################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI Aor51HI AscI AseI AsnI Asp700I AviII BamHI BbeI BbrPI BbsI BciVI BfrI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstZ17I CelII CpoI DraI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI Sse8387I SspI SwaI Tru9I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcsI 1471 GAATTC [GA]^AATT[CT] AcyI 570 GACGCC G[GA]^CG[CT]C AhdI 1988 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 204 CCTGAGG CC^T[ACGT]AGG ApoI 1471 GAATTC [GA]^AATT[CT] AspEI 1988 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1515 CCTAGG C^CTAGG BalI 947 TGGCCA TGG^CCA BcgI 1972 CGAAGCAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 663 TGATCA T^GATCA BglII 510 AGATCT A^GATCT BlnI 1515 CCTAGG C^CTAGG BsaHI 570 GACGCC G[GA]^CG[CT]C BsaI 631 GAGACC GGTCTC(1/5) Bsp1407I 558 TGTACA T^GTACA BspDI 1931 ATCGAT AT^CGAT BsrBI 64 CCGCTC CCGCTC(-3/-3) BsrFI 2336 GCCGGT [GA]^CCGG[CT] BsrGI 558 TGTACA T^GTACA BssSI 2264 CACGAG CACGAG(-5/-1) BstAPI 1375 GCACAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1231 TTCGAA TT^CGAA BstEII 2350 GGTGACC G^GT[ACGT]ACC BstPI 2350 GGTGACC G^GT[ACGT]ACC Bsu36I 204 CCTGAGG CC^T[ACGT]AGG Cfr10I 2336 GCCGGT [GA]^CCGG[CT] ClaI 1931 ATCGAT AT^CGAT DraIII 1376 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1394 GACCCTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1983 CCATGG C^C[GA][CT]GG Eam1105I 1988 GACATGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 204 CCTGAGG CC^T[ACGT]AGG EcoO65I 2350 GGTGACC G^GT[ACGT]ACC EcoRI 1471 GAATTC G^AATTC FbaI 663 TGATCA T^GATCA Hin1I 570 GACGCC G[GA]^CG[CT]C MluNI 947 TGGCCA TGG^CCA MscI 947 TGGCCA TGG^CCA NcoI 1983 CCATGG C^CATGG NruI 499 TCGCGA TCG^CGA NspI 1556 GCATGC [GA]CATG^[CT] NspV 1231 TTCGAA TT^CGAA SexAI 806 ACCTGGT A^CC[AT]GGT SfuI 1231 TTCGAA TT^CGAA SmlI 2182 CTCAAG C^T[CT][GA]AG SpeI 774 ACTAGT A^CTAGT SphI 1556 GCATGC GCATG^C SspBI 558 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AccII 39 CGCG CG^CG 500 CGCG Alw44I 1373 GTGCAC G^TGCAC 1843 GTGCAC ApaLI 1373 GTGCAC G^TGCAC 1843 GTGCAC AspI 1324 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2421 GACAGTGTC BbvCI 2097 CCTCAGC CCTCAGC(-5/-2) 1362 GCTGAGG BstUI 39 CGCG CG^CG 500 CGCG EarI 1228 CTCTTC CTCTTC(1/4) 362 GAAGAG HgaI 570 GACGC GACGC(5/10) 1328 GCGTC Ksp632I 1228 CTCTTC CTCTTC(1/4) 362 GAAGAG MvnI 39 CGCG CG^CG 500 CGCG PflFI 1324 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2421 GACAGTGTC PstI 1176 CTGCAG CTGCA^G 1833 CTGCAG Tth111I 1324 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2421 GACAGTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### Acc65I AciI AfaI AluI AlwI AlwNI ApaI Asp718I AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KpnI MaeI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII