################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbsI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FbaI FseI HindIII HpaI KpnI KspI MamI MfeI MluI MroI MslI MunI NaeI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 1218 GAATTC [GA]^AATT[CT] AcyI 800 GGCGCC G[GA]^CG[CT]C AfaI 657 GTAC GT^AC ApaI 843 GGGCCC GGGCC^C ApoI 1218 GAATTC [GA]^AATT[CT] AspHI 712 GAGCAC G[AT]GC[AT]^C AviII 584 TGCGCA TGC^GCA BalI 594 TGGCCA TGG^CCA BamHI 225 GGATCC G^GATCC BanI 800 GGCGCC G^G[CT][GA]CC BbeI 800 GGCGCC GGCGC^C BbrPI 751 CACGTG CAC^GTG BsaAI 751 CACGTG [CT]AC^GT[GA] BsaHI 800 GGCGCC G[GA]^CG[CT]C BsaI 986 GGTCTC GGTCTC(1/5) BsiHKAI 712 GAGCAC G[AT]GC[AT]^C BspMI 694 GCAGGT ACCTGC(4/8) BsrBI 565 CCGCTC CCGCTC(-3/-3) BstAPI 69 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EcoNI 49 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1218 GAATTC G^AATTC EcoRV 493 GATATC GAT^ATC FspI 584 TGCGCA TGC^GCA Hin1I 800 GGCGCC G[GA]^CG[CT]C HincII 784 GTTGAC GT[CT]^[GA]AC HindII 784 GTTGAC GT[CT]^[GA]AC KasI 800 GGCGCC G^GCGCC MaeII 752 ACGT A^CGT MluNI 594 TGGCCA TGG^CCA MscI 594 TGGCCA TGG^CCA NarI 800 GGCGCC GG^CGCC PflMI 17 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 751 CACGTG CAC^GTG PmlI 751 CACGTG CAC^GTG RsaI 657 GTAC GT^AC SapI 155 GCTCTTC GCTCTTC(1/4) SexAI 1095 ACCTGGT A^CC[AT]GGT SfiI 816 GGCCGTGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 800 GGCGCC GGC^GCC SmlI 365 CTCAAG C^T[CT][GA]AG Tsp509I 1219 AATT ^AATT Van91I 17 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccII 468 CGCG CG^CG 1287 CGCG AlwNI 18 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 871 CAGTTGCTG BbvCI 762 CCTCAGC CCTCAGC(-5/-2) 898 GCTGAGG BfaI 628 CTAG C^TAG 1246 CTAG BlpI 1057 GCTAAGC GC^T[ACGT]AGC 1201 GCTGAGC Bpu1102I 1057 GCTAAGC GC^T[ACGT]AGC 1201 GCTGAGC BstUI 468 CGCG CG^CG 1287 CGCG CelII 1057 GCTAAGC GC^T[ACGT]AGC 1201 GCTGAGC EcoT14I 143 CCTTGG C^C[AT][AT]GG 794 CCTTGG HaeII 422 GGCGCT [GA]GCGC^[CT] 800 GGCGCC HgaI 1131 GACGC GACGC(5/10) 1283 GCGTC MaeI 628 CTAG C^TAG 1246 CTAG MseI 293 TTAA T^TAA 1034 TTAA MvnI 468 CGCG CG^CG 1287 CGCG PstI 667 CTGCAG CTGCA^G 692 CTGCAG StyI 143 CCTTGG C^C[AT][AT]GG 794 CCTTGG TfiI 136 GATTC G^A[AT]TC 208 GATTC Tru9I 293 TTAA T^TAA 1034 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AluI AlwI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PleI PpuMI PshAI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI TseI Tsp45I TspRI XhoII XmaCI XmaI