(Linear) (Six-base) MAPSORT of: KIEE1324.seq Check: 3179 from: 1 to: 2442 KIEE1324.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 412 527 710 2203 2442 Size: 412 115 183 1493 239 Fragments arranged by size: 1493 412 239 183 115 Acc65I G'GTAC_C Cuts at: 0 296 2147 2391 2442 Size: 296 1851 244 51 Fragments arranged by size: 1851 296 244 51 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 166 463 1152 2442 Size: 130 36 297 689 1290 Fragments arranged by size: 1290 689 297 130 36 AhdI GACnn_n'nnGTC Cuts at: 0 1993 2442 Size: 1993 449 AleI CACnn'nnGTG Cuts at: 0 316 533 2442 Size: 316 217 1909 Fragments arranged by size: 1909 316 217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 67 1858 2442 Size: 67 1791 584 Fragments arranged by size: 1791 584 67 AlwNI CAG_nnn'CTG Cuts at: 0 702 1175 1770 2015 2144 2371 2442 Size: 702 473 595 245 129 227 71 Fragments arranged by size: 702 595 473 245 227 129 71 ApaI G_GGCC'C Cuts at: 0 24 1191 2256 2442 Size: 24 1167 1065 186 Fragments arranged by size: 1167 1065 186 24 ApaLI G'TGCA_C Cuts at: 0 1373 1843 2442 Size: 1373 470 599 Fragments arranged by size: 1373 599 470 ApoI r'AATT_y Cuts at: 0 1471 2442 Size: 1471 971 AvaI C'yCGr_G Cuts at: 0 117 314 1655 2442 Size: 117 197 1341 787 Fragments arranged by size: 1341 787 197 117 AvrII C'CTAG_G Cuts at: 0 1515 2442 Size: 1515 927 BanI G'GyrC_C Cuts at: 0 296 2037 2134 2147 2339 2391 2442 Size: 296 1741 97 13 192 52 51 Fragments arranged by size: 1741 296 192 97 52 51 13 BanII G_rGCy'C Cuts at: 0 24 32 128 137 203 416 1191 1532 Size: 24 8 96 9 66 213 775 341 Cuts at: 1532 1617 1780 2256 2442 Size: 85 163 476 186 Fragments arranged by size: 775 476 341 213 186 163 96 85 66 24 9 8 BbvCI CC'TCA_GC Cuts at: 0 1363 2098 2442 Size: 1363 735 344 Fragments arranged by size: 1363 735 344 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1961 1995 2442 Size: 1961 34 447 Fragments arranged by size: 1961 447 34 BclI T'GATC_A Cuts at: 0 663 2442 Size: 663 1779 BglI GCCn_nnn'nGGC Cuts at: 0 60 1427 2260 2442 Size: 60 1367 833 182 Fragments arranged by size: 1367 833 182 60 BglII A'GATC_T Cuts at: 0 510 2442 Size: 510 1932 Bme1580I G_kGCm'C Cuts at: 0 24 540 870 903 1191 1377 1847 1962 Size: 24 516 330 33 288 186 470 115 Cuts at: 1962 2040 2157 2256 2344 2442 Size: 78 117 99 88 98 Fragments arranged by size: 516 470 330 288 186 117 115 99 98 88 78 33 24 BmrI ACTGGGnnnn_n' Cuts at: 0 1488 1685 2442 Size: 1488 197 757 Fragments arranged by size: 1488 757 197 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 478 805 1213 1624 1631 1726 2382 Size: 265 213 327 408 411 7 95 656 Cuts at: 2382 2442 Size: 60 Fragments arranged by size: 656 411 408 327 265 213 95 60 7 Bpu10I CC'TnA_GC Cuts at: 0 197 541 1363 2098 2442 Size: 197 344 822 735 344 Fragments arranged by size: 822 735 344 344 197 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2167 2442 Size: 2167 275 BsaI GGTCTCn'nnnn_ Cuts at: 0 625 2442 Size: 625 1817 BsaHI Gr'CG_yC Cuts at: 0 571 2442 Size: 571 1871 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1622 1652 2442 Size: 1622 30 790 Fragments arranged by size: 1622 790 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 103 1020 2041 2086 2442 Size: 103 917 1021 45 356 Fragments arranged by size: 1021 917 356 103 45 BseYI C'CCAG_C Cuts at: 0 797 828 902 1113 1583 1616 1763 1773 Size: 797 31 74 211 470 33 147 10 Cuts at: 1773 1838 1871 2033 2442 Size: 65 33 162 409 Fragments arranged by size: 797 470 409 211 162 147 74 65 33 33 31 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 384 1695 2034 2146 2442 Size: 384 1311 339 112 296 Fragments arranged by size: 1311 384 339 296 112 BsiHKAI G_wGCw'C Cuts at: 0 584 1377 1847 2442 Size: 584 793 470 595 Fragments arranged by size: 793 595 584 470 Bsp1286I G_dGCh'C Cuts at: 0 24 32 128 137 203 416 540 584 Size: 24 8 96 9 66 213 124 44 Cuts at: 584 870 903 1191 1377 1532 1617 1780 1847 Size: 286 33 288 186 155 85 163 67 Cuts at: 1847 1962 2040 2157 2256 2344 2442 Size: 115 78 117 99 88 98 Fragments arranged by size: 288 286 213 186 163 155 124 117 115 99 98 96 88 85 78 67 66 44 33 24 9 8 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 412 527 710 1132 1363 2203 2363 2442 Size: 412 115 183 422 231 840 160 79 Fragments arranged by size: 840 422 412 231 183 160 115 79 BsrBI CCG'CTC Cuts at: 0 66 2442 Size: 66 2376 BsrFI r'CCGG_y Cuts at: 0 2336 2442 Size: 2336 106 BsrGI T'GTAC_A Cuts at: 0 558 2442 Size: 558 1884 BssSI C'ACGA_G Cuts at: 0 2264 2442 Size: 2264 178 BstAPI GCAn_nnn'nTGC Cuts at: 0 1381 2442 Size: 1381 1061 BstBI TT'CG_AA Cuts at: 0 1232 2442 Size: 1232 1210 BstEII G'GTnAC_C Cuts at: 0 2350 2442 Size: 2350 92 BstXI CCAn_nnnn'nTGG Cuts at: 0 307 642 1017 1990 2442 Size: 307 335 375 973 452 Fragments arranged by size: 973 452 375 335 307 BstYI r'GATC_y Cuts at: 0 510 1788 1947 2442 Size: 510 1278 159 495 Fragments arranged by size: 1278 510 495 159 Bsu36I CC'TnA_GG Cuts at: 0 205 2442 Size: 205 2237 BtgI C'CryG_G Cuts at: 0 1983 2442 Size: 1983 459 BtsI GCAGTG_nn' Cuts at: 0 1306 1441 1594 1866 2013 2314 2442 Size: 1306 135 153 272 147 301 128 Fragments arranged by size: 1306 301 272 153 147 135 128 ClaI AT'CG_AT Cuts at: 0 1932 2442 Size: 1932 510 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1674 1709 2442 Size: 1674 35 733 Fragments arranged by size: 1674 733 35 DraIII CAC_nnn'GTG Cuts at: 0 1381 2442 Size: 1381 1061 DrdI GACnn_nn'nnGTC Cuts at: 0 1400 2442 Size: 1400 1042 EaeI y'GGCC_r Cuts at: 0 61 947 2233 2442 Size: 61 886 1286 209 Fragments arranged by size: 1286 886 209 61 EarI CTCTTCn'nnn_ Cuts at: 0 357 1234 2442 Size: 357 877 1208 Fragments arranged by size: 1208 877 357 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 166 265 463 478 805 1152 1213 Size: 130 36 99 198 15 327 347 61 Cuts at: 1213 1624 1631 1726 2382 2442 Size: 411 7 95 656 60 Fragments arranged by size: 656 411 347 327 198 130 99 95 61 60 36 15 7 EcoNI CCTnn'n_nnAGG Cuts at: 0 172 460 1448 2442 Size: 172 288 988 994 Fragments arranged by size: 994 988 288 172 EcoO109I rG'GnC_Cy Cuts at: 0 21 114 141 466 474 484 1188 1606 Size: 21 93 27 325 8 10 704 418 Cuts at: 1606 1760 2188 2239 2252 2253 2442 Size: 154 428 51 13 1 189 Fragments arranged by size: 704 428 418 325 189 154 93 51 27 21 13 10 8 1 EcoRI G'AATT_C Cuts at: 0 1471 2442 Size: 1471 971 HaeII r_GCGC'y Cuts at: 0 13 600 2410 2442 Size: 13 587 1810 32 Fragments arranged by size: 1810 587 32 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1752 1784 1979 2011 2442 Size: 1752 32 195 32 431 Fragments arranged by size: 1752 431 195 32 32 KpnI G_GTAC'C Cuts at: 0 300 2151 2395 2442 Size: 300 1851 244 47 Fragments arranged by size: 1851 300 244 47 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 197 2442 Size: 197 2245 MscI TGG'CCA Cuts at: 0 949 2442 Size: 949 1493 MslI CAynn'nnrTG Cuts at: 0 316 533 896 1965 1988 2442 Size: 316 217 363 1069 23 454 Fragments arranged by size: 1069 454 363 316 217 23 MspA1I CmG'CkG Cuts at: 0 832 906 1223 1362 1416 1624 1838 2442 Size: 832 74 317 139 54 208 214 604 Fragments arranged by size: 832 604 317 214 208 139 74 54 NcoI C'CATG_G Cuts at: 0 1983 2442 Size: 1983 459 NruI TCG'CGA Cuts at: 0 501 2442 Size: 501 1941 NspI r_CATG'y Cuts at: 0 1560 2442 Size: 1560 882 PasI CC'CwG_GG Cuts at: 0 24 588 2331 2365 2442 Size: 24 564 1743 34 77 Fragments arranged by size: 1743 564 77 34 24 PflMI CCAn_nnn'nTGG Cuts at: 0 1047 1059 1944 2144 2371 2442 Size: 1047 12 885 200 227 71 Fragments arranged by size: 1047 885 227 200 71 12 PfoI T'CCnGG_A Cuts at: 0 242 1205 1316 1784 2442 Size: 242 963 111 468 658 Fragments arranged by size: 963 658 468 242 111 PpuMI rG'GwC_Cy Cuts at: 0 141 474 484 1606 1760 2188 2239 2442 Size: 141 333 10 1122 154 428 51 203 Fragments arranged by size: 1122 428 333 203 154 141 51 10 PspOMI G'GGCC_C Cuts at: 0 20 1187 2252 2442 Size: 20 1167 1065 190 Fragments arranged by size: 1167 1065 190 20 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 337 369 2442 Size: 337 32 2073 Fragments arranged by size: 2073 337 32 PstI C_TGCA'G Cuts at: 0 1180 1837 2442 Size: 1180 657 605 Fragments arranged by size: 1180 657 605 PvuII CAG'CTG Cuts at: 0 832 906 1223 1362 1416 1838 2442 Size: 832 74 317 139 54 422 604 Fragments arranged by size: 832 604 422 317 139 74 54 SanDI GG'GwC_CC Cuts at: 0 2188 2442 Size: 2188 254 SexAI A'CCwGG_T Cuts at: 0 806 2442 Size: 806 1636 SfcI C'TryA_G Cuts at: 0 253 1176 1833 2442 Size: 253 923 657 609 Fragments arranged by size: 923 657 609 253 SmlI C'TyrA_G Cuts at: 0 2182 2442 Size: 2182 260 SpeI A'CTAG_T Cuts at: 0 774 2442 Size: 774 1668 SphI G_CATG'C Cuts at: 0 1560 2442 Size: 1560 882 StuI AGG'CCT Cuts at: 0 790 1138 1160 2442 Size: 790 348 22 1282 Fragments arranged by size: 1282 790 348 22 StyI C'CwwG_G Cuts at: 0 128 1515 1983 2305 2442 Size: 128 1387 468 322 137 Fragments arranged by size: 1387 468 322 137 128 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 378 1830 2442 Size: 378 1452 612 Fragments arranged by size: 1452 612 378 TatI w'GTAC_w Cuts at: 0 558 2201 2442 Size: 558 1643 241 Fragments arranged by size: 1643 558 241 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 786 818 1622 1654 2442 Size: 786 32 804 32 788 Fragments arranged by size: 804 788 786 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1327 2424 2442 Size: 1327 1097 18 Fragments arranged by size: 1327 1097 18 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 290 910 1014 1078 2442 Size: 290 620 104 64 1364 Fragments arranged by size: 1364 620 290 104 64 Enzymes that do cut: AarI Acc65I AcuI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbvCI BcgI BclI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KpnI MmeI MscI MslI MspA1I NcoI NruI NspI PasI PflMI PfoI PpuMI PspOMI PsrI PstI PvuII SanDI SexAI SfcI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AloI AscI AseI AsiSI BaeI BamHI BbeI BbsI BciVI BfrBI BlpI BmgBI BmtI BplI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BstZ17I BtgZI DraI EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI