(Circular) (Six-base) MAPSORT of: pFN21AE1350.seq Check: 1518 from: 1 to: 8266 pFN21AE1350 8266 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4635 6076 278 Size: 53 83 186 936 3099 1441 2468 Fragments arranged by size: 3099 2468 1441 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2516 5609 1937 Size: 579 3093 4594 Fragments arranged by size: 4594 3093 579 Acc65I G'GTAC_C Cuts at: 5587 5587 Size: 8266 AclI AA'CG_TT Cuts at: 1563 6393 6766 1563 Size: 4830 373 3063 Fragments arranged by size: 4830 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 5243 5531 5532 6329 7713 7981 1632 Size: 476 3135 288 1 797 1384 268 1917 Fragments arranged by size: 3135 1917 1384 797 476 288 268 1 AfeI AGC'GCT Cuts at: 4688 4688 Size: 8266 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8069 AflIII A'CryG_T Cuts at: 1203 3810 7165 8142 1203 Size: 2607 3355 977 1327 Fragments arranged by size: 3355 2607 1327 977 AgeI A'CCGG_T Cuts at: 7097 7097 Size: 8266 AhdI GACnn_n'nnGTC Cuts at: 6995 6995 Size: 8266 AleI CACnn'nnGTG Cuts at: 1166 3158 1166 Size: 1992 6274 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2744 4607 5108 1184 Size: 1560 1863 501 4342 Fragments arranged by size: 4342 1863 1560 501 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5219 5251 5219 Size: 32 8234 AlwNI CAG_nnn'CTG Cuts at: 2912 2966 4156 4549 5005 6009 7581 2912 Size: 54 1190 393 456 1004 1572 3597 Fragments arranged by size: 3597 1572 1190 1004 456 393 54 ApaI G_GGCC'C Cuts at: 2385 2937 2385 Size: 552 7714 ApaLI G'TGCA_C Cuts at: 6323 7479 6323 Size: 1156 7110 ApoI r'AATT_y Cuts at: 1493 5324 5575 5801 5953 8062 1493 Size: 3831 251 226 152 2109 1697 Fragments arranged by size: 3831 2109 1697 251 226 152 AseI AT'TA_AT Cuts at: 160 1032 6820 160 Size: 872 5788 1606 Fragments arranged by size: 5788 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8266 AvaI C'yCGr_G Cuts at: 1870 1943 2355 2792 3231 3401 3705 4505 4828 Size: 73 412 437 439 170 304 800 323 Cuts at: 4828 5591 1870 Size: 763 4545 Fragments arranged by size: 4545 800 763 439 437 412 323 304 170 73 BamHI G'GATC_C Cuts at: 3354 4329 5596 5963 3354 Size: 975 1267 367 5657 Fragments arranged by size: 5657 1267 975 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2803 3481 4253 4298 4406 Size: 325 217 627 1016 678 772 45 108 Cuts at: 4406 5587 7042 618 Size: 1181 1455 1842 Fragments arranged by size: 1842 1455 1181 1016 772 678 627 325 217 108 45 BanII G_rGCy'C Cuts at: 1391 2062 2130 2385 2874 2937 3706 4512 5315 Size: 671 68 255 489 63 769 806 803 Cuts at: 5315 5337 5585 1391 Size: 22 248 4072 Fragments arranged by size: 4072 806 803 769 671 489 255 248 68 63 22 BbeI G_GCGC'C Cuts at: 4302 4410 4302 Size: 108 8158 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2633 2707 5519 928 Size: 971 734 74 2812 3675 Fragments arranged by size: 3675 2812 971 734 74 BbvCI CC'TCA_GC Cuts at: 2163 3617 3731 4169 2163 Size: 1454 114 438 6260 Fragments arranged by size: 6260 1454 438 114 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6457 6491 1903 Size: 34 50 34 4436 34 3678 Fragments arranged by size: 4436 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6158 7368 6158 Size: 1210 7056 BclI T'GATC_A Cuts at: 1548 1548 Size: 8266 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4802 6877 136 Size: 107 122 71 631 605 3130 2075 1525 Fragments arranged by size: 3130 2075 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4422 4708 5022 8261 4422 Size: 286 314 3239 4427 Fragments arranged by size: 4427 3239 314 286 BlpI GC'TnA_GC Cuts at: 3218 3740 5683 3218 Size: 522 1943 5801 Fragments arranged by size: 5801 1943 522 Bme1580I G_kGCm'C Cuts at: 1790 2385 2832 2937 3800 4125 4256 4950 5151 Size: 595 447 105 863 325 131 694 201 Cuts at: 5151 6327 7483 1790 Size: 1176 1156 2573 Fragments arranged by size: 2573 1176 1156 863 694 595 447 325 201 131 105 BmgBI CAC'GTC Cuts at: 1318 2494 1318 Size: 1176 7090 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2029 2113 5997 Size: 416 53 83 399 24 612 84 3884 Cuts at: 5997 6955 442 Size: 958 1753 Fragments arranged by size: 3884 1753 958 612 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8266 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2419 3941 2419 Size: 1522 6744 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2630 3233 3277 3392 3448 3572 4199 4291 Size: 1391 603 44 115 56 124 627 92 Cuts at: 4291 4439 5287 6926 1239 Size: 148 848 1639 2579 Fragments arranged by size: 2579 1639 1391 848 627 603 148 124 115 92 56 44 Bpu10I CC'TnA_GC Cuts at: 2163 2241 3617 3731 4169 4376 5349 2163 Size: 78 1376 114 438 207 973 5080 Fragments arranged by size: 5080 1376 973 438 207 114 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4784 5351 6391 7256 7554 4784 Size: 567 1040 865 298 5496 Fragments arranged by size: 5496 1040 865 567 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4003 5064 6929 882 Size: 617 2504 1061 1865 2219 Fragments arranged by size: 2504 2219 1865 1061 617 BsaAI yAC'GTr Cuts at: 493 1204 3160 4072 4945 6057 493 Size: 711 1956 912 873 1112 2702 Fragments arranged by size: 2702 1956 1112 912 873 711 BsaBI GATnn'nnATC Cuts at: 3341 8260 3341 Size: 4919 3347 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4029 4299 4407 4632 Size: 53 83 186 936 2496 270 108 225 Cuts at: 4632 4661 5471 6073 6455 7090 275 Size: 29 810 602 382 635 1451 Fragments arranged by size: 2496 1451 936 810 635 602 382 270 225 186 108 83 53 29 BsaWI w'CCGG_w Cuts at: 2331 6699 7097 7371 7518 7929 2331 Size: 4368 398 274 147 411 2668 Fragments arranged by size: 4368 2668 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3107 3137 5219 5249 5285 Size: 30 165 30 1704 30 2082 30 36 Cuts at: 5285 5315 1178 Size: 30 4129 Fragments arranged by size: 4129 2082 1704 165 36 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2196 2537 2540 2627 3008 3011 3026 3869 Size: 1010 341 3 87 381 3 15 843 Cuts at: 3869 4328 1186 Size: 459 5124 Fragments arranged by size: 5124 1010 843 459 381 341 87 15 3 3 BseYI C'CCAG_C Cuts at: 2061 2440 2757 2930 3468 3558 3575 3774 4149 Size: 379 317 173 538 90 17 199 375 Cuts at: 4149 4237 4818 4998 5016 5457 7469 7965 2061 Size: 88 581 180 18 441 2012 496 2362 Fragments arranged by size: 2362 2012 581 538 496 441 379 375 317 199 180 173 90 88 18 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2084 2555 3404 5030 1163 Size: 573 348 471 849 1626 4399 Fragments arranged by size: 4399 1626 849 573 471 348 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2520 3288 5716 6477 7505 1540 Size: 275 186 519 768 2428 761 1028 2301 Fragments arranged by size: 2428 2301 1028 768 761 519 275 186 BsiHKAI G_wGCw'C Cuts at: 3177 3434 3927 4080 4878 5337 6327 6412 7483 Size: 257 493 153 798 459 990 85 1071 Cuts at: 7483 3177 Size: 3960 Fragments arranged by size: 3960 1071 990 798 493 459 257 153 85 BsmI GAATG_Cn' Cuts at: 4355 5777 5870 4355 Size: 1422 93 6751 Fragments arranged by size: 6751 1422 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2130 2385 2832 2874 2937 3177 Size: 399 272 68 255 447 42 63 240 Cuts at: 3177 3434 3706 3800 3927 4080 4125 4256 4512 Size: 257 272 94 127 153 45 131 256 Cuts at: 4512 4878 4950 5151 5315 5337 5585 6327 6412 Size: 366 72 201 164 22 248 742 85 Cuts at: 6412 7483 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 447 399 366 272 272 257 256 255 248 240 201 164 153 131 127 94 85 72 68 63 45 42 22 BspEI T'CCGG_A Cuts at: 2331 7929 2331 Size: 5598 2668 BspHI T'CATG_A Cuts at: 3987 5445 6155 7885 3987 Size: 1458 710 1730 4368 Fragments arranged by size: 4368 1730 1458 710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2712 4980 5621 844 Size: 1868 2268 641 3489 Fragments arranged by size: 3489 2268 1868 641 BsrBI CCG'CTC Cuts at: 1394 1823 2797 4111 4318 4827 6153 1394 Size: 429 974 1314 207 509 1326 3507 Fragments arranged by size: 3507 1326 1314 974 509 429 207 BsrDI GCAATG_nn' Cuts at: 65 1248 2181 5343 6761 6935 65 Size: 1183 933 3162 1418 174 1396 Fragments arranged by size: 3162 1418 1396 1183 933 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2362 2664 3097 3192 3564 4497 4657 Size: 110 552 302 433 95 372 933 160 Cuts at: 4657 6910 7097 1700 Size: 2253 187 2869 Fragments arranged by size: 2869 2253 933 552 433 372 302 187 160 110 95 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8266 BssHII G'CGCG_C Cuts at: 1925 5081 1925 Size: 3156 5110 BssSI C'ACGA_G Cuts at: 2945 3311 3884 4871 5159 6326 7338 2945 Size: 366 573 987 288 1167 1012 3873 Fragments arranged by size: 3873 1167 1012 987 573 366 288 BstAPI GCAn_nnn'nTGC Cuts at: 794 3502 4501 794 Size: 2708 999 4559 Fragments arranged by size: 4559 2708 999 BstBI TT'CG_AA Cuts at: 7070 7070 Size: 8266 BstEII G'GTnAC_C Cuts at: 1184 5269 1184 Size: 4085 4181 BstXI CCAn_nnnn'nTGG Cuts at: 4417 4417 Size: 8266 BstYI r'GATC_y Cuts at: 1972 2280 2529 3354 4329 4422 4701 4708 5022 Size: 308 249 825 975 93 279 7 314 Cuts at: 5022 5596 5963 6348 6365 7817 7903 7915 8261 Size: 574 367 385 17 1452 86 12 346 Cuts at: 8261 1972 Size: 1977 Fragments arranged by size: 1977 1452 975 825 574 385 367 346 314 308 279 249 93 86 17 12 7 Bsu36I CC'TnA_GG Cuts at: 2843 3852 2843 Size: 1009 7257 BtgI C'CryG_G Cuts at: 513 1065 2067 2076 2596 3110 3326 3358 3901 Size: 552 1002 9 520 514 216 32 543 Cuts at: 3901 4125 4386 5408 513 Size: 224 261 1022 3371 Fragments arranged by size: 3371 1022 1002 552 543 520 514 261 224 216 32 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2136 3940 487 Size: 683 56 910 1804 4813 Fragments arranged by size: 4813 1804 910 683 56 BtsI GCAGTG_nn' Cuts at: 3037 5782 6576 6596 3037 Size: 2745 794 20 4707 Fragments arranged by size: 4707 2745 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8266 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8231 DraI TTT'AAA Cuts at: 5570 5570 Size: 8266 DraIII CAC_nnn'GTG Cuts at: 2950 3163 3316 2950 Size: 213 153 7900 Fragments arranged by size: 7900 213 153 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5111 5470 7273 809 Size: 507 3795 359 1803 1802 Fragments arranged by size: 3795 1803 1802 507 359 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2619 2984 Size: 54 1425 50 275 18 198 591 365 Cuts at: 2984 3091 3285 4158 4213 4527 5713 6602 8231 Size: 107 194 873 55 314 1186 889 1629 Cuts at: 8231 8 Size: 43 Fragments arranged by size: 1629 1425 1186 889 873 591 365 314 275 198 194 107 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3285 5713 1537 Size: 275 1473 2428 4090 Fragments arranged by size: 4090 2428 1473 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 5015 6196 1353 Size: 884 2778 1181 3423 Fragments arranged by size: 3423 2778 1181 884 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2582 2651 3266 6853 7227 7373 Size: 1064 138 993 69 615 3587 374 146 Cuts at: 7373 387 Size: 1280 Fragments arranged by size: 3587 1280 1064 993 615 374 146 138 69 EcoICRI GAG'CTC Cuts at: 5335 5335 Size: 8266 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2630 3233 3277 3392 3448 3572 Size: 393 476 522 603 44 115 56 124 Cuts at: 3572 4199 4291 4439 5243 5287 5531 5532 6329 Size: 627 92 148 804 44 244 1 797 Cuts at: 6329 6926 7713 7981 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 804 797 787 627 603 597 522 476 393 268 244 148 124 115 92 56 44 44 1 EcoNI CCTnn'n_nnAGG Cuts at: 2612 5138 2612 Size: 2526 5740 EcoO109I rG'GnC_Cy Cuts at: 2382 2934 3540 3847 3877 4441 5542 2382 Size: 552 606 307 30 564 1101 5106 Fragments arranged by size: 5106 1101 606 564 552 307 30 EcoRI G'AATT_C Cuts at: 5575 5953 5575 Size: 378 7888 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2650 4756 2650 Size: 2106 6160 FspI TGC'GCA Cuts at: 4859 6772 4859 Size: 1913 6353 HaeII r_GCGC'y Cuts at: 1678 2688 3245 4167 4302 4410 4690 7413 1678 Size: 1010 557 922 135 108 280 2723 2531 Fragments arranged by size: 2723 2531 1010 922 557 280 135 108 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 2483 2515 3623 Size: 32 135 32 857 32 22 32 1108 Cuts at: 3623 3655 4444 4476 4693 4725 5243 5275 6907 Size: 32 789 32 217 32 518 32 1632 Cuts at: 6907 6939 6981 7013 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1632 1108 857 789 518 217 135 42 32 32 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 2517 3757 5610 5856 669 Size: 988 281 579 1240 1853 246 3079 Fragments arranged by size: 3079 1853 1240 988 579 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8266 HpaI GTT'AAC Cuts at: 5856 5856 Size: 8266 KasI G'GCGC_C Cuts at: 4298 4406 4298 Size: 108 8158 KpnI G_GTAC'C Cuts at: 5591 5591 Size: 8266 MfeI C'AATT_G Cuts at: 5865 5865 Size: 8266 MluI A'CGCG_T Cuts at: 8142 8142 Size: 8266 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4644 4781 7380 7564 1305 Size: 3339 137 2599 184 2007 Fragments arranged by size: 3339 2599 2007 184 137 MscI TGG'CCA Cuts at: 10 64 1489 1832 2030 2621 2986 4160 4529 Size: 54 1425 343 198 591 365 1174 369 Cuts at: 4529 10 Size: 3747 Fragments arranged by size: 3747 1425 1174 591 369 365 343 198 54 MslI CAynn'nnrTG Cuts at: 518 1166 2503 3158 3692 4203 4385 6224 6583 Size: 648 1337 655 534 511 182 1839 359 Cuts at: 6583 6742 518 Size: 159 2042 Fragments arranged by size: 2042 1839 1337 655 648 534 511 359 182 159 MspA1I CmG'CkG Cuts at: 1606 2162 3112 3778 3843 4156 4525 4930 5002 Size: 556 950 666 65 313 369 405 72 Cuts at: 5002 5682 6359 7507 7752 1606 Size: 680 677 1148 245 2120 Fragments arranged by size: 2120 1148 950 680 677 666 556 405 369 313 245 72 65 NaeI GCC'GGC Cuts at: 2666 3099 2666 Size: 433 7833 NarI GG'CG_CC Cuts at: 4299 4407 4299 Size: 108 8158 NcoI C'CATG_G Cuts at: 513 1065 2067 2076 2596 4125 4386 513 Size: 552 1002 9 520 1529 261 4393 Fragments arranged by size: 4393 1529 1002 552 520 261 9 NdeI CA'TA_TG Cuts at: 387 387 Size: 8266 NgoMIV G'CCGG_C Cuts at: 2664 3097 2664 Size: 433 7833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8266 NotI GC'GGCC_GC Cuts at: 5713 5713 Size: 8266 NspI r_CATG'y Cuts at: 1132 1229 3496 5624 7169 1132 Size: 97 2267 2128 1545 2229 Fragments arranged by size: 2267 2229 2128 1545 97 PasI CC'CwG_GG Cuts at: 1348 2130 4951 1348 Size: 782 2821 4663 Fragments arranged by size: 4663 2821 782 PciI A'CATG_T Cuts at: 7165 7165 Size: 8266 PflMI CCAn_nnn'nTGG Cuts at: 1108 2068 2764 3316 4131 4156 1108 Size: 960 696 552 815 25 5218 Fragments arranged by size: 5218 960 815 696 552 25 PfoI T'CCnGG_A Cuts at: 2433 2883 3144 3345 4416 2433 Size: 450 261 201 1071 6283 Fragments arranged by size: 6283 1071 450 261 201 PmeI GTTT'AAAC Cuts at: 5570 5570 Size: 8266 PmlI CAC'GTG Cuts at: 3160 3160 Size: 8266 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4637 4669 5219 5251 6335 6367 7848 7880 4637 Size: 32 550 32 1084 32 1481 32 5023 Fragments arranged by size: 5023 1481 1084 550 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3847 3877 3847 Size: 30 8236 PshAI GACnn'nnGTC Cuts at: 1613 3942 5421 1613 Size: 2329 1479 4458 Fragments arranged by size: 4458 2329 1479 PsiI TTA'TAA Cuts at: 5836 5836 Size: 8266 PspOMI G'GGCC_C Cuts at: 2381 2933 2381 Size: 552 7714 PspXI vC'TCGA_Gb Cuts at: 2792 2792 Size: 8266 PstI C_TGCA'G Cuts at: 830 4452 4614 4929 5618 830 Size: 3622 162 315 689 3478 Fragments arranged by size: 3622 3478 689 315 162 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8266 PvuII CAG'CTG Cuts at: 2162 3778 4156 4525 4930 5002 2162 Size: 1616 378 369 405 72 5426 Fragments arranged by size: 5426 1616 405 378 369 72 SacI G_AGCT'C Cuts at: 5337 5337 Size: 8266 SacII CC_GC'GG Cuts at: 3113 3113 Size: 8266 SalI G'TCGA_C Cuts at: 1936 2515 5608 1936 Size: 579 3093 4594 Fragments arranged by size: 4594 3093 579 SanDI GG'GwC_CC Cuts at: 3847 3847 Size: 8266 SapI GCTCTTCn'nnn_ Cuts at: 5015 5015 Size: 8266 SbfI CC_TGCA'GG Cuts at: 5618 5618 Size: 8266 ScaI AGT'ACT Cuts at: 5165 6514 8226 5165 Size: 1349 1712 5205 Fragments arranged by size: 5205 1712 1349 SexAI A'CCwGG_T Cuts at: 4191 4357 4191 Size: 166 8100 SfcI C'TryA_G Cuts at: 826 1045 2904 4448 4610 4925 5614 6749 7430 Size: 219 1859 1544 162 315 689 1135 681 Cuts at: 7430 7621 826 Size: 191 1471 Fragments arranged by size: 1859 1544 1471 1135 689 681 315 219 191 162 SfoI GGC'GCC Cuts at: 4300 4408 4300 Size: 108 8158 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8266 SgrAI Cr'CCGG_yG Cuts at: 1700 4657 1700 Size: 2957 5309 SmaI CCC'GGG Cuts at: 1872 3233 5593 1872 Size: 1361 2360 4545 Fragments arranged by size: 4545 2360 1361 SmlI C'TyrA_G Cuts at: 820 1017 1943 2792 4799 5330 6370 7271 7533 Size: 197 926 849 2007 531 1040 901 262 Cuts at: 7533 820 Size: 1553 Fragments arranged by size: 2007 1553 1040 926 901 849 531 262 197 SnaBI TAC'GTA Cuts at: 493 6057 493 Size: 5564 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8266 SphI G_CATG'C Cuts at: 1132 3496 5624 1132 Size: 2364 2128 3774 Fragments arranged by size: 3774 2364 2128 SspI AAT'ATT Cuts at: 5 52 6190 5 Size: 47 6138 2081 Fragments arranged by size: 6138 2081 47 StuI AGG'CCT Cuts at: 1517 2085 1517 Size: 568 7698 StyI C'CwwG_G Cuts at: 513 1065 2067 2076 2596 3072 4125 4386 4909 Size: 552 1002 9 520 476 1053 261 523 Cuts at: 4909 5139 5533 5705 513 Size: 230 394 172 3074 Fragments arranged by size: 3074 1053 1002 552 523 520 476 394 261 230 172 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2534 3170 3788 6293 6310 6463 6648 Size: 1255 954 636 618 2505 17 153 185 Cuts at: 6648 7093 325 Size: 445 1498 Fragments arranged by size: 2505 1498 1255 954 636 618 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4743 4887 5163 Size: 274 80 33 51 1443 2766 144 276 Cuts at: 5163 6512 8224 96 Size: 1349 1712 138 Fragments arranged by size: 2766 1712 1443 1349 276 274 144 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3107 3139 3191 3223 5285 5317 8226 8258 3107 Size: 32 52 32 2062 32 2909 32 3115 Fragments arranged by size: 3115 2909 2062 52 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8266 XbaI T'CTAG_A Cuts at: 5602 5602 Size: 8266 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2074 2074 Size: 8266 XhoI C'TCGA_G Cuts at: 1943 2792 1943 Size: 849 7417 XmaI C'CCGG_G Cuts at: 1870 3231 5591 1870 Size: 1361 2360 4545 Fragments arranged by size: 4545 2360 1361 XmnI GAAnn'nnTTC Cuts at: 2714 6395 2714 Size: 3681 4585 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4633 6074 276 Size: 53 83 186 936 3099 1441 2468 Fragments arranged by size: 3099 2468 1441 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PsrI RsrII SfiI SrfI SwaI