################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII Hin1I HincII HindII HinfI HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MseI MunI MvnI MwoI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 457 GTAGAC GT^[AC][GT]AC AcsI 49 AAATTC [GA]^AATT[CT] AlwI 489 GATCC GGATC(4/5) AlwNI 23 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 49 AAATTC [GA]^AATT[CT] AspHI 433 GAGCAC G[AT]GC[AT]^C AvaI 532 CTCGGG C^[CT]CG[GA]G BcnI 408 CCCGG CC^[GC]GG BmyI 433 GAGCAC G[AGT]GC[ACT]^C BsaI 529 GGTCTC GGTCTC(1/5) BseRI 302 CTCCTC GAGGAG(10/8) BsiHKAI 433 GAGCAC G[AT]GC[AT]^C BsmAI 530 GTCTC GTCTC(1/5) BsoBI 532 CTCGGG C^[CT]CG[GA]G Bsp1286I 433 GAGCAC G[AGT]GC[ACT]^C BsrBI 243 CCGCTC CCGCTC(-3/-3) BstYI 488 AGATCC [GA]^GATC[CT] CfoI 448 GCGC GCG^C DdeI 158 CTGAG C^T[ACGT]AG DrdI 190 GACATTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 539 CCATGG C^C[GA][CT]GG EcoT14I 539 CCATGG C^C[AT][AT]GG FauI 372 GCGGG CCCGC(4/6) HapII 409 CCGG C^CGG HhaI 448 GCGC GCG^C HinP1I 448 GCGC G^CGC HindIII 115 AAGCTT A^AGCTT HpaII 409 CCGG C^CGG HphI 200 TCACC GGTGA(8/7) MflI 488 AGATCC [GA]^GATC[CT] MslI 420 CACCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 23 CAGCTG C[AC]G^C[GT]G MspI 409 CCGG C^CGG NciI 408 CCCGG CC^[GC]GG NcoI 539 CCATGG C^CATGG PvuII 23 CAGCTG CAG^CTG SmlI 466 CTCAAG C^T[CT][GA]AG StyI 539 CCATGG C^C[AT][AT]GG TaqI 485 TCGA T^CGA Tsp509I 50 AATT ^AATT TthHB8I 485 TCGA T^CGA XcmI 539 CCATGGCTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 488 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AvaII 381 GGACC G^G[AT]CC 554 GGACC BalI 332 TGGCCA TGG^CCA 500 TGGCCA BsgI 29 CTGCAC GTGCAG(16/14) 259 CTGCAC BsmFI 380 GGGAC GGGAC(10/14) 553 GGGAC BsrI 253 CCAGT ACTGG(1/-1) 335 CCAGT Cac8I 372 GCGGGC GC[ACGT]^[ACGT]GC 389 GCAAGC DpnII 441 GATC ^GATC 489 GATC HgaI 214 GACGC GACGC(5/10) 365 GCGTC MboI 441 GATC ^GATC 489 GATC MluNI 332 TGGCCA TGG^CCA 500 TGGCCA MscI 332 TGGCCA TGG^CCA 500 TGGCCA NdeII 441 GATC ^GATC 489 GATC SapI 237 GAAGAGC GCTCTTC(1/4) 399 GCTCTTC Sau3AI 441 GATC ^GATC 489 GATC SfaNI 310 GATGC GCATC(5/9) 341 GCATC SfcI 177 CTACAG C^T[GA][CT]AG 207 CTACAG StuI 11 AGGCCT AGG^CCT 470 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BspMI BssKI BstNI BstXI Cfr13I EaeI EarI EcoRII Fnu4HI HaeIII ItaI Ksp632I MaeIII MboII MnlI MvaI NlaIII NlaIV Sau96I ScrFI TseI Tsp45I TspRI