranged by size: 4018 1336 552 80 NdeI CA'TA_TG Cuts at: 387 387 Size: 5986 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5986 NotI GC'GGCC_GC Cuts at: 3433 3433 Size: 5986 NruI TCG'CGA Cuts at: 3155 3155 Size: 5986 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 5986 NspI r_CATG'y Cuts at: 1132 1229 3344 4889 1132 Size: 97 2115 1545 2229 Fragments arranged by size: 2229 2115 1545 97 PasI CC'CwG_GG Cuts at: 1348 2275 1348 Size: 927 5059 PciI A'CATG_T Cuts at: 4885 4885 Size: 5986 PflMI CCAn_nnn'nTGG Cuts at: 1108 2407 1108 Size: 1299 4687 PmeI GTTT'AAAC Cuts at: 3290 3290 Size: 5986 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4055 4087 5568 5600 4055 Size: 32 1481 32 4441 Fragments arranged by size: 4441 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5986 PsiI TTA'TAA Cuts at: 3556 3556 Size: 5986 PspXI vC'TCGA_Gb Cuts at: 2574 2574 Size: 5986 PstI C_TGCA'G Cuts at: 830 2748 3139 3338 830 Size: 1918 391 199 3478 Fragments arranged by size: 3478 1918 391 199 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5986 PvuII CAG'CTG Cuts at: 2068 2068 Size: 5986 SacI G_AGCT'C Cuts at: 2607 2607 Size: 5986 SalI G'TCGA_C Cuts at: 1936 3269 3328 1936 Size: 1333 59 4594 Fragments arranged by size: 4594 1333 59 SbfI CC_TGCA'GG Cuts at: 3338 3338 Size: 5986 ScaI AGT'ACT Cuts at: 4234 5946 4234 Size: 1712 4274 SfcI C'TryA_G Cuts at: 826 1045 2744 3135 3334 4469 5150 5341 826 Size: 219 1699 391 199 1135 681 191 1471 Fragments arranged by size: 1699 1471 1135 681 391 219 199 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5986 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5986 SmaI CCC'GGG Cuts at: 1872 2217 3313 1872 Size: 345 1096 4545 Fragments arranged by size: 4545 1096 345 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 2309 2574 2606 2680 4090 Size: 197 926 207 159 265 32 74 1410 Cuts at: 4090 4991 5253 820 Size: 901 262 1553 Fragments arranged by size: 1553 1410 926 901 265 262 207 197 159 74 32 SnaBI TAC'GTA Cuts at: 493 3777 493 Size: 3284 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5986 SphI G_CATG'C Cuts at: 1132 3344 1132 Size: 2212 3774 SspI AAT'ATT Cuts at: 5 52 3910 5 Size: 47 3858 2081 Fragments arranged by size: 3858 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5986 StyI C'CwwG_G Cuts at: 513 1065 2401 2481 2851 3105 3425 513 Size: 552 1336 80 370 254 320 3074 Fragments arranged by size: 3074 1336 552 370 320 254 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3254 4013 4030 4183 4368 4813 325 Size: 1255 1674 759 17 153 185 445 1498 Fragments arranged by size: 1674 1498 1255 759 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4232 5944 96 Size: 274 80 33 51 1443 2255 1712 138 Fragments arranged by size: 2255 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5946 5978 5946 Size: 32 5954 Tth111I GACn'n_nGTC Cuts at: 1316 2558 1316 Size: 1242 4744 XbaI T'CTAG_A Cuts at: 3322 3322 Size: 5986 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2272 3109 2272 Size: 837 5149 XhoI C'TCGA_G Cuts at: 1943 2574 1943 Size: 631 5355 XmaI C'CCGG_G Cuts at: 1870 2215 3311 1870 Size: 345 1096 4545 Fragments arranged by size: 4545 1096 345 XmnI GAAnn'nnTTC Cuts at: 2669 2877 4115 2669 Size: 208 1238 4540 Fragments arranged by size: 4540 1238 208 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2617 3794 276 Size: 53 83 186 936 1083 1177 2468 Fragments arranged by size: 2468 1177 1083 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI