################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsoBI BspDI BspEI BspLU11I BspMI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvaI MvnI NarI NdeI NdeII NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmeI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tsp45I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 184 GAATTT [GA]^AATT[CT] AfaI 243 GTAC GT^AC AflIII 239 ACGTGT A^C[GA][CT]GT ApoI 184 GAATTT [GA]^AATT[CT] AspI 458 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 165 GGCACC G^G[CT][GA]CC BbrPI 238 CACGTG CAC^GTG BfaI 5 CTAG C^TAG BpmI 103 CTGGAG CTGGAG(16/14) BsaAI 238 CACGTG [CT]AC^GT[GA] BsmI 75 GAATGC GAATGC(1/-1) Bsp1407I 242 TGTACA T^GTACA BspHI 113 TCATGA T^CATGA BsrBI 106 GAGCGG CCGCTC(-3/-3) BsrFI 220 GCCGGC [GA]^CCGG[CT] BsrGI 242 TGTACA T^GTACA Cfr10I 220 GCCGGC [GA]^CCGG[CT] DdeI 299 CTGAG C^T[ACGT]AG DraII 456 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 456 AGGACCC [GA]G^G[ACGT]CC[CT] HgaI 57 GACGC GACGC(5/10) HphI 539 TCACC GGTGA(8/7) MaeI 5 CTAG C^TAG MaeII 239 ACGT A^CGT MseI 400 TTAA T^TAA NaeI 220 GCCGGC GCC^GGC NgoMIV 220 GCCGGC G^CCGGC NheI 4 GCTAGC G^CTAGC PflFI 458 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 238 CACGTG CAC^GTG PmlI 238 CACGTG CAC^GTG PpuMI 456 AGGACCC [GA]G^G[AT]CC[CT] PshAI 57 GACGCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 356 CTGCAG CTGCA^G RcaI 113 TCATGA T^CATGA RsaI 243 GTAC GT^AC SfcI 356 CTGCAG C^T[GA][CT]AG SmlI 97 CTCAAG C^T[CT][GA]AG SspBI 242 TGTACA T^GTACA TaqI 171 TCGA T^CGA Tru9I 400 TTAA T^TAA TspRI 225 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 458 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 171 TCGA T^CGA XcmI 533 CCAGTTTCACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AvaII 457 GGACC G^G[AT]CC 482 GGTCC BbvI 488 GCAGC GCAGC(8/12) 355 GCTGC BcnI 123 CCCGG CC^[GC]GG 460 CCCGG BmyI 164 GGGCAC G[AGT]GC[ACT]^C 529 GTGCCC Bsp1286I 164 GGGCAC G[AGT]GC[ACT]^C 529 GTGCCC BssKI 123 CCCGG ^CC[ACGT]GG 460 CCCGG BstF5I 66 CATCC GGATG(2/0) 288 GGATG Cac8I 4 GCTAGC GC[ACGT]^[ACGT]GC 220 GCCGGC DsaI 293 CCATGG C^C[GA][CT]GG 542 CCATGG FokI 66 CATCC GGATG(9/13) 288 GGATG HaeII 377 AGCGCC [GA]GCGC^[CT] 389 AGCGCC HaeIII 38 GGCC GG^CC 219 GGCC NciI 123 CCCGG CC^[GC]GG 460 CCCGG NcoI 293 CCATGG C^CATGG 542 CCATGG ScrFI 123 CCCGG CC^[ACGT]GG 460 CCCGG SfaNI 289 GATGC GCATC(5/9) 502 GCATC TseI 355 GCTGC G^C[AT]GC 488 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AluI BsaJI BseRI BsrI CfoI Cfr13I EcoT14I Fnu4HI HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MwoI NlaIII NlaIV Sau96I StyI Tsp509I