Restriction map for
pFN21AE4442
(Circular) (Six-base) MAPSORT of: pFN21AE4442.seq Check: 6075 from: 1 to: 6082
pFN21AE4442 6082 bp
With 182 enzymes: SgfI *
March 28, 2012 14:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3892 278
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3425 1937
Size: 1488 4594
Acc65I G'GTAC_C
Cuts at: 3403 3403
Size: 6082
AclI AA'CG_TT
Cuts at: 1563 4209 4582 1563
Size: 2646 373 3063
Fragments arranged by size:
3063 2646 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2313 2348 2950 4145 5529 5797 1632
Size: 681 35 602 1195 1384 268 1917
Fragments arranged by size:
1917 1384 1195 681 602 268 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5885
AflIII A'CryG_T
Cuts at: 1203 4981 5958 1203
Size: 3778 977 1327
Fragments arranged by size:
3778 1327 977
AgeI A'CCGG_T
Cuts at: 4913 4913
Size: 6082
AhdI GACnn_n'nnGTC
Cuts at: 4811 4811
Size: 6082
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6082
AlwNI CAG_nnn'CTG
Cuts at: 2028 2881 3825 5397 2028
Size: 853 944 1572 2713
Fragments arranged by size:
2713 1572 944 853
ApaI G_GGCC'C
Cuts at: 2852 2852
Size: 6082
ApaLI G'TGCA_C
Cuts at: 4139 5295 4139
Size: 1156 4926
ApoI r'AATT_y
Cuts at: 1493 3223 3391 3617 3769 5878 1493
Size: 1730 168 226 152 2109 1697
Fragments arranged by size:
2109 1730 1697 226 168 152
AseI AT'TA_AT
Cuts at: 160 1032 4636 160
Size: 872 3604 1606
Fragments arranged by size:
3604 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
AvaI C'yCGr_G
Cuts at: 1870 1943 2454 2651 2809 3198 3407 1870
Size: 73 511 197 158 389 209 4545
Fragments arranged by size:
4545 511 389 209 197 158 73
BamHI G'GATC_C
Cuts at: 2230 3412 3779 2230
Size: 1182 367 4533
Fragments arranged by size:
4533 1182 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2805 3403 4858 618
Size: 325 217 627 1018 598 1455 1842
Fragments arranged by size:
1842 1455 1018 627 598 325 217
BanII G_rGCy'C
Cuts at: 1391 2324 2671 2714 2852 3401 1391
Size: 933 347 43 138 549 4072
Fragments arranged by size:
4072 933 549 347 138 43
BbeI G_GCGC'C
Cuts at: 2809 2809
Size: 6082
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5111
BbvCI CC'TCA_GC
Cuts at: 2768 2904 2768
Size: 136 5946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4273 4307 1903
Size: 34 2336 34 3678
Fragments arranged by size:
3678 2336 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3974 5184 3974
Size: 1210 4872
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6082
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2629 2814 2828
Size: 107 122 71 631 605 957 185 14
Cuts at: 2828 4693 136
Size: 1865 1525
Fragments arranged by size:
1865 1525 957 631 605 185 122 107
71 14
BglII A'GATC_T
Cuts at: 6077 6077
Size: 6082
BlpI GC'TnA_GC
Cuts at: 3063 3207 3499 3063
Size: 144 292 5646
Fragments arranged by size:
5646 292 144
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2852 3016 4143 5299 1790
Size: 231 831 164 1127 1156 2573
Fragments arranged by size:
2573 1156 1127 831 231 164
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6082
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2192 2196 2698
Size: 416 53 83 399 24 775 4 502
Cuts at: 2698 3813 4771 442
Size: 1115 958 1753
Fragments arranged by size:
1753 1115 958 775 502 416 399 83
53 24 4
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6082
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2929 2929
Size: 6082
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2043 2277 2295 2382 2861 3201 3396 4742
Size: 804 234 18 87 479 340 195 1346
Cuts at: 4742 1239
Size: 2579
Fragments arranged by size:
2579 1346 804 479 340 234 195 87
18
Bpu10I CC'TnA_GC
Cuts at: 2768 2904 2768
Size: 136 5946
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2355 4207 5072 5370 2355
Size: 1852 865 298 3067
Fragments arranged by size:
3067 1852 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2997 4745 882
Size: 617 1498 1748 2219
Fragments arranged by size:
2219 1748 1498 617
BsaAI yAC'GTr
Cuts at: 493 1204 2758 3873 493
Size: 711 1554 1115 2702
Fragments arranged by size:
2702 1554 1115 711
BsaBI GATnn'nnATC
Cuts at: 6076 6076
Size: 6082
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2806 3889 4271 4906
Size: 53 83 186 936 1273 1083 382 635
Cuts at: 4906 275
Size: 1451
Fragments arranged by size:
1451 1273 1083 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4515 4913 5187 5334 5745 4515
Size: 398 274 147 411 4852
Fragments arranged by size:
4852 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2608 2638 1178
Size: 30 165 30 1205 30 4622
Fragments arranged by size:
4622 1205 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2169 2204 2240 2258 2276 2294 2858 1186
Size: 983 35 36 18 18 18 564 4410
Fragments arranged by size:
4410 983 564 36 35 18 18 18
BseYI C'CCAG_C
Cuts at: 2021 2464 2508 3355 5285 5781 2021
Size: 443 44 847 1930 496 2322
Fragments arranged by size:
2322 1930 847 496 443 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2669 2931 3323 1163
Size: 573 933 262 392 3922
Fragments arranged by size:
3922 933 573 392 262
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3532 4293 5321 1540
Size: 275 186 1531 761 1028 2301
Fragments arranged by size:
2301 1531 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2721 4143 4228 5299 2721
Size: 1422 85 1071 3504
Fragments arranged by size:
3504 1422 1071 85
BsmI GAATG_Cn'
Cuts at: 3593 3686 3593
Size: 93 5989
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2324 2671 2714 2721 2852 3016
Size: 399 231 303 347 43 7 131 164
Cuts at: 3016 3401 4143 4228 5299 1391
Size: 385 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 385 347 303 231
164 131 85 43 7
BspEI T'CCGG_A
Cuts at: 5745 5745
Size: 6082
BspHI T'CATG_A
Cuts at: 3971 5701 3971
Size: 1730 4352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2690 3437 844
Size: 1846 747 3489
Fragments arranged by size:
3489 1846 747
BsrBI CCG'CTC
Cuts at: 1394 1823 2572 3969 1394
Size: 429 749 1397 3507
Fragments arranged by size:
3507 1397 749 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4577 4751 65
Size: 1183 3329 174 1396
Fragments arranged by size:
3329 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4726 4913 1700
Size: 110 2916 187 2869
Fragments arranged by size:
2916 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6082
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6082
BssSI C'ACGA_G
Cuts at: 4142 5154 4142
Size: 1012 5070
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2080 794
Size: 1286 4796
BstBI TT'CG_AA
Cuts at: 4886 4886
Size: 6082
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6082
BstYI r'GATC_y
Cuts at: 1972 2194 2230 2248 2266 2284 2338 2365 2751
Size: 222 36 18 18 18 54 27 386
Cuts at: 2751 3412 3779 4164 4181 5633 5719 5731 6077
Size: 661 367 385 17 1452 86 12 346
Cuts at: 6077 1972
Size: 1977
Fragments arranged by size:
1977 1452 661 386 385 367 346 222
86 54 36 27 18 18 18 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4591
Fragments arranged by size:
4591 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5343
Fragments arranged by size:
5343 683 56
BtsI GCAGTG_nn'
Cuts at: 2087 3598 4392 4412 2087
Size: 1511 794 20 3757
Fragments arranged by size:
3757 1511 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6082
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6047
DraI TTT'AAA
Cuts at: 3386 3386
Size: 6082
DraIII CAC_nnn'GTG
Cuts at: 2608 2725 3093 2608
Size: 117 368 5597
Fragments arranged by size:
5597 368 117
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5089 809
Size: 507 3773 1802
Fragments arranged by size:
3773 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2599 2820 2829
Size: 54 1425 50 275 18 769 221 9
Cuts at: 2829 3529 4418 6047 8
Size: 700 889 1629 43
Fragments arranged by size:
1629 1425 889 769 700 275 221 54
50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 3529 1537
Size: 275 1717 4090
Fragments arranged by size:
4090 1717 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2167 2202 2310 4012 1353
Size: 814 35 108 1702 3423
Fragments arranged by size:
3423 1702 814 108 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2492 4669 5043 5189 387
Size: 1064 138 903 2177 374 146 1280
Fragments arranged by size:
2177 1280 1064 903 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2277 2295 2313 2348 2382 2861
Size: 393 411 234 18 18 35 34 479
Cuts at: 2861 2950 3201 3396 4145 4742 5529 5797 1239
Size: 89 251 195 749 597 787 268 1524
Fragments arranged by size:
1524 787 749 597 479 411 393 268
251 234 195 89 35 34 18 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2058 2058
Size: 6082
EcoO109I rG'GnC_Cy
Cuts at: 2648 2848 2926 3098 2648
Size: 200 78 172 5632
Fragments arranged by size:
5632 200 172 78
EcoRI G'AATT_C
Cuts at: 3223 3391 3769 3223
Size: 168 378 5536
Fragments arranged by size:
5536 378 168
EcoRV GAT'ATC
Cuts at: 2500 2500
Size: 6082
FspI TGC'GCA
Cuts at: 2591 4588 2591
Size: 1997 4085
HaeII r_GCGC'y
Cuts at: 1678 2431 2809 5229 1678
Size: 753 378 2420 2531
Fragments arranged by size:
2531 2420 753 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2608 2640 4723 4755 4797
Size: 32 135 32 1036 32 2083 32 42
Cuts at: 4797 4829 1373
Size: 32 2626
Fragments arranged by size:
2626 2083 1036 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2791 3426 3672 669
Size: 988 281 853 635 246 3079
Fragments arranged by size:
3079 988 853 635 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6082
HpaI GTT'AAC
Cuts at: 3672 3672
Size: 6082
KasI G'GCGC_C
Cuts at: 2805 2805
Size: 6082
KpnI G_GTAC'C
Cuts at: 3407 3407
Size: 6082
MfeI C'AATT_G
Cuts at: 3681 3681
Size: 6082
MluI A'CGCG_T
Cuts at: 5958 5958
Size: 6082
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5196 5380 1305
Size: 3891 184 2007
Fragments arranged by size:
3891 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2601 10
Size: 54 1425 343 769 3491
Fragments arranged by size:
3491 1425 769 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4040 4399 4558 518
Size: 648 2874 359 159 2042
Fragments arranged by size:
2874 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2677 3121 3220 3498 4175 5323 5568 1606
Size: 1071 444 99 278 677 1148 245 2120
Fragments arranged by size:
2120 1148 1071 677 444 278 245 99
NarI GG'CG_CC
Cuts at: 2806 2806
Size: 6082
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4591
Fragments arranged by size:
4591 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6082
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6082
NotI GC'GGCC_GC
Cuts at: 3529 3529
Size: 6082
NspI r_CATG'y
Cuts at: 1132 1229 3440 4985 1132
Size: 97 2211 1545 2229
Fragments arranged by size:
2229 2211 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3016 1348
Size: 1668 4414
PciI A'CATG_T
Cuts at: 4981 4981
Size: 6082
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2028 1108
Size: 920 5162
PfoI T'CCnGG_A
Cuts at: 2359 2359
Size: 6082
PmeI GTTT'AAAC
Cuts at: 3386 3386
Size: 6082
PmlI CAC'GTG
Cuts at: 2758 2758
Size: 6082
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4151 4183 5664 5696 4151
Size: 32 1481 32 4537
Fragments arranged by size:
4537 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2648 2926 3098 2648
Size: 278 172 5632
Fragments arranged by size:
5632 278 172
PshAI GACnn'nnGTC
Cuts at: 1613 2418 2924 3192 1613
Size: 805 506 268 4503
Fragments arranged by size:
4503 805 506 268
PsiI TTA'TAA
Cuts at: 3652 3652
Size: 6082
PspOMI G'GGCC_C
Cuts at: 2848 2848
Size: 6082
PstI C_TGCA'G
Cuts at: 830 2676 2701 3434 830
Size: 1846 25 733 3478
Fragments arranged by size:
3478 1846 733 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6082
PvuII CAG'CTG
Cuts at: 2677 3121 3220 2677
Size: 444 99 5539
Fragments arranged by size:
5539 444 99
SalI G'TCGA_C
Cuts at: 1936 3424 1936
Size: 1488 4594
SanDI GG'GwC_CC
Cuts at: 2648 2648
Size: 6082
SapI GCTCTTCn'nnn_
Cuts at: 2167 2167
Size: 6082
SbfI CC_TGCA'GG
Cuts at: 3434 3434
Size: 6082
ScaI AGT'ACT
Cuts at: 4330 6042 4330
Size: 1712 4370
SexAI A'CCwGG_T
Cuts at: 3100 3100
Size: 6082
SfcI C'TryA_G
Cuts at: 826 1045 2672 2697 3116 3430 4565 5246 5437
Size: 219 1627 25 419 314 1135 681 191
Cuts at: 5437 826
Size: 1471
Fragments arranged by size:
1627 1471 1135 681 419 314 219 191
25
SfiI GGCCn_nnn'nGGCC
Cuts at: 2828 2828
Size: 6082
SfoI GGC'GCC
Cuts at: 2807 2807
Size: 6082
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6082
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6082
SmaI CCC'GGG
Cuts at: 1872 2456 2653 2811 3409 1872
Size: 584 197 158 598 4545
Fragments arranged by size:
4545 598 584 197 158
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2370 4186 5087 5349 820
Size: 197 926 427 1816 901 262 1553
Fragments arranged by size:
1816 1553 926 901 427 262 197
SnaBI TAC'GTA
Cuts at: 493 3873 493
Size: 3380 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6082
SphI G_CATG'C
Cuts at: 1132 3440 1132
Size: 2308 3774
SspI AAT'ATT
Cuts at: 5 52 4006 5
Size: 47 3954 2081
Fragments arranged by size:
3954 2081 47
StuI AGG'CCT
Cuts at: 1517 2064 2798 2817 3167 1517
Size: 547 734 19 350 4432
Fragments arranged by size:
4432 734 547 350 19
StyI C'CwwG_G
Cuts at: 513 1065 2004 2148 2799 3521 513
Size: 552 939 144 651 722 3074
Fragments arranged by size:
3074 939 722 651 552 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2233 4109 4126 4279 4464 4909 325
Size: 1255 653 1876 17 153 185 445 1498
Fragments arranged by size:
1876 1498 1255 653 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4328 6040 96
Size: 274 80 33 51 1443 2351 1712 138
Fragments arranged by size:
2351 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6042 6074 6042
Size: 32 6050
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6082
XbaI T'CTAG_A
Cuts at: 3418 3418
Size: 6082
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6082
XmaI C'CCGG_G
Cuts at: 1870 2454 2651 2809 3407 1870
Size: 584 197 158 598 4545
Fragments arranged by size:
4545 598 584 197 158
XmnI GAAnn'nnTTC
Cuts at: 4211 4211
Size: 6082
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3890 276
Size: 53 83 186 936 2356 2468
Fragments arranged by size:
2468 2356 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII
SacI SacII SrfI SwaI XcmI