################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AvrII BcgI BciVI BclI BfrI BlnI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SbfI SfiI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 2626 GACGTC GACGT^C AccI 510 GTCGAC GT^[AC][GT]AC AccIII 326 TCCGGA T^CCGGA AcsI 3319 AAATTC [GA]^AATT[CT] AflIII 1805 ACGTGT A^C[GA][CT]GT Aor51HI 2681 AGCGCT AGC^GCT ApoI 3319 AAATTC [GA]^AATT[CT] Asp700I 705 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2852 TGCGCA TGC^GCA BbrPI 1153 CACGTG CAC^GTG BglI 2791 GCCCTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1332 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 326 TCCGGA [AT]^CCGG[AT] BseAI 326 TCCGGA T^CCGGA BsmI 2344 GAATGC GAATGC(1/-1) BspEI 326 TCCGGA T^CCGGA BssHII 3076 GCGCGC G^CGCGC BstEII 3264 GGTGACC G^GT[ACGT]ACC BstPI 3264 GGTGACC G^GT[ACGT]ACC BstXI 2405 CCACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EagI 1280 CGGCCG C^GGCCG EclXI 1280 CGGCCG C^GGCCG Eco47III 2681 AGCGCT AGC^GCT Eco52I 1280 CGGCCG C^GGCCG EcoO65I 3264 GGTGACC G^GT[ACGT]ACC FspI 2852 TGCGCA TGC^GCA KspI 1105 CCGCGG CCGC^GG MamI 1332 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 326 TCCGGA T^CCGGA MseI 536 TTAA T^TAA NspI 1487 GCATGC [GA]CATG^[CT] PaeR7I 787 CTCGAG C^TCGAG PmaCI 1153 CACGTG CAC^GTG PmlI 1153 CACGTG CAC^GTG SacI 3328 GAGCTC GAGCT^C SacII 1105 CCGCGG CCGC^GG SalI 510 GTCGAC G^TCGAC SapI 3003 GCTCTTC GCTCTTC(1/4) ScaI 3158 AGTACT AGT^ACT SgrAI 2651 CACCGGCG C[GA]^CCGG[CT]G SmaI 1226 CCCGGG CCC^GGG SphI 1487 GCATGC GCATG^C StuI 78 AGGCCT AGG^CCT TfiI 345 GAATC G^A[AT]TC Tru9I 536 TTAA T^TAA Tsp509I 3320 AATT ^AATT XcmI 62 CCATGGCTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 787 CTCGAG C^TCGAG XmaCI 1226 CCCGGG C^CCGGG XmaI 1226 CCCGGG C^CCGGG XmnI 705 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AocI 837 CCTGAGG CC^T[ACGT]AGG 1846 CCTGAGG ApaI 376 GGGCCC GGGCC^C 928 GGGCCC BamHI 1349 GGATCC G^GATCC 2324 GGATCC BbeI 2293 GGCGCC GGCGC^C 2401 GGCGCC BlpI 1212 GCTGAGC GC^T[ACGT]AGC 1734 GCTCAGC Bpu1102I 1212 GCTGAGC GC^T[ACGT]AGC 1734 GCTCAGC BsaI 3065 GAGACC GGTCTC(1/5) 1992 GGTCTC BsiEI 512 CGACCG CG[GA][CT]^CG 1280 CGGCCG BspHI 1982 TCATGA T^CATGA 3440 TCATGA BspMI 2966 ACCTGC ACCTGC(4/8) 716 GCAGGT BsrDI 177 CATTGC GCAATG(2/0) 3339 CATTGC BstAPI 1491 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2490 GCACCGGCTGC Bsu36I 837 CCTGAGG CC^T[ACGT]AGG 1846 CCTGAGG CelII 1212 GCTGAGC GC^T[ACGT]AGC 1734 GCTCAGC DrdI 3100 GACAGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3459 GACAGAGGCGTC EarI 226 CTCTTC CTCTTC(1/4) 3004 CTCTTC Eco81I 837 CCTGAGG CC^T[ACGT]AGG 1846 CCTGAGG EcoNI 603 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3129 CCTAGCCAAGG HincII 510 GTCGAC GT[CT]^[GA]AC 1750 GTTGAC HindII 510 GTCGAC GT[CT]^[GA]AC 1750 GTTGAC KasI 2293 GGCGCC G^GCGCC 2401 GGCGCC Ksp632I 226 CTCTTC CTCTTC(1/4) 3004 CTCTTC NaeI 659 GCCGGC GCC^GGC 1092 GCCGGC NarI 2293 GGCGCC GG^CGCC 2401 GGCGCC NgoMIV 659 GCCGGC G^CCGGC 1092 GCCGGC PpuMI 1841 GGGACCC [GA]G^G[AT]CC[CT] 1871 AGGACCC PshAI 1933 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3412 GACAAATGTC RcaI 1982 TCATGA T^CATGA 3440 TCATGA SexAI 2186 ACCTGGT A^CC[AT]GGT 2352 ACCAGGT SfoI 2293 GGCGCC GGC^GCC 2401 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII