(Linear) (Six-base) MAPSORT of: KIEE4442.seq Check: 5934 from: 1 to: 1377 KIEE4442.orf With 182 enzymes: SgfI * March 22, 2012 18:02 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 308 343 945 1377 Size: 308 35 602 432 Fragments arranged by size: 602 432 308 35 AlwNI CAG_nnn'CTG Cuts at: 0 23 876 1377 Size: 23 853 501 Fragments arranged by size: 853 501 23 ApaI G_GGCC'C Cuts at: 0 847 1377 Size: 847 530 ApoI r'AATT_y Cuts at: 0 1218 1377 Size: 1218 159 AvaI C'yCGr_G Cuts at: 0 449 646 804 1193 1377 Size: 449 197 158 389 184 Fragments arranged by size: 449 389 197 184 158 BamHI G'GATC_C Cuts at: 0 225 1377 Size: 225 1152 BanI G'GyrC_C Cuts at: 0 800 1377 Size: 800 577 BanII G_rGCy'C Cuts at: 0 319 666 709 847 1377 Size: 319 347 43 138 530 Fragments arranged by size: 530 347 319 138 43 BbeI G_GCGC'C Cuts at: 0 804 1377 Size: 804 573 BbvCI CC'TCA_GC Cuts at: 0 763 899 1377 Size: 763 136 478 Fragments arranged by size: 763 478 136 BglI GCCn_nnn'nGGC Cuts at: 0 624 809 823 1377 Size: 624 185 14 554 Fragments arranged by size: 624 554 185 14 BlpI GC'TnA_GC Cuts at: 0 1058 1202 1377 Size: 1058 144 175 Fragments arranged by size: 1058 175 144 Bme1580I G_kGCm'C Cuts at: 0 16 847 1011 1377 Size: 16 831 164 366 Fragments arranged by size: 831 366 164 16 BmrI ACTGGGnnnn_n' Cuts at: 0 187 191 693 1377 Size: 187 4 502 684 Fragments arranged by size: 684 502 187 4 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 924 1377 Size: 924 453 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 38 272 290 377 856 1196 1377 Size: 38 234 18 87 479 340 181 Fragments arranged by size: 479 340 234 181 87 38 18 Bpu10I CC'TnA_GC Cuts at: 0 763 899 1377 Size: 763 136 478 Fragments arranged by size: 763 478 136 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 350 1377 Size: 350 1027 BsaI GGTCTCn'nnnn_ Cuts at: 0 992 1377 Size: 992 385 BsaAI yAC'GTr Cuts at: 0 753 1377 Size: 753 624 BsaHI Gr'CG_yC Cuts at: 0 801 1377 Size: 801 576 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 603 633 1377 Size: 603 30 744 Fragments arranged by size: 744 603 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 164 199 235 253 271 289 853 1377 Size: 164 35 36 18 18 18 564 524 Fragments arranged by size: 564 524 164 36 35 18 18 18 BseYI C'CCAG_C Cuts at: 0 16 459 503 1350 1377 Size: 16 443 44 847 27 Fragments arranged by size: 847 443 44 27 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 664 926 1318 1377 Size: 664 262 392 59 Fragments arranged by size: 664 392 262 59 BsiHKAI G_wGCw'C Cuts at: 0 716 1377 Size: 716 661 Bsp1286I G_dGCh'C Cuts at: 0 16 319 666 709 716 847 1011 1377 Size: 16 303 347 43 7 131 164 366 Fragments arranged by size: 366 347 303 164 131 43 16 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 685 1377 Size: 685 692 BsrBI CCG'CTC Cuts at: 0 567 1377 Size: 567 810 BstAPI GCAn_nnn'nTGC Cuts at: 0 75 1377 Size: 75 1302 BstYI r'GATC_y Cuts at: 0 189 225 243 261 279 333 360 746 Size: 189 36 18 18 18 54 27 386 Cuts at: 746 1377 Size: 631 Fragments arranged by size: 631 386 189 54 36 27 18 18 18 BtsI GCAGTG_nn' Cuts at: 0 82 1377 Size: 82 1295 DraIII CAC_nnn'GTG Cuts at: 0 603 720 1088 1377 Size: 603 117 368 289 Fragments arranged by size: 603 368 289 117 EaeI y'GGCC_r Cuts at: 0 594 815 824 1377 Size: 594 221 9 553 Fragments arranged by size: 594 553 221 9 EarI CTCTTCn'nnn_ Cuts at: 0 162 197 305 1377 Size: 162 35 108 1072 Fragments arranged by size: 1072 162 108 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 487 1377 Size: 487 890 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 38 272 290 308 343 377 856 945 Size: 38 234 18 18 35 34 479 89 Cuts at: 945 1196 1377 Size: 251 181 Fragments arranged by size: 479 251 234 181 89 38 35 34 18 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 53 1377 Size: 53 1324 EcoO109I rG'GnC_Cy Cuts at: 0 643 843 921 1093 1377 Size: 643 200 78 172 284 Fragments arranged by size: 643 284 200 172 78 EcoRI G'AATT_C Cuts at: 0 1218 1377 Size: 1218 159 EcoRV GAT'ATC Cuts at: 0 495 1377 Size: 495 882 FspI TGC'GCA Cuts at: 0 586 1377 Size: 586 791 HaeII r_GCGC'y Cuts at: 0 426 804 1377 Size: 426 378 573 Fragments arranged by size: 573 426 378 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 603 635 1377 Size: 603 32 742 Fragments arranged by size: 742 603 32 HincII GTy'rAC Cuts at: 0 786 1377 Size: 786 591 KasI G'GCGC_C Cuts at: 0 800 1377 Size: 800 577 MscI TGG'CCA Cuts at: 0 596 1377 Size: 596 781 MspA1I CmG'CkG Cuts at: 0 672 1116 1215 1377 Size: 672 444 99 162 Fragments arranged by size: 672 444 162 99 NarI GG'CG_CC Cuts at: 0 801 1377 Size: 801 576 PasI CC'CwG_GG Cuts at: 0 1011 1377 Size: 1011 366 PflMI CCAn_nnn'nTGG Cuts at: 0 23 1377 Size: 23 1354 PfoI T'CCnGG_A Cuts at: 0 354 1377 Size: 354 1023 PmlI CAC'GTG Cuts at: 0 753 1377 Size: 753 624 PpuMI rG'GwC_Cy Cuts at: 0 643 921 1093 1377 Size: 643 278 172 284 Fragments arranged by size: 643 284 278 172 PshAI GACnn'nnGTC Cuts at: 0 413 919 1187 1377 Size: 413 506 268 190 Fragments arranged by size: 506 413 268 190 PspOMI G'GGCC_C Cuts at: 0 843 1377 Size: 843 534 PstI C_TGCA'G Cuts at: 0 671 696 1377 Size: 671 25 681 Fragments arranged by size: 681 671 25 PvuII CAG'CTG Cuts at: 0 672 1116 1215 1377 Size: 672 444 99 162 Fragments arranged by size: 672 444 162 99 SanDI GG'GwC_CC Cuts at: 0 643 1377 Size: 643 734 SapI GCTCTTCn'nnn_ Cuts at: 0 162 1377 Size: 162 1215 SexAI A'CCwGG_T Cuts at: 0 1095 1377 Size: 1095 282 SfcI C'TryA_G Cuts at: 0 667 692 1111 1377 Size: 667 25 419 266 Fragments arranged by size: 667 419 266 25 SfiI GGCCn_nnn'nGGCC Cuts at: 0 823 1377 Size: 823 554 SfoI GGC'GCC Cuts at: 0 802 1377 Size: 802 575 SmaI CCC'GGG Cuts at: 0 451 648 806 1377 Size: 451 197 158 571 Fragments arranged by size: 571 451 197 158 SmlI C'TyrA_G Cuts at: 0 365 1377 Size: 365 1012 StuI AGG'CCT Cuts at: 0 59 793 812 1162 1377 Size: 59 734 19 350 215 Fragments arranged by size: 734 350 215 59 19 StyI C'CwwG_G Cuts at: 0 143 794 1377 Size: 143 651 583 Fragments arranged by size: 651 583 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 228 1377 Size: 228 1149 XmaI C'CCGG_G Cuts at: 0 449 646 804 1377 Size: 449 197 158 573 Fragments arranged by size: 573 449 197 158 Enzymes that do cut: AcuI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BstAPI BstYI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII KasI MscI MspA1I NarI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SanDI SapI SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI EcoICRI FalI FseI FspAI HindIII HpaI KpnI MfeI MluI MmeI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI