(Circular) (Six-base) MAPSORT of: pFN21AE1324.seq Check: 3651 from: 1 to: 7147 pFN21AE1324 7147 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2417 2532 2715 4208 2417 Size: 115 183 1493 5356 Fragments arranged by size: 5356 1493 183 115 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4957 278 Size: 53 83 186 936 3421 2468 Fragments arranged by size: 3421 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4490 1937 Size: 2553 4594 Acc65I G'GTAC_C Cuts at: 2301 4152 4396 4468 2301 Size: 1851 244 72 4980 Fragments arranged by size: 4980 1851 244 72 AclI AA'CG_TT Cuts at: 1563 5274 5647 1563 Size: 3711 373 3063 Fragments arranged by size: 3711 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 2171 2468 3157 5210 6594 6862 1632 Size: 503 36 297 689 2053 1384 268 1917 Fragments arranged by size: 2053 1917 1384 689 503 297 268 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6950 AflIII A'CryG_T Cuts at: 1203 6046 7023 1203 Size: 4843 977 1327 Fragments arranged by size: 4843 1327 977 AgeI A'CCGG_T Cuts at: 5978 5978 Size: 7147 AhdI GACnn_n'nnGTC Cuts at: 3998 5876 3998 Size: 1878 5269 AleI CACnn'nnGTG Cuts at: 1166 2321 2538 1166 Size: 1155 217 5775 Fragments arranged by size: 5775 1155 217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2072 3863 1184 Size: 888 1791 4468 Fragments arranged by size: 4468 1791 888 AlwNI CAG_nnn'CTG Cuts at: 2707 3180 3775 4020 4149 4376 4890 6462 2707 Size: 473 595 245 129 227 514 1572 3392 Fragments arranged by size: 3392 1572 595 514 473 245 227 129 ApaI G_GGCC'C Cuts at: 2029 3196 4261 2029 Size: 1167 1065 4915 Fragments arranged by size: 4915 1167 1065 ApaLI G'TGCA_C Cuts at: 3378 3848 5204 6360 3378 Size: 470 1356 1156 4165 Fragments arranged by size: 4165 1356 1156 470 ApoI r'AATT_y Cuts at: 1493 3476 4456 4682 4834 6943 1493 Size: 1983 980 226 152 2109 1697 Fragments arranged by size: 2109 1983 1697 980 226 152 AseI AT'TA_AT Cuts at: 160 1032 5701 160 Size: 872 4669 1606 Fragments arranged by size: 4669 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7147 AvaI C'yCGr_G Cuts at: 1870 1943 2122 2319 3660 4472 1870 Size: 73 179 197 1341 812 4545 Fragments arranged by size: 4545 1341 812 197 179 73 AvrII C'CTAG_G Cuts at: 3520 3520 Size: 7147 BamHI G'GATC_C Cuts at: 4477 4844 4477 Size: 367 6780 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2301 4042 4139 4152 4344 Size: 325 217 627 514 1741 97 13 192 Cuts at: 4344 4396 4468 5923 618 Size: 52 72 1455 1842 Fragments arranged by size: 1842 1741 1455 627 514 325 217 192 97 72 52 13 BanII G_rGCy'C Cuts at: 1391 2029 2037 2133 2142 2208 2421 3196 3537 Size: 638 8 96 9 66 213 775 341 Cuts at: 3537 3622 3785 4261 4466 1391 Size: 85 163 476 205 4072 Fragments arranged by size: 4072 775 638 476 341 213 205 163 96 85 66 9 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6176 BbvCI CC'TCA_GC Cuts at: 3368 4103 3368 Size: 735 6412 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3966 4000 5338 5372 1903 Size: 34 50 34 1945 34 1338 34 3678 Fragments arranged by size: 3678 1945 1338 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5039 6249 5039 Size: 1210 5937 BclI T'GATC_A Cuts at: 1548 2668 1548 Size: 1120 6027 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2065 3432 4265 Size: 107 122 71 631 605 393 1367 833 Cuts at: 4265 5758 136 Size: 1493 1525 Fragments arranged by size: 1525 1493 1367 833 631 605 393 122 107 71 BglII A'GATC_T Cuts at: 2515 7142 2515 Size: 4627 2520 BlpI GC'TnA_GC Cuts at: 4564 4564 Size: 7147 Bme1580I G_kGCm'C Cuts at: 1790 2029 2545 2875 2908 3196 3382 3852 3967 Size: 239 516 330 33 288 186 470 115 Cuts at: 3967 4045 4162 4261 4349 5208 6364 1790 Size: 78 117 99 88 859 1156 2573 Fragments arranged by size: 2573 1156 859 516 470 330 288 239 186 117 115 99 88 78 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3493 3690 4878 Size: 416 53 83 399 24 2076 197 1188 Cuts at: 4878 5836 442 Size: 958 1753 Fragments arranged by size: 2076 1753 1188 958 416 399 197 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7147 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 2483 2810 3218 3629 3636 3731 4387 Size: 1031 213 327 408 411 7 95 656 Cuts at: 4387 5807 1239 Size: 1420 2579 Fragments arranged by size: 2579 1420 1031 656 411 408 327 213 95 7 Bpu10I CC'TnA_GC Cuts at: 2202 2546 3368 4103 2202 Size: 344 822 735 5246 Fragments arranged by size: 5246 822 735 344 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4172 5272 6137 6435 4172 Size: 1100 865 298 4884 Fragments arranged by size: 4884 1100 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2630 5810 882 Size: 617 1131 3180 2219 Fragments arranged by size: 3180 2219 1131 617 BsaAI yAC'GTr Cuts at: 493 1204 4938 493 Size: 711 3734 2702 Fragments arranged by size: 3734 2702 711 BsaBI GATnn'nnATC Cuts at: 7141 7141 Size: 7147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2576 4954 5336 5971 Size: 53 83 186 936 1043 2378 382 635 Cuts at: 5971 275 Size: 1451 Fragments arranged by size: 2378 1451 1043 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5580 5978 6252 6399 6810 5580 Size: 398 274 147 411 5917 Fragments arranged by size: 5917 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3627 3657 1178 Size: 30 165 30 2224 30 4668 Fragments arranged by size: 4668 2224 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 3025 4046 4091 1186 Size: 922 917 1021 45 4242 Fragments arranged by size: 4242 1021 922 917 45 BseYI C'CCAG_C Cuts at: 2802 2833 2907 3118 3588 3621 3768 3778 3843 Size: 31 74 211 470 33 147 10 65 Cuts at: 3843 3876 4038 6350 6846 2802 Size: 33 162 2312 496 3103 Fragments arranged by size: 3103 2312 496 470 211 162 147 74 65 33 33 31 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2389 3700 4039 4151 1163 Size: 573 653 1311 339 112 4159 Fragments arranged by size: 4159 1311 653 573 339 112 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4597 5358 6386 1540 Size: 275 186 2596 761 1028 2301 Fragments arranged by size: 2596 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2589 3382 3852 5208 5293 6364 2589 Size: 793 470 1356 85 1071 3372 Fragments arranged by size: 3372 1356 1071 793 470 85 BsmI GAATG_Cn' Cuts at: 4658 4751 4658 Size: 93 7054 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2037 2133 2142 2208 2421 2545 Size: 399 239 8 96 9 66 213 124 Cuts at: 2545 2589 2875 2908 3196 3382 3537 3622 3785 Size: 44 286 33 288 186 155 85 163 Cuts at: 3785 3852 3967 4045 4162 4261 4349 4466 5208 Size: 67 115 78 117 99 88 117 742 Cuts at: 5208 5293 6364 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 288 286 239 213 186 163 155 124 117 117 115 99 96 88 85 85 78 67 66 44 33 9 8 BspEI T'CCGG_A Cuts at: 6810 6810 Size: 7147 BspHI T'CATG_A Cuts at: 5036 6766 5036 Size: 1730 5417 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2417 2532 2715 3137 3368 4208 4368 4502 Size: 1573 115 183 422 231 840 160 134 Cuts at: 4502 844 Size: 3489 Fragments arranged by size: 3489 1573 840 422 231 183 160 134 115 BsrBI CCG'CTC Cuts at: 1394 1823 2071 5034 1394 Size: 429 248 2963 3507 Fragments arranged by size: 3507 2963 429 248 BsrDI GCAATG_nn' Cuts at: 65 1248 5642 5816 65 Size: 1183 4394 174 1396 Fragments arranged by size: 4394 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4341 5791 5978 1700 Size: 110 2531 1450 187 2869 Fragments arranged by size: 2869 2531 1450 187 110 BsrGI T'GTAC_A Cuts at: 96 2563 96 Size: 2467 4680 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7147 BssSI C'ACGA_G Cuts at: 4269 5207 6219 4269 Size: 938 1012 5197 Fragments arranged by size: 5197 1012 938 BstAPI GCAn_nnn'nTGC Cuts at: 794 3386 794 Size: 2592 4555 BstBI TT'CG_AA Cuts at: 3237 5951 3237 Size: 2714 4433 BstEII G'GTnAC_C Cuts at: 1184 4355 1184 Size: 3171 3976 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2312 2647 3022 3995 2011 Size: 301 335 375 973 5163 Fragments arranged by size: 5163 973 375 335 301 BstYI r'GATC_y Cuts at: 1972 2515 3793 3952 4477 4844 5229 5246 6698 Size: 543 1278 159 525 367 385 17 1452 Cuts at: 6698 6784 6796 7142 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1278 543 525 385 367 346 159 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2210 2210 Size: 7147 BtgI C'CryG_G Cuts at: 513 1065 3988 513 Size: 552 2923 3672 Fragments arranged by size: 3672 2923 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6408 Fragments arranged by size: 6408 683 56 BtsI GCAGTG_nn' Cuts at: 3311 3446 3599 3871 4018 4319 4663 5457 5477 Size: 135 153 272 147 301 344 794 20 Cuts at: 5477 3311 Size: 4981 Fragments arranged by size: 4981 794 344 301 272 153 147 135 20 ClaI AT'CG_AT Cuts at: 1554 3937 1554 Size: 2383 4764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3679 3714 529 Size: 35 3115 35 3962 Fragments arranged by size: 3962 3115 35 35 DraI TTT'AAA Cuts at: 4451 4451 Size: 7147 DraIII CAC_nnn'GTG Cuts at: 3386 3386 Size: 7147 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3405 6154 809 Size: 507 2089 2749 1802 Fragments arranged by size: 2749 2089 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2066 2952 4238 Size: 54 1425 50 275 18 236 886 1286 Cuts at: 4238 4594 5483 7112 8 Size: 356 889 1629 43 Fragments arranged by size: 1629 1425 1286 889 886 356 275 236 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4594 1537 Size: 275 2782 4090 Fragments arranged by size: 4090 2782 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2362 3239 5077 1353 Size: 1009 877 1838 3423 Fragments arranged by size: 3423 1838 1009 877 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5734 6108 6254 387 Size: 1064 138 4145 374 146 1280 Fragments arranged by size: 4145 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2171 2270 2468 2483 2810 3157 Size: 393 503 36 99 198 15 327 347 Cuts at: 3157 3218 3629 3636 3731 4387 5210 5807 6594 Size: 61 411 7 95 656 823 597 787 Cuts at: 6594 6862 1239 Size: 268 1524 Fragments arranged by size: 1524 823 787 656 597 503 411 393 347 327 268 198 99 95 61 36 15 7 EcoNI CCTnn'n_nnAGG Cuts at: 2177 2465 3453 2177 Size: 288 988 5871 Fragments arranged by size: 5871 988 288 EcoO109I rG'GnC_Cy Cuts at: 2026 2119 2146 2471 2479 2489 3193 3611 3765 Size: 93 27 325 8 10 704 418 154 Cuts at: 3765 4193 4244 4257 4258 2026 Size: 428 51 13 1 4915 Fragments arranged by size: 4915 704 428 418 325 154 93 51 27 13 10 8 1 EcoRI G'AATT_C Cuts at: 3476 4456 4834 3476 Size: 980 378 5789 Fragments arranged by size: 5789 980 378 FspI TGC'GCA Cuts at: 5653 5653 Size: 7147 HaeII r_GCGC'y Cuts at: 1678 2018 2605 4415 6294 1678 Size: 340 587 1810 1879 2531 Fragments arranged by size: 2531 1879 1810 587 340 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3757 3789 3984 4016 5788 Size: 32 135 32 2185 32 195 32 1772 Cuts at: 5788 5820 5862 5894 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2185 1772 195 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4491 4737 669 Size: 988 281 2553 246 3079 Fragments arranged by size: 3079 2553 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7147 HpaI GTT'AAC Cuts at: 4737 4737 Size: 7147 KpnI G_GTAC'C Cuts at: 2305 4156 4400 4472 2305 Size: 1851 244 72 4980 Fragments arranged by size: 4980 1851 244 72 MfeI C'AATT_G Cuts at: 4746 4746 Size: 7147 MluI A'CGCG_T Cuts at: 7023 7023 Size: 7147 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2202 4448 6261 6445 1305 Size: 897 2246 1813 184 2007 Fragments arranged by size: 2246 2007 1813 897 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2954 10 Size: 54 1425 343 1122 4203 Fragments arranged by size: 4203 1425 1122 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 2538 2901 3970 3993 5105 5464 Size: 648 1155 217 363 1069 23 1112 359 Cuts at: 5464 5623 518 Size: 159 2042 Fragments arranged by size: 2042 1155 1112 1069 648 363 359 217 159 23 MspA1I CmG'CkG Cuts at: 1606 2837 2911 3228 3367 3421 3629 3843 4563 Size: 1231 74 317 139 54 208 214 720 Cuts at: 4563 5240 6388 6633 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1231 1148 720 677 317 245 214 208 139 74 54 NcoI C'CATG_G Cuts at: 513 1065 3988 513 Size: 552 2923 3672 Fragments arranged by size: 3672 2923 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7147 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7147 NotI GC'GGCC_GC Cuts at: 4594 4594 Size: 7147 NruI TCG'CGA Cuts at: 2506 2506 Size: 7147 NspI r_CATG'y Cuts at: 1132 1229 3565 4505 6050 1132 Size: 97 2336 940 1545 2229 Fragments arranged by size: 2336 2229 1545 940 97 PasI CC'CwG_GG Cuts at: 1348 2029 2593 4336 4370 1348 Size: 681 564 1743 34 4125 Fragments arranged by size: 4125 1743 681 564 34 PciI A'CATG_T Cuts at: 6046 6046 Size: 7147 PflMI CCAn_nnn'nTGG Cuts at: 1108 3052 3064 3949 4149 4376 1108 Size: 1944 12 885 200 227 3879 Fragments arranged by size: 3879 1944 885 227 200 12 PfoI T'CCnGG_A Cuts at: 2247 3210 3321 3789 2247 Size: 963 111 468 5605 Fragments arranged by size: 5605 963 468 111 PmeI GTTT'AAAC Cuts at: 4451 4451 Size: 7147 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5216 5248 6729 6761 5216 Size: 32 1481 32 5602 Fragments arranged by size: 5602 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2146 2479 2489 3611 3765 4193 4244 2146 Size: 333 10 1122 154 428 51 5049 Fragments arranged by size: 5049 1122 428 333 154 51 10 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7147 PsiI TTA'TAA Cuts at: 4717 4717 Size: 7147 PspOMI G'GGCC_C Cuts at: 2025 3192 4257 2025 Size: 1167 1065 4915 Fragments arranged by size: 4915 1167 1065 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2342 2374 2342 Size: 32 7115 PstI C_TGCA'G Cuts at: 830 3185 3842 4499 830 Size: 2355 657 657 3478 Fragments arranged by size: 3478 2355 657 657 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7147 PvuII CAG'CTG Cuts at: 2837 2911 3228 3367 3421 3843 2837 Size: 74 317 139 54 422 6141 Fragments arranged by size: 6141 422 317 139 74 54 SalI G'TCGA_C Cuts at: 1936 4489 1936 Size: 2553 4594 SanDI GG'GwC_CC Cuts at: 4193 4193 Size: 7147 SbfI CC_TGCA'GG Cuts at: 4499 4499 Size: 7147 ScaI AGT'ACT Cuts at: 5395 7107 5395 Size: 1712 5435 SexAI A'CCwGG_T Cuts at: 2811 2811 Size: 7147 SfcI C'TryA_G Cuts at: 826 1045 2258 3181 3838 4495 5630 6311 6502 Size: 219 1213 923 657 657 1135 681 191 Cuts at: 6502 826 Size: 1471 Fragments arranged by size: 1471 1213 1135 923 681 657 657 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7147 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7147 SmaI CCC'GGG Cuts at: 1872 4474 1872 Size: 2602 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4187 5251 6152 6414 820 Size: 197 926 2244 1064 901 262 1553 Fragments arranged by size: 2244 1553 1064 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4938 493 Size: 4445 2702 SpeI A'CTAG_T Cuts at: 152 2779 152 Size: 2627 4520 SphI G_CATG'C Cuts at: 1132 3565 4505 1132 Size: 2433 940 3774 Fragments arranged by size: 3774 2433 940 SspI AAT'ATT Cuts at: 5 52 5071 5 Size: 47 5019 2081 Fragments arranged by size: 5019 2081 47 StuI AGG'CCT Cuts at: 1517 2795 3143 3165 1517 Size: 1278 348 22 5499 Fragments arranged by size: 5499 1278 348 22 StyI C'CwwG_G Cuts at: 513 1065 2133 3520 3988 4310 4586 513 Size: 552 1068 1387 468 322 276 3074 Fragments arranged by size: 3074 1387 1068 552 468 322 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2383 3835 5174 5191 5344 5529 5974 Size: 1255 803 1452 1339 17 153 185 445 Cuts at: 5974 325 Size: 1498 Fragments arranged by size: 1498 1452 1339 1255 803 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2563 4206 5393 Size: 274 80 33 51 1443 586 1643 1187 Cuts at: 5393 7105 96 Size: 1712 138 Fragments arranged by size: 1712 1643 1443 1187 586 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2791 2823 3627 3659 7107 7139 2791 Size: 32 804 32 3448 32 2799 Fragments arranged by size: 3448 2799 804 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3332 4429 1316 Size: 2016 1097 4034 Fragments arranged by size: 4034 2016 1097 XbaI T'CTAG_A Cuts at: 4483 4483 Size: 7147 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2295 2915 3019 3083 2295 Size: 620 104 64 6359 Fragments arranged by size: 6359 620 104 64 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7147 XmaI C'CCGG_G Cuts at: 1870 4472 1870 Size: 2602 4545 XmnI GAAnn'nnTTC Cuts at: 5276 5276 Size: 7147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4955 276 Size: 53 83 186 936 3421 2468 Fragments arranged by size: 3421 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI