################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## Acc65I AccI AccIII AcsI AfaI AflII AgeI AocI ApoI AscI Asp700I Asp718I AspI AviII AvrII BanI BbeI BbrPI BbvCI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BsaWI BseAI BseRI BsiWI BsmBI BsmI Bsp1407I BspEI BspHI BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CpoI DraIII DrdI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HinfI HpaI KasI KpnI MaeI MamI MfeI MluI MroI MunI NarI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PvuI RcaI RsaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TfiI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AatII 1207 GACGTC GACGT^C AclI 679 AACGTT AA^CGTT AcyI 1207 GACGTC G[GA]^CG[CT]C AflIII 992 ACATGT A^C[GA][CT]GT Alw44I 194 GTGCAC G^TGCAC AlwNI 311 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1253 AGCGCT AGC^GCT ApaI 1165 GGGCCC GGGCC^C ApaLI 194 GTGCAC G^TGCAC AseI 1018 ATTAAT AT^TAAT AsnI 1018 ATTAAT AT^TAAT AspHI 194 GTGCAC G[AT]GC[AT]^C AvaI 1160 CCCGGG C^[CT]CG[GA]G BalI 138 TGGCCA TGG^CCA BamHI 1179 GGATCC G^GATCC BcgI 5 CGACCGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 662 GGATAC GTATCC(6/5) BlpI 1026 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1026 GCTCAGC GC^T[ACGT]AGC BsaHI 1207 GACGTC G[GA]^CG[CT]C BsaI 1005 GGTCTC GGTCTC(1/5) BsiEI 5 CGACCG CG[GA][CT]^CG BsiHKAI 194 GTGCAC G[AT]GC[AT]^C BsoBI 1160 CCCGGG C^[CT]CG[GA]G BspDI 717 ATCGAT AT^CGAT BspLU11I 992 ACATGT A^CATGT BsrFI 971 GCCGGC [GA]^CCGG[CT] BssHII 15 GCGCGC G^CGCGC CelII 1026 GCTCAGC GC^T[ACGT]AGC Cfr10I 971 GCCGGC [GA]^CCGG[CT] ClaI 717 ATCGAT AT^CGAT DdeI 1027 CTCAG C^T[ACGT]AG DraI 892 TTTAAA TTT^AAA EaeI 138 TGGCCA [CT]^GGCC[GA] Eco47III 1253 AGCGCT AGC^GCT EcoNI 1032 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1001 CCAAGG C^C[AT][AT]GG FauI 47 GCGGG CCCGC(4/6) Hin1I 1207 GACGTC G[GA]^CG[CT]C KspI 8 CCGCGG CCGC^GG MluNI 138 TGGCCA TGG^CCA MscI 138 TGGCCA TGG^CCA MslI 1017 CATTAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 971 GCCGGC GCC^GGC NdeI 641 CATATG CA^TATG NgoMIV 971 GCCGGC G^CCGGC NspI 992 ACATGT [GA]CATG^[CT] PflMI 1104 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 619 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1018 ATTAAT AT^TAAT Psp1406I 679 AACGTT AA^CGTT PvuII 1081 CAGCTG CAG^CTG SacII 8 CCGCGG CCGC^GG SapI 487 GAAGAGC GCTCTTC(1/4) SmaI 1160 CCCGGG CCC^GGG SspI 867 AATATT AAT^ATT StyI 1001 CCAAGG C^C[AT][AT]GG TaqI 718 TCGA T^CGA Tsp45I 230 GTCAC ^GT[GC]AC TthHB8I 718 TCGA T^CGA Van91I 1104 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1160 CCCGGG C^CCGGG XmaI 1160 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 9 CGCG CG^CG 16 CGCG AhdI 51 GACGGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 428 GACTGAATGTC AspEI 51 GACGGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 428 GACTGAATGTC BanII 1054 GGGCTC G[GA]GC[CT]^C 1165 GGGCCC BbsI 856 GAAGAC GAAGAC(2/6) 1233 GAAGAC BpmI 1059 CTCCAG CTGGAG(16/14) 323 CTGGAG BpuAI 856 GAAGAC GAAGAC(2/6) 1233 GAAGAC BsgI 1238 CTGCAC GTGCAG(16/14) 460 GTGCAG BspMI 55 GCAGGT ACCTGC(4/8) 760 GCAGGT BsrDI 468 CATTGC GCAATG(2/0) 134 GCAATG BstUI 9 CGCG CG^CG 16 CGCG BstYI 372 GGATCT [GA]^GATC[CT] 1179 GGATCC DsaI 8 CCGCGG C^C[GA][CT]GG 1050 CCGTGG Eam1105I 51 GACGGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 428 GACTGAATGTC HaeII 301 GGCGCT [GA]GCGC^[CT] 1253 AGCGCT HgaI 900 GACGC GACGC(5/10) 1153 GCGTC MaeII 680 ACGT A^CGT 1208 ACGT MaeIII 230 GTCAC ^GT[ACGT]AC 932 GTTAC MflI 372 GGATCT [GA]^GATC[CT] 1179 GGATCC MvnI 9 CGCG CG^CG 16 CGCG Tsp509I 275 AATT ^AATT 1270 AATT XhoII 372 GGATCT [GA]^GATC[CT] 1179 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI Tru9I TseI TspRI