(Linear) (Six-base) MAPSORT of: KIEE1350.seq Check: 7904 from: 1 to: 3561 KIEE1350.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AatII G_ACGT'C Cuts at: 0 2630 3561 Size: 2630 931 AccI GT'mk_AC Cuts at: 0 511 3561 Size: 511 3050 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 3238 3526 3527 3561 Size: 103 3135 288 1 34 Fragments arranged by size: 3135 288 103 34 1 AfeI AGC'GCT Cuts at: 0 2683 3561 Size: 2683 878 AflIII A'CryG_T Cuts at: 0 1805 3561 Size: 1805 1756 AleI CACnn'nnGTG Cuts at: 0 1153 3561 Size: 1153 2408 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 739 2602 3103 3561 Size: 739 1863 501 458 Fragments arranged by size: 1863 739 501 458 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3214 3246 3561 Size: 3214 32 315 Fragments arranged by size: 3214 315 32 AlwNI CAG_nnn'CTG Cuts at: 0 907 961 2151 2544 3000 3561 Size: 907 54 1190 393 456 561 Fragments arranged by size: 1190 907 561 456 393 54 ApaI G_GGCC'C Cuts at: 0 380 932 3561 Size: 380 552 2629 Fragments arranged by size: 2629 552 380 ApoI r'AATT_y Cuts at: 0 3319 3561 Size: 3319 242 AvaI C'yCGr_G Cuts at: 0 350 787 1226 1396 1700 2500 2823 3561 Size: 350 437 439 170 304 800 323 738 Fragments arranged by size: 800 738 439 437 350 323 304 170 BamHI G'GATC_C Cuts at: 0 1349 2324 3561 Size: 1349 975 1237 Fragments arranged by size: 1349 1237 975 BanI G'GyrC_C Cuts at: 0 798 1476 2248 2293 2401 3561 Size: 798 678 772 45 108 1160 Fragments arranged by size: 1160 798 772 678 108 45 BanII G_rGCy'C Cuts at: 0 57 125 380 869 932 1701 2507 3310 Size: 57 68 255 489 63 769 806 803 Cuts at: 3310 3332 3561 Size: 22 229 Fragments arranged by size: 806 803 769 489 255 229 68 63 57 22 BbeI G_GCGC'C Cuts at: 0 2297 2405 3561 Size: 2297 108 1156 Fragments arranged by size: 2297 1156 108 BbsI GAAGACnn'nnnn_ Cuts at: 0 628 702 3514 3561 Size: 628 74 2812 47 Fragments arranged by size: 2812 628 74 47 BbvCI CC'TCA_GC Cuts at: 0 158 1612 1726 2164 3561 Size: 158 1454 114 438 1397 Fragments arranged by size: 1454 1397 438 158 114 BglI GCCn_nnn'nGGC Cuts at: 0 2797 3561 Size: 2797 764 BglII A'GATC_T Cuts at: 0 2417 2703 3017 3561 Size: 2417 286 314 544 Fragments arranged by size: 2417 544 314 286 BlpI GC'TnA_GC Cuts at: 0 1213 1735 3561 Size: 1213 522 1826 Fragments arranged by size: 1826 1213 522 Bme1580I G_kGCm'C Cuts at: 0 380 827 932 1795 2120 2251 2945 3146 Size: 380 447 105 863 325 131 694 201 Cuts at: 3146 3561 Size: 415 Fragments arranged by size: 863 694 447 415 380 325 201 131 105 BmgBI CAC'GTC Cuts at: 0 489 3561 Size: 489 3072 BmrI ACTGGGnnnn_n' Cuts at: 0 24 108 3561 Size: 24 84 3453 Fragments arranged by size: 3453 84 24 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 414 1936 3561 Size: 414 1522 1625 Fragments arranged by size: 1625 1522 414 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 625 1228 1272 1387 1443 1567 2194 2286 Size: 625 603 44 115 56 124 627 92 Cuts at: 2286 2434 3282 3561 Size: 148 848 279 Fragments arranged by size: 848 627 625 603 279 148 124 115 92 56 44 Bpu10I CC'TnA_GC Cuts at: 0 158 236 1612 1726 2164 2371 3344 3561 Size: 158 78 1376 114 438 207 973 217 Fragments arranged by size: 1376 973 438 217 207 158 114 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2779 3346 3561 Size: 2779 567 215 Fragments arranged by size: 2779 567 215 BsaI GGTCTCn'nnnn_ Cuts at: 0 1998 3059 3561 Size: 1998 1061 502 Fragments arranged by size: 1998 1061 502 BsaAI yAC'GTr Cuts at: 0 1155 2067 2940 3561 Size: 1155 912 873 621 Fragments arranged by size: 1155 912 873 621 BsaBI GATnn'nnATC Cuts at: 0 1336 3561 Size: 1336 2225 BsaHI Gr'CG_yC Cuts at: 0 2024 2294 2402 2627 2656 3466 3561 Size: 2024 270 108 225 29 810 95 Fragments arranged by size: 2024 810 270 225 108 95 29 BsaWI w'CCGG_w Cuts at: 0 326 3561 Size: 326 3235 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1102 1132 3214 3244 3280 3310 3561 Size: 1102 30 2082 30 36 30 251 Fragments arranged by size: 2082 1102 251 36 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 191 532 535 622 1003 1006 1021 1864 Size: 191 341 3 87 381 3 15 843 Cuts at: 1864 2323 3561 Size: 459 1238 Fragments arranged by size: 1238 843 459 381 341 191 87 15 3 3 BseYI C'CCAG_C Cuts at: 0 56 435 752 925 1463 1553 1570 1769 Size: 56 379 317 173 538 90 17 199 Cuts at: 1769 2144 2232 2813 2993 3011 3452 3561 Size: 375 88 581 180 18 441 109 Fragments arranged by size: 581 538 441 379 375 317 199 180 173 109 90 88 56 18 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 550 1399 3025 3561 Size: 79 471 849 1626 536 Fragments arranged by size: 1626 849 536 471 79 BsiEI CG_ry'CG Cuts at: 0 515 1283 3561 Size: 515 768 2278 Fragments arranged by size: 2278 768 515 BsiHKAI G_wGCw'C Cuts at: 0 1172 1429 1922 2075 2873 3332 3561 Size: 1172 257 493 153 798 459 229 Fragments arranged by size: 1172 798 493 459 257 229 153 BsmI GAATG_Cn' Cuts at: 0 2350 3561 Size: 2350 1211 Bsp1286I G_dGCh'C Cuts at: 0 57 125 380 827 869 932 1172 1429 Size: 57 68 255 447 42 63 240 257 Cuts at: 1429 1701 1795 1922 2075 2120 2251 2507 2873 Size: 272 94 127 153 45 131 256 366 Cuts at: 2873 2945 3146 3310 3332 3561 Size: 72 201 164 22 229 Fragments arranged by size: 447 366 272 257 256 255 240 229 201 164 153 131 127 94 72 68 63 57 45 42 22 BspEI T'CCGG_A Cuts at: 0 326 3561 Size: 326 3235 BspHI T'CATG_A Cuts at: 0 1982 3440 3561 Size: 1982 1458 121 Fragments arranged by size: 1982 1458 121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 707 2975 3561 Size: 707 2268 586 Fragments arranged by size: 2268 707 586 BsrBI CCG'CTC Cuts at: 0 792 2106 2313 2822 3561 Size: 792 1314 207 509 739 Fragments arranged by size: 1314 792 739 509 207 BsrDI GCAATG_nn' Cuts at: 0 176 3338 3561 Size: 176 3162 223 Fragments arranged by size: 3162 223 176 BsrFI r'CCGG_y Cuts at: 0 357 659 1092 1187 1559 2492 2652 3561 Size: 357 302 433 95 372 933 160 909 Fragments arranged by size: 933 909 433 372 357 302 160 95 BssHII G'CGCG_C Cuts at: 0 3076 3561 Size: 3076 485 BssSI C'ACGA_G Cuts at: 0 940 1306 1879 2866 3154 3561 Size: 940 366 573 987 288 407 Fragments arranged by size: 987 940 573 407 366 288 BstAPI GCAn_nnn'nTGC Cuts at: 0 1497 2496 3561 Size: 1497 999 1065 Fragments arranged by size: 1497 1065 999 BstEII G'GTnAC_C Cuts at: 0 3264 3561 Size: 3264 297 BstXI CCAn_nnnn'nTGG Cuts at: 0 2412 3561 Size: 2412 1149 BstYI r'GATC_y Cuts at: 0 275 524 1349 2324 2417 2696 2703 3017 Size: 275 249 825 975 93 279 7 314 Cuts at: 3017 3561 Size: 544 Fragments arranged by size: 975 825 544 314 279 275 249 93 7 Bsu36I CC'TnA_GG Cuts at: 0 838 1847 3561 Size: 838 1009 1714 Fragments arranged by size: 1714 1009 838 BtgI C'CryG_G Cuts at: 0 62 71 591 1105 1321 1353 1896 2120 Size: 62 9 520 514 216 32 543 224 Cuts at: 2120 2381 3403 3561 Size: 261 1022 158 Fragments arranged by size: 1022 543 520 514 261 224 216 158 62 32 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 131 1935 3561 Size: 131 1804 1626 Fragments arranged by size: 1804 1626 131 BtsI GCAGTG_nn' Cuts at: 0 1032 3561 Size: 1032 2529 DraIII CAC_nnn'GTG Cuts at: 0 945 1158 1311 3561 Size: 945 213 153 2250 Fragments arranged by size: 2250 945 213 153 DrdI GACnn_nn'nnGTC Cuts at: 0 3106 3465 3561 Size: 3106 359 96 Fragments arranged by size: 3106 359 96 EaeI y'GGCC_r Cuts at: 0 23 614 979 1086 1280 2153 2208 2522 Size: 23 591 365 107 194 873 55 314 Cuts at: 2522 3561 Size: 1039 Fragments arranged by size: 1039 873 591 365 314 194 107 55 23 EagI C'GGCC_G Cuts at: 0 1280 3561 Size: 1280 2281 EarI CTCTTCn'nnn_ Cuts at: 0 232 3010 3561 Size: 232 2778 551 Fragments arranged by size: 2778 551 232 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 577 646 1261 3561 Size: 577 69 615 2300 Fragments arranged by size: 2300 615 577 69 EcoICRI GAG'CTC Cuts at: 0 3330 3561 Size: 3330 231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 625 1228 1272 1387 1443 1567 2194 Size: 103 522 603 44 115 56 124 627 Cuts at: 2194 2286 2434 3238 3282 3526 3527 3561 Size: 92 148 804 44 244 1 34 Fragments arranged by size: 804 627 603 522 244 148 124 115 103 92 56 44 44 34 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 607 3133 3561 Size: 607 2526 428 Fragments arranged by size: 2526 607 428 EcoO109I rG'GnC_Cy Cuts at: 0 377 929 1535 1842 1872 2436 3537 3561 Size: 377 552 606 307 30 564 1101 24 Fragments arranged by size: 1101 606 564 552 377 307 30 24 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 645 2751 3561 Size: 645 2106 810 Fragments arranged by size: 2106 810 645 FspI TGC'GCA Cuts at: 0 2854 3561 Size: 2854 707 HaeII r_GCGC'y Cuts at: 0 683 1240 2162 2297 2405 2685 3561 Size: 683 557 922 135 108 280 876 Fragments arranged by size: 922 876 683 557 280 135 108 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 424 456 478 510 1618 1650 2439 2471 Size: 424 32 22 32 1108 32 789 32 Cuts at: 2471 2688 2720 3238 3270 3561 Size: 217 32 518 32 291 Fragments arranged by size: 1108 789 518 424 291 217 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 0 512 1752 3561 Size: 512 1240 1809 Fragments arranged by size: 1809 1240 512 KasI G'GCGC_C Cuts at: 0 2293 2401 3561 Size: 2293 108 1160 Fragments arranged by size: 2293 1160 108 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2639 2776 3561 Size: 2639 137 785 Fragments arranged by size: 2639 785 137 MscI TGG'CCA Cuts at: 0 25 616 981 2155 2524 3561 Size: 25 591 365 1174 369 1037 Fragments arranged by size: 1174 1037 591 369 365 25 MslI CAynn'nnrTG Cuts at: 0 498 1153 1687 2198 2380 3561 Size: 498 655 534 511 182 1181 Fragments arranged by size: 1181 655 534 511 498 182 MspA1I CmG'CkG Cuts at: 0 157 1107 1773 1838 2151 2520 2925 2997 Size: 157 950 666 65 313 369 405 72 Cuts at: 2997 3561 Size: 564 Fragments arranged by size: 950 666 564 405 369 313 157 72 65 NaeI GCC'GGC Cuts at: 0 661 1094 3561 Size: 661 433 2467 Fragments arranged by size: 2467 661 433 NarI GG'CG_CC Cuts at: 0 2294 2402 3561 Size: 2294 108 1159 Fragments arranged by size: 2294 1159 108 NcoI C'CATG_G Cuts at: 0 62 71 591 2120 2381 3561 Size: 62 9 520 1529 261 1180 Fragments arranged by size: 1529 1180 520 261 62 9 NgoMIV G'CCGG_C Cuts at: 0 659 1092 3561 Size: 659 433 2469 Fragments arranged by size: 2469 659 433 NspI r_CATG'y Cuts at: 0 1491 3561 Size: 1491 2070 PasI CC'CwG_GG Cuts at: 0 125 2946 3561 Size: 125 2821 615 Fragments arranged by size: 2821 615 125 PflMI CCAn_nnn'nTGG Cuts at: 0 63 759 1311 2126 2151 3561 Size: 63 696 552 815 25 1410 Fragments arranged by size: 1410 815 696 552 63 25 PfoI T'CCnGG_A Cuts at: 0 428 878 1139 1340 2411 3561 Size: 428 450 261 201 1071 1150 Fragments arranged by size: 1150 1071 450 428 261 201 PmlI CAC'GTG Cuts at: 0 1155 3561 Size: 1155 2406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2632 2664 3214 3246 3561 Size: 2632 32 550 32 315 Fragments arranged by size: 2632 550 315 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1842 1872 3561 Size: 1842 30 1689 Fragments arranged by size: 1842 1689 30 PshAI GACnn'nnGTC Cuts at: 0 1937 3416 3561 Size: 1937 1479 145 Fragments arranged by size: 1937 1479 145 PspOMI G'GGCC_C Cuts at: 0 376 928 3561 Size: 376 552 2633 Fragments arranged by size: 2633 552 376 PspXI vC'TCGA_Gb Cuts at: 0 787 3561 Size: 787 2774 PstI C_TGCA'G Cuts at: 0 2447 2609 2924 3561 Size: 2447 162 315 637 Fragments arranged by size: 2447 637 315 162 PvuII CAG'CTG Cuts at: 0 157 1773 2151 2520 2925 2997 3561 Size: 157 1616 378 369 405 72 564 Fragments arranged by size: 1616 564 405 378 369 157 72 SacI G_AGCT'C Cuts at: 0 3332 3561 Size: 3332 229 SacII CC_GC'GG Cuts at: 0 1108 3561 Size: 1108 2453 SalI G'TCGA_C Cuts at: 0 510 3561 Size: 510 3051 SanDI GG'GwC_CC Cuts at: 0 1842 3561 Size: 1842 1719 SapI GCTCTTCn'nnn_ Cuts at: 0 3010 3561 Size: 3010 551 ScaI AGT'ACT Cuts at: 0 3160 3561 Size: 3160 401 SexAI A'CCwGG_T Cuts at: 0 2186 2352 3561 Size: 2186 166 1209 Fragments arranged by size: 2186 1209 166 SfcI C'TryA_G Cuts at: 0 899 2443 2605 2920 3561 Size: 899 1544 162 315 641 Fragments arranged by size: 1544 899 641 315 162 SfoI GGC'GCC Cuts at: 0 2295 2403 3561 Size: 2295 108 1158 Fragments arranged by size: 2295 1158 108 SgrAI Cr'CCGG_yG Cuts at: 0 2652 3561 Size: 2652 909 SmaI CCC'GGG Cuts at: 0 1228 3561 Size: 1228 2333 SmlI C'TyrA_G Cuts at: 0 787 2794 3325 3561 Size: 787 2007 531 236 Fragments arranged by size: 2007 787 531 236 SphI G_CATG'C Cuts at: 0 1491 3561 Size: 1491 2070 StuI AGG'CCT Cuts at: 0 80 3561 Size: 80 3481 StyI C'CwwG_G Cuts at: 0 62 71 591 1067 2120 2381 2904 3134 Size: 62 9 520 476 1053 261 523 230 Cuts at: 3134 3528 3561 Size: 394 33 Fragments arranged by size: 1053 523 520 476 394 261 230 62 33 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 529 1165 1783 3561 Size: 529 636 618 1778 Fragments arranged by size: 1778 636 618 529 TatI w'GTAC_w Cuts at: 0 2738 2882 3158 3561 Size: 2738 144 276 403 Fragments arranged by size: 2738 403 276 144 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1102 1134 1186 1218 3280 3312 3561 Size: 1102 32 52 32 2062 32 249 Fragments arranged by size: 2062 1102 249 52 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 69 3561 Size: 69 3492 XhoI C'TCGA_G Cuts at: 0 787 3561 Size: 787 2774 XmaI C'CCGG_G Cuts at: 0 1226 3561 Size: 1226 2335 XmnI GAAnn'nnTTC Cuts at: 0 709 3561 Size: 709 2852 ZraI GAC'GTC Cuts at: 0 2628 3561 Size: 2628 933 Enzymes that do cut: AatII AccI AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SalI SanDI SapI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AgeI AhdI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BmtI BsiWI BsmBI BsrGI BstBI BstZ17I ClaI CspCI DraI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PsiI PsrI PvuI RsrII SbfI SfiI SgfI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI