(Circular) (Six-base) MAPSORT of: pFN21AB6317.seq Check: 324 from: 1 to: 5485 pFN21AB6317 5485 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3295 278 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2828 1937 Size: 891 4594 Acc65I G'GTAC_C Cuts at: 2806 2806 Size: 5485 AclI AA'CG_TT Cuts at: 1563 3612 3985 1563 Size: 2049 373 3063 Fragments arranged by size: 3063 2049 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2166 2463 2513 3548 4932 5200 1632 Size: 534 297 50 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1035 534 297 268 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5288 AflIII A'CryG_T Cuts at: 1203 4384 5361 1203 Size: 3181 977 1327 Fragments arranged by size: 3181 1327 977 AgeI A'CCGG_T Cuts at: 4316 4316 Size: 5485 AhdI GACnn_n'nnGTC Cuts at: 4214 4214 Size: 5485 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5485 AlwNI CAG_nnn'CTG Cuts at: 2225 3228 4800 2225 Size: 1003 1572 2910 Fragments arranged by size: 2910 1572 1003 ApaLI G'TGCA_C Cuts at: 3542 4698 3542 Size: 1156 4329 ApoI r'AATT_y Cuts at: 1493 2361 2401 2794 3020 3172 5281 1493 Size: 868 40 393 226 152 2109 1697 Fragments arranged by size: 2109 1697 868 393 226 152 40 AseI AT'TA_AT Cuts at: 160 1032 4039 160 Size: 872 3007 1606 Fragments arranged by size: 3007 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 AvaI C'yCGr_G Cuts at: 1870 1943 2810 1870 Size: 73 867 4545 Fragments arranged by size: 4545 867 73 BamHI G'GATC_C Cuts at: 2815 3182 2815 Size: 367 5118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2806 4261 618 Size: 325 217 627 1019 1455 1842 Fragments arranged by size: 1842 1455 1019 627 325 217 BanII G_rGCy'C Cuts at: 1391 2804 1391 Size: 1413 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4514 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3676 3710 1903 Size: 34 1739 34 3678 Fragments arranged by size: 3678 1739 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2144 3377 4587 2144 Size: 1233 1210 3042 Fragments arranged by size: 3042 1233 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5485 BfrBI ATG'CAT Cuts at: 2265 2265 Size: 5485 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4096 136 Size: 107 122 71 631 605 2424 1525 Fragments arranged by size: 2424 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5480 5480 Size: 5485 BlpI GC'TnA_GC Cuts at: 2902 2902 Size: 5485 Bme1580I G_kGCm'C Cuts at: 1790 3546 4702 1790 Size: 1756 1156 2573 Fragments arranged by size: 2573 1756 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5485 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3216 4174 442 Size: 416 53 83 399 24 1799 958 1753 Fragments arranged by size: 1799 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5485 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2310 4145 1239 Size: 1071 1835 2579 Fragments arranged by size: 2579 1835 1071 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2039 2177 3610 4475 4773 2039 Size: 138 1433 865 298 2751 Fragments arranged by size: 2751 1433 865 298 138 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4148 882 Size: 617 2649 2219 Fragments arranged by size: 2649 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3276 493 Size: 711 2072 2702 Fragments arranged by size: 2702 2072 711 BsaBI GATnn'nnATC Cuts at: 5479 5479 Size: 5485 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3292 3674 4309 275 Size: 53 83 186 936 1759 382 635 1451 Fragments arranged by size: 1759 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3918 4316 4590 4737 5148 3918 Size: 398 274 147 411 4255 Fragments arranged by size: 4255 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5260 Fragments arranged by size: 5260 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5485 BseYI C'CCAG_C Cuts at: 2458 4688 5184 2458 Size: 2230 496 2759 Fragments arranged by size: 2759 2230 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4912 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2935 3696 4724 1540 Size: 275 186 934 761 1028 2301 Fragments arranged by size: 2301 1028 934 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3546 3631 4702 3546 Size: 85 1071 4329 Fragments arranged by size: 4329 1071 85 BsmI GAATG_Cn' Cuts at: 2267 2996 3089 2267 Size: 729 93 4663 Fragments arranged by size: 4663 729 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2804 3546 3631 4702 1391 Size: 399 1014 742 85 1071 2174 Fragments arranged by size: 2174 1071 1014 742 399 85 BspEI T'CCGG_A Cuts at: 5148 5148 Size: 5485 BspHI T'CATG_A Cuts at: 3374 5104 3374 Size: 1730 3755 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2131 2840 844 Size: 1287 709 3489 Fragments arranged by size: 3489 1287 709 BsrBI CCG'CTC Cuts at: 1394 1823 2047 3372 1394 Size: 429 224 1325 3507 Fragments arranged by size: 3507 1325 429 224 BsrDI GCAATG_nn' Cuts at: 65 1248 3980 4154 65 Size: 1183 2732 174 1396 Fragments arranged by size: 2732 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4129 4316 1700 Size: 110 2319 187 2869 Fragments arranged by size: 2869 2319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5485 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5485 BssSI C'ACGA_G Cuts at: 3545 4557 3545 Size: 1012 4473 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5485 BstBI TT'CG_AA Cuts at: 4289 4289 Size: 5485 BstEII G'GTnAC_C Cuts at: 1184 2179 1184 Size: 995 4490 BstXI CCAn_nnnn'nTGG Cuts at: 2288 2288 Size: 5485 BstYI r'GATC_y Cuts at: 1972 2815 3182 3567 3584 5036 5122 5134 5480 Size: 843 367 385 17 1452 86 12 346 Cuts at: 5480 1972 Size: 1977 Fragments arranged by size: 1977 1452 843 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4933 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4746 Fragments arranged by size: 4746 683 56 BtsI GCAGTG_nn' Cuts at: 3001 3795 3815 3001 Size: 794 20 4671 Fragments arranged by size: 4671 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5485 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2378 2413 529 Size: 35 1814 35 3601 Fragments arranged by size: 3601 1814 35 35 DraI TTT'AAA Cuts at: 2789 2789 Size: 5485 DraIII CAC_nnn'GTG Cuts at: 2104 2104 Size: 5485 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4492 809 Size: 507 3176 1802 Fragments arranged by size: 3176 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2932 3821 5450 Size: 54 1425 50 275 18 1102 889 1629 Cuts at: 5450 8 Size: 43 Fragments arranged by size: 1629 1425 1102 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2932 1537 Size: 275 1120 4090 Fragments arranged by size: 4090 1120 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3415 1353 Size: 2062 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4072 4446 4592 387 Size: 1064 138 2483 374 146 1280 Fragments arranged by size: 2483 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2166 2310 2463 2513 3548 4145 4932 Size: 393 534 144 153 50 1035 597 787 Cuts at: 4932 5200 1239 Size: 268 1524 Fragments arranged by size: 1524 1035 787 597 534 393 268 153 144 50 EcoRI G'AATT_C Cuts at: 2401 2794 3172 2401 Size: 393 378 4714 Fragments arranged by size: 4714 393 378 EcoRV GAT'ATC Cuts at: 2479 2479 Size: 5485 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2171 2171 Size: 5485 FspI TGC'GCA Cuts at: 3991 3991 Size: 5485 HaeII r_GCGC'y Cuts at: 1678 4632 1678 Size: 2954 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2173 2205 2404 2436 2438 Size: 32 135 32 601 32 199 32 2 Cuts at: 2438 2470 2525 2557 2597 2629 4126 4158 4200 Size: 32 55 32 40 32 1497 32 42 Cuts at: 4200 4232 1373 Size: 32 2626 Fragments arranged by size: 2626 1497 601 199 135 55 42 40 32 32 32 32 32 32 32 32 32 2 HincII GTy'rAC Cuts at: 669 1657 1938 2829 3075 669 Size: 988 281 891 246 3079 Fragments arranged by size: 3079 988 891 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5485 HpaI GTT'AAC Cuts at: 3075 3075 Size: 5485 KpnI G_GTAC'C Cuts at: 2810 2810 Size: 5485 MfeI C'AATT_G Cuts at: 3084 3084 Size: 5485 MluI A'CGCG_T Cuts at: 5361 5361 Size: 5485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4599 4783 1305 Size: 3294 184 2007 Fragments arranged by size: 3294 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3663 Fragments arranged by size: 3663 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3443 3802 3961 518 Size: 648 2277 359 159 2042 Fragments arranged by size: 2277 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2018 2755 2901 3578 4726 4971 1606 Size: 412 737 146 677 1148 245 2120 Fragments arranged by size: 2120 1148 737 677 412 245 146 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4933 NdeI CA'TA_TG Cuts at: 387 387 Size: 5485 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5485 NotI GC'GGCC_GC Cuts at: 2932 2932 Size: 5485 NruI TCG'CGA Cuts at: 2043 2043 Size: 5485 NsiI A_TGCA'T Cuts at: 2267 2267 Size: 5485 NspI r_CATG'y Cuts at: 1132 1229 2843 4388 1132 Size: 97 1614 1545 2229 Fragments arranged by size: 2229 1614 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5485 PciI A'CATG_T Cuts at: 4384 4384 Size: 5485 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5485 PmeI GTTT'AAAC Cuts at: 2789 2789 Size: 5485 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3554 3586 5067 5099 3554 Size: 32 1481 32 3940 Fragments arranged by size: 3940 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5485 PsiI TTA'TAA Cuts at: 3055 3055 Size: 5485 PstI C_TGCA'G Cuts at: 830 2837 830 Size: 2007 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5485 PvuII CAG'CTG Cuts at: 2755 2755 Size: 5485 SalI G'TCGA_C Cuts at: 1936 2827 1936 Size: 891 4594 SbfI CC_TGCA'GG Cuts at: 2837 2837 Size: 5485 ScaI AGT'ACT Cuts at: 3733 5445 3733 Size: 1712 3773 SfcI C'TryA_G Cuts at: 826 1045 2833 3968 4649 4840 826 Size: 219 1788 1135 681 191 1471 Fragments arranged by size: 1788 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5485 SmaI CCC'GGG Cuts at: 1872 2812 1872 Size: 940 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2054 2156 3589 4490 4752 820 Size: 197 926 111 102 1433 901 262 1553 Fragments arranged by size: 1553 1433 926 901 262 197 111 102 SnaBI TAC'GTA Cuts at: 493 3276 493 Size: 2783 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5485 SphI G_CATG'C Cuts at: 1132 2843 1132 Size: 1711 3774 SspI AAT'ATT Cuts at: 5 52 3409 5 Size: 47 3357 2081 Fragments arranged by size: 3357 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5485 StyI C'CwwG_G Cuts at: 513 1065 2924 513 Size: 552 1859 3074 Fragments arranged by size: 3074 1859 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3512 3529 3682 3867 4312 325 Size: 1255 1932 17 153 185 445 1498 Fragments arranged by size: 1932 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2667 3731 5443 Size: 274 80 33 51 1443 690 1064 1712 Cuts at: 5443 96 Size: 138 Fragments arranged by size: 1712 1443 1064 690 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5445 5477 5445 Size: 32 5453 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5485 XbaI T'CTAG_A Cuts at: 2821 2821 Size: 5485 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5485 XmaI C'CCGG_G Cuts at: 1870 2810 1870 Size: 940 4545 XmnI GAAnn'nnTTC Cuts at: 3614 3614 Size: 5485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3293 276 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI